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The map label for this gene is liaS [H]

Identifier: 159897634

GI number: 159897634

Start: 1265195

End: 1266835

Strand: Reverse

Name: liaS [H]

Synonym: Haur_1105

Alternate gene names: 159897634

Gene position: 1266835-1265195 (Counterclockwise)

Preceding gene: 159897635

Following gene: 159897633

Centisome position: 19.96

GC content: 51.31

Gene sequence:

>1641_bases
GTGCAGCAGCATCGTAAATTAACCCTCGTTCAACGCTTTGGGCAGCGCTTTGGCACGCTACGCTGGAAACTCACATGGTC
GTATGTGTGGATCAGCGTAGCGTTGACTTTGTTGATTAATGGTTTGGCGTTTGGTTTAGTGATGGGTGCTGCTCCACCGC
TCAATGCTCCCGCCTTGCTTAGCGCCGCCCAAAATCATGCCGCTGAGTTTAGCGAGGTTTTGCAAGAGCCAACCGATCAG
CACACAATCCAACAGTTGTTACGCTATCGCTTTGGTTCGTTGCGCGGCCAATTTCTGCAAAATAACCTTGAATTAGCGAT
TAGCAATACTATCAATAATTCTCCCAGCGATCTTTCGCAGGAAATGATTTTGATGCCAGCTGAGGTATTTTCGCCTGAAG
CGACCAATGTGTTTAGCGAGAGCTACCACCTCTTATTGCTTGATGCGCAGGGTAATGTGCTCGGCGGGACATTTCCCAGC
CGTACTCCGGCGGGCCAGCCCTGGAACGATGCGATAGTGGGCAATGATCGACGGGTTGTGCAGGCAGCACTTGCTGGCAG
CGACGATATCGATCAATTAACTTGGAAGTATGAGAATTATTTAGTGATTGCCACTCCAGTTCGCGATCAAGCCAACCAAG
TGATTGGGGCACTGTATGTACGTTCGCGGCCACTTAGCCAAAATCAAGTGATTGTTGCATTGTTGATGTTTGTTATTTTT
ATTGCCAGTTCAATTGTTTCGATTATTGCCAATGCCTTAATTGGCATGGTCTATGGCTGGTTTGTGGCCCGTAATTTTGT
GCGCCGTTTGGTGCATCTCACCCAAGCTACCGATAGTTTGGCCGCTGGCGATTTGAGCGTGCGGGTCAACGATGGATCAA
TCGACGAAATTGGGCATTTGGCGCGGCGCTTCGACAGCATGGCCCAACAGCTTGAATCAAATGTCAAAATGTTACGTCAA
CTAGCTGATCGCAATGCTGCCTTAGTTGAGCAGGCAGGCCAATTGGCAATCGTCGAGGAGCGTAATCGGTTGGCTCGCGA
TTTGCACGATAGCGTTAGCCAAGAGTTATTTAGCGTCACGATGTTGGCGGCGGCTGCGCGTAATTTATTGCCAGCCCAGC
CCGATAAAGCGCGTAGCCAAGTTGAACAACTCAGCCAAATGGCCCAACGCGCCTTGCACGAAACTCGTGGCTTGATCTTC
GCACTTCGGCCTGCTGCGCTCGGTGATCAAGGTTTAGTCCCAGCATTACGTCAACTTACCGAAGAGGCAGCGCGTCGCCA
AGGCTTGCAGATTGAACTGAATACCAACGGCGAACGGCGCATTCCCTTAGATCATGAGCAGGCACTCTATCGGATTTGCC
AAGAAGCCTTGGCCAATGTGACCAAGCATAGCGGCGTGAACAGCGCCAGCGTAAGCCTTGAATATGAAGCCCATCGCACC
ACTTTAGAGGTGCGCGATCGTGGCCGTGGCTTTGATCAAGATAAACCGCGCAATTCGCACTCGCTAGGCTTGATTAGCAT
TCAAGAACGCGCCAAAGCAGTTGGCGGCACAGTTGAATTAACTGCTGCGCCAGGCCAAGGCACAAGCCTACGCATCGTTG
TACCACGAACCCAAACTGGGCTACTGGTCGAGCCACGTTGA

Upstream 100 bases:

>100_bases
CAACGCACCATGATCGCCAATTTCAATAACCCACAGCCCTACGATTTTCAACTTGGCTGTTTGATGATTGCAATCAGTAT
TTGGCGACAAGAGGCAGCGA

Downstream 100 bases:

>100_bases
TAGCCAAGAAGGAAAGCACCATGCAAGAGCAGATTCGAGTTGTTTTAGTTGATGATCATGTCGTGGTACGGGTAGGTTTA
CGTGCCTTCTTGGATACACA

Product: integral membrane sensor signal transduction histidine kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 546; Mature: 546

Protein sequence:

>546_residues
MQQHRKLTLVQRFGQRFGTLRWKLTWSYVWISVALTLLINGLAFGLVMGAAPPLNAPALLSAAQNHAAEFSEVLQEPTDQ
HTIQQLLRYRFGSLRGQFLQNNLELAISNTINNSPSDLSQEMILMPAEVFSPEATNVFSESYHLLLLDAQGNVLGGTFPS
RTPAGQPWNDAIVGNDRRVVQAALAGSDDIDQLTWKYENYLVIATPVRDQANQVIGALYVRSRPLSQNQVIVALLMFVIF
IASSIVSIIANALIGMVYGWFVARNFVRRLVHLTQATDSLAAGDLSVRVNDGSIDEIGHLARRFDSMAQQLESNVKMLRQ
LADRNAALVEQAGQLAIVEERNRLARDLHDSVSQELFSVTMLAAAARNLLPAQPDKARSQVEQLSQMAQRALHETRGLIF
ALRPAALGDQGLVPALRQLTEEAARRQGLQIELNTNGERRIPLDHEQALYRICQEALANVTKHSGVNSASVSLEYEAHRT
TLEVRDRGRGFDQDKPRNSHSLGLISIQERAKAVGGTVELTAAPGQGTSLRIVVPRTQTGLLVEPR

Sequences:

>Translated_546_residues
MQQHRKLTLVQRFGQRFGTLRWKLTWSYVWISVALTLLINGLAFGLVMGAAPPLNAPALLSAAQNHAAEFSEVLQEPTDQ
HTIQQLLRYRFGSLRGQFLQNNLELAISNTINNSPSDLSQEMILMPAEVFSPEATNVFSESYHLLLLDAQGNVLGGTFPS
RTPAGQPWNDAIVGNDRRVVQAALAGSDDIDQLTWKYENYLVIATPVRDQANQVIGALYVRSRPLSQNQVIVALLMFVIF
IASSIVSIIANALIGMVYGWFVARNFVRRLVHLTQATDSLAAGDLSVRVNDGSIDEIGHLARRFDSMAQQLESNVKMLRQ
LADRNAALVEQAGQLAIVEERNRLARDLHDSVSQELFSVTMLAAAARNLLPAQPDKARSQVEQLSQMAQRALHETRGLIF
ALRPAALGDQGLVPALRQLTEEAARRQGLQIELNTNGERRIPLDHEQALYRICQEALANVTKHSGVNSASVSLEYEAHRT
TLEVRDRGRGFDQDKPRNSHSLGLISIQERAKAVGGTVELTAAPGQGTSLRIVVPRTQTGLLVEPR
>Mature_546_residues
MQQHRKLTLVQRFGQRFGTLRWKLTWSYVWISVALTLLINGLAFGLVMGAAPPLNAPALLSAAQNHAAEFSEVLQEPTDQ
HTIQQLLRYRFGSLRGQFLQNNLELAISNTINNSPSDLSQEMILMPAEVFSPEATNVFSESYHLLLLDAQGNVLGGTFPS
RTPAGQPWNDAIVGNDRRVVQAALAGSDDIDQLTWKYENYLVIATPVRDQANQVIGALYVRSRPLSQNQVIVALLMFVIF
IASSIVSIIANALIGMVYGWFVARNFVRRLVHLTQATDSLAAGDLSVRVNDGSIDEIGHLARRFDSMAQQLESNVKMLRQ
LADRNAALVEQAGQLAIVEERNRLARDLHDSVSQELFSVTMLAAAARNLLPAQPDKARSQVEQLSQMAQRALHETRGLIF
ALRPAALGDQGLVPALRQLTEEAARRQGLQIELNTNGERRIPLDHEQALYRICQEALANVTKHSGVNSASVSLEYEAHRT
TLEVRDRGRGFDQDKPRNSHSLGLISIQERAKAVGGTVELTAAPGQGTSLRIVVPRTQTGLLVEPR

Specific function: Member of the two-component regulatory system liaS/liaR probably involved in response to a subset of cell wall-active antibiotics that interfere with the lipid II cycle in the cytoplasmic membrane (bacitracin, nisin, ramoplanin and vancomycin). Seems also

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 histidine kinase domain [H]

Homologues:

Organism=Escherichia coli, GI1787474, Length=227, Percent_Identity=27.7533039647577, Blast_Score=80, Evalue=3e-16,
Organism=Escherichia coli, GI1788812, Length=205, Percent_Identity=28.780487804878, Blast_Score=66, Evalue=6e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR003660
- InterPro:   IPR011712
- InterPro:   IPR005467
- InterPro:   IPR017202 [H]

Pfam domain/function: PF00672 HAMP; PF02518 HATPase_c; PF07730 HisKA_3 [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 60239; Mature: 60239

Theoretical pI: Translated: 7.92; Mature: 7.92

Prosite motif: PS50885 HAMP ; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQQHRKLTLVQRFGQRFGTLRWKLTWSYVWISVALTLLINGLAFGLVMGAAPPLNAPALL
CCCCHHHHHHHHHHHHHCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHH
SAAQNHAAEFSEVLQEPTDQHTIQQLLRYRFGSLRGQFLQNNLELAISNTINNSPSDLSQ
HHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCHHHHHH
EMILMPAEVFSPEATNVFSESYHLLLLDAQGNVLGGTFPSRTPAGQPWNDAIVGNDRRVV
HHHCCCHHHCCCCHHHHHCCCEEEEEEECCCCEECCCCCCCCCCCCCCCCCCCCCCHHHH
QAALAGSDDIDQLTWKYENYLVIATPVRDQANQVIGALYVRSRPLSQNQVIVALLMFVIF
HHHHCCCCCHHHHHEEECCEEEEEECCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHH
IASSIVSIIANALIGMVYGWFVARNFVRRLVHLTQATDSLAAGDLSVRVNDGSIDEIGHL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHH
ARRFDSMAQQLESNVKMLRQLADRNAALVEQAGQLAIVEERNRLARDLHDSVSQELFSVT
HHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCEEEHHHHHHHHHHHHHHHHHHHHHHH
MLAAAARNLLPAQPDKARSQVEQLSQMAQRALHETRGLIFALRPAALGDQGLVPALRQLT
HHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCCCCCHHHHHHHHH
EEAARRQGLQIELNTNGERRIPLDHEQALYRICQEALANVTKHSGVNSASVSLEYEAHRT
HHHHHHCCCEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEHHHHH
TLEVRDRGRGFDQDKPRNSHSLGLISIQERAKAVGGTVELTAAPGQGTSLRIVVPRTQTG
EEEEHHCCCCCCCCCCCCCCCEEEEEHHHHHHHCCCEEEEEECCCCCCEEEEEEECCCCC
LLVEPR
CEECCC
>Mature Secondary Structure
MQQHRKLTLVQRFGQRFGTLRWKLTWSYVWISVALTLLINGLAFGLVMGAAPPLNAPALL
CCCCHHHHHHHHHHHHHCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHH
SAAQNHAAEFSEVLQEPTDQHTIQQLLRYRFGSLRGQFLQNNLELAISNTINNSPSDLSQ
HHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCHHHHHH
EMILMPAEVFSPEATNVFSESYHLLLLDAQGNVLGGTFPSRTPAGQPWNDAIVGNDRRVV
HHHCCCHHHCCCCHHHHHCCCEEEEEEECCCCEECCCCCCCCCCCCCCCCCCCCCCHHHH
QAALAGSDDIDQLTWKYENYLVIATPVRDQANQVIGALYVRSRPLSQNQVIVALLMFVIF
HHHHCCCCCHHHHHEEECCEEEEEECCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHH
IASSIVSIIANALIGMVYGWFVARNFVRRLVHLTQATDSLAAGDLSVRVNDGSIDEIGHL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHH
ARRFDSMAQQLESNVKMLRQLADRNAALVEQAGQLAIVEERNRLARDLHDSVSQELFSVT
HHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCEEEHHHHHHHHHHHHHHHHHHHHHHH
MLAAAARNLLPAQPDKARSQVEQLSQMAQRALHETRGLIFALRPAALGDQGLVPALRQLT
HHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCCCCCHHHHHHHHH
EEAARRQGLQIELNTNGERRIPLDHEQALYRICQEALANVTKHSGVNSASVSLEYEAHRT
HHHHHHCCCEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEHHHHH
TLEVRDRGRGFDQDKPRNSHSLGLISIQERAKAVGGTVELTAAPGQGTSLRIVVPRTQTG
EEEEHHCCCCCCCCCCCCCCCEEEEEHHHHHHHCCCEEEEEECCCCCCEEEEEEECCCCC
LLVEPR
CEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9639930; 9384377 [H]