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The map label for this gene is infB [H]

Identifier: 159897008

GI number: 159897008

Start: 554627

End: 556435

Strand: Direct

Name: infB [H]

Synonym: Haur_0476

Alternate gene names: 159897008

Gene position: 554627-556435 (Clockwise)

Preceding gene: 159897006

Following gene: 159897009

Centisome position: 8.74

GC content: 52.74

Gene sequence:

>1809_bases
ATGGCTCGCCCAGTTGCTCCCGCCCGCCCACGTGGCCCAGTTGAGCTTGGGCCGGTCATGACGGTGCGTGAGTTATCCGA
AGCACTCAGTGTTGGCGCAGCCGATATTATCAAAGAAATGCTCAAGCAAGGCATTTTGGCGAATATTAATCAGCAGCTTG
ATTATGAAACTGCCGCCGTGATTGCCGCCGAGTTTGATATCGAAACCTCTGAGCATGTGCCAGAACGGATGGCTGGCTTG
GTTGATGATATTGGCGATGCGTTGCGGGCTGAAACAGCTGAAAATCTCAAAACCCGCCCACCAGTCGTCACGATCATGGG
TCACGTTGACCACGGTAAGACCAAATTGCTCGATGCTGTGCGCTCAACCCGCGTGGCGGAAGGCGAAGCTGGCGGGATTA
CCCAGCACATTGGCGCGTATCAGGTTGAAATCCATGGCCGCAAAATTTCGTTCTTGGATACGCCTGGTCACGAAGCGTTT
ACCGCAATGCGGGCACGGGGAGCCACGGTTACCGATATTGTTATTTTGGTGGTAGCTGCCGACGACGGTGTGATGCCGCA
AACGGCTGAGGCAATTGCCCACGTCAAAGCGGCTGGTGTGCCAATGATCGTGGCAATCAACAAAATCGATGTGCCTGGAG
CCAATCCCGACCGCGTGCGCCAACAACTTGCTGGCGAAGGTGTAACTACCGAATCGTGGGGCGGCGATGTGCCCGATGTC
GAAATTTCGGCCAAGCACAAGAAAAATATCGATGGCCTGTTGGATATGGTCTTGTTGGTCGCCGATTTGCAAGAATTGAA
GGCCAACCCAGACAAACCAGCAATCGGTACAATCGTTGAAGCTGAGCTTGATAAAGGCCGTGGCCCAGTCGCAACCGTGC
TGATTCAAAACGGCACGCTGCGCCAAGATGATATTGTGGTGGTCGGGGCAACCCACGGCAAGATTCGCGCAATGTTTACC
GACATTGGCCGCCGGATTCGCATCGCTGAGCCAGCCACACCAGTCTCGATCATTGGGCTTTCAGAAGTGCCACAGGCTGG
CGATATTTTGCAAGTGCTCGATGATCCACGGATTGCCCGCGAGGTCGCAACGGTACGCCAACGCCAACGCCAAATGGAGC
ATATGGCCAATCAGCTTGGCAAAGCTACCACGCTTGATGAAGTGTACAAACAAATTCAAGCAGGCAAAATCAAAGATCTC
AATATTATTCTCAAGGCTGACGTGCAAGGCTCAATTGGGGCAATCGAACACTCATTGGCTCAGTTGAACGAGAAACAAAG
CGAGATCCAAATCAAGATTTTGCACCGTGGTACTGGCACAATCAGCGAATCGGACGTGAGCTTGGCCGTGGCTTCGCACG
CGATTATCATTGGCTTTAATGCTCGGCCCGATGCTGCTGCACGCCGCAGCGCTGAAGCCAACGGGATTGATATTCGCTTC
TACAACATCATTTACCAATTGATCGAAGACCTCAACAAGGCCATGATCGGGATGCTCGATCCTGAGATCAAAGAAGTAAC
CGATGGCTTTGCTGAAGTGCGCAATACCTTCCGCTTGCCATCACGCGAAGTTGTGGCTGGCTTGATGATGATCGACGGTA
AAGTTACCCGTAACTCATTGGTACGGGTCTTGCGCTCAGGGGTCGTGCTGCACGATGGCCGAATTGGCTCGCTGCGCCGT
TTCAAAGACGATGTGCGCGAAGTGCTTTCAGGCTATGAATGTGGGATTCAGATCGATGGCTTCAACGACATCGAAACTGG
CGATACCATGGAATTCTATCGCAAGGAAAAGATTATTCGTACCTCGTAA

Upstream 100 bases:

>100_bases
CGTTCCACCTCGTGGTAGCGGTGGCGTTCCACCCCGGGGCAGCGGTGGCCGTGGCCAAAACAACAATCAGCAAGGCGGTC
GTGGCCGTGGTACGCCAATT

Downstream 100 bases:

>100_bases
GCTGAATTAAATTCTAGCGTGGAGACAAATAGATCTGTTGGCGACAGCAGATATATTTGTCTCCATTCTGCATTATCACT
ACCGTGGGAGTGACTGTTAT

Product: translation initiation factor IF-2

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 602; Mature: 601

Protein sequence:

>602_residues
MARPVAPARPRGPVELGPVMTVRELSEALSVGAADIIKEMLKQGILANINQQLDYETAAVIAAEFDIETSEHVPERMAGL
VDDIGDALRAETAENLKTRPPVVTIMGHVDHGKTKLLDAVRSTRVAEGEAGGITQHIGAYQVEIHGRKISFLDTPGHEAF
TAMRARGATVTDIVILVVAADDGVMPQTAEAIAHVKAAGVPMIVAINKIDVPGANPDRVRQQLAGEGVTTESWGGDVPDV
EISAKHKKNIDGLLDMVLLVADLQELKANPDKPAIGTIVEAELDKGRGPVATVLIQNGTLRQDDIVVVGATHGKIRAMFT
DIGRRIRIAEPATPVSIIGLSEVPQAGDILQVLDDPRIAREVATVRQRQRQMEHMANQLGKATTLDEVYKQIQAGKIKDL
NIILKADVQGSIGAIEHSLAQLNEKQSEIQIKILHRGTGTISESDVSLAVASHAIIIGFNARPDAAARRSAEANGIDIRF
YNIIYQLIEDLNKAMIGMLDPEIKEVTDGFAEVRNTFRLPSREVVAGLMMIDGKVTRNSLVRVLRSGVVLHDGRIGSLRR
FKDDVREVLSGYECGIQIDGFNDIETGDTMEFYRKEKIIRTS

Sequences:

>Translated_602_residues
MARPVAPARPRGPVELGPVMTVRELSEALSVGAADIIKEMLKQGILANINQQLDYETAAVIAAEFDIETSEHVPERMAGL
VDDIGDALRAETAENLKTRPPVVTIMGHVDHGKTKLLDAVRSTRVAEGEAGGITQHIGAYQVEIHGRKISFLDTPGHEAF
TAMRARGATVTDIVILVVAADDGVMPQTAEAIAHVKAAGVPMIVAINKIDVPGANPDRVRQQLAGEGVTTESWGGDVPDV
EISAKHKKNIDGLLDMVLLVADLQELKANPDKPAIGTIVEAELDKGRGPVATVLIQNGTLRQDDIVVVGATHGKIRAMFT
DIGRRIRIAEPATPVSIIGLSEVPQAGDILQVLDDPRIAREVATVRQRQRQMEHMANQLGKATTLDEVYKQIQAGKIKDL
NIILKADVQGSIGAIEHSLAQLNEKQSEIQIKILHRGTGTISESDVSLAVASHAIIIGFNARPDAAARRSAEANGIDIRF
YNIIYQLIEDLNKAMIGMLDPEIKEVTDGFAEVRNTFRLPSREVVAGLMMIDGKVTRNSLVRVLRSGVVLHDGRIGSLRR
FKDDVREVLSGYECGIQIDGFNDIETGDTMEFYRKEKIIRTS
>Mature_601_residues
ARPVAPARPRGPVELGPVMTVRELSEALSVGAADIIKEMLKQGILANINQQLDYETAAVIAAEFDIETSEHVPERMAGLV
DDIGDALRAETAENLKTRPPVVTIMGHVDHGKTKLLDAVRSTRVAEGEAGGITQHIGAYQVEIHGRKISFLDTPGHEAFT
AMRARGATVTDIVILVVAADDGVMPQTAEAIAHVKAAGVPMIVAINKIDVPGANPDRVRQQLAGEGVTTESWGGDVPDVE
ISAKHKKNIDGLLDMVLLVADLQELKANPDKPAIGTIVEAELDKGRGPVATVLIQNGTLRQDDIVVVGATHGKIRAMFTD
IGRRIRIAEPATPVSIIGLSEVPQAGDILQVLDDPRIAREVATVRQRQRQMEHMANQLGKATTLDEVYKQIQAGKIKDLN
IILKADVQGSIGAIEHSLAQLNEKQSEIQIKILHRGTGTISESDVSLAVASHAIIIGFNARPDAAARRSAEANGIDIRFY
NIIYQLIEDLNKAMIGMLDPEIKEVTDGFAEVRNTFRLPSREVVAGLMMIDGKVTRNSLVRVLRSGVVLHDGRIGSLRRF
KDDVREVLSGYECGIQIDGFNDIETGDTMEFYRKEKIIRTS

Specific function: One of the essential components for the initiation of protein synthesis. Protects formylmethionyl-tRNA from spontaneous hydrolysis and promotes its binding to the 30S ribosomal subunits. Also involved in the hydrolysis of GTP during the formation of the 7

COG id: COG0532

COG function: function code J; Translation initiation factor 2 (IF-2; GTPase)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the IF-2 family [H]

Homologues:

Organism=Homo sapiens, GI53729339, Length=556, Percent_Identity=39.2086330935252, Blast_Score=361, Evalue=1e-99,
Organism=Homo sapiens, GI53729337, Length=556, Percent_Identity=39.2086330935252, Blast_Score=361, Evalue=1e-99,
Organism=Homo sapiens, GI84043963, Length=143, Percent_Identity=43.3566433566434, Blast_Score=117, Evalue=3e-26,
Organism=Homo sapiens, GI157426893, Length=251, Percent_Identity=27.4900398406374, Blast_Score=75, Evalue=2e-13,
Organism=Escherichia coli, GI1789559, Length=589, Percent_Identity=49.9151103565365, Blast_Score=537, Evalue=1e-154,
Organism=Escherichia coli, GI2367247, Length=128, Percent_Identity=42.96875, Blast_Score=82, Evalue=9e-17,
Organism=Escherichia coli, GI48994988, Length=137, Percent_Identity=37.2262773722628, Blast_Score=74, Evalue=2e-14,
Organism=Escherichia coli, GI1788922, Length=232, Percent_Identity=30.1724137931034, Blast_Score=71, Evalue=2e-13,
Organism=Escherichia coli, GI1790412, Length=133, Percent_Identity=36.8421052631579, Blast_Score=67, Evalue=4e-12,
Organism=Escherichia coli, GI1789737, Length=133, Percent_Identity=36.8421052631579, Blast_Score=67, Evalue=4e-12,
Organism=Caenorhabditis elegans, GI71994658, Length=554, Percent_Identity=34.8375451263538, Blast_Score=295, Evalue=3e-80,
Organism=Caenorhabditis elegans, GI212656558, Length=599, Percent_Identity=24.0400667779633, Blast_Score=117, Evalue=1e-26,
Organism=Caenorhabditis elegans, GI17557151, Length=217, Percent_Identity=29.9539170506912, Blast_Score=81, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI25141371, Length=241, Percent_Identity=27.3858921161826, Blast_Score=72, Evalue=8e-13,
Organism=Caenorhabditis elegans, GI17556456, Length=244, Percent_Identity=29.9180327868852, Blast_Score=67, Evalue=3e-11,
Organism=Saccharomyces cerevisiae, GI6324550, Length=622, Percent_Identity=35.048231511254, Blast_Score=323, Evalue=6e-89,
Organism=Saccharomyces cerevisiae, GI6319282, Length=480, Percent_Identity=25.2083333333333, Blast_Score=116, Evalue=9e-27,
Organism=Saccharomyces cerevisiae, GI6324761, Length=132, Percent_Identity=36.3636363636364, Blast_Score=67, Evalue=6e-12,
Organism=Drosophila melanogaster, GI28572034, Length=536, Percent_Identity=40.1119402985075, Blast_Score=342, Evalue=4e-94,
Organism=Drosophila melanogaster, GI24656849, Length=467, Percent_Identity=26.7665952890792, Blast_Score=113, Evalue=3e-25,
Organism=Drosophila melanogaster, GI160714833, Length=75, Percent_Identity=69.3333333333333, Blast_Score=110, Evalue=2e-24,
Organism=Drosophila melanogaster, GI78706572, Length=221, Percent_Identity=29.4117647058824, Blast_Score=77, Evalue=5e-14,
Organism=Drosophila melanogaster, GI19922690, Length=225, Percent_Identity=28.8888888888889, Blast_Score=67, Evalue=5e-11,

Paralogues:

None

Copy number: 1150 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006847
- InterPro:   IPR000795
- InterPro:   IPR005225
- InterPro:   IPR000178
- InterPro:   IPR015760
- InterPro:   IPR023115
- InterPro:   IPR004161
- InterPro:   IPR009000 [H]

Pfam domain/function: PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2; PF11987 IF-2; PF04760 IF2_N [H]

EC number: NA

Molecular weight: Translated: 65234; Mature: 65103

Theoretical pI: Translated: 5.55; Mature: 5.55

Prosite motif: PS01176 IF2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MARPVAPARPRGPVELGPVMTVRELSEALSVGAADIIKEMLKQGILANINQQLDYETAAV
CCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHCCHHHHCHHHCCCHHHEE
IAAEFDIETSEHVPERMAGLVDDIGDALRAETAENLKTRPPVVTIMGHVDHGKTKLLDAV
EEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCHHHHHHHH
RSTRVAEGEAGGITQHIGAYQVEIHGRKISFLDTPGHEAFTAMRARGATVTDIVILVVAA
HHHHCCCCCCCCCHHHCCEEEEEEECCEEEEEECCCHHHHHHHHHCCCCCEEEEEEEEEC
DDGVMPQTAEAIAHVKAAGVPMIVAINKIDVPGANPDRVRQQLAGEGVTTESWGGDVPDV
CCCCCCHHHHHHHHHHHCCCEEEEEEEEECCCCCCHHHHHHHHHCCCCCCCCCCCCCCCC
EISAKHKKNIDGLLDMVLLVADLQELKANPDKPAIGTIVEAELDKGRGPVATVLIQNGTL
EECCHHHCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEHHHCCCCCCEEEEEEECCCC
RQDDIVVVGATHGKIRAMFTDIGRRIRIAEPATPVSIIGLSEVPQAGDILQVLDDPRIAR
CCCCEEEEECCCCHHHHHHHHHCCEEEEECCCCCEEEEECCCCCCCCCHHHHHCCHHHHH
EVATVRQRQRQMEHMANQLGKATTLDEVYKQIQAGKIKDLNIILKADVQGSIGAIEHSLA
HHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCEEEEEEECCCCCHHHHHHHHH
QLNEKQSEIQIKILHRGTGTISESDVSLAVASHAIIIGFNARPDAAARRSAEANGIDIRF
HHCCCCCCEEEEEEECCCCCCCCCCCEEEEECEEEEEEECCCCCHHHHCCCCCCCEEEHH
YNIIYQLIEDLNKAMIGMLDPEIKEVTDGFAEVRNTFRLPSREVVAGLMMIDGKVTRNSL
HHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCHHHH
VRVLRSGVVLHDGRIGSLRRFKDDVREVLSGYECGIQIDGFNDIETGDTMEFYRKEKIIR
HHHHHCCEEEECCCCCHHHHHHHHHHHHHCCCCCCEEECCCCCCCCCCHHHHHHHHHHCC
TS
CC
>Mature Secondary Structure 
ARPVAPARPRGPVELGPVMTVRELSEALSVGAADIIKEMLKQGILANINQQLDYETAAV
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHCCHHHHCHHHCCCHHHEE
IAAEFDIETSEHVPERMAGLVDDIGDALRAETAENLKTRPPVVTIMGHVDHGKTKLLDAV
EEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCHHHHHHHH
RSTRVAEGEAGGITQHIGAYQVEIHGRKISFLDTPGHEAFTAMRARGATVTDIVILVVAA
HHHHCCCCCCCCCHHHCCEEEEEEECCEEEEEECCCHHHHHHHHHCCCCCEEEEEEEEEC
DDGVMPQTAEAIAHVKAAGVPMIVAINKIDVPGANPDRVRQQLAGEGVTTESWGGDVPDV
CCCCCCHHHHHHHHHHHCCCEEEEEEEEECCCCCCHHHHHHHHHCCCCCCCCCCCCCCCC
EISAKHKKNIDGLLDMVLLVADLQELKANPDKPAIGTIVEAELDKGRGPVATVLIQNGTL
EECCHHHCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEHHHCCCCCCEEEEEEECCCC
RQDDIVVVGATHGKIRAMFTDIGRRIRIAEPATPVSIIGLSEVPQAGDILQVLDDPRIAR
CCCCEEEEECCCCHHHHHHHHHCCEEEEECCCCCEEEEECCCCCCCCCHHHHHCCHHHHH
EVATVRQRQRQMEHMANQLGKATTLDEVYKQIQAGKIKDLNIILKADVQGSIGAIEHSLA
HHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCEEEEEEECCCCCHHHHHHHHH
QLNEKQSEIQIKILHRGTGTISESDVSLAVASHAIIIGFNARPDAAARRSAEANGIDIRF
HHCCCCCCEEEEEEECCCCCCCCCCCEEEEECEEEEEEECCCCCHHHHCCCCCCCEEEHH
YNIIYQLIEDLNKAMIGMLDPEIKEVTDGFAEVRNTFRLPSREVVAGLMMIDGKVTRNSL
HHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCHHHH
VRVLRSGVVLHDGRIGSLRRFKDDVREVLSGYECGIQIDGFNDIETGDTMEFYRKEKIIR
HHHHHCCEEEECCCCCHHHHHHHHHHHHHCCCCCCEEECCCCCCCCCCHHHHHHHHHHCC
TS
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; In phosphorus-containing anhydrides [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA