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The map label for this gene is mls [H]

Identifier: 159897545

GI number: 159897545

Start: 1161510

End: 1163102

Strand: Direct

Name: mls [H]

Synonym: Haur_1016

Alternate gene names: 159897545

Gene position: 1161510-1163102 (Clockwise)

Preceding gene: 159897544

Following gene: 159897546

Centisome position: 18.3

GC content: 51.29

Gene sequence:

>1593_bases
ATGACCGATCGGCAACATGGCGTGAAAATCAACGCCCCTATCACCCCCGCTGCGGCGGAATTGTTGACGGAACCAGCCCT
ACACTTCTTAGCTGCCTTGCATCGCACTTTTGACCAAACTCGCCGCGACTTATTGCTCGGACGAGTGGAACGCCAAAGCC
GCCTTGATGCAGGCGAAAACCCTGATTTTCTCGCTGAAACTGCCCATATTCGTGCTAGCGACTGGCAAATTGCGCCCATC
CCCGATGAGATTCGTAATCGTCGCGTGGAAATTACTGGGCCAATTGATCGCAAAATGATCATCAATGCGCTCAACTCTGG
AGCCAATGTCTTCATGGCCGACTGTGAAGATGCAACCACTCCAAGCTGGGATAATTTGGTCAGCGGCCAACTTAACTTGC
GCGATGCGGTCAATCGGACGATAAGCTTCACCAATGAAGCTGGCAAAGCCTATCAATTAAACGATCAGGTTGCGGTGCTG
TTTGTGCGGCCTCGTGGCTGGCACTTGCTCGAAAAGCATGTCACCGTCGATGGCGAACCCTTGGCTGGTGGTCTGTTCGA
CTTTGGTTTGTATTTGTTCCACAATGCCAAAACCTTGCTCGAACGTGGCTCGGCTCCTTACTTCTATCTGCCAAAACTCG
AAAGCCATCGCGAAGCCCGTTTGTGGAATGATGTGTTCGTGTTTGCCCAAAAGCAACTCGGCCTGCCCCATGGCTCAATC
AAGGCAACGGTTTTGATTGAAACAATTTTGGCCGCCTTCGAGATGGACGAAATTCTGTATGAATTGCGCGACCACTCGGC
TGGCCTCAACTGTGGCCGCTGGGATTACATCTTCAGCTGCATCAAGAAATTTGCTAAATTACAACATTTTGTGCTGGCTG
ATCGTGCTTTAGTGACGATGACTTCACGCTTTATGCGCTCATATTCGTTGCTGGCGATCAAAACCTGCCATCGCCGTGGT
GCTCACGCAATGGGCGGGATGGCTGCTCAGATTCCGATCAAGCACGATGCCCAAGCCAATGCCGAAGCCCTCGCCAAAGT
GCAAGCCGATAAAGAGCGCGAAGCTCGCGACGGCCACGACGGCACATGGGTCGCTCATCCAGGTTTGGTTCCGTTAGCTA
AGGCCGCCTTTGATGCTTTGATGCCTGAAGCTAACCAAATTGGCAAGCAGCTTGATGTTGAAATTACTGCCGATGATTTA
CTGCGCTTCGAGCCATCAGCGCCGATTACCGAGCAAGGCCTGCGCAAAAATATCAGCGTTGGCATCCAATATATCGAAGC
TTGGTTGGGTGGCTTAGGCTGCGTGCCGCTGTACAACTTAATGGAAGATGCCGCAACCGCCGAAATCTCCCGTGCTCAAG
TTTGGCAATGGGTACATCAACCTAATGGCATTACCGAAGATTTTCGCAAAATCACCCTCGATTGGGTGCGCGAGTTGATC
GTCGAAGAACTGGCCAAGATCGAACAAGAAGTTGGCGCAGAACGCTATCGCAACGGTCATTATGATCGGGCTAGCCAATT
GTTTGATCAATTGGTTGCCAACCCAACCTTTACCGAATTTCTCACGCTTCCTGCTTACGAACAAATCGATTAA

Upstream 100 bases:

>100_bases
AAAATTCGCTGGCGATAAAAATTATCTACCAAGCCAATTTTTACCAGAATCCATGCACCAATCCTACGTCGATGGTGACG
CACCGGAAAGGAACGACCGC

Downstream 100 bases:

>100_bases
TGTGAATTAATTGGCAGCTTGCAGGTGCTGGCATCAAACAACAATGACCAAAACCTGCAATTCTACGCTGGGATAGATTC
TTGATTTTCATTACCTTGGC

Product: malate synthase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 530; Mature: 529

Protein sequence:

>530_residues
MTDRQHGVKINAPITPAAAELLTEPALHFLAALHRTFDQTRRDLLLGRVERQSRLDAGENPDFLAETAHIRASDWQIAPI
PDEIRNRRVEITGPIDRKMIINALNSGANVFMADCEDATTPSWDNLVSGQLNLRDAVNRTISFTNEAGKAYQLNDQVAVL
FVRPRGWHLLEKHVTVDGEPLAGGLFDFGLYLFHNAKTLLERGSAPYFYLPKLESHREARLWNDVFVFAQKQLGLPHGSI
KATVLIETILAAFEMDEILYELRDHSAGLNCGRWDYIFSCIKKFAKLQHFVLADRALVTMTSRFMRSYSLLAIKTCHRRG
AHAMGGMAAQIPIKHDAQANAEALAKVQADKEREARDGHDGTWVAHPGLVPLAKAAFDALMPEANQIGKQLDVEITADDL
LRFEPSAPITEQGLRKNISVGIQYIEAWLGGLGCVPLYNLMEDAATAEISRAQVWQWVHQPNGITEDFRKITLDWVRELI
VEELAKIEQEVGAERYRNGHYDRASQLFDQLVANPTFTEFLTLPAYEQID

Sequences:

>Translated_530_residues
MTDRQHGVKINAPITPAAAELLTEPALHFLAALHRTFDQTRRDLLLGRVERQSRLDAGENPDFLAETAHIRASDWQIAPI
PDEIRNRRVEITGPIDRKMIINALNSGANVFMADCEDATTPSWDNLVSGQLNLRDAVNRTISFTNEAGKAYQLNDQVAVL
FVRPRGWHLLEKHVTVDGEPLAGGLFDFGLYLFHNAKTLLERGSAPYFYLPKLESHREARLWNDVFVFAQKQLGLPHGSI
KATVLIETILAAFEMDEILYELRDHSAGLNCGRWDYIFSCIKKFAKLQHFVLADRALVTMTSRFMRSYSLLAIKTCHRRG
AHAMGGMAAQIPIKHDAQANAEALAKVQADKEREARDGHDGTWVAHPGLVPLAKAAFDALMPEANQIGKQLDVEITADDL
LRFEPSAPITEQGLRKNISVGIQYIEAWLGGLGCVPLYNLMEDAATAEISRAQVWQWVHQPNGITEDFRKITLDWVRELI
VEELAKIEQEVGAERYRNGHYDRASQLFDQLVANPTFTEFLTLPAYEQID
>Mature_529_residues
TDRQHGVKINAPITPAAAELLTEPALHFLAALHRTFDQTRRDLLLGRVERQSRLDAGENPDFLAETAHIRASDWQIAPIP
DEIRNRRVEITGPIDRKMIINALNSGANVFMADCEDATTPSWDNLVSGQLNLRDAVNRTISFTNEAGKAYQLNDQVAVLF
VRPRGWHLLEKHVTVDGEPLAGGLFDFGLYLFHNAKTLLERGSAPYFYLPKLESHREARLWNDVFVFAQKQLGLPHGSIK
ATVLIETILAAFEMDEILYELRDHSAGLNCGRWDYIFSCIKKFAKLQHFVLADRALVTMTSRFMRSYSLLAIKTCHRRGA
HAMGGMAAQIPIKHDAQANAEALAKVQADKEREARDGHDGTWVAHPGLVPLAKAAFDALMPEANQIGKQLDVEITADDLL
RFEPSAPITEQGLRKNISVGIQYIEAWLGGLGCVPLYNLMEDAATAEISRAQVWQWVHQPNGITEDFRKITLDWVRELIV
EELAKIEQEVGAERYRNGHYDRASQLFDQLVANPTFTEFLTLPAYEQID

Specific function: Glyoxylate bypass; second step. [C]

COG id: COG2225

COG function: function code C; Malate synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the malate synthase family [H]

Homologues:

Organism=Escherichia coli, GI1790444, Length=521, Percent_Identity=52.5911708253359, Blast_Score=542, Evalue=1e-155,
Organism=Caenorhabditis elegans, GI17561814, Length=513, Percent_Identity=53.6062378167641, Blast_Score=536, Evalue=1e-152,
Organism=Caenorhabditis elegans, GI71982926, Length=406, Percent_Identity=56.4039408866995, Blast_Score=454, Evalue=1e-128,
Organism=Saccharomyces cerevisiae, GI6322222, Length=524, Percent_Identity=49.618320610687, Blast_Score=515, Evalue=1e-147,
Organism=Saccharomyces cerevisiae, GI6324212, Length=523, Percent_Identity=48.1835564053537, Blast_Score=501, Evalue=1e-142,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011076
- InterPro:   IPR006252
- InterPro:   IPR001465
- InterPro:   IPR019830 [H]

Pfam domain/function: PF01274 Malate_synthase [H]

EC number: =2.3.3.9 [H]

Molecular weight: Translated: 59592; Mature: 59460

Theoretical pI: Translated: 5.93; Mature: 5.93

Prosite motif: PS00510 MALATE_SYNTHASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDRQHGVKINAPITPAAAELLTEPALHFLAALHRTFDQTRRDLLLGRVERQSRLDAGEN
CCCCCCCEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
PDFLAETAHIRASDWQIAPIPDEIRNRRVEITGPIDRKMIINALNSGANVFMADCEDATT
CCHHHHHHEEEECCCEECCCCHHHCCCEEEEECCCHHHHHHHHHCCCCCEEEECCCCCCC
PSWDNLVSGQLNLRDAVNRTISFTNEAGKAYQLNDQVAVLFVRPRGWHLLEKHVTVDGEP
CCHHHHCCCCCCHHHHHHHHEEECCCCCCEEEECCCEEEEEECCCCCEEHHHCCCCCCCC
LAGGLFDFGLYLFHNAKTLLERGSAPYFYLPKLESHREARLWNDVFVFAQKQLGLPHGSI
CCCHHHHHHHHHHHHHHHHHHCCCCCEEECCCCCCCHHHHHHHHHHHHHHHHHCCCCCCH
KATVLIETILAAFEMDEILYELRDHSAGLNCGRWDYIFSCIKKFAKLQHFVLADRALVTM
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TSRFMRSYSLLAIKTCHRRGAHAMGGMAAQIPIKHDAQANAEALAKVQADKEREARDGHD
HHHHHHHHHHHHHHHHHHCCCHHHCCEEEECCCCCCCCCCHHHHHHHHHHHHHHHCCCCC
GTWVAHPGLVPLAKAAFDALMPEANQIGKQLDVEITADDLLRFEPSAPITEQGLRKNISV
CCEEECCCCHHHHHHHHHHHCCCHHHCCCCEEEEEEHHHHHEECCCCCCHHHHHHHHHHH
GIQYIEAWLGGLGCVPLYNLMEDAATAEISRAQVWQWVHQPNGITEDFRKITLDWVRELI
HHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHH
VEELAKIEQEVGAERYRNGHYDRASQLFDQLVANPTFTEFLTLPAYEQID
HHHHHHHHHHHCHHHHCCCCCHHHHHHHHHHHCCCCHHHHEECCCHHCCC
>Mature Secondary Structure 
TDRQHGVKINAPITPAAAELLTEPALHFLAALHRTFDQTRRDLLLGRVERQSRLDAGEN
CCCCCCEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
PDFLAETAHIRASDWQIAPIPDEIRNRRVEITGPIDRKMIINALNSGANVFMADCEDATT
CCHHHHHHEEEECCCEECCCCHHHCCCEEEEECCCHHHHHHHHHCCCCCEEEECCCCCCC
PSWDNLVSGQLNLRDAVNRTISFTNEAGKAYQLNDQVAVLFVRPRGWHLLEKHVTVDGEP
CCHHHHCCCCCCHHHHHHHHEEECCCCCCEEEECCCEEEEEECCCCCEEHHHCCCCCCCC
LAGGLFDFGLYLFHNAKTLLERGSAPYFYLPKLESHREARLWNDVFVFAQKQLGLPHGSI
CCCHHHHHHHHHHHHHHHHHHCCCCCEEECCCCCCCHHHHHHHHHHHHHHHHHCCCCCCH
KATVLIETILAAFEMDEILYELRDHSAGLNCGRWDYIFSCIKKFAKLQHFVLADRALVTM
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TSRFMRSYSLLAIKTCHRRGAHAMGGMAAQIPIKHDAQANAEALAKVQADKEREARDGHD
HHHHHHHHHHHHHHHHHHCCCHHHCCEEEECCCCCCCCCCHHHHHHHHHHHHHHHCCCCC
GTWVAHPGLVPLAKAAFDALMPEANQIGKQLDVEITADDLLRFEPSAPITEQGLRKNISV
CCEEECCCCHHHHHHHHHHHCCCHHHCCCCEEEEEEHHHHHEECCCCCCHHHHHHHHHHH
GIQYIEAWLGGLGCVPLYNLMEDAATAEISRAQVWQWVHQPNGITEDFRKITLDWVRELI
HHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHH
VEELAKIEQEVGAERYRNGHYDRASQLFDQLVANPTFTEFLTLPAYEQID
HHHHHHHHHHHCHHHHCCCCCHHHHHHHHHHHCCCCHHHHEECCCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA