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The map label for this gene is gspE [C]

Identifier: 15921686

GI number: 15921686

Start: 1402369

End: 1403793

Strand: Direct

Name: gspE [C]

Synonym: ST1397

Alternate gene names: 15921686

Gene position: 1402369-1403793 (Clockwise)

Preceding gene: 15921685

Following gene: 15921687

Centisome position: 52.04

GC content: 28.84

Gene sequence:

>1425_bases
ATGACAACTATTCTTCATGAGTATTATGTAGGTCCAGCTAAAATAAGAATATTACGTGATGATAATGGAATTTGTAAATA
CATTGTTGAAGAACCACAACTAACAGAATACGAAGAAGAAATAAAGAATTCCATATTATCTGAAATTATGTATACAAATT
TAAAAAACATAGAAGATTTTGTAATTAGTAAGTTAAAAGATAAAGGATTTAAAGATGATGTAATTGAAAAAATTCTTTAT
CATGTTAAAAAAAGCATGTTATATGATAATATAACTCCATTAATGTTAGACCAAGAAATTGAAGAAATTGAATGTAGAGG
TTTTGGTTATCCTATAACTGTCATTCACAGATCTTTTTCGGAATGTATTAGACTTTTCACGAATATAATTCCAAAAAACG
AGGATGATATTATAAAAATTGTTGAGAAAATGGCAAATAAGGCCAATAAAAGTATTAATATAGCTAAACCTTATGTTGAG
TTTTCACTCCCAGAAGGACATAGAGTAGCTGCTACTTTAGGCAGTGAAATATCATTACCTGGTTCTACATTCGATATACG
TAAGTTTCCGTCTAAACCTCTCAGTATTATCCAGATGATCACTAATGGTATGTTAAATGAGCTAATAGCATCTTATTTAT
GGTTCATAATGGAATATAAACCATTTATAATGATCTTGGGACCTACTGGTTCTGGTAAGACTTCTCTCTTAACAGCTATT
CTTAATTTAATTAATCCAAGCTATAAGATTTTAACTATTGAAGATACTCCAGAAATAAATATAACCAGTGACAATTGGGT
TAGGTTTATAAGTAGATCAACTTTGGCTGGTAACTATGATGTAACTTTAAGTGATTTAGCTAAATTAGCTCTACGATATA
GGCCCGATTATTTAGTGGTTGGAGAAGTTAGAGGAAAAGAGATAGAAGCTCTAATTCATGCTGCAGCATCTGGCCATGGT
TCTTTAACTACTTTTCATGGTTCAAGACCTATAGACGCTATTACTAGAATAACTGATTTACTATCTTCTGATTTGTCTAA
ACTTTTTTTGCAAACTATATGGTCCTTCATAATAGTAAGTAGAAGAAAAGAAGGAAACAAAAGTATTCGTAGTATAATCG
GAATATATGAGACTTTAATAGATAAAAATAAAATTAAATTTAAAAAAGTAATTGAATGGTCGTTTATAAAAAATGAATTT
ATTCCTCTAGATATAAATTCTTTATCAAAGAGATCATATAGACTAAAATGGATTAGTAAAGTTTATGGGTTATCCATTGA
AGAGATAAGACAAGAAATAGAAAGAAGGATGAATTTCCTTACTACTTTAAAAAACGAGAAAGTTATTGATTTTGTTGATG
TTTCTTATAAGATAAGAAAGTTTTATGAAGTAGGTGAGGCTAGTGCTAAGAATGTTGCTCAATAA

Upstream 100 bases:

>100_bases
CAGTGGTTAGCGCGGTAAAATTAAAAGAAGAGCTAGCTACTTTTGGTCTTTTATAATATTTTCACTTTGCTCAGAGTTCT
TTTTTATTTACCTAAATAAT

Downstream 100 bases:

>100_bases
GCTCAATAAAATTTCTCCATGTAATGTTTTTAATCAGAAAATTAAGGAGTATATTGAATATTATGGCGATGATTATAACA
AAATCTGTAGTAAGTATCAT

Product: virB11 protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 474; Mature: 473

Protein sequence:

>474_residues
MTTILHEYYVGPAKIRILRDDNGICKYIVEEPQLTEYEEEIKNSILSEIMYTNLKNIEDFVISKLKDKGFKDDVIEKILY
HVKKSMLYDNITPLMLDQEIEEIECRGFGYPITVIHRSFSECIRLFTNIIPKNEDDIIKIVEKMANKANKSINIAKPYVE
FSLPEGHRVAATLGSEISLPGSTFDIRKFPSKPLSIIQMITNGMLNELIASYLWFIMEYKPFIMILGPTGSGKTSLLTAI
LNLINPSYKILTIEDTPEINITSDNWVRFISRSTLAGNYDVTLSDLAKLALRYRPDYLVVGEVRGKEIEALIHAAASGHG
SLTTFHGSRPIDAITRITDLLSSDLSKLFLQTIWSFIIVSRRKEGNKSIRSIIGIYETLIDKNKIKFKKVIEWSFIKNEF
IPLDINSLSKRSYRLKWISKVYGLSIEEIRQEIERRMNFLTTLKNEKVIDFVDVSYKIRKFYEVGEASAKNVAQ

Sequences:

>Translated_474_residues
MTTILHEYYVGPAKIRILRDDNGICKYIVEEPQLTEYEEEIKNSILSEIMYTNLKNIEDFVISKLKDKGFKDDVIEKILY
HVKKSMLYDNITPLMLDQEIEEIECRGFGYPITVIHRSFSECIRLFTNIIPKNEDDIIKIVEKMANKANKSINIAKPYVE
FSLPEGHRVAATLGSEISLPGSTFDIRKFPSKPLSIIQMITNGMLNELIASYLWFIMEYKPFIMILGPTGSGKTSLLTAI
LNLINPSYKILTIEDTPEINITSDNWVRFISRSTLAGNYDVTLSDLAKLALRYRPDYLVVGEVRGKEIEALIHAAASGHG
SLTTFHGSRPIDAITRITDLLSSDLSKLFLQTIWSFIIVSRRKEGNKSIRSIIGIYETLIDKNKIKFKKVIEWSFIKNEF
IPLDINSLSKRSYRLKWISKVYGLSIEEIRQEIERRMNFLTTLKNEKVIDFVDVSYKIRKFYEVGEASAKNVAQ
>Mature_473_residues
TTILHEYYVGPAKIRILRDDNGICKYIVEEPQLTEYEEEIKNSILSEIMYTNLKNIEDFVISKLKDKGFKDDVIEKILYH
VKKSMLYDNITPLMLDQEIEEIECRGFGYPITVIHRSFSECIRLFTNIIPKNEDDIIKIVEKMANKANKSINIAKPYVEF
SLPEGHRVAATLGSEISLPGSTFDIRKFPSKPLSIIQMITNGMLNELIASYLWFIMEYKPFIMILGPTGSGKTSLLTAIL
NLINPSYKILTIEDTPEINITSDNWVRFISRSTLAGNYDVTLSDLAKLALRYRPDYLVVGEVRGKEIEALIHAAASGHGS
LTTFHGSRPIDAITRITDLLSSDLSKLFLQTIWSFIIVSRRKEGNKSIRSIIGIYETLIDKNKIKFKKVIEWSFIKNEFI
PLDINSLSKRSYRLKWISKVYGLSIEEIRQEIERRMNFLTTLKNEKVIDFVDVSYKIRKFYEVGEASAKNVAQ

Specific function: Involved In A General Secretion Pathway (Gsp) For The Export Of Proteins (By Similarity). [C]

COG id: COG0630

COG function: function code NU; Type IV secretory pathway, VirB11 components, and related ATPases involved in archaeal flagella biosynthesis

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP E family [H]

Homologues:

Organism=Escherichia coli, GI1789723, Length=140, Percent_Identity=33.5714285714286, Blast_Score=72, Evalue=5e-14,
Organism=Escherichia coli, GI1786296, Length=224, Percent_Identity=27.2321428571429, Blast_Score=71, Evalue=2e-13,
Organism=Escherichia coli, GI87082188, Length=222, Percent_Identity=26.5765765765766, Blast_Score=64, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001482 [H]

Pfam domain/function: PF00437 GSPII_E [H]

EC number: NA

Molecular weight: Translated: 54443; Mature: 54312

Theoretical pI: Translated: 8.60; Mature: 8.60

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTILHEYYVGPAKIRILRDDNGICKYIVEEPQLTEYEEEIKNSILSEIMYTNLKNIEDF
CCCEEHHHCCCCEEEEEEECCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
VISKLKDKGFKDDVIEKILYHVKKSMLYDNITPLMLDQEIEEIECRGFGYPITVIHRSFS
HHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHH
ECIRLFTNIIPKNEDDIIKIVEKMANKANKSINIAKPYVEFSLPEGHRVAATLGSEISLP
HHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCEEEECCEEEEECCCCCEEHEECCCCCCCC
GSTFDIRKFPSKPLSIIQMITNGMLNELIASYLWFIMEYKPFIMILGPTGSGKTSLLTAI
CCCCCHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHH
LNLINPSYKILTIEDTPEINITSDNWVRFISRSTLAGNYDVTLSDLAKLALRYRPDYLVV
HHHHCCCEEEEEEECCCCEEECCHHHHHHHHHHCCCCCCCEEHHHHHHHHHHCCCCEEEE
GEVRGKEIEALIHAAASGHGSLTTFHGSRPIDAITRITDLLSSDLSKLFLQTIWSFIIVS
ECCCCHHHHHHHHHHHCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RRKEGNKSIRSIIGIYETLIDKNKIKFKKVIEWSFIKNEFIPLDINSLSKRSYRLKWISK
HCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCEEECHHHHCCHHHHHHHHHH
VYGLSIEEIRQEIERRMNFLTTLKNEKVIDFVDVSYKIRKFYEVGEASAKNVAQ
HHCCCHHHHHHHHHHHHHHHHHHCCCCEEEEEHHHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure 
TTILHEYYVGPAKIRILRDDNGICKYIVEEPQLTEYEEEIKNSILSEIMYTNLKNIEDF
CCEEHHHCCCCEEEEEEECCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
VISKLKDKGFKDDVIEKILYHVKKSMLYDNITPLMLDQEIEEIECRGFGYPITVIHRSFS
HHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHH
ECIRLFTNIIPKNEDDIIKIVEKMANKANKSINIAKPYVEFSLPEGHRVAATLGSEISLP
HHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCEEEECCEEEEECCCCCEEHEECCCCCCCC
GSTFDIRKFPSKPLSIIQMITNGMLNELIASYLWFIMEYKPFIMILGPTGSGKTSLLTAI
CCCCCHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHH
LNLINPSYKILTIEDTPEINITSDNWVRFISRSTLAGNYDVTLSDLAKLALRYRPDYLVV
HHHHCCCEEEEEEECCCCEEECCHHHHHHHHHHCCCCCCCEEHHHHHHHHHHCCCCEEEE
GEVRGKEIEALIHAAASGHGSLTTFHGSRPIDAITRITDLLSSDLSKLFLQTIWSFIIVS
ECCCCHHHHHHHHHHHCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RRKEGNKSIRSIIGIYETLIDKNKIKFKKVIEWSFIKNEFIPLDINSLSKRSYRLKWISK
HCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCEEECHHHHCCHHHHHHHHHH
VYGLSIEEIRQEIERRMNFLTTLKNEKVIDFVDVSYKIRKFYEVGEASAKNVAQ
HHCCCHHHHHHHHHHHHHHHHHHCCCCEEEEEHHHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]