Click here to switch to the map view.

The map label for this gene is truB

Identifier: 15926854

GI number: 15926854

Start: 1263787

End: 1264704

Strand: Direct

Name: truB

Synonym: SA1114

Alternate gene names: 15926854

Gene position: 1263787-1264704 (Clockwise)

Preceding gene: 15926853

Following gene: 15926855

Centisome position: 44.9

GC content: 32.24

Gene sequence:

>918_bases
ATGTATAATGGGATATTACCAGTATATAAAGAGCGCGGTTTAACAAGTCATGACGTTGTATTCAAATTGCGTAAAATATT
AAAAACTAAAAAAATAGGTCACACGGGTACGCTTGATCCCGAAGTTGCAGGCGTGTTACCGGTATGTATAGGTAATGCAA
CGAGAGTTAGTGATTATGTTATGGATATGGGCAAAGCTTATGAAGCAACTGTATCGATAGGAAGAAGTACAACGACTGAA
GATCAAACGGGTGATACATTGGAAACAAAAGGTGTACACTCAGCAGATTTTAATAAGGACGATATTGACCGATTGTTAGA
AAATTTTAAAGGTGTTATTGAACAAATTCCGCCAATGTATTCATCAGTCAAGGTGAATGGCAAAAAATTATATGAATATG
CGCGTAATAATGAAACAGTTGAAAGACCAAAGCGTAAAGTTAATATTAAAGACATTGGGCGTATATCTGAATTAGATTTT
AAAGAAAATGAGTGTCATTTTAAAATACGCGTCATCTGTGGTAAAGGTACATATATTAGAACGCTAGCAACTGATATTGG
TGTGAAATTAGGCTTTCCGGCACATATGTCGAAATTAACACGAATCGAGTCTGGTGGATTTGTGTTGAAAGATAGCCTTA
CATTAGAACAAATAAAAGAACTTCATGAGCAGGATTCATTGCAAAATAAATTGTTTCCTTTAGAATATGGATTAAAGGGT
TTGCCAAGCATTAAAATTAAAGATTCGCACATAAAAAAACGTATTTTAAATGGGCAGAAATTTAATAAAAATGAATTTGA
TAACAAAATTAAAGACCAAATTGTATTTATTGATGATGATTCAGAAAAAGTATTAGCAATTTATATGGTACACCCTACAA
AAGAATCAGAAATTAAACCTAAAAAAGTCTTTAATTAA

Upstream 100 bases:

>100_bases
ATACATAGATGTGTAGAAATAGTTAACATTTTCCAGTTTTTTTATGAATAAATTCAGTTGATACGCTATTAAAATATATT
TTAAAAAAGAAGGTGACTAT

Downstream 100 bases:

>100_bases
AGGAGATAGAATTTATGAAAGTCATAGAAGTGACACATCCTATACAATCTAAACAGTATATTACAGAGGATGTTGCAATG
GCATTCGGATTTTTCGATGG

Product: tRNA pseudouridine synthase B

Products: pseudouridine 5'-phosphate; H2O

Alternate protein names: tRNA pseudouridine 55 synthase; Psi55 synthase; tRNA pseudouridylate synthase; tRNA-uridine isomerase

Number of amino acids: Translated: 305; Mature: 305

Protein sequence:

>305_residues
MYNGILPVYKERGLTSHDVVFKLRKILKTKKIGHTGTLDPEVAGVLPVCIGNATRVSDYVMDMGKAYEATVSIGRSTTTE
DQTGDTLETKGVHSADFNKDDIDRLLENFKGVIEQIPPMYSSVKVNGKKLYEYARNNETVERPKRKVNIKDIGRISELDF
KENECHFKIRVICGKGTYIRTLATDIGVKLGFPAHMSKLTRIESGGFVLKDSLTLEQIKELHEQDSLQNKLFPLEYGLKG
LPSIKIKDSHIKKRILNGQKFNKNEFDNKIKDQIVFIDDDSEKVLAIYMVHPTKESEIKPKKVFN

Sequences:

>Translated_305_residues
MYNGILPVYKERGLTSHDVVFKLRKILKTKKIGHTGTLDPEVAGVLPVCIGNATRVSDYVMDMGKAYEATVSIGRSTTTE
DQTGDTLETKGVHSADFNKDDIDRLLENFKGVIEQIPPMYSSVKVNGKKLYEYARNNETVERPKRKVNIKDIGRISELDF
KENECHFKIRVICGKGTYIRTLATDIGVKLGFPAHMSKLTRIESGGFVLKDSLTLEQIKELHEQDSLQNKLFPLEYGLKG
LPSIKIKDSHIKKRILNGQKFNKNEFDNKIKDQIVFIDDDSEKVLAIYMVHPTKESEIKPKKVFN
>Mature_305_residues
MYNGILPVYKERGLTSHDVVFKLRKILKTKKIGHTGTLDPEVAGVLPVCIGNATRVSDYVMDMGKAYEATVSIGRSTTTE
DQTGDTLETKGVHSADFNKDDIDRLLENFKGVIEQIPPMYSSVKVNGKKLYEYARNNETVERPKRKVNIKDIGRISELDF
KENECHFKIRVICGKGTYIRTLATDIGVKLGFPAHMSKLTRIESGGFVLKDSLTLEQIKELHEQDSLQNKLFPLEYGLKG
LPSIKIKDSHIKKRILNGQKFNKNEFDNKIKDQIVFIDDDSEKVLAIYMVHPTKESEIKPKKVFN

Specific function: Responsible for synthesis of pseudouridine from uracil- 55 in the psi GC loop of transfer RNAs

COG id: COG0130

COG function: function code J; Pseudouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pseudouridine synthase truB family. Type 1 subfamily

Homologues:

Organism=Homo sapiens, GI21040257, Length=260, Percent_Identity=34.2307692307692, Blast_Score=124, Evalue=1e-28,
Organism=Homo sapiens, GI4503337, Length=260, Percent_Identity=29.2307692307692, Blast_Score=88, Evalue=9e-18,
Organism=Homo sapiens, GI215599015, Length=260, Percent_Identity=29.2307692307692, Blast_Score=88, Evalue=1e-17,
Organism=Escherichia coli, GI2367200, Length=227, Percent_Identity=37.8854625550661, Blast_Score=166, Evalue=2e-42,
Organism=Caenorhabditis elegans, GI17553978, Length=257, Percent_Identity=25.2918287937743, Blast_Score=82, Evalue=4e-16,
Organism=Saccharomyces cerevisiae, GI6323204, Length=297, Percent_Identity=29.2929292929293, Blast_Score=108, Evalue=1e-24,
Organism=Saccharomyces cerevisiae, GI6324037, Length=139, Percent_Identity=39.568345323741, Blast_Score=92, Evalue=1e-19,
Organism=Drosophila melanogaster, GI281364189, Length=259, Percent_Identity=29.7297297297297, Blast_Score=97, Evalue=1e-20,
Organism=Drosophila melanogaster, GI281364187, Length=259, Percent_Identity=29.7297297297297, Blast_Score=97, Evalue=1e-20,
Organism=Drosophila melanogaster, GI281364185, Length=259, Percent_Identity=29.7297297297297, Blast_Score=97, Evalue=1e-20,
Organism=Drosophila melanogaster, GI281364183, Length=259, Percent_Identity=29.7297297297297, Blast_Score=97, Evalue=1e-20,
Organism=Drosophila melanogaster, GI62471759, Length=259, Percent_Identity=29.7297297297297, Blast_Score=97, Evalue=1e-20,
Organism=Drosophila melanogaster, GI17975520, Length=259, Percent_Identity=29.7297297297297, Blast_Score=97, Evalue=1e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TRUB_STAA1 (A7X1Q5)

Other databases:

- EMBL:   AP009324
- RefSeq:   YP_001441851.1
- ProteinModelPortal:   A7X1Q5
- SMR:   A7X1Q5
- STRING:   A7X1Q5
- EnsemblBacteria:   EBSTAT00000005060
- GeneID:   5559657
- GenomeReviews:   AP009324_GR
- KEGG:   saw:SAHV_1261
- eggNOG:   COG0130
- GeneTree:   EBGT00050000024600
- HOGENOM:   HBG397258
- OMA:   LGCGAYV
- ProtClustDB:   PRK14123
- BioCyc:   SAUR418127:SAHV_1261-MONOMER
- HAMAP:   MF_01080
- InterPro:   IPR002501
- InterPro:   IPR020103
- InterPro:   IPR014780
- TIGRFAMs:   TIGR00431

Pfam domain/function: PF01509 TruB_N; SSF55120 PsdUridine_synth_cat_dom

EC number: 4.2.1.70

Molecular weight: Translated: 34606; Mature: 34606

Theoretical pI: Translated: 9.56; Mature: 9.56

Prosite motif: NA

Important sites: ACT_SITE 39-39

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYNGILPVYKERGLTSHDVVFKLRKILKTKKIGHTGTLDPEVAGVLPVCIGNATRVSDYV
CCCCCCCHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCCCHHHHHHH
MDMGKAYEATVSIGRSTTTEDQTGDTLETKGVHSADFNKDDIDRLLENFKGVIEQIPPMY
HHHCCHHHHHEECCCCCCCCCCCCCCHHCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCH
SSVKVNGKKLYEYARNNETVERPKRKVNIKDIGRISELDFKENECHFKIRVICGKGTYIR
HHEEECHHHHHHHHHCCCHHHHHHHCCCHHHCCCHHCCCCCCCCCEEEEEEEECCCCEEE
TLATDIGVKLGFPAHMSKLTRIESGGFVLKDSLTLEQIKELHEQDSLQNKLFPLEYGLKG
HHHHHHCEECCCHHHHHHHHEECCCCEEEECCCCHHHHHHHHHHHHHHHCCCCHHHCCCC
LPSIKIKDSHIKKRILNGQKFNKNEFDNKIKDQIVFIDDDSEKVLAIYMVHPTKESEIKP
CCCEEEEHHHHHHHHHCCCCCCCHHHCCHHHCEEEEEECCCCEEEEEEEECCCCCCCCCC
KKVFN
HHCCC
>Mature Secondary Structure
MYNGILPVYKERGLTSHDVVFKLRKILKTKKIGHTGTLDPEVAGVLPVCIGNATRVSDYV
CCCCCCCHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCCCHHHHHHH
MDMGKAYEATVSIGRSTTTEDQTGDTLETKGVHSADFNKDDIDRLLENFKGVIEQIPPMY
HHHCCHHHHHEECCCCCCCCCCCCCCHHCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCH
SSVKVNGKKLYEYARNNETVERPKRKVNIKDIGRISELDFKENECHFKIRVICGKGTYIR
HHEEECHHHHHHHHHCCCHHHHHHHCCCHHHCCCHHCCCCCCCCCEEEEEEEECCCCEEE
TLATDIGVKLGFPAHMSKLTRIESGGFVLKDSLTLEQIKELHEQDSLQNKLFPLEYGLKG
HHHHHHCEECCCHHHHHHHHEECCCCEEEECCCCHHHHHHHHHHHHHHHCCCCHHHCCCC
LPSIKIKDSHIKKRILNGQKFNKNEFDNKIKDQIVFIDDDSEKVLAIYMVHPTKESEIKP
CCCEEEEHHHHHHHHHCCCCCCCHHHCCHHHCEEEEEECCCCEEEEEEEECCCCCCCCCC
KKVFN
HHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: uracil; D-ribose 5-phosphate

Specific reaction: uracil + D-ribose 5-phosphate = pseudouridine 5'-phosphate + H2O

General reaction: addition of H2O; elimination of H2O; C-O bond cleavage [C]

Inhibitor: 1-(Tetrahydro-2-furanyl)-5-fluorouracil; 5-fluorouracil [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA