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The map label for this gene is hldE [H]

Identifier: 159897594

GI number: 159897594

Start: 1218059

End: 1218553

Strand: Reverse

Name: hldE [H]

Synonym: Haur_1065

Alternate gene names: 159897594

Gene position: 1218553-1218059 (Counterclockwise)

Preceding gene: 159897595

Following gene: 159897589

Centisome position: 19.2

GC content: 50.1

Gene sequence:

>495_bases
ATGAATTCACCATTTACTGATTTAGCCGATTTAGTGGCGCTCCGCCAGCAGTGGCGCGAACAGGGCTTGAGCGTGGTTTT
TACCAATGGCGCGTTCGATTTAGTCCATGCTGGCCATGTAACCTACTTGCAAGCAGCCCGAGCCTTGGGTGATCTGTTGA
TTGTTGGCTTGAATAGCGATGCGTCAGTCCGTGGCTACAAAGGCCCGACCCGCCCAATTGTGCCTGAGTTTCAGCGCGGA
ATTGTGCTAGCTGGTCTGCGTTCGGTCGATTATGTCACGCTATTTGAATCATTAACCGCTGAGCCATTAGTCAGTGCCTT
ACGACCTGATGTCTATGTCAAAGGCGGCGATTATGCTCACCCCGAGCAAGCCAGCAACGGCAAAGATTTACCTGAGGCGC
GTATTGTTTTAGCCTATGGCGGCAAAGTCGAACTAATTCAGTATCAAACAGGATTATCGACCAGCAATCTGATTGCCAAA
ATTCGCAGCGAATAA

Upstream 100 bases:

>100_bases
GCGGCGGCCTTGGTAGTACGACGAATTGGCGTGGCCACAGTTAGCCCCAACGAACTGCAAAACGCATTAATTCAATTTGG
CCAGATTGTATGAGGCTTCG

Downstream 100 bases:

>100_bases
AGCAACTGTTTGATTGAGAAAATGCTGTTTCTGTTTAAAACCACAAAGGACATGCAGCGCCGCATGTCCTCTGGCTAAAT
CATAGGCTCAATCCTATATT

Product: rfaE bifunctional protein

Products: diphosphate; CDPglycerol

Alternate protein names: D-beta-D-heptose 7-phosphate kinase; D-beta-D-heptose 7-phosphotransferase; D-beta-D-heptose 1-phosphate adenosyltransferase [H]

Number of amino acids: Translated: 164; Mature: 164

Protein sequence:

>164_residues
MNSPFTDLADLVALRQQWREQGLSVVFTNGAFDLVHAGHVTYLQAARALGDLLIVGLNSDASVRGYKGPTRPIVPEFQRG
IVLAGLRSVDYVTLFESLTAEPLVSALRPDVYVKGGDYAHPEQASNGKDLPEARIVLAYGGKVELIQYQTGLSTSNLIAK
IRSE

Sequences:

>Translated_164_residues
MNSPFTDLADLVALRQQWREQGLSVVFTNGAFDLVHAGHVTYLQAARALGDLLIVGLNSDASVRGYKGPTRPIVPEFQRG
IVLAGLRSVDYVTLFESLTAEPLVSALRPDVYVKGGDYAHPEQASNGKDLPEARIVLAYGGKVELIQYQTGLSTSNLIAK
IRSE
>Mature_164_residues
MNSPFTDLADLVALRQQWREQGLSVVFTNGAFDLVHAGHVTYLQAARALGDLLIVGLNSDASVRGYKGPTRPIVPEFQRG
IVLAGLRSVDYVTLFESLTAEPLVSALRPDVYVKGGDYAHPEQASNGKDLPEARIVLAYGGKVELIQYQTGLSTSNLIAK
IRSE

Specific function: Catalyzes the ADP transfer to D-glycero-D-manno-heptose 1-phosphate, yielding ADP-D,D-heptose [H]

COG id: COG2870

COG function: function code M; ADP-heptose synthase, bifunctional sugar kinase/adenylyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the cytidylyltransferase family [H]

Homologues:

Organism=Escherichia coli, GI1789432, Length=146, Percent_Identity=47.945205479452, Blast_Score=135, Evalue=1e-33,
Organism=Caenorhabditis elegans, GI193209647, Length=69, Percent_Identity=44.9275362318841, Blast_Score=65, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI115533408, Length=68, Percent_Identity=45.5882352941176, Blast_Score=65, Evalue=1e-11,
Organism=Drosophila melanogaster, GI45550975, Length=147, Percent_Identity=31.9727891156463, Blast_Score=70, Evalue=5e-13,
Organism=Drosophila melanogaster, GI45550974, Length=147, Percent_Identity=31.9727891156463, Blast_Score=70, Evalue=6e-13,
Organism=Drosophila melanogaster, GI45550973, Length=147, Percent_Identity=31.9727891156463, Blast_Score=70, Evalue=6e-13,
Organism=Drosophila melanogaster, GI19921220, Length=147, Percent_Identity=31.9727891156463, Blast_Score=70, Evalue=7e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR023030
- InterPro:   IPR011611
- InterPro:   IPR002173
- InterPro:   IPR004821
- InterPro:   IPR004820
- InterPro:   IPR011913
- InterPro:   IPR011914
- InterPro:   IPR014729 [H]

Pfam domain/function: PF01467 CTP_transf_2; PF00294 PfkB [H]

EC number: 2.7.7.39

Molecular weight: Translated: 17783; Mature: 17783

Theoretical pI: Translated: 6.26; Mature: 6.26

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
0.6 %Met     (Translated Protein)
0.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
0.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNSPFTDLADLVALRQQWREQGLSVVFTNGAFDLVHAGHVTYLQAARALGDLLIVGLNSD
CCCCHHHHHHHHHHHHHHHHCCCEEEEECCCEEEHCCCHHHHHHHHHHHCCEEEEEECCC
ASVRGYKGPTRPIVPEFQRGIVLAGLRSVDYVTLFESLTAEPLVSALRPDVYVKGGDYAH
CCCCCCCCCCCCCCCHHHCCEEEECCCCCHHHHHHHHHHHHHHHHHHCCCEEEECCCCCC
PEQASNGKDLPEARIVLAYGGKVELIQYQTGLSTSNLIAKIRSE
CCCCCCCCCCCCEEEEEEECCEEEEEEEECCCCHHHHHHHCCCC
>Mature Secondary Structure
MNSPFTDLADLVALRQQWREQGLSVVFTNGAFDLVHAGHVTYLQAARALGDLLIVGLNSD
CCCCHHHHHHHHHHHHHHHHCCCEEEEECCCEEEHCCCHHHHHHHHHHHCCEEEEEECCC
ASVRGYKGPTRPIVPEFQRGIVLAGLRSVDYVTLFESLTAEPLVSALRPDVYVKGGDYAH
CCCCCCCCCCCCCCCHHHCCEEEECCCCCHHHHHHHHHHHHHHHHHHCCCEEEECCCCCC
PEQASNGKDLPEARIVLAYGGKVELIQYQTGLSTSNLIAKIRSE
CCCCCCCCCCCCEEEEEEECCEEEEEEEECCCCHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: CTP; sn-glycerol 3-phosphate

Specific reaction: CTP + sn-glycerol 3-phosphate = diphosphate + CDP-glycerol

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA