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The map label for this gene is menF [H]

Identifier: 15926629

GI number: 15926629

Start: 1015154

End: 1016515

Strand: Direct

Name: menF [H]

Synonym: SA0895

Alternate gene names: 15926629

Gene position: 1015154-1016515 (Clockwise)

Preceding gene: 15926627

Following gene: 15926630

Centisome position: 36.06

GC content: 31.94

Gene sequence:

>1362_bases
ATGGCTACGGGCGTATTAGAGGACGATATTGTCAAAGAGATATATGGCAGCTCAAAGGAATGGGTTTCAGTTGAAGTGAA
ATTATCACAGTCACTAGACCCGAGCACATTATTTCATCTCACTGACAATGAGGCAGGAGATCGCTTTTATATGCGTTTGA
ATGATAATCGAACGTCATATTTTGGCTACAAAGCAATTCAATTATTCAAAAATAATTCTAAAAATAAACAATCTATTTTT
AAAGACTGGGAAAAATTAAAACATAACATCACATTTATACATCCGCAATCTGAGAAACATCATCTTCGAGTTGTTGGAGG
GTTTCAATTTTCAAGTCATAAATCAGATGATGAATGGCGAGAGTTTGGACTAAATCATTTTGTATTACCTGAAGTTTTAA
TTTCAACTGATAATAATGGGACATTTTTAACTTATACAGTTAAAAGGGAAAGTTTTACTGTTGAGGCATTGAACGATTTA
ATGGATTTGTTCAACAATATATCGGATATAGATGTGGACGAGCAAATTGGGGAAATTACTAGAAATGAAGATATTTATAA
AGATGACTGGCGTCAACTTGTAGTAGAAGCTATAGAATCTATTAATAATGAAGAAAAAATTGTACTAGCACGTAGACGGT
TAATAAAGTTCGATAAAGATATCAGTATTCCATATATTCTAAAGCAAGCATATTCTAAAGAAAAAAACAGTTATATATTC
TTGTTAGAATCACAAGATTCTATTTTCTTTTCACAAACACCTGAACAATTAATAAAGGTCAATAATAAAATACTATCGAC
TAAAGCTGTAGCAGGTACAATTAAACGTTCACAAGATGAGGACGAAGATACAAAAAATGTTGAAGCATTTTTAAAAGATA
ATAAAAACTTAATCGAACATCGATTTGTTGTTGACAGTATTTTACATGATATTAAACCTTATATCACTGAATTACATTAT
GATAAGACGCCTAAAATTCTAAAAAATGATCATTTATATCACTTGTACACTGAAATAAAGGCGCCACTGAAGGATGATTC
GTATATTAGTTTAATTGATCATTTACATCCAACACCTGCTTTAGGTGGCTATCCAAAAGAATTTGCGATGGATTTTATTG
AACAGAAAGAATTTGGTACACGAGGATTATATGGTGCGCCGGTTGGCTATATAGATATATATGATGATTGTGAATTTATT
GTTGCAATTCGTTCGATGCTTATTAAGAAAGCACAAGCAACTTTATTTGCTGGGTGTGGCATTGTTAAAGATTCTGATCC
AGATAGTGAATTGGCAGAAACGAACCTTAAGTTCACACCTATGATGAATGCATTAGGAGTCGATATGAATGGGAAATCAT
AA

Upstream 100 bases:

>100_bases
AAAATTGCAAGATAAGTTTAAGCAATTCTTGTAACACAATTATCCAAAAATGATACAATATAATAATGAGTGAAAAAAGA
CGAAGAAAGAAGTGAAATGG

Downstream 100 bases:

>100_bases
AGCAGCTTTAACGAAGCAAGTTTTTACATTTGCATCTGAGTTATATGCATACGGCGTAAGGGAAGTAGTTATCAGTCCGG
GATCACGCTCAACGCCACTT

Product: hypothetical protein

Products: NA

Alternate protein names: Isochorismate mutase [H]

Number of amino acids: Translated: 453; Mature: 452

Protein sequence:

>453_residues
MATGVLEDDIVKEIYGSSKEWVSVEVKLSQSLDPSTLFHLTDNEAGDRFYMRLNDNRTSYFGYKAIQLFKNNSKNKQSIF
KDWEKLKHNITFIHPQSEKHHLRVVGGFQFSSHKSDDEWREFGLNHFVLPEVLISTDNNGTFLTYTVKRESFTVEALNDL
MDLFNNISDIDVDEQIGEITRNEDIYKDDWRQLVVEAIESINNEEKIVLARRRLIKFDKDISIPYILKQAYSKEKNSYIF
LLESQDSIFFSQTPEQLIKVNNKILSTKAVAGTIKRSQDEDEDTKNVEAFLKDNKNLIEHRFVVDSILHDIKPYITELHY
DKTPKILKNDHLYHLYTEIKAPLKDDSYISLIDHLHPTPALGGYPKEFAMDFIEQKEFGTRGLYGAPVGYIDIYDDCEFI
VAIRSMLIKKAQATLFAGCGIVKDSDPDSELAETNLKFTPMMNALGVDMNGKS

Sequences:

>Translated_453_residues
MATGVLEDDIVKEIYGSSKEWVSVEVKLSQSLDPSTLFHLTDNEAGDRFYMRLNDNRTSYFGYKAIQLFKNNSKNKQSIF
KDWEKLKHNITFIHPQSEKHHLRVVGGFQFSSHKSDDEWREFGLNHFVLPEVLISTDNNGTFLTYTVKRESFTVEALNDL
MDLFNNISDIDVDEQIGEITRNEDIYKDDWRQLVVEAIESINNEEKIVLARRRLIKFDKDISIPYILKQAYSKEKNSYIF
LLESQDSIFFSQTPEQLIKVNNKILSTKAVAGTIKRSQDEDEDTKNVEAFLKDNKNLIEHRFVVDSILHDIKPYITELHY
DKTPKILKNDHLYHLYTEIKAPLKDDSYISLIDHLHPTPALGGYPKEFAMDFIEQKEFGTRGLYGAPVGYIDIYDDCEFI
VAIRSMLIKKAQATLFAGCGIVKDSDPDSELAETNLKFTPMMNALGVDMNGKS
>Mature_452_residues
ATGVLEDDIVKEIYGSSKEWVSVEVKLSQSLDPSTLFHLTDNEAGDRFYMRLNDNRTSYFGYKAIQLFKNNSKNKQSIFK
DWEKLKHNITFIHPQSEKHHLRVVGGFQFSSHKSDDEWREFGLNHFVLPEVLISTDNNGTFLTYTVKRESFTVEALNDLM
DLFNNISDIDVDEQIGEITRNEDIYKDDWRQLVVEAIESINNEEKIVLARRRLIKFDKDISIPYILKQAYSKEKNSYIFL
LESQDSIFFSQTPEQLIKVNNKILSTKAVAGTIKRSQDEDEDTKNVEAFLKDNKNLIEHRFVVDSILHDIKPYITELHYD
KTPKILKNDHLYHLYTEIKAPLKDDSYISLIDHLHPTPALGGYPKEFAMDFIEQKEFGTRGLYGAPVGYIDIYDDCEFIV
AIRSMLIKKAQATLFAGCGIVKDSDPDSELAETNLKFTPMMNALGVDMNGKS

Specific function: Siderophore biosynthesis; enterobactin biosynthesis. [C]

COG id: COG1169

COG function: function code HQ; Isochorismate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the isochorismate synthase family [H]

Homologues:

Organism=Escherichia coli, GI1786809, Length=254, Percent_Identity=31.1023622047244, Blast_Score=127, Evalue=2e-30,
Organism=Escherichia coli, GI87082077, Length=265, Percent_Identity=29.0566037735849, Blast_Score=112, Evalue=3e-26,
Organism=Escherichia coli, GI1787518, Length=258, Percent_Identity=28.6821705426357, Blast_Score=96, Evalue=6e-21,
Organism=Escherichia coli, GI1788114, Length=199, Percent_Identity=28.1407035175879, Blast_Score=77, Evalue=2e-15,
Organism=Saccharomyces cerevisiae, GI6320935, Length=209, Percent_Identity=31.1004784688995, Blast_Score=82, Evalue=1e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005801
- InterPro:   IPR015890
- InterPro:   IPR004561 [H]

Pfam domain/function: PF00425 Chorismate_bind [H]

EC number: =5.4.4.2 [H]

Molecular weight: Translated: 52246; Mature: 52114

Theoretical pI: Translated: 5.10; Mature: 5.10

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATGVLEDDIVKEIYGSSKEWVSVEVKLSQSLDPSTLFHLTDNEAGDRFYMRLNDNRTSY
CCCCCCHHHHHHHHHCCCCCEEEEEEEECCCCCCCEEEEEECCCCCCEEEEEECCCCCEE
FGYKAIQLFKNNSKNKQSIFKDWEKLKHNITFIHPQSEKHHLRVVGGFQFSSHKSDDEWR
HHHHHHHEECCCCCCHHHHHHHHHHHHCCEEEECCCCCCCEEEEEEEEEECCCCCCHHHH
EFGLNHFVLPEVLISTDNNGTFLTYTVKRESFTVEALNDLMDLFNNISDIDVDEQIGEIT
HHCCCCEECCEEEEEECCCCEEEEEEEECCCEEHHHHHHHHHHHCCCCCCCHHHHHHHHH
RNEDIYKDDWRQLVVEAIESINNEEKIVLARRRLIKFDKDISIPYILKQAYSKEKNSYIF
CCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCCCEEE
LLESQDSIFFSQTPEQLIKVNNKILSTKAVAGTIKRSQDEDEDTKNVEAFLKDNKNLIEH
EEECCCCEEECCCHHHHHHHCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCHHHHHH
RFVVDSILHDIKPYITELHYDKTPKILKNDHLYHLYTEIKAPLKDDSYISLIDHLHPTPA
HHHHHHHHHHHHHHHHHHCCCCCCCHHCCCCEEEEEEHHCCCCCCCCHHHHHHHCCCCCC
LGGYPKEFAMDFIEQKEFGTRGLYGAPVGYIDIYDDCEFIVAIRSMLIKKAQATLFAGCG
CCCCCHHHHHHHHHHHHCCCCCCCCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHCCC
IVKDSDPDSELAETNLKFTPMMNALGVDMNGKS
CCCCCCCCHHHHHCCCEEEECHHHHCCCCCCCC
>Mature Secondary Structure 
ATGVLEDDIVKEIYGSSKEWVSVEVKLSQSLDPSTLFHLTDNEAGDRFYMRLNDNRTSY
CCCCCHHHHHHHHHCCCCCEEEEEEEECCCCCCCEEEEEECCCCCCEEEEEECCCCCEE
FGYKAIQLFKNNSKNKQSIFKDWEKLKHNITFIHPQSEKHHLRVVGGFQFSSHKSDDEWR
HHHHHHHEECCCCCCHHHHHHHHHHHHCCEEEECCCCCCCEEEEEEEEEECCCCCCHHHH
EFGLNHFVLPEVLISTDNNGTFLTYTVKRESFTVEALNDLMDLFNNISDIDVDEQIGEIT
HHCCCCEECCEEEEEECCCCEEEEEEEECCCEEHHHHHHHHHHHCCCCCCCHHHHHHHHH
RNEDIYKDDWRQLVVEAIESINNEEKIVLARRRLIKFDKDISIPYILKQAYSKEKNSYIF
CCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCCCEEE
LLESQDSIFFSQTPEQLIKVNNKILSTKAVAGTIKRSQDEDEDTKNVEAFLKDNKNLIEH
EEECCCCEEECCCHHHHHHHCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCHHHHHH
RFVVDSILHDIKPYITELHYDKTPKILKNDHLYHLYTEIKAPLKDDSYISLIDHLHPTPA
HHHHHHHHHHHHHHHHHHCCCCCCCHHCCCCEEEEEEHHCCCCCCCCHHHHHHHCCCCCC
LGGYPKEFAMDFIEQKEFGTRGLYGAPVGYIDIYDDCEFIVAIRSMLIKKAQATLFAGCG
CCCCCHHHHHHHHHHHHCCCCCCCCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHCCC
IVKDSDPDSELAETNLKFTPMMNALGVDMNGKS
CCCCCCCCHHHHHCCCEEEECHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1629163; 8566759; 9387221; 9384377; 3131310 [H]