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The map label for this gene is ltaA [H]

Identifier: 159897565

GI number: 159897565

Start: 1182413

End: 1183447

Strand: Direct

Name: ltaA [H]

Synonym: Haur_1036

Alternate gene names: 159897565

Gene position: 1182413-1183447 (Clockwise)

Preceding gene: 159897555

Following gene: 159897566

Centisome position: 18.63

GC content: 53.24

Gene sequence:

>1035_bases
ATGATCGATTTGCGCAGCGATACCGTTACGAAGCCAAGTTTGGCCATGCGTGAGGCCATGCATCATGCCGAGGTTGGTGA
CGATGTGTTTGGTGATGATCCAACGGTCAATCAATTACAGCGCTATGCCGCCGATCTGGTTGGCAAAGAGGCGGCGATTT
TTGTGCCAAGCGGCACGATGGGCAATTTGGCGGCAATTTTGGCCCATGCTGGGCGTGGTCAAGAACTGTTGCTCGGTGAT
GAATCGCATATTTATCATTATGAGGCGGGTGGCGCTTCAGCCTTGGGTGGCTTGGTGTTTCATCCCATCCCAACCAATGC
CCAAGGCGAGCTGGATTTAGCGGCTTTGAATGCGGCAGTACGCCCAGCCTACGATGCCCATGCGGCTCAGGCAGGCCTGG
TGTGTCTAGAAAATAGTCACAATCGCTGTGGTGGCACAGTGCTTTCGTTAGAATATTTGGCGCAAGTACAGCAATGGGCC
AGCAGCCAAAACTTGCCAGTGCATATGGATGGCGCTCGGGTGTTCAATGCAGCGGTGGCGCTCGGCGTGCCAGCTAGCAC
CATCACCAAGCATGTCGATAGCGTGCAATTTTGCTTATCCAAAGGCTTGGGTGCACCAATTGGCTCGATTGTGGCTGGCT
CAGGCGAGTTTATCAAAAAGGTGCATCGCTGGCGCAAAATGCTTGGCGGCGGCATGCGCCAAGTTGGCGTAGTCGCAGCG
GCGGGCATGATTGCGCTCAACGAAGGCCGCGAACGCTTGATCGATGACCATGTAAATGCCAAAATGCTGGCCGAAGCACT
CAGTCAATTGCCTCAAATTGAGCTTGATTTGGCTTCGGTGCAAACCAATATTATCGTTTTTGGCTTGCGTGATAGCACGT
TTAGCCCTGAACAATTGGTTGAACGTTTACGCCAAGCAGGGGTTTTGATCGTGCCATTCAAAGGCCGTTTACGGGCTGTA
ACCCATGTTGATGTTAATCGTGAGCAATGCACCGAGGCCTCCAGCATCATCGCCGAGGTGCTGCAAAGCGCTTAA

Upstream 100 bases:

>100_bases
TTTGTTCCCGAAAGCTTTTGATATAATTGTTTGGTGCTTCAGTAGCCACTTGTCAGCAACTTTAGGTGGAAAATTGGCTA
AAATTTTGAAGGAGTTAACC

Downstream 100 bases:

>100_bases
TCCCGTTACAAATTTTGCACTTTCGCGCCCTGCGGCTCGTGTATCCTATAGACATCAATAATCGCATCTAGACAAGGATT
TTGGGCTATGACTGAAATAG

Product: threonine aldolase

Products: glycine; acetaldehyde

Alternate protein names: L-allo-TA; L-allo-threonine acetaldehyde-lyase [H]

Number of amino acids: Translated: 344; Mature: 344

Protein sequence:

>344_residues
MIDLRSDTVTKPSLAMREAMHHAEVGDDVFGDDPTVNQLQRYAADLVGKEAAIFVPSGTMGNLAAILAHAGRGQELLLGD
ESHIYHYEAGGASALGGLVFHPIPTNAQGELDLAALNAAVRPAYDAHAAQAGLVCLENSHNRCGGTVLSLEYLAQVQQWA
SSQNLPVHMDGARVFNAAVALGVPASTITKHVDSVQFCLSKGLGAPIGSIVAGSGEFIKKVHRWRKMLGGGMRQVGVVAA
AGMIALNEGRERLIDDHVNAKMLAEALSQLPQIELDLASVQTNIIVFGLRDSTFSPEQLVERLRQAGVLIVPFKGRLRAV
THVDVNREQCTEASSIIAEVLQSA

Sequences:

>Translated_344_residues
MIDLRSDTVTKPSLAMREAMHHAEVGDDVFGDDPTVNQLQRYAADLVGKEAAIFVPSGTMGNLAAILAHAGRGQELLLGD
ESHIYHYEAGGASALGGLVFHPIPTNAQGELDLAALNAAVRPAYDAHAAQAGLVCLENSHNRCGGTVLSLEYLAQVQQWA
SSQNLPVHMDGARVFNAAVALGVPASTITKHVDSVQFCLSKGLGAPIGSIVAGSGEFIKKVHRWRKMLGGGMRQVGVVAA
AGMIALNEGRERLIDDHVNAKMLAEALSQLPQIELDLASVQTNIIVFGLRDSTFSPEQLVERLRQAGVLIVPFKGRLRAV
THVDVNREQCTEASSIIAEVLQSA
>Mature_344_residues
MIDLRSDTVTKPSLAMREAMHHAEVGDDVFGDDPTVNQLQRYAADLVGKEAAIFVPSGTMGNLAAILAHAGRGQELLLGD
ESHIYHYEAGGASALGGLVFHPIPTNAQGELDLAALNAAVRPAYDAHAAQAGLVCLENSHNRCGGTVLSLEYLAQVQQWA
SSQNLPVHMDGARVFNAAVALGVPASTITKHVDSVQFCLSKGLGAPIGSIVAGSGEFIKKVHRWRKMLGGGMRQVGVVAA
AGMIALNEGRERLIDDHVNAKMLAEALSQLPQIELDLASVQTNIIVFGLRDSTFSPEQLVERLRQAGVLIVPFKGRLRAV
THVDVNREQCTEASSIIAEVLQSA

Specific function: Stereospecifically catalyzes the interconversion of L- allo-threonine and glycine [H]

COG id: COG2008

COG function: function code E; Threonine aldolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the threonine aldolase family [H]

Homologues:

Organism=Escherichia coli, GI1787095, Length=337, Percent_Identity=48.6646884272997, Blast_Score=297, Evalue=6e-82,
Organism=Caenorhabditis elegans, GI193206522, Length=347, Percent_Identity=40.6340057636888, Blast_Score=247, Evalue=5e-66,
Organism=Caenorhabditis elegans, GI193206524, Length=347, Percent_Identity=40.6340057636888, Blast_Score=247, Evalue=6e-66,
Organism=Caenorhabditis elegans, GI193206520, Length=347, Percent_Identity=40.6340057636888, Blast_Score=247, Evalue=7e-66,
Organism=Saccharomyces cerevisiae, GI6320789, Length=345, Percent_Identity=33.9130434782609, Blast_Score=176, Evalue=3e-45,
Organism=Drosophila melanogaster, GI21355439, Length=313, Percent_Identity=47.2843450479233, Blast_Score=265, Evalue=4e-71,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001597
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF01212 Beta_elim_lyase [H]

EC number: 4.1.2.5

Molecular weight: Translated: 36543; Mature: 36543

Theoretical pI: Translated: 6.18; Mature: 6.18

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIDLRSDTVTKPSLAMREAMHHAEVGDDVFGDDPTVNQLQRYAADLVGKEAAIFVPSGTM
CCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHCCCEEEEECCCCC
GNLAAILAHAGRGQELLLGDESHIYHYEAGGASALGGLVFHPIPTNAQGELDLAALNAAV
HHHHHHHHHCCCCCEEEECCCCCEEEEECCCCHHHCCEEEECCCCCCCCCCHHHHHHHHH
RPAYDAHAAQAGLVCLENSHNRCGGTVLSLEYLAQVQQWASSQNLPVHMDGARVFNAAVA
CCCHHHHHHHHCEEEEECCCCCCCCHHHHHHHHHHHHHHHCCCCCCEEECCHHHHHHHHH
LGVPASTITKHVDSVQFCLSKGLGAPIGSIVAGSGEFIKKVHRWRKMLGGGMRQVGVVAA
HCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHCCCHHHHHHHHHHHHHHCCCHHHHHHHHH
AGMIALNEGRERLIDDHVNAKMLAEALSQLPQIELDLASVQTNIIVFGLRDSTFSPEQLV
HHHHEECCCHHHHHHHCCCHHHHHHHHHHCCCEEEEHHHEEEEEEEEEECCCCCCHHHHH
ERLRQAGVLIVPFKGRLRAVTHVDVNREQCTEASSIIAEVLQSA
HHHHHCCEEEEECCCCEEEEEEECCCHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MIDLRSDTVTKPSLAMREAMHHAEVGDDVFGDDPTVNQLQRYAADLVGKEAAIFVPSGTM
CCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHCCCEEEEECCCCC
GNLAAILAHAGRGQELLLGDESHIYHYEAGGASALGGLVFHPIPTNAQGELDLAALNAAV
HHHHHHHHHCCCCCEEEECCCCCEEEEECCCCHHHCCEEEECCCCCCCCCCHHHHHHHHH
RPAYDAHAAQAGLVCLENSHNRCGGTVLSLEYLAQVQQWASSQNLPVHMDGARVFNAAVA
CCCHHHHHHHHCEEEEECCCCCCCCHHHHHHHHHHHHHHHCCCCCCEEECCHHHHHHHHH
LGVPASTITKHVDSVQFCLSKGLGAPIGSIVAGSGEFIKKVHRWRKMLGGGMRQVGVVAA
HCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHCCCHHHHHHHHHHHHHHCCCHHHHHHHHH
AGMIALNEGRERLIDDHVNAKMLAEALSQLPQIELDLASVQTNIIVFGLRDSTFSPEQLV
HHHHEECCCHHHHHHHCCCHHHHHHHHHHCCCEEEEHHHEEEEEEEEEECCCCCCHHHHH
ERLRQAGVLIVPFKGRLRAVTHVDVNREQCTEASSIIAEVLQSA
HHHHHCCEEEEECCCCEEEEEEECCCHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: Pyridoxal phosphate [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: 1.7

Km value (mM): 2.85 {L-Thr}} 0.12 {DL-threo-phenylserine}} 0.24 {DL-erythro-phenylserine}} 0.22 {L-allo-Thr}} [C]

Substrates: L-threonine

Specific reaction: L-threonine = glycine + acetaldehyde

General reaction: Elimination of an aldehyde, C-C bond cleavage [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9171400; 9228760 [H]