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The map label for this gene is potB [H]

Identifier: 159897591

GI number: 159897591

Start: 1215012

End: 1215875

Strand: Direct

Name: potB [H]

Synonym: Haur_1062

Alternate gene names: 159897591

Gene position: 1215012-1215875 (Clockwise)

Preceding gene: 159897590

Following gene: 159897592

Centisome position: 19.14

GC content: 50.23

Gene sequence:

>864_bases
ATGTATAATGCACGAACTGAGCAACGCCGTGCCTGGTTGCAAGCCTCGCCTGCCCTGATTTGGCTGGGCTTCTTCTTTTT
GGCTCCCTTGGTGCTCATTTTAATTTATTCATTTCAAGGCCGCGCCGACGATGGCACGGTGCTCTGGAATTGGTCGCTGG
CGAACTATCAAAAGCTGTTTGCTGATGGTGCGTTTTGGCGCACGCTCTGGCTTTCGACTTGGATTGCGCTGGTGGTAACT
GCGATCACCTTGGTGGTCAGTTATCCCTTGGCCTTTTTTATGGCAACCCGCAGCCCTAAAATTCGTGGTGTGTTGGTGCT
TGCGGTCATGATTCCCTTTTGGACCAACTTTCTGGTGCGGATTTACGCCTGGAAAGTGCTGCTGGGCAATAATGGCGTGC
TCAATCGCTTTTTAGGCCAATTTGATCTGGGGCCGTTGCAAATTATCAATAGCCCTAGTGCGGTGATTATGGCGCTAATT
TATGGCGAGTTGCCGTATATGATTTTGCCTTTATATGCGGCGCTCGAACGTTTTGATTGGGTGCAACTTGAGGCGGCCCG
CGATTTAGGCGCTGGGCGATTTCAAACATTTTGGCGGGTGCTCTTGCCCCAAACCTTGCCAGGCATTACGGCTGGCTCAA
TTTTGGTGTTTGTGCCAACGGTTGGCACTTTCGCCGTCTCAGATATTTTGGGTGGCACAAGCGTGAATATGGTTGGTAAT
ACCTTGAGTTTGCAATTTGGTTCGGCCTCGAATTGGGCCTATGGCTCAGCGATTTCGATGATTTTTATGCTGTTACTCTT
GGGCGCAATCTTGCTCTATTTCAAAGCCACACGGGAGGTGAATGATGGCGAAAATCCTGGCTGA

Upstream 100 bases:

>100_bases
CATGGGCCACTCAAAATAGCCTCATTCTCGAACGTTAATTAATCGTACTCTGCTCAGGTTTGTTGGTTTGGTCAACCCAA
ACAACATCCATGGGGGAACT

Downstream 100 bases:

>100_bases
TAAGCGCATCGAAACCAAGCACGGGCGTTCGCGCCCAGTGGCCAAACGGCACATGCCTTGGGGGACATGGGCGCTAACGC
TGAATGCGATTTTCGTCTAC

Product: binding-protein-dependent transport systems inner membrane component

Products: phosphate; putrescine [Cytoplasm]; ADP; spermidine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 287; Mature: 287

Protein sequence:

>287_residues
MYNARTEQRRAWLQASPALIWLGFFFLAPLVLILIYSFQGRADDGTVLWNWSLANYQKLFADGAFWRTLWLSTWIALVVT
AITLVVSYPLAFFMATRSPKIRGVLVLAVMIPFWTNFLVRIYAWKVLLGNNGVLNRFLGQFDLGPLQIINSPSAVIMALI
YGELPYMILPLYAALERFDWVQLEAARDLGAGRFQTFWRVLLPQTLPGITAGSILVFVPTVGTFAVSDILGGTSVNMVGN
TLSLQFGSASNWAYGSAISMIFMLLLLGAILLYFKATREVNDGENPG

Sequences:

>Translated_287_residues
MYNARTEQRRAWLQASPALIWLGFFFLAPLVLILIYSFQGRADDGTVLWNWSLANYQKLFADGAFWRTLWLSTWIALVVT
AITLVVSYPLAFFMATRSPKIRGVLVLAVMIPFWTNFLVRIYAWKVLLGNNGVLNRFLGQFDLGPLQIINSPSAVIMALI
YGELPYMILPLYAALERFDWVQLEAARDLGAGRFQTFWRVLLPQTLPGITAGSILVFVPTVGTFAVSDILGGTSVNMVGN
TLSLQFGSASNWAYGSAISMIFMLLLLGAILLYFKATREVNDGENPG
>Mature_287_residues
MYNARTEQRRAWLQASPALIWLGFFFLAPLVLILIYSFQGRADDGTVLWNWSLANYQKLFADGAFWRTLWLSTWIALVVT
AITLVVSYPLAFFMATRSPKIRGVLVLAVMIPFWTNFLVRIYAWKVLLGNNGVLNRFLGQFDLGPLQIINSPSAVIMALI
YGELPYMILPLYAALERFDWVQLEAARDLGAGRFQTFWRVLLPQTLPGITAGSILVFVPTVGTFAVSDILGGTSVNMVGN
TLSLQFGSASNWAYGSAISMIFMLLLLGAILLYFKATREVNDGENPG

Specific function: Required for the activity of the bacterial periplasmic transport system of putrescine and spermidine [H]

COG id: COG1176

COG function: function code E; ABC-type spermidine/putrescine transport system, permease component I

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1787369, Length=265, Percent_Identity=40.7547169811321, Blast_Score=201, Evalue=3e-53,
Organism=Escherichia coli, GI1787080, Length=245, Percent_Identity=40, Blast_Score=174, Evalue=8e-45,
Organism=Escherichia coli, GI1787713, Length=266, Percent_Identity=33.0827067669173, Blast_Score=115, Evalue=4e-27,
Organism=Escherichia coli, GI1787570, Length=236, Percent_Identity=25.8474576271186, Blast_Score=67, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 31994; Mature: 31994

Theoretical pI: Translated: 9.68; Mature: 9.68

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYNARTEQRRAWLQASPALIWLGFFFLAPLVLILIYSFQGRADDGTVLWNWSLANYQKLF
CCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECHHHHHHHH
ADGAFWRTLWLSTWIALVVTAITLVVSYPLAFFMATRSPKIRGVLVLAVMIPFWTNFLVR
HCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHH
IYAWKVLLGNNGVLNRFLGQFDLGPLQIINSPSAVIMALIYGELPYMILPLYAALERFDW
HHHHHHHCCCCCHHHHHHCCCCCCHHEEECCCHHHHHHHHHHCCHHHHHHHHHHHHHCCH
VQLEAARDLGAGRFQTFWRVLLPQTLPGITAGSILVFVPTVGTFAVSDILGGTSVNMVGN
HHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCCEEEEECCCHHHHHHHHHCCCEEEECCC
TLSLQFGSASNWAYGSAISMIFMLLLLGAILLYFKATREVNDGENPG
EEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure
MYNARTEQRRAWLQASPALIWLGFFFLAPLVLILIYSFQGRADDGTVLWNWSLANYQKLF
CCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECHHHHHHHH
ADGAFWRTLWLSTWIALVVTAITLVVSYPLAFFMATRSPKIRGVLVLAVMIPFWTNFLVR
HCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHH
IYAWKVLLGNNGVLNRFLGQFDLGPLQIINSPSAVIMALIYGELPYMILPLYAALERFDW
HHHHHHHCCCCCHHHHHHCCCCCCHHEEECCCHHHHHHHHHHCCHHHHHHHHHHHHHCCH
VQLEAARDLGAGRFQTFWRVLLPQTLPGITAGSILVFVPTVGTFAVSDILGGTSVNMVGN
HHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCCEEEEECCCHHHHHHHHHCCCEEEECCC
TLSLQFGSASNWAYGSAISMIFMLLLLGAILLYFKATREVNDGENPG
EEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: putrescine [Periplasm]; H2O; ATP; spermidine [Periplasm] [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] ATP + spermidine [Periplasm] + H2O = ADP + phosphate + spermidine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11677608; 12644504 [H]