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The map label for this gene is cobB1 [H]

Identifier: 159897216

GI number: 159897216

Start: 790847

End: 791578

Strand: Direct

Name: cobB1 [H]

Synonym: Haur_0687

Alternate gene names: 159897216

Gene position: 790847-791578 (Clockwise)

Preceding gene: 159897211

Following gene: 159897218

Centisome position: 12.46

GC content: 51.09

Gene sequence:

>732_bases
ATGGCCGAATTTGCTGCTGACTTAATTCAAACCTTACGCCAAACCCAATCGTTGGTGGTGTTAACAGGCTCGGGAATTTC
GGCTCCCAGCGGAATCCCGACCTATCGTAGTGCTGCCGCCGATGCCCGTTGGACGGCCTATGATCCTGATAAAGTTGCGA
CCTTCGCGGGCTTTGAGCGTGATCCGGTTGGAGTTTTTCAGGTGTATCAAGCCATGAAACGCCAGTGTGAGGCAGCTCAA
CCAAATGCTGGGCATTATGCCTTGGCGCAGTTGGAGCAATTGGGTACACAATTTAAGCTGTTTACCCAAAATATTGATAG
TTTGCATCAACGAGCTGGCTCAAGCCAGGTTTACGAGGTGCATGGCAGTTTGGCTCGCACGATCTGTTCGCGTGAGGGCA
ACTTGGTTGAAACCTGGAATGCTGAGCAGCCAATTTGCCCGGCTTGTGGTGCGCCGCTGCGGCCTGATATTGTTTGGTTT
GGTGAATTGTTGGATGCTGGAATTTTGCAAGCGGCCAAAGCGGCGTTTGATAGCAGCGATGTGGCTTTGGTGATTGGCAC
TTCGGCGATTGTTGAGCCAATTGCTTCGTTGCCGCATCGAGCCTTGCGGCGCAAAAAAACCGTAATTGAAATTAATCCTG
ATATCCCGTTGCGAGGGATTGCGACGTTTTCACTGGCAGGCAGTGCCGATGAGCTGTTGCCACAATTGATTAAGGAGGTT
TGGAAGCTTTAA

Upstream 100 bases:

>100_bases
CGTTAATAAAATTTGATTATCCCAAGGCCAATAACTATTTGCACTAGCATTTGATAAATAGCACAATAGTTCTGATTATT
ATTGATAAGGAGATCAATTC

Downstream 100 bases:

>100_bases
ATGCCCTCACCCCAACCCCTCTCCCGCTGCGGCAGGCGAGGGGCATTGCTTAGGTTGTTAGCCTAAGCTGTTCTGCGCTT
TGTGTCCTTCGCGGTTTCGT

Product: silent information regulator protein Sir2

Products: NA

Alternate protein names: Regulatory protein SIR2 homolog 1; SIR2-Af1 [H]

Number of amino acids: Translated: 243; Mature: 242

Protein sequence:

>243_residues
MAEFAADLIQTLRQTQSLVVLTGSGISAPSGIPTYRSAAADARWTAYDPDKVATFAGFERDPVGVFQVYQAMKRQCEAAQ
PNAGHYALAQLEQLGTQFKLFTQNIDSLHQRAGSSQVYEVHGSLARTICSREGNLVETWNAEQPICPACGAPLRPDIVWF
GELLDAGILQAAKAAFDSSDVALVIGTSAIVEPIASLPHRALRRKKTVIEINPDIPLRGIATFSLAGSADELLPQLIKEV
WKL

Sequences:

>Translated_243_residues
MAEFAADLIQTLRQTQSLVVLTGSGISAPSGIPTYRSAAADARWTAYDPDKVATFAGFERDPVGVFQVYQAMKRQCEAAQ
PNAGHYALAQLEQLGTQFKLFTQNIDSLHQRAGSSQVYEVHGSLARTICSREGNLVETWNAEQPICPACGAPLRPDIVWF
GELLDAGILQAAKAAFDSSDVALVIGTSAIVEPIASLPHRALRRKKTVIEINPDIPLRGIATFSLAGSADELLPQLIKEV
WKL
>Mature_242_residues
AEFAADLIQTLRQTQSLVVLTGSGISAPSGIPTYRSAAADARWTAYDPDKVATFAGFERDPVGVFQVYQAMKRQCEAAQP
NAGHYALAQLEQLGTQFKLFTQNIDSLHQRAGSSQVYEVHGSLARTICSREGNLVETWNAEQPICPACGAPLRPDIVWFG
ELLDAGILQAAKAAFDSSDVALVIGTSAIVEPIASLPHRALRRKKTVIEINPDIPLRGIATFSLAGSADELLPQLIKEVW
KL

Specific function: Deacetylates the N-terminal lysine residue of Alba, the major archaeal chromatin protein and that, in turn, increases Alba's DNA binding affinity, thereby repressing transcription per subunit [H]

COG id: COG0846

COG function: function code K; NAD-dependent protein deacetylases, SIR2 family

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 deacetylase sirtuin-type domain [H]

Homologues:

Organism=Homo sapiens, GI300795542, Length=247, Percent_Identity=35.2226720647773, Blast_Score=149, Evalue=2e-36,
Organism=Homo sapiens, GI6912664, Length=265, Percent_Identity=32.8301886792453, Blast_Score=142, Evalue=4e-34,
Organism=Homo sapiens, GI13787215, Length=241, Percent_Identity=34.0248962655602, Blast_Score=139, Evalue=3e-33,
Organism=Homo sapiens, GI6912660, Length=224, Percent_Identity=34.8214285714286, Blast_Score=105, Evalue=3e-23,
Organism=Homo sapiens, GI6912662, Length=281, Percent_Identity=29.8932384341637, Blast_Score=103, Evalue=1e-22,
Organism=Homo sapiens, GI63054862, Length=209, Percent_Identity=34.4497607655502, Blast_Score=100, Evalue=2e-21,
Organism=Homo sapiens, GI7657575, Length=215, Percent_Identity=33.4883720930233, Blast_Score=98, Evalue=8e-21,
Organism=Homo sapiens, GI13775602, Length=217, Percent_Identity=30.4147465437788, Blast_Score=91, Evalue=8e-19,
Organism=Homo sapiens, GI13775600, Length=217, Percent_Identity=30.4147465437788, Blast_Score=90, Evalue=2e-18,
Organism=Homo sapiens, GI300797705, Length=193, Percent_Identity=29.0155440414508, Blast_Score=83, Evalue=2e-16,
Organism=Homo sapiens, GI215982798, Length=158, Percent_Identity=32.2784810126582, Blast_Score=74, Evalue=1e-13,
Organism=Escherichia coli, GI308199517, Length=235, Percent_Identity=39.1489361702128, Blast_Score=145, Evalue=2e-36,
Organism=Caenorhabditis elegans, GI17541892, Length=257, Percent_Identity=29.9610894941634, Blast_Score=104, Evalue=4e-23,
Organism=Caenorhabditis elegans, GI71990482, Length=282, Percent_Identity=25.886524822695, Blast_Score=85, Evalue=3e-17,
Organism=Saccharomyces cerevisiae, GI6325242, Length=222, Percent_Identity=28.8288288288288, Blast_Score=79, Evalue=6e-16,
Organism=Drosophila melanogaster, GI17137536, Length=229, Percent_Identity=29.2576419213974, Blast_Score=96, Evalue=2e-20,
Organism=Drosophila melanogaster, GI24648389, Length=196, Percent_Identity=28.0612244897959, Blast_Score=86, Evalue=2e-17,
Organism=Drosophila melanogaster, GI28571445, Length=193, Percent_Identity=30.5699481865285, Blast_Score=74, Evalue=9e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003000 [H]

Pfam domain/function: PF02146 SIR2 [H]

EC number: 3.5.1.- [C]

Molecular weight: Translated: 26273; Mature: 26142

Theoretical pI: Translated: 5.68; Mature: 5.68

Prosite motif: PS50305 SIRTUIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
0.4 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAEFAADLIQTLRQTQSLVVLTGSGISAPSGIPTYRSAAADARWTAYDPDKVATFAGFER
CHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCCHHHHHCCCCCCCCCCHHHHHHCCCCC
DPVGVFQVYQAMKRQCEAAQPNAGHYALAQLEQLGTQFKLFTQNIDSLHQRAGSSQVYEV
CCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHH
HGSLARTICSREGNLVETWNAEQPICPACGAPLRPDIVWFGELLDAGILQAAKAAFDSSD
HHHHHHHHHHCCCCEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCC
VALVIGTSAIVEPIASLPHRALRRKKTVIEINPDIPLRGIATFSLAGSADELLPQLIKEV
EEEEECCHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCEEEEEECCCHHHHHHHHHHHH
WKL
HCC
>Mature Secondary Structure 
AEFAADLIQTLRQTQSLVVLTGSGISAPSGIPTYRSAAADARWTAYDPDKVATFAGFER
HHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCCHHHHHCCCCCCCCCCHHHHHHCCCCC
DPVGVFQVYQAMKRQCEAAQPNAGHYALAQLEQLGTQFKLFTQNIDSLHQRAGSSQVYEV
CCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHH
HGSLARTICSREGNLVETWNAEQPICPACGAPLRPDIVWFGELLDAGILQAAKAAFDSSD
HHHHHHHHHHCCCCEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCC
VALVIGTSAIVEPIASLPHRALRRKKTVIEINPDIPLRGIATFSLAGSADELLPQLIKEV
EEEEECCHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCEEEEEECCCHHHHHHHHHHHH
WKL
HCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9389475; 10841563; 12408821; 11336664; 11336676; 12091395 [H]