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The map label for this gene is menD

Identifier: 15926630

GI number: 15926630

Start: 1016502

End: 1018175

Strand: Direct

Name: menD

Synonym: SA0896

Alternate gene names: 15926630

Gene position: 1016502-1018175 (Clockwise)

Preceding gene: 15926629

Following gene: 15926631

Centisome position: 36.11

GC content: 33.69

Gene sequence:

>1674_bases
ATGGGAAATCATAAAGCAGCTTTAACGAAGCAAGTTTTTACATTTGCATCTGAGTTATATGCATACGGCGTAAGGGAAGT
AGTTATCAGTCCGGGATCACGCTCAACGCCACTTGCACTTGCATTTGAAGCACATCCAAATATTAAAACATGGATACACC
CCGATGAGCGAAGTGCAGCATTTTTTGCAGTTGGGTTAATTAAAGGCAGTGAAAGACCTGTCGCTATATTATGTACGTCA
GGTACAGCAGCAGCGAATTATACGCCTGCAATTGCTGAAAGCCAAATTAGTAGAATTCCATTAATCGTTTTAACAAGTGA
CCGTCCGCATGAATTAAGAAGTGTAGGCGCACCACAAGCGATTAATCAAGTAAATATGTTTAATAATTATGTAAGTTATG
AGTTCGATATGCCTATTGCGGATGATAGTAAAGAGACCATTAATGCAATTTATTATCAAATGCAAATTGCTAGTCAATAT
TTATATGGACCACATAAAGGGCCAATTCATTTTAACTTGCCATTTAGAGATCCGTTAACACCTGATTTGAATGCAACAGA
ATTGTTAACTTCTGAGATGAAGATTTTACCGCACTATCAAAAAAGTATAGATGCATCGGCATTAAGACACATTTTAAATA
AGAAAAAAGGTTTAATTATTGTAGGGGATATGCAGCACCAAGAAGTTGATCAAATACTAACGTATTCAACGATATATGAT
TTGCCTATTTTAGCTGATCCTTTAAGTCATTTAAGAAAATTTGATCATCCGAATGTTATCTGTACATATGATTTGCTGTT
TAGAAGCGGCTTAGACTTAAATGTGGATTTCGTAATTCGTGTTGGGAAACCAGTGATTTCTAAAAAGTTGAATCAATGGT
TAAAGAAAACTGATGCATTTCAAATATTAGTGCAAAACAATGATAAGATTGATGTCTTTCCTATAGCGCCAGATATTTCA
TATGAGATTTCTGCAAATGATTTCTTTAGATCATTAATGGAAGACACGACCATCAATCGCGTAAGTTGGTTAGAAAAATG
GCAATGCTTAGAGAAAAAAGGGCGTAAAGAAATTAAATGTTATTTGGAACAAGCTACAGATGAGAGTGCATTCGTTGGTG
AATTGATTAAGAAAACATCTGAAAAAGATGCATTATTTATTAGTAATAGTATGCCTATCAGAGATGTAGATAACTTGTTA
TTGAATAAAAATATAGATGTCTATGCGAATCGTGGTGCGAATGGTATTGATGGTATCGTTTCAACTGCACTGGGTATGGC
TGTGCATAAACGAATAACATTATTGATAGGTGATTTGTCATTTTATCATGATATGAATGGACTATTAATGTCAAAATTAA
ATAATATTCAGATGAATATTGTATTATTGAACAACGATGGTGGCGGTATTTTTTCATATTTACCACAAAAAGAAAGTGCA
ACTGACTATTTTGAACGGTTGTTTGGCACACCGACGGGATTGGATTTCGAGTATACAGCTAAGTTATATCAATTCGATTT
TAAACGTTTTAACAGTGTTTCAGAATTTAAAAATGCCACATTGTTATCTGAAACTTCGACGATTTATGAATTGATAACGA
ATCGCGAAGATAACTTTAAACAGCATCAAATTTTATATCAGAAATTGAGTGAAATGATTCATGACACATTATAA

Upstream 100 bases:

>100_bases
GCTGGGTGTGGCATTGTTAAAGATTCTGATCCAGATAGTGAATTGGCAGAAACGAACCTTAAGTTCACACCTATGATGAA
TGCATTAGGAGTCGATATGA

Downstream 100 bases:

>100_bases
ATACTATGAAGCAAACGTTGAGACCAATCAAGTTTTAGTATTACTGCATGGTTTTCTTAGCGACAGTCGTACTTATTATA
ATCACATCGATAAATATACT

Product: menaquinone biosynthesis protein

Products: NA

Alternate protein names: SEPHCHC synthase; Menaquinone biosynthesis protein menD

Number of amino acids: Translated: 557; Mature: 556

Protein sequence:

>557_residues
MGNHKAALTKQVFTFASELYAYGVREVVISPGSRSTPLALAFEAHPNIKTWIHPDERSAAFFAVGLIKGSERPVAILCTS
GTAAANYTPAIAESQISRIPLIVLTSDRPHELRSVGAPQAINQVNMFNNYVSYEFDMPIADDSKETINAIYYQMQIASQY
LYGPHKGPIHFNLPFRDPLTPDLNATELLTSEMKILPHYQKSIDASALRHILNKKKGLIIVGDMQHQEVDQILTYSTIYD
LPILADPLSHLRKFDHPNVICTYDLLFRSGLDLNVDFVIRVGKPVISKKLNQWLKKTDAFQILVQNNDKIDVFPIAPDIS
YEISANDFFRSLMEDTTINRVSWLEKWQCLEKKGRKEIKCYLEQATDESAFVGELIKKTSEKDALFISNSMPIRDVDNLL
LNKNIDVYANRGANGIDGIVSTALGMAVHKRITLLIGDLSFYHDMNGLLMSKLNNIQMNIVLLNNDGGGIFSYLPQKESA
TDYFERLFGTPTGLDFEYTAKLYQFDFKRFNSVSEFKNATLLSETSTIYELITNREDNFKQHQILYQKLSEMIHDTL

Sequences:

>Translated_557_residues
MGNHKAALTKQVFTFASELYAYGVREVVISPGSRSTPLALAFEAHPNIKTWIHPDERSAAFFAVGLIKGSERPVAILCTS
GTAAANYTPAIAESQISRIPLIVLTSDRPHELRSVGAPQAINQVNMFNNYVSYEFDMPIADDSKETINAIYYQMQIASQY
LYGPHKGPIHFNLPFRDPLTPDLNATELLTSEMKILPHYQKSIDASALRHILNKKKGLIIVGDMQHQEVDQILTYSTIYD
LPILADPLSHLRKFDHPNVICTYDLLFRSGLDLNVDFVIRVGKPVISKKLNQWLKKTDAFQILVQNNDKIDVFPIAPDIS
YEISANDFFRSLMEDTTINRVSWLEKWQCLEKKGRKEIKCYLEQATDESAFVGELIKKTSEKDALFISNSMPIRDVDNLL
LNKNIDVYANRGANGIDGIVSTALGMAVHKRITLLIGDLSFYHDMNGLLMSKLNNIQMNIVLLNNDGGGIFSYLPQKESA
TDYFERLFGTPTGLDFEYTAKLYQFDFKRFNSVSEFKNATLLSETSTIYELITNREDNFKQHQILYQKLSEMIHDTL
>Mature_556_residues
GNHKAALTKQVFTFASELYAYGVREVVISPGSRSTPLALAFEAHPNIKTWIHPDERSAAFFAVGLIKGSERPVAILCTSG
TAAANYTPAIAESQISRIPLIVLTSDRPHELRSVGAPQAINQVNMFNNYVSYEFDMPIADDSKETINAIYYQMQIASQYL
YGPHKGPIHFNLPFRDPLTPDLNATELLTSEMKILPHYQKSIDASALRHILNKKKGLIIVGDMQHQEVDQILTYSTIYDL
PILADPLSHLRKFDHPNVICTYDLLFRSGLDLNVDFVIRVGKPVISKKLNQWLKKTDAFQILVQNNDKIDVFPIAPDISY
EISANDFFRSLMEDTTINRVSWLEKWQCLEKKGRKEIKCYLEQATDESAFVGELIKKTSEKDALFISNSMPIRDVDNLLL
NKNIDVYANRGANGIDGIVSTALGMAVHKRITLLIGDLSFYHDMNGLLMSKLNNIQMNIVLLNNDGGGIFSYLPQKESAT
DYFERLFGTPTGLDFEYTAKLYQFDFKRFNSVSEFKNATLLSETSTIYELITNREDNFKQHQILYQKLSEMIHDTL

Specific function: Catalyzes the thiamine diphosphate-dependent decarboxylation of 2-oxoglutarate and the subsequent addition of the resulting succinic semialdehyde-thiamine pyrophosphate anion to isochorismate to yield 2-succinyl-5-enolpyruvyl-6-hydroxy-3- cyclohexene-1-ca

COG id: COG1165

COG function: function code H; 2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family. MenD subfamily

Homologues:

Organism=Escherichia coli, GI1788599, Length=572, Percent_Identity=29.5454545454545, Blast_Score=214, Evalue=9e-57,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MEND_STAA1 (A7X0S7)

Other databases:

- EMBL:   AP009324
- RefSeq:   YP_001441626.1
- ProteinModelPortal:   A7X0S7
- STRING:   A7X0S7
- EnsemblBacteria:   EBSTAT00000004732
- GeneID:   5559196
- GenomeReviews:   AP009324_GR
- KEGG:   saw:SAHV_1036
- eggNOG:   COG1165
- GeneTree:   EBGT00050000023981
- HOGENOM:   HBG634405
- OMA:   FYHDLNG
- ProtClustDB:   CLSK885095
- BioCyc:   SAUR418127:SAHV_1036-MONOMER
- HAMAP:   MF_01659
- InterPro:   IPR004433
- InterPro:   IPR012001
- InterPro:   IPR011766
- TIGRFAMs:   TIGR00173

Pfam domain/function: PF02775 TPP_enzyme_C; PF02776 TPP_enzyme_N

EC number: =2.2.1.9

Molecular weight: Translated: 63092; Mature: 62961

Theoretical pI: Translated: 6.35; Mature: 6.35

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGNHKAALTKQVFTFASELYAYGVREVVISPGSRSTPLALAFEAHPNIKTWIHPDERSAA
CCCCHHHHHHHHHHHHHHHHHHCCEEEEECCCCCCCCEEEEEECCCCCEEEECCCCCCCE
FFAVGLIKGSERPVAILCTSGTAAANYTPAIAESQISRIPLIVLTSDRPHELRSVGAPQA
EEEEEEEECCCCCEEEEEECCCCCCCCCCHHHHHHHHHCCEEEEECCCCHHHHHCCCCHH
INQVNMFNNYVSYEFDMPIADDSKETINAIYYQMQIASQYLYGPHKGPIHFNLPFRDPLT
HHHHHHHHCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCCCC
PDLNATELLTSEMKILPHYQKSIDASALRHILNKKKGLIIVGDMQHQEVDQILTYSTIYD
CCCCHHHHHHHHHHHCCHHHHHCCHHHHHHHHCCCCCEEEEECCCHHHHHHHHHHHHHHC
LPILADPLSHLRKFDHPNVICTYDLLFRSGLDLNVDFVIRVGKPVISKKLNQWLKKTDAF
CCHHHHHHHHHHHCCCCCEEEEEEHHHHCCCCCCEEEEEECCCHHHHHHHHHHHHCCCEE
QILVQNNDKIDVFPIAPDISYEISANDFFRSLMEDTTINRVSWLEKWQCLEKKGRKEIKC
EEEEECCCEEEEEECCCCCEEEECHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCHHHHHH
YLEQATDESAFVGELIKKTSEKDALFISNSMPIRDVDNLLLNKNIDVYANRGANGIDGIV
HHHHCCCCHHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHCCCCEEEECCCCCCHHHHH
STALGMAVHKRITLLIGDLSFYHDMNGLLMSKLNNIQMNIVLLNNDGGGIFSYLPQKESA
HHHHHHHHHHEEEEEEECHHHHHHHHHHHHHHCCCEEEEEEEEECCCCCEEEECCCCCCH
TDYFERLFGTPTGLDFEYTAKLYQFDFKRFNSVSEFKNATLLSETSTIYELITNREDNFK
HHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCHHHHCCCEEHHHHHHHHHHHHCCCCCHH
QHQILYQKLSEMIHDTL
HHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
GNHKAALTKQVFTFASELYAYGVREVVISPGSRSTPLALAFEAHPNIKTWIHPDERSAA
CCCHHHHHHHHHHHHHHHHHHCCEEEEECCCCCCCCEEEEEECCCCCEEEECCCCCCCE
FFAVGLIKGSERPVAILCTSGTAAANYTPAIAESQISRIPLIVLTSDRPHELRSVGAPQA
EEEEEEEECCCCCEEEEEECCCCCCCCCCHHHHHHHHHCCEEEEECCCCHHHHHCCCCHH
INQVNMFNNYVSYEFDMPIADDSKETINAIYYQMQIASQYLYGPHKGPIHFNLPFRDPLT
HHHHHHHHCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCCCC
PDLNATELLTSEMKILPHYQKSIDASALRHILNKKKGLIIVGDMQHQEVDQILTYSTIYD
CCCCHHHHHHHHHHHCCHHHHHCCHHHHHHHHCCCCCEEEEECCCHHHHHHHHHHHHHHC
LPILADPLSHLRKFDHPNVICTYDLLFRSGLDLNVDFVIRVGKPVISKKLNQWLKKTDAF
CCHHHHHHHHHHHCCCCCEEEEEEHHHHCCCCCCEEEEEECCCHHHHHHHHHHHHCCCEE
QILVQNNDKIDVFPIAPDISYEISANDFFRSLMEDTTINRVSWLEKWQCLEKKGRKEIKC
EEEEECCCEEEEEECCCCCEEEECHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCHHHHHH
YLEQATDESAFVGELIKKTSEKDALFISNSMPIRDVDNLLLNKNIDVYANRGANGIDGIV
HHHHCCCCHHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHCCCCEEEECCCCCCHHHHH
STALGMAVHKRITLLIGDLSFYHDMNGLLMSKLNNIQMNIVLLNNDGGGIFSYLPQKESA
HHHHHHHHHHEEEEEEECHHHHHHHHHHHHHHCCCEEEEEEEEECCCCCEEEECCCCCCH
TDYFERLFGTPTGLDFEYTAKLYQFDFKRFNSVSEFKNATLLSETSTIYELITNREDNFK
HHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCHHHHCCCEEHHHHHHHHHHHHCCCCCHH
QHQILYQKLSEMIHDTL
HHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA