Click here to switch to the map view.

The map label for this gene is mscL

Identifier: 15926928

GI number: 15926928

Start: 1346130

End: 1346492

Strand: Reverse

Name: mscL

Synonym: SA1182

Alternate gene names: 15926928

Gene position: 1346492-1346130 (Counterclockwise)

Preceding gene: 15926932

Following gene: 15926920

Centisome position: 47.84

GC content: 31.4

Gene sequence:

>363_bases
ATGTTAAAAGAATTCAAAGAGTTCGCCTTAAAAGGTAACGTCTTAGATTTAGCAATTGCTGTTGTGATGGGTGCAGCTTT
CAACAAGATTATATCTTCATTAGTAGAAAATATCATCATGCCATTAATTGGTAAAATTTTCGGATCAGTTGATTTTGCTA
AAGAATGGTCATTCTGGGGTATTAAATACGGTTTATTTATCCAATCTGTTATCGACTTTATTATCATCGCGTTTGCTTTA
TTCATCTTTGTTAAGATTGCAAATACATTAATGAAGAAAGAAGAAGCCGAAGAAGAAGCAGTTGTGGAAGAAAATGTTGT
GTTATTAACTGAAATCAGAGATTTATTGCGTGAGAAAAAATAA

Upstream 100 bases:

>100_bases
AGGTCTTTGCGATAATCAAATTTTCTTGAATAATCTATTTTCTAATTTTTTTATTTGTTGTATAATTGAGGTTACATTTA
AAAAAGAGAGGTTGAGTGTA

Downstream 100 bases:

>100_bases
TTTTTAAATAAAAAGAGATGGGCTAAGGTTGTTCTATACATTCCAAAGTACCATCTCTATTTTTTATGTTTTAATTTCTT
TTGAATCTTGTTGAACTCTG

Product: large-conductance mechanosensitive channel

Products: non-specific ion/solute [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 120; Mature: 120

Protein sequence:

>120_residues
MLKEFKEFALKGNVLDLAIAVVMGAAFNKIISSLVENIIMPLIGKIFGSVDFAKEWSFWGIKYGLFIQSVIDFIIIAFAL
FIFVKIANTLMKKEEAEEEAVVEENVVLLTEIRDLLREKK

Sequences:

>Translated_120_residues
MLKEFKEFALKGNVLDLAIAVVMGAAFNKIISSLVENIIMPLIGKIFGSVDFAKEWSFWGIKYGLFIQSVIDFIIIAFAL
FIFVKIANTLMKKEEAEEEAVVEENVVLLTEIRDLLREKK
>Mature_120_residues
MLKEFKEFALKGNVLDLAIAVVMGAAFNKIISSLVENIIMPLIGKIFGSVDFAKEWSFWGIKYGLFIQSVIDFIIIAFAL
FIFVKIANTLMKKEEAEEEAVVEENVVLLTEIRDLLREKK

Specific function: Channel that opens in response to stretch forces in the membrane lipid bilayer. May participate in the regulation of osmotic pressure changes within the cell

COG id: COG1970

COG function: function code M; Large-conductance mechanosensitive channel

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mscL family

Homologues:

Organism=Escherichia coli, GI1789686, Length=131, Percent_Identity=47.3282442748092, Blast_Score=117, Evalue=3e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MSCL_STAA1 (A7X204)

Other databases:

- EMBL:   AP009324
- RefSeq:   YP_001441926.1
- ProteinModelPortal:   A7X204
- SMR:   A7X204
- STRING:   A7X204
- EnsemblBacteria:   EBSTAT00000005254
- GeneID:   5559868
- GenomeReviews:   AP009324_GR
- KEGG:   saw:SAHV_1336
- eggNOG:   COG1970
- GeneTree:   EBGT00050000025511
- HOGENOM:   HBG727604
- OMA:   GANVLAW
- ProtClustDB:   PRK13954
- BioCyc:   SAUR418127:SAHV_1336-MONOMER
- HAMAP:   MF_00115
- InterPro:   IPR019823
- InterPro:   IPR001185
- InterPro:   IPR015089
- Gene3D:   G3DSA:1.20.5.220
- PRINTS:   PR01264
- TIGRFAMs:   TIGR00220

Pfam domain/function: PF01741 MscL; SSF81330 MS_channel

EC number: NA

Molecular weight: Translated: 13616; Mature: 13616

Theoretical pI: Translated: 4.74; Mature: 4.74

Prosite motif: PS01327 MSCL

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0xb681fb0)-; HASH(0xdd4d244)-;

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLKEFKEFALKGNVLDLAIAVVMGAAFNKIISSLVENIIMPLIGKIFGSVDFAKEWSFWG
CCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHH
IKYGLFIQSVIDFIIIAFALFIFVKIANTLMKKEEAEEEAVVEENVVLLTEIRDLLREKK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHCCH
>Mature Secondary Structure
MLKEFKEFALKGNVLDLAIAVVMGAAFNKIISSLVENIIMPLIGKIFGSVDFAKEWSFWG
CCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHH
IKYGLFIQSVIDFIIIAFALFIFVKIANTLMKKEEAEEEAVVEENVVLLTEIRDLLREKK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHCCH

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: non-specific ion/solute [Cytoplasm] [C]

Specific reaction: non-specific ion/solute [Cytoplasm] = non-specific ion/solute [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA