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The map label for this gene is folC [H]

Identifier: 159897132

GI number: 159897132

Start: 693956

End: 695263

Strand: Direct

Name: folC [H]

Synonym: Haur_0603

Alternate gene names: 159897132

Gene position: 693956-695263 (Clockwise)

Preceding gene: 159897131

Following gene: 159897133

Centisome position: 10.93

GC content: 51.38

Gene sequence:

>1308_bases
ATGCTGCAAACATATCAAGATGCCATGGATTGGATCTACAGTTTCCTCGATAGCGAGAAGAAGTTACCCAAAAATCCGAC
CGAATTTAATTTGCCCCGGGTCAAGGCGATGCTGGAGTTGTTGGGTAATCCACAGTTGCGCTATCCGGCGGTGATTGTGG
CTGGCACGAAGGGCAAAGGTTCAACGTGTGCCTTTTTAGAGTCGATTATCCGCCAGAGTGGCCTAAAAGTTGGCTTTTTT
AGCTCGCCGCACCTACACTCCTACCGCGAACGCATGCAAATCAATCGTGAGCTAATTAGCCAAAGCCAGCTTGTCGATTT
GATTAATCAGGTGCGACCGCTACTGGAGCCACTTGACCCCGAAGTTGGTTCACCAACCACCTATGAAATTGGGGTGGTTT
TGGGTTTGTACTACTTTGCTACGCAGGCAATCGAGTTGGCAGTACTTGAAATTGGCTTGGGTGGGCGCTATGATGCGATT
AACACTGTTAACCCTGTGCTCAGCGTAATTGCTTCGATCAGCTATGATCACACGGCGATTTTAGGCGATACACTAGCCAA
AATTGCCTATGAAAAGGCTGGAATCATCAAGCAGCATGTGCCAGTAATTAGCACTGTGCAGCAAGCCGAGGCGGCTGAGG
TGATCGCTCAGGTTGCTGCTGAGCAAGCGGCTCCGTTATTTATTGCAGGGATGGCGGGATTGCAGGAGCAAGCCTCAGGG
GTGGTTGCTGAATATCCACTGGTGATTGTGCCTGAACAACTGGGCTTGAAGGGTGAGTTTCAGATGCAAAATGCCCAACT
TGCCACCAGCGCCGCATTGCTCTTGCGCGAACTCGGTTTCCCCATTAGCGATGATGCTATTCGCCAAGGTTTGGCAACAA
CCCAATGGCCTGCTCGGTTTGAGCAAATTGCCAACGAACCGTTGACGCTTGTCGATGGAGCGCATAACGGCGATTCGGCG
CGGGTGCTGCTGCAAGCGCTCAAACAGAATTATTCAAACCGACCGCTGACCTTAGTTTTAGGCACATCAAGCGACAAAGA
TATTCAAGCAATTATTCAGCAACTCAGCAATTCGGCAACCCACCTGATCACAACCTGTTCGCGGCATCCACGGGCTTTAG
CACCCGAAAAACTGGCTGAATTGATTCAGCAGCATGTTAATCGCCCGGTTCATCAGACCAACAGCGTGGCCGAGGCCTTA
ACGCTGGCCCAACAACTCACATCCGCCGCTGGCCTCATTTGCGTAACTGGATCGCTGTTTGTGGCTGCCGAAGCGCGTGA
AACCCATGGCTTAGCCCAGCCCGATTAG

Upstream 100 bases:

>100_bases
TAGGGAATGAGGGCATGCTGACGAACTATCGATAAAAATGCACATAAACCGCTATTGGTTGTACACTAAGCAGCAGATGA
TTTAGCTGGAGCAAGCGTCG

Downstream 100 bases:

>100_bases
CCGATCGTAAACTGACTCGTAATGCCAAATCGCGACACTAGCAACAGCAGAGCAGCCCTCGCGATGCGGCATGGAGCAAC
GAGCATTGGAGTTTGTATGC

Product: FolC bifunctional protein

Products: NA

Alternate protein names: Folylpoly-gamma-glutamate synthetase; FPGS; Tetrahydrofolate synthase; Tetrahydrofolylpolyglutamate synthase [H]

Number of amino acids: Translated: 435; Mature: 435

Protein sequence:

>435_residues
MLQTYQDAMDWIYSFLDSEKKLPKNPTEFNLPRVKAMLELLGNPQLRYPAVIVAGTKGKGSTCAFLESIIRQSGLKVGFF
SSPHLHSYRERMQINRELISQSQLVDLINQVRPLLEPLDPEVGSPTTYEIGVVLGLYYFATQAIELAVLEIGLGGRYDAI
NTVNPVLSVIASISYDHTAILGDTLAKIAYEKAGIIKQHVPVISTVQQAEAAEVIAQVAAEQAAPLFIAGMAGLQEQASG
VVAEYPLVIVPEQLGLKGEFQMQNAQLATSAALLLRELGFPISDDAIRQGLATTQWPARFEQIANEPLTLVDGAHNGDSA
RVLLQALKQNYSNRPLTLVLGTSSDKDIQAIIQQLSNSATHLITTCSRHPRALAPEKLAELIQQHVNRPVHQTNSVAEAL
TLAQQLTSAAGLICVTGSLFVAAEARETHGLAQPD

Sequences:

>Translated_435_residues
MLQTYQDAMDWIYSFLDSEKKLPKNPTEFNLPRVKAMLELLGNPQLRYPAVIVAGTKGKGSTCAFLESIIRQSGLKVGFF
SSPHLHSYRERMQINRELISQSQLVDLINQVRPLLEPLDPEVGSPTTYEIGVVLGLYYFATQAIELAVLEIGLGGRYDAI
NTVNPVLSVIASISYDHTAILGDTLAKIAYEKAGIIKQHVPVISTVQQAEAAEVIAQVAAEQAAPLFIAGMAGLQEQASG
VVAEYPLVIVPEQLGLKGEFQMQNAQLATSAALLLRELGFPISDDAIRQGLATTQWPARFEQIANEPLTLVDGAHNGDSA
RVLLQALKQNYSNRPLTLVLGTSSDKDIQAIIQQLSNSATHLITTCSRHPRALAPEKLAELIQQHVNRPVHQTNSVAEAL
TLAQQLTSAAGLICVTGSLFVAAEARETHGLAQPD
>Mature_435_residues
MLQTYQDAMDWIYSFLDSEKKLPKNPTEFNLPRVKAMLELLGNPQLRYPAVIVAGTKGKGSTCAFLESIIRQSGLKVGFF
SSPHLHSYRERMQINRELISQSQLVDLINQVRPLLEPLDPEVGSPTTYEIGVVLGLYYFATQAIELAVLEIGLGGRYDAI
NTVNPVLSVIASISYDHTAILGDTLAKIAYEKAGIIKQHVPVISTVQQAEAAEVIAQVAAEQAAPLFIAGMAGLQEQASG
VVAEYPLVIVPEQLGLKGEFQMQNAQLATSAALLLRELGFPISDDAIRQGLATTQWPARFEQIANEPLTLVDGAHNGDSA
RVLLQALKQNYSNRPLTLVLGTSSDKDIQAIIQQLSNSATHLITTCSRHPRALAPEKLAELIQQHVNRPVHQTNSVAEAL
TLAQQLTSAAGLICVTGSLFVAAEARETHGLAQPD

Specific function: Conversion of folates to polyglutamate derivatives. It preferes 5,10-methylenetetrahydrofolate, rather than 10- formyltetrahydrofolate as folate substrate [H]

COG id: COG0285

COG function: function code H; Folylpolyglutamate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the folylpolyglutamate synthase family [H]

Homologues:

Organism=Homo sapiens, GI66932990, Length=344, Percent_Identity=33.4302325581395, Blast_Score=172, Evalue=5e-43,
Organism=Homo sapiens, GI66932984, Length=340, Percent_Identity=33.5294117647059, Blast_Score=172, Evalue=5e-43,
Organism=Escherichia coli, GI1788654, Length=423, Percent_Identity=29.5508274231678, Blast_Score=156, Evalue=2e-39,
Organism=Caenorhabditis elegans, GI17553150, Length=317, Percent_Identity=31.2302839116719, Blast_Score=149, Evalue=4e-36,
Organism=Caenorhabditis elegans, GI17553148, Length=317, Percent_Identity=31.2302839116719, Blast_Score=149, Evalue=4e-36,
Organism=Caenorhabditis elegans, GI71984923, Length=317, Percent_Identity=31.2302839116719, Blast_Score=148, Evalue=5e-36,
Organism=Saccharomyces cerevisiae, GI6323760, Length=432, Percent_Identity=29.8611111111111, Blast_Score=171, Evalue=2e-43,
Organism=Saccharomyces cerevisiae, GI6324815, Length=427, Percent_Identity=27.6346604215457, Blast_Score=118, Evalue=2e-27,
Organism=Drosophila melanogaster, GI24641571, Length=329, Percent_Identity=32.8267477203647, Blast_Score=150, Evalue=2e-36,
Organism=Drosophila melanogaster, GI24581568, Length=179, Percent_Identity=36.3128491620112, Blast_Score=121, Evalue=1e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018109
- InterPro:   IPR001645
- InterPro:   IPR004101
- InterPro:   IPR013221 [H]

Pfam domain/function: PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: =6.3.2.17 [H]

Molecular weight: Translated: 47124; Mature: 47124

Theoretical pI: Translated: 5.40; Mature: 5.40

Prosite motif: PS01011 FOLYLPOLYGLU_SYNT_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLQTYQDAMDWIYSFLDSEKKLPKNPTEFNLPRVKAMLELLGNPQLRYPAVIVAGTKGKG
CCCHHHHHHHHHHHHHCCHHCCCCCCCCCCCHHHHHHHHHHCCCCCCCCEEEEECCCCCC
STCAFLESIIRQSGLKVGFFSSPHLHSYRERMQINRELISQSQLVDLINQVRPLLEPLDP
CHHHHHHHHHHHCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
EVGSPTTYEIGVVLGLYYFATQAIELAVLEIGLGGRYDAINTVNPVLSVIASISYDHTAI
CCCCCCCCHHHHHHHHHHHHHHHHHHHHEEECCCCCCCCHHHHHHHHHHHHHHCCCCHHH
LGDTLAKIAYEKAGIIKQHVPVISTVQQAEAAEVIAQVAAEQAAPLFIAGMAGLQEQASG
HHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHCCCEEEHHHHHHHHHHCC
VVAEYPLVIVPEQLGLKGEFQMQNAQLATSAALLLRELGFPISDDAIRQGLATTQWPARF
CEEECCEEEECHHHCCCCCEEECCHHHHHHHHHHHHHHCCCCCHHHHHCCCCCCCCHHHH
EQIANEPLTLVDGAHNGDSARVLLQALKQNYSNRPLTLVLGTSSDKDIQAIIQQLSNSAT
HHHCCCCEEEECCCCCCHHHHHHHHHHHHCCCCCCEEEEEECCCCHHHHHHHHHHHCCHH
HLITTCSRHPRALAPEKLAELIQQHVNRPVHQTNSVAEALTLAQQLTSAAGLICVTGSLF
HHHHHHCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCEEEEECCEE
VAAEARETHGLAQPD
EEECCHHHCCCCCCC
>Mature Secondary Structure
MLQTYQDAMDWIYSFLDSEKKLPKNPTEFNLPRVKAMLELLGNPQLRYPAVIVAGTKGKG
CCCHHHHHHHHHHHHHCCHHCCCCCCCCCCCHHHHHHHHHHCCCCCCCCEEEEECCCCCC
STCAFLESIIRQSGLKVGFFSSPHLHSYRERMQINRELISQSQLVDLINQVRPLLEPLDP
CHHHHHHHHHHHCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
EVGSPTTYEIGVVLGLYYFATQAIELAVLEIGLGGRYDAINTVNPVLSVIASISYDHTAI
CCCCCCCCHHHHHHHHHHHHHHHHHHHHEEECCCCCCCCHHHHHHHHHHHHHHCCCCHHH
LGDTLAKIAYEKAGIIKQHVPVISTVQQAEAAEVIAQVAAEQAAPLFIAGMAGLQEQASG
HHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHCCCEEEHHHHHHHHHHCC
VVAEYPLVIVPEQLGLKGEFQMQNAQLATSAALLLRELGFPISDDAIRQGLATTQWPARF
CEEECCEEEECHHHCCCCCEEECCHHHHHHHHHHHHHHCCCCCHHHHHCCCCCCCCHHHH
EQIANEPLTLVDGAHNGDSARVLLQALKQNYSNRPLTLVLGTSSDKDIQAIIQQLSNSAT
HHHCCCCEEEECCCCCCHHHHHHHHHHHHCCCCCCEEEEEECCCCHHHHHHHHHHHCCHH
HLITTCSRHPRALAPEKLAELIQQHVNRPVHQTNSVAEALTLAQQLTSAAGLICVTGSLF
HHHHHHCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCEEEEECCEE
VAAEARETHGLAQPD
EEECCHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8419299; 9384377; 2553669 [H]