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The map label for this gene is pleD [H]

Identifier: 159897665

GI number: 159897665

Start: 1297873

End: 1299621

Strand: Reverse

Name: pleD [H]

Synonym: Haur_1136

Alternate gene names: 159897665

Gene position: 1299621-1297873 (Counterclockwise)

Preceding gene: 159897667

Following gene: 159897657

Centisome position: 20.48

GC content: 44.83

Gene sequence:

>1749_bases
ATGGTAGTAACACTCCAAATCCAAGACGCATTAAAACAATCGACAAATCCTGCCCATACGATCGATTTATTGAATGTATG
GGCCGCAAATTTACGCCACACCGATTTAGCGCAAGCTTGGAAATTAAATTATCAAGCCGAACATCATGCTCGTAATGGCA
TGTTTGCCCATACTCCTTATTATCAAGGTTTAGTTGAATGTTGGTATTGGCGCGGCAAATTTTATAGCGTGCATAAAAAA
TATGCTGATGCACTTGGTTGTTTTTATAAAGCAATCGAATATTGTGATTATGTTGATGATGCATTACTTAGCTTTGAGAA
TCAGGCTGCTTGCTATTATCAAGATGCGCCCGATCAGTTTATTGAAAATTGGGTTGATAAATTAGCATTGCATAATATTC
TTGGCGTAACCTTAGCCTTGCAGTCGCAGCTTGATGCAGCTCTGGAGCATTATCTGATTGCTGAAGAAATTGCAATTATG
CACCAAAATGAATGGATGCAAACAATTCTCAATAGCAATATTGCCTATTTATATCGTGTGCTTGAGCAACCAATCGAGGC
CGAAAATTATTTGCAACGGGCTTTTCAGCGCTTACCCGAAGCTTTAGAAACTCAAGCCCAAAAACGTTTGTATGCCACGA
TGCTAGATAATACCTGTTGGGTTGCGCTTGAACATGGTCAATTGTACGATGCTTTAATCTATGCCAATGCCAGCTTGGAA
TTGTATCAAGCTCTGAATTGGTCGCAGGGCATTGTCGAGGTTTTGAATATTCTAGGGGTGGTTTATCAGCGATTTGATCA
AGCTGAACGGGCCATGGCCGAGTTTGAAGCCAGTTTTGAGTTAGCTCAACGCCTTGGCTTTCAAGAAGATATGATTTTTT
CCTTGATCCAGCGTGGTATGCTAGTAGCAACCCAACCGGATTACACCCAAGCCCTAACCTTGATTCACGCTGGTTTGGCC
TTGGCCTTGGATACCGATGATCTTAGCCAACAAGCCTTGGCTCATAAAGCCCTAGCCGATGTGTACGAACAAACCAACGC
TTGTGCCGAGGCCTTGTACCACTTCAAACACTACCATCAGATTCGCGAAAACCTGTTTAACGAGCGCTCAGATCAACGGT
TACGGCTATTGCAAGTGACACATCAAGTACTTCAGGCTCAACATCAAGTACGGTTTTATTATCAAAAAACACAGGAATTG
GTGGCTGAGATGCAAGAACGTGATCGCCAACGAATTGAATTGGAACGATTAGCAACGATCGATAGCTTAACTGGCTTATA
CAATCGTCGCCATTTTTTGGGCTTGGCTCAAAGCTTGTTGGAGCAGGCTTGGCAGGCTGGCAATGCTTTGGCCATTTTGC
TGTTTGATATTGATCATTTTAAGCAAATTAACGATCGCCATGGCCATCATATTGGCGATCAAATGTTGCAATTGGTGGCC
ACGACGGCCCAAGCAGCCTTGCGCCAATCTGATTTGCTTGGGCGTTATGGCGGTGAGGAATTTGTGGTGATGCTGACGCG
GGTGAGTAATGCCGAGGCGCTGTTGGTGGCCGAACGCTTGCGCAGCGCGATCGAACAGCTAATATTAACCGACAACCAGC
AATCGGTTACGACTTCGATTAGCGTTGGTTTGACGCTGAGCTACCCGCATGCTGAAATCCCCTTGGAGCGCTTGATTCAA
CAAGCCGACCATGCGCTCTACCAAGCCAAAGCCGCTGGCCGCAACAATGTGCAAATCTATTCAGCCTAA

Upstream 100 bases:

>100_bases
ATTATGCTTATGTTAAAGTTTTGTTATTGATTATAGGTTGATTGCTGAATCGCAATTTTAGCTATCTATAGAGCAAGAAG
CTTGCCAACGAAAGTTTACT

Downstream 100 bases:

>100_bases
ATTGATTGCGCTCACCCTCTAGTCTATAAGGGTAGTGTATTGTTCACCACCCTTCCGTCCCGCCTCAAGGCGGGAGACGC
AGGGGGCTTTAATCCCCCTG

Product: diguanylate cyclase

Products: NA

Alternate protein names: Stalked cell differentiation-controlling protein; Diguanylate cyclase; DGC; Diguanylate kinase [H]

Number of amino acids: Translated: 582; Mature: 582

Protein sequence:

>582_residues
MVVTLQIQDALKQSTNPAHTIDLLNVWAANLRHTDLAQAWKLNYQAEHHARNGMFAHTPYYQGLVECWYWRGKFYSVHKK
YADALGCFYKAIEYCDYVDDALLSFENQAACYYQDAPDQFIENWVDKLALHNILGVTLALQSQLDAALEHYLIAEEIAIM
HQNEWMQTILNSNIAYLYRVLEQPIEAENYLQRAFQRLPEALETQAQKRLYATMLDNTCWVALEHGQLYDALIYANASLE
LYQALNWSQGIVEVLNILGVVYQRFDQAERAMAEFEASFELAQRLGFQEDMIFSLIQRGMLVATQPDYTQALTLIHAGLA
LALDTDDLSQQALAHKALADVYEQTNACAEALYHFKHYHQIRENLFNERSDQRLRLLQVTHQVLQAQHQVRFYYQKTQEL
VAEMQERDRQRIELERLATIDSLTGLYNRRHFLGLAQSLLEQAWQAGNALAILLFDIDHFKQINDRHGHHIGDQMLQLVA
TTAQAALRQSDLLGRYGGEEFVVMLTRVSNAEALLVAERLRSAIEQLILTDNQQSVTTSISVGLTLSYPHAEIPLERLIQ
QADHALYQAKAAGRNNVQIYSA

Sequences:

>Translated_582_residues
MVVTLQIQDALKQSTNPAHTIDLLNVWAANLRHTDLAQAWKLNYQAEHHARNGMFAHTPYYQGLVECWYWRGKFYSVHKK
YADALGCFYKAIEYCDYVDDALLSFENQAACYYQDAPDQFIENWVDKLALHNILGVTLALQSQLDAALEHYLIAEEIAIM
HQNEWMQTILNSNIAYLYRVLEQPIEAENYLQRAFQRLPEALETQAQKRLYATMLDNTCWVALEHGQLYDALIYANASLE
LYQALNWSQGIVEVLNILGVVYQRFDQAERAMAEFEASFELAQRLGFQEDMIFSLIQRGMLVATQPDYTQALTLIHAGLA
LALDTDDLSQQALAHKALADVYEQTNACAEALYHFKHYHQIRENLFNERSDQRLRLLQVTHQVLQAQHQVRFYYQKTQEL
VAEMQERDRQRIELERLATIDSLTGLYNRRHFLGLAQSLLEQAWQAGNALAILLFDIDHFKQINDRHGHHIGDQMLQLVA
TTAQAALRQSDLLGRYGGEEFVVMLTRVSNAEALLVAERLRSAIEQLILTDNQQSVTTSISVGLTLSYPHAEIPLERLIQ
QADHALYQAKAAGRNNVQIYSA
>Mature_582_residues
MVVTLQIQDALKQSTNPAHTIDLLNVWAANLRHTDLAQAWKLNYQAEHHARNGMFAHTPYYQGLVECWYWRGKFYSVHKK
YADALGCFYKAIEYCDYVDDALLSFENQAACYYQDAPDQFIENWVDKLALHNILGVTLALQSQLDAALEHYLIAEEIAIM
HQNEWMQTILNSNIAYLYRVLEQPIEAENYLQRAFQRLPEALETQAQKRLYATMLDNTCWVALEHGQLYDALIYANASLE
LYQALNWSQGIVEVLNILGVVYQRFDQAERAMAEFEASFELAQRLGFQEDMIFSLIQRGMLVATQPDYTQALTLIHAGLA
LALDTDDLSQQALAHKALADVYEQTNACAEALYHFKHYHQIRENLFNERSDQRLRLLQVTHQVLQAQHQVRFYYQKTQEL
VAEMQERDRQRIELERLATIDSLTGLYNRRHFLGLAQSLLEQAWQAGNALAILLFDIDHFKQINDRHGHHIGDQMLQLVA
TTAQAALRQSDLLGRYGGEEFVVMLTRVSNAEALLVAERLRSAIEQLILTDNQQSVTTSISVGLTLSYPHAEIPLERLIQ
QADHALYQAKAAGRNNVQIYSA

Specific function: Catalyzes the condensation of two GTP molecules to the cyclic dinucleotide di-GMP (c-di-GMP), which acts as a secondary messenger [H]

COG id: COG2199

COG function: function code T; FOG: GGDEF domain

Gene ontology:

Cell location: Cytoplasm. Note=Phosphorylated PleD localizes to the differentiating pole [H]

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 response regulatory domains [H]

Homologues:

Organism=Escherichia coli, GI145693134, Length=164, Percent_Identity=40.8536585365854, Blast_Score=125, Evalue=1e-29,
Organism=Escherichia coli, GI1787262, Length=161, Percent_Identity=36.0248447204969, Blast_Score=110, Evalue=2e-25,
Organism=Escherichia coli, GI1786584, Length=186, Percent_Identity=36.0215053763441, Blast_Score=110, Evalue=3e-25,
Organism=Escherichia coli, GI87082007, Length=158, Percent_Identity=39.873417721519, Blast_Score=104, Evalue=1e-23,
Organism=Escherichia coli, GI87081881, Length=178, Percent_Identity=34.2696629213483, Blast_Score=103, Evalue=2e-23,
Organism=Escherichia coli, GI1788085, Length=168, Percent_Identity=34.5238095238095, Blast_Score=101, Evalue=2e-22,
Organism=Escherichia coli, GI1787802, Length=161, Percent_Identity=34.7826086956522, Blast_Score=97, Evalue=3e-21,
Organism=Escherichia coli, GI1787816, Length=164, Percent_Identity=32.9268292682927, Blast_Score=95, Evalue=1e-20,
Organism=Escherichia coli, GI1787541, Length=191, Percent_Identity=31.413612565445, Blast_Score=91, Evalue=2e-19,
Organism=Escherichia coli, GI1788381, Length=185, Percent_Identity=30.8108108108108, Blast_Score=80, Evalue=3e-16,
Organism=Escherichia coli, GI87081974, Length=127, Percent_Identity=30.7086614173228, Blast_Score=71, Evalue=2e-13,
Organism=Escherichia coli, GI87081977, Length=161, Percent_Identity=31.055900621118, Blast_Score=69, Evalue=1e-12,
Organism=Escherichia coli, GI1788956, Length=161, Percent_Identity=34.1614906832298, Blast_Score=67, Evalue=4e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR011006
- InterPro:   IPR000160
- InterPro:   IPR001789 [H]

Pfam domain/function: PF00990 GGDEF; PF00072 Response_reg [H]

EC number: =2.7.7.65 [H]

Molecular weight: Translated: 66824; Mature: 66824

Theoretical pI: Translated: 5.31; Mature: 5.31

Prosite motif: PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVVTLQIQDALKQSTNPAHTIDLLNVWAANLRHTDLAQAWKLNYQAEHHARNGMFAHTPY
CEEEEEEHHHHHCCCCCHHHHHHHHHHHHHCCHHHHHHHHHCCCCHHHHHCCCCEEECHH
YQGLVECWYWRGKFYSVHKKYADALGCFYKAIEYCDYVDDALLSFENQAACYYQDAPDQF
HHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEECCCHHHH
IENWVDKLALHNILGVTLALQSQLDAALEHYLIAEEIAIMHQNEWMQTILNSNIAYLYRV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHH
LEQPIEAENYLQRAFQRLPEALETQAQKRLYATMLDNTCWVALEHGQLYDALIYANASLE
HHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCEEHHHEECCCHH
LYQALNWSQGIVEVLNILGVVYQRFDQAERAMAEFEASFELAQRLGFQEDMIFSLIQRGM
HHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCE
LVATQPDYTQALTLIHAGLALALDTDDLSQQALAHKALADVYEQTNACAEALYHFKHYHQ
EEECCCCHHHHHHHHHHHHHEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IRENLFNERSDQRLRLLQVTHQVLQAQHQVRFYYQKTQELVAEMQERDRQRIELERLATI
HHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DSLTGLYNRRHFLGLAQSLLEQAWQAGNALAILLFDIDHFKQINDRHGHHIGDQMLQLVA
HHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEEHHHHHHHHCCCCCHHHHHHHHHHH
TTAQAALRQSDLLGRYGGEEFVVMLTRVSNAEALLVAERLRSAIEQLILTDNQQSVTTSI
HHHHHHHHHHHHHHHCCCCEEEEEEEECCCCHHHHHHHHHHHHHHHHHHCCCCHHHEEEE
SVGLTLSYPHAEIPLERLIQQADHALYQAKAAGRNNVQIYSA
EEEEEEECCCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEEEC
>Mature Secondary Structure
MVVTLQIQDALKQSTNPAHTIDLLNVWAANLRHTDLAQAWKLNYQAEHHARNGMFAHTPY
CEEEEEEHHHHHCCCCCHHHHHHHHHHHHHCCHHHHHHHHHCCCCHHHHHCCCCEEECHH
YQGLVECWYWRGKFYSVHKKYADALGCFYKAIEYCDYVDDALLSFENQAACYYQDAPDQF
HHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEECCCHHHH
IENWVDKLALHNILGVTLALQSQLDAALEHYLIAEEIAIMHQNEWMQTILNSNIAYLYRV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHH
LEQPIEAENYLQRAFQRLPEALETQAQKRLYATMLDNTCWVALEHGQLYDALIYANASLE
HHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCEEHHHEECCCHH
LYQALNWSQGIVEVLNILGVVYQRFDQAERAMAEFEASFELAQRLGFQEDMIFSLIQRGM
HHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCE
LVATQPDYTQALTLIHAGLALALDTDDLSQQALAHKALADVYEQTNACAEALYHFKHYHQ
EEECCCCHHHHHHHHHHHHHEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IRENLFNERSDQRLRLLQVTHQVLQAQHQVRFYYQKTQELVAEMQERDRQRIELERLATI
HHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DSLTGLYNRRHFLGLAQSLLEQAWQAGNALAILLFDIDHFKQINDRHGHHIGDQMLQLVA
HHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEEHHHHHHHHCCCCCHHHHHHHHHHH
TTAQAALRQSDLLGRYGGEEFVVMLTRVSNAEALLVAERLRSAIEQLILTDNQQSVTTSI
HHHHHHHHHHHHHHHCCCCEEEEEEEECCCCHHHHHHHHHHHHHHHHHHCCCCHHHEEEE
SVGLTLSYPHAEIPLERLIQQADHALYQAKAAGRNNVQIYSA
EEEEEEECCCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA