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The map label for this gene is rnhB

Identifier: 15926827

GI number: 15926827

Start: 1230383

End: 1231150

Strand: Direct

Name: rnhB

Synonym: SA1087

Alternate gene names: 15926827

Gene position: 1230383-1231150 (Clockwise)

Preceding gene: 15926826

Following gene: 15926828

Centisome position: 43.71

GC content: 35.03

Gene sequence:

>768_bases
ATGACGCTAACAATTAAAGAAGTTACGCAGTTGATTAATGCGGTTAATACAATAGAAGAATTAGAAAATCATGAATGCTT
TTTAGATGAGCGAAAAGGTGTTCAAAATGCCATAGCTAGGCGCAGAAAAGCGTTAGAAAAAGAACAAGCTTTAAAAGAAA
AGTATGTTGAAATGACTTACTTTGAAAATGAAATATTAAAAGAGCATCCTAATGCTATTATTTGTGGGATTGATGAAGTT
GGAAGAGGACCTTTAGCAGGTCCAGTCGTTGCATGCGCAACAATTTTAAATTCAAATCACAATTATTTGGGCCTTGATGA
CTCGAAAAAAGTACCTATTACGAAACGTCTAGAATTAAATGAAGCACTAAAAAATGAAGTTACTGCTTTTGCATATGGTA
TCGCGACAGCTGAAGAAATAGATGAATTTAATATTTATAAAGCTACTCAAATCGCCATGCAGCGAGCTATTGATGGATTA
TCAGTACAACCAACGCATTTATTGATAGACGCGATGACGCTTGATAATGCACTGCCTCAAGTATCTTTAATCAAGGGTGA
TGCAAGAAGTGTGTCCATTGCAGCTGCAAGTATCATGGCGAAGGTTTTTCGTGATGATTATATGACACAGTTATCTAAAG
ATTATCCTGAATACGGTTTTGAAAAAAACGCGGGTTATGGTACCAAACAACATTTACTAGCAATCGATGATATTGGCATT
ATGAAAGAGCATAGAAAAAGCTTTGAACCTATAAAATCGTTACTGTAA

Upstream 100 bases:

>100_bases
TACGAAGCAGTCATTGAACTGATTATTTATGATATTCGAAATGCTAAAATAGGAAATTATTGTTTTGATATTTTTAAAGA
TATGACTGAGGAATTAGCAA

Downstream 100 bases:

>100_bases
ATCGTAAAATTCAATTAAATGATAAAAAAGTAGCATTTAAGTTTACAATAGCGCTTACATTTTTTATAATTGTAAGGGAA
CTTAACCTAAGTAACAGGAG

Product: ribonuclease HII

Products: NA

Alternate protein names: RNase HII

Number of amino acids: Translated: 255; Mature: 254

Protein sequence:

>255_residues
MTLTIKEVTQLINAVNTIEELENHECFLDERKGVQNAIARRRKALEKEQALKEKYVEMTYFENEILKEHPNAIICGIDEV
GRGPLAGPVVACATILNSNHNYLGLDDSKKVPITKRLELNEALKNEVTAFAYGIATAEEIDEFNIYKATQIAMQRAIDGL
SVQPTHLLIDAMTLDNALPQVSLIKGDARSVSIAAASIMAKVFRDDYMTQLSKDYPEYGFEKNAGYGTKQHLLAIDDIGI
MKEHRKSFEPIKSLL

Sequences:

>Translated_255_residues
MTLTIKEVTQLINAVNTIEELENHECFLDERKGVQNAIARRRKALEKEQALKEKYVEMTYFENEILKEHPNAIICGIDEV
GRGPLAGPVVACATILNSNHNYLGLDDSKKVPITKRLELNEALKNEVTAFAYGIATAEEIDEFNIYKATQIAMQRAIDGL
SVQPTHLLIDAMTLDNALPQVSLIKGDARSVSIAAASIMAKVFRDDYMTQLSKDYPEYGFEKNAGYGTKQHLLAIDDIGI
MKEHRKSFEPIKSLL
>Mature_254_residues
TLTIKEVTQLINAVNTIEELENHECFLDERKGVQNAIARRRKALEKEQALKEKYVEMTYFENEILKEHPNAIICGIDEVG
RGPLAGPVVACATILNSNHNYLGLDDSKKVPITKRLELNEALKNEVTAFAYGIATAEEIDEFNIYKATQIAMQRAIDGLS
VQPTHLLIDAMTLDNALPQVSLIKGDARSVSIAAASIMAKVFRDDYMTQLSKDYPEYGFEKNAGYGTKQHLLAIDDIGIM
KEHRKSFEPIKSLL

Specific function: Endonuclease that specifically degrades the RNA of RNA- DNA hybrids

COG id: COG0164

COG function: function code L; Ribonuclease HII

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNase HII family

Homologues:

Organism=Escherichia coli, GI1786380, Length=184, Percent_Identity=44.0217391304348, Blast_Score=158, Evalue=3e-40,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RNH2_STAA1 (A7X1L7)

Other databases:

- EMBL:   AP009324
- RefSeq:   YP_001441824.1
- ProteinModelPortal:   A7X1L7
- SMR:   A7X1L7
- STRING:   A7X1L7
- EnsemblBacteria:   EBSTAT00000003339
- GeneID:   5560779
- GenomeReviews:   AP009324_GR
- KEGG:   saw:SAHV_1234
- eggNOG:   COG0164
- GeneTree:   EBGT00050000024986
- HOGENOM:   HBG584843
- OMA:   RLGPTPI
- ProtClustDB:   PRK00015
- BioCyc:   SAUR418127:SAHV_1234-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00052_B
- InterPro:   IPR022898
- InterPro:   IPR001352
- InterPro:   IPR012337
- PANTHER:   PTHR10954

Pfam domain/function: PF01351 RNase_HII; SSF53098 RNaseH_fold

EC number: =3.1.26.4

Molecular weight: Translated: 28527; Mature: 28396

Theoretical pI: Translated: 5.23; Mature: 5.23

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTLTIKEVTQLINAVNTIEELENHECFLDERKGVQNAIARRRKALEKEQALKEKYVEMTY
CEEEHHHHHHHHHHHHHHHHHHCCCHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
FENEILKEHPNAIICGIDEVGRGPLAGPVVACATILNSNHNYLGLDDSKKVPITKRLELN
HHHHHHHHCCCEEEECCHHHCCCCCCHHHHHHHHHHCCCCCEECCCCCCCCCCHHHHHHH
EALKNEVTAFAYGIATAEEIDEFNIYKATQIAMQRAIDGLSVQPTHLLIDAMTLDNALPQ
HHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCC
VSLIKGDARSVSIAAASIMAKVFRDDYMTQLSKDYPEYGFEKNAGYGTKQHLLAIDDIGI
EEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHEEEEHHHHH
MKEHRKSFEPIKSLL
HHHHHHHHHHHHHCC
>Mature Secondary Structure 
TLTIKEVTQLINAVNTIEELENHECFLDERKGVQNAIARRRKALEKEQALKEKYVEMTY
EEEHHHHHHHHHHHHHHHHHHCCCHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
FENEILKEHPNAIICGIDEVGRGPLAGPVVACATILNSNHNYLGLDDSKKVPITKRLELN
HHHHHHHHCCCEEEECCHHHCCCCCCHHHHHHHHHHCCCCCEECCCCCCCCCCHHHHHHH
EALKNEVTAFAYGIATAEEIDEFNIYKATQIAMQRAIDGLSVQPTHLLIDAMTLDNALPQ
HHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCC
VSLIKGDARSVSIAAASIMAKVFRDDYMTQLSKDYPEYGFEKNAGYGTKQHLLAIDDIGI
EEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHEEEEHHHHH
MKEHRKSFEPIKSLL
HHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA