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The map label for this gene is tgt

Identifier: 159897161

GI number: 159897161

Start: 729779

End: 730957

Strand: Direct

Name: tgt

Synonym: Haur_0632

Alternate gene names: 159897161

Gene position: 729779-730957 (Clockwise)

Preceding gene: 159897159

Following gene: 159897162

Centisome position: 11.5

GC content: 52.5

Gene sequence:

>1179_bases
ATGAACAACTTTACTTACACCCTTGAACATAGCGATGGTGAGGCGCGGGCAGGCCAATTCAGCACGCCGCATGGCACCAT
TCAAACGCCTGTCTTTATGCCGGTTGGCACCCAAGCCACGGTGAAAACGCTTGATCCCTTGGAAGTCGAGGCGATCGGCT
CACAAATTATCTTATCGAATACCTATCATTTATATTTGCGCCCAAGCGCCGATTTGGTGGCTGAAATGGGCGGCTTGCAT
CGTTTTATGCAATGGCCTAAGCCAATTCTGACCGATTCGGGAGGCTTTCAGGTGTTTAGCTTGGGGCCGCATAGCAAAAT
TGATGAAGATGGCGTAACCTTCAAATCGCATATCGATGGCTCGAAACATCGCTTTACCCCCGAAAGCGCGATTGGCATTC
AAGAAAAATTGGGCGCTGATATTATTATGGCCTTTGATGAATGTGCCCCCCAACCCACGACCCATGCCTATACCAAAGCT
GCCATGGAGCGCACTCATCGTTGGTTATTACGCTGTATTGCCGCCAAAACCCGTGCGGATCAAGCGCTATTTGGGATTGT
GCAGGGTGGGGTTGAGGCCGATTTGCGCCGCGAAAGCGCGAGCTTTATCGCCCAACAAGATGTGCCTGGCATCGGTATTG
GTGGCCTGAGCGTTGGCGAACCCAAAGAGCAAATGTATGGCATGCTCGAAGAAACTACGCCCTTGCTACCGCGCAACAAG
CCGCGCTACCTGATGGGTGTTGGTTCACCCGAAGATTTGCTCGAAGGCGTGGCGCGTGGGGTCGATATGTTTGATTGCGT
GTTGCCAACTCGTTTGGGGCGTAATGGAGCCTTATTCATTCCCGAAGGGCGCTTGAATATTGGCAACGCCAAATATGCCC
GCGAAGATGCGCCAATTGATGCAACGTGCGATTGCTCGACCTGCCAACGTTTTAGCCGCGCCTATCTGCGCCACTTGTTC
CGTACCGAGGAAGTGCTTGGGCTACGCTTGGCAACGCTGCATAATCTGCGTTTTTTAATTCGGCTGATGGAACAAGCACG
TGAGGCAATTCTGCAAGATCGCTATCAAAGCTTTATGGACGATTGGCTCAGCCGCTTTCAAACCATCCCGCATGCTGTGC
GCGAAGCCAGCCGTGCAGCCCGATTAAACTCATTACGCACCCAAGGGGACAAGGCATGA

Upstream 100 bases:

>100_bases
GCAGATCAAATCAGGCATTCAACCGATTGGCTTTTGTTACCAAATCCTTATACTCAGAGCTTTTTCAGCCAGCCAAAATA
GCGCTATAATTGTCCACATT

Downstream 100 bases:

>100_bases
GCGGCGTGACCGTTCGTTCAACCGAAGAATATACCCGCATCGCTTGGCGCTTGATCATTTTGAGCTTTCTGGCGTTTTGT
TTTGTGATCGCAAGTAGTTG

Product: queuine tRNA-ribosyltransferase

Products: NA

Alternate protein names: Guanine insertion enzyme; tRNA-guanine transglycosylase

Number of amino acids: Translated: 392; Mature: 392

Protein sequence:

>392_residues
MNNFTYTLEHSDGEARAGQFSTPHGTIQTPVFMPVGTQATVKTLDPLEVEAIGSQIILSNTYHLYLRPSADLVAEMGGLH
RFMQWPKPILTDSGGFQVFSLGPHSKIDEDGVTFKSHIDGSKHRFTPESAIGIQEKLGADIIMAFDECAPQPTTHAYTKA
AMERTHRWLLRCIAAKTRADQALFGIVQGGVEADLRRESASFIAQQDVPGIGIGGLSVGEPKEQMYGMLEETTPLLPRNK
PRYLMGVGSPEDLLEGVARGVDMFDCVLPTRLGRNGALFIPEGRLNIGNAKYAREDAPIDATCDCSTCQRFSRAYLRHLF
RTEEVLGLRLATLHNLRFLIRLMEQAREAILQDRYQSFMDDWLSRFQTIPHAVREASRAARLNSLRTQGDKA

Sequences:

>Translated_392_residues
MNNFTYTLEHSDGEARAGQFSTPHGTIQTPVFMPVGTQATVKTLDPLEVEAIGSQIILSNTYHLYLRPSADLVAEMGGLH
RFMQWPKPILTDSGGFQVFSLGPHSKIDEDGVTFKSHIDGSKHRFTPESAIGIQEKLGADIIMAFDECAPQPTTHAYTKA
AMERTHRWLLRCIAAKTRADQALFGIVQGGVEADLRRESASFIAQQDVPGIGIGGLSVGEPKEQMYGMLEETTPLLPRNK
PRYLMGVGSPEDLLEGVARGVDMFDCVLPTRLGRNGALFIPEGRLNIGNAKYAREDAPIDATCDCSTCQRFSRAYLRHLF
RTEEVLGLRLATLHNLRFLIRLMEQAREAILQDRYQSFMDDWLSRFQTIPHAVREASRAARLNSLRTQGDKA
>Mature_392_residues
MNNFTYTLEHSDGEARAGQFSTPHGTIQTPVFMPVGTQATVKTLDPLEVEAIGSQIILSNTYHLYLRPSADLVAEMGGLH
RFMQWPKPILTDSGGFQVFSLGPHSKIDEDGVTFKSHIDGSKHRFTPESAIGIQEKLGADIIMAFDECAPQPTTHAYTKA
AMERTHRWLLRCIAAKTRADQALFGIVQGGVEADLRRESASFIAQQDVPGIGIGGLSVGEPKEQMYGMLEETTPLLPRNK
PRYLMGVGSPEDLLEGVARGVDMFDCVLPTRLGRNGALFIPEGRLNIGNAKYAREDAPIDATCDCSTCQRFSRAYLRHLF
RTEEVLGLRLATLHNLRFLIRLMEQAREAILQDRYQSFMDDWLSRFQTIPHAVREASRAARLNSLRTQGDKA

Specific function: Exchanges the guanine residue with 7-aminomethyl-7- deazaguanine in tRNAs with GU(N) anticodons (tRNA-Asp, -Asn, -His and -Tyr). After this exchange, a cyclopentendiol moiety is attached to the 7-aminomethyl group of 7-deazaguanine, resulting in the hyper

COG id: COG0343

COG function: function code J; Queuine/archaeosine tRNA-ribosyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the queuine tRNA-ribosyltransferase family

Homologues:

Organism=Homo sapiens, GI13654276, Length=356, Percent_Identity=46.6292134831461, Blast_Score=338, Evalue=4e-93,
Organism=Escherichia coli, GI1786607, Length=365, Percent_Identity=51.5068493150685, Blast_Score=377, Evalue=1e-106,
Organism=Caenorhabditis elegans, GI17544680, Length=355, Percent_Identity=43.6619718309859, Blast_Score=297, Evalue=8e-81,
Organism=Caenorhabditis elegans, GI25146502, Length=249, Percent_Identity=23.6947791164659, Blast_Score=76, Evalue=3e-14,
Organism=Drosophila melanogaster, GI19920510, Length=385, Percent_Identity=45.1948051948052, Blast_Score=342, Evalue=2e-94,
Organism=Drosophila melanogaster, GI21355311, Length=215, Percent_Identity=24.6511627906977, Blast_Score=80, Evalue=3e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TGT_HERA2 (A9AW42)

Other databases:

- EMBL:   CP000875
- RefSeq:   YP_001543408.1
- ProteinModelPortal:   A9AW42
- SMR:   A9AW42
- GeneID:   5732530
- GenomeReviews:   CP000875_GR
- KEGG:   hau:Haur_0632
- HOGENOM:   HBG629929
- OMA:   FMPVGTV
- ProtClustDB:   CLSK2796133
- BioCyc:   HAUR316274:HAUR_0632-MONOMER
- HAMAP:   MF_00168
- InterPro:   IPR004803
- InterPro:   IPR002616
- Gene3D:   G3DSA:3.20.20.105
- PANTHER:   PTHR11962
- TIGRFAMs:   TIGR00430
- TIGRFAMs:   TIGR00449

Pfam domain/function: PF01702 TGT; SSF51713 tRNA_ribo_trans

EC number: =2.4.2.29

Molecular weight: Translated: 43590; Mature: 43590

Theoretical pI: Translated: 6.79; Mature: 6.79

Prosite motif: NA

Important sites: ACT_SITE 92-92 BINDING 93-93

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNNFTYTLEHSDGEARAGQFSTPHGTIQTPVFMPVGTQATVKTLDPLEVEAIGSQIILSN
CCCCEEEEECCCCCCCCCCCCCCCCCCCCCEEEECCCCHHHHCCCCCHHHHCCCEEEEEC
TYHLYLRPSADLVAEMGGLHRFMQWPKPILTDSGGFQVFSLGPHSKIDEDGVTFKSHIDG
CEEEEECCCHHHHHHHHHHHHHHHCCCCCEECCCCEEEEECCCCCCCCCCCCEEHHCCCC
SKHRFTPESAIGIQEKLGADIIMAFDECAPQPTTHAYTKAAMERTHRWLLRCIAAKTRAD
CCCCCCCHHHCCHHHHHCCHHEEEEHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
QALFGIVQGGVEADLRRESASFIAQQDVPGIGIGGLSVGEPKEQMYGMLEETTPLLPRNK
HHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCC
PRYLMGVGSPEDLLEGVARGVDMFDCVLPTRLGRNGALFIPEGRLNIGNAKYAREDAPID
CCEEEECCCHHHHHHHHHCCCHHHHHHHHHHCCCCCCEEEECCCCCCCCCHHCCCCCCCC
ATCDCSTCQRFSRAYLRHLFRTEEVLGLRLATLHNLRFLIRLMEQAREAILQDRYQSFMD
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DWLSRFQTIPHAVREASRAARLNSLRTQGDKA
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MNNFTYTLEHSDGEARAGQFSTPHGTIQTPVFMPVGTQATVKTLDPLEVEAIGSQIILSN
CCCCEEEEECCCCCCCCCCCCCCCCCCCCCEEEECCCCHHHHCCCCCHHHHCCCEEEEEC
TYHLYLRPSADLVAEMGGLHRFMQWPKPILTDSGGFQVFSLGPHSKIDEDGVTFKSHIDG
CEEEEECCCHHHHHHHHHHHHHHHCCCCCEECCCCEEEEECCCCCCCCCCCCEEHHCCCC
SKHRFTPESAIGIQEKLGADIIMAFDECAPQPTTHAYTKAAMERTHRWLLRCIAAKTRAD
CCCCCCCHHHCCHHHHHCCHHEEEEHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
QALFGIVQGGVEADLRRESASFIAQQDVPGIGIGGLSVGEPKEQMYGMLEETTPLLPRNK
HHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCC
PRYLMGVGSPEDLLEGVARGVDMFDCVLPTRLGRNGALFIPEGRLNIGNAKYAREDAPID
CCEEEECCCHHHHHHHHHCCCHHHHHHHHHHCCCCCCEEEECCCCCCCCCHHCCCCCCCC
ATCDCSTCQRFSRAYLRHLFRTEEVLGLRLATLHNLRFLIRLMEQAREAILQDRYQSFMD
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DWLSRFQTIPHAVREASRAARLNSLRTQGDKA
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA