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The map label for this gene is eno

Identifier: 15921475

GI number: 15921475

Start: 1212877

End: 1214127

Strand: Direct

Name: eno

Synonym: ST1212

Alternate gene names: 15921475

Gene position: 1212877-1214127 (Clockwise)

Preceding gene: 15921471

Following gene: 15921476

Centisome position: 45.01

GC content: 33.09

Gene sequence:

>1251_bases
ATGAATGACTATTTCAGAATCAAAAAAATAAAGGGTTACCAGATTCTTGATTCTAGAGGAAATAAAACAATCAGAGTTAA
AATCGAGACATATGGAGGAATATCAGAAACTGGTGACGCACCAGCTGGAGCATCAAAGGGTAGTAGAGAGGCGATAGAAT
TAAGGGACAAAGATGGAGGAGTAACTAGGGCAGTCGAGTTAGTAAATACCTTAATTAACGATTCCTTAAGGGATTTTGAT
GTAAGGAATCAACTAGGAATTGACCAAACATTGATAAGAATGGATGGAACACCTAATAAGTCAAGAGTAGGCGGAAATAC
AACTATAGCTACATCAATAGCTGTAGCTAAAACAGCTGCAAAAGCTATGGGGTTAGAAATTTTCCAATACATAGGTGGGC
CAAGAGTAAGATATCTGCCAATACCATTATTAAATATTTTAAATGGAGGATTACACGCTGGAAATGAACTAAAAATTCAG
GAGTTTATTATAATACCTTTAAGTTTTGACAGCTTTCACGAAGCCTTATATGCTGCCGATGAAGTATACAAGCAGTTAAA
GGGAATAATAACAGAAAAATACGGAAAACTTTATACAATGCTAGGAGATGAAGGAGGAGTAGCGCCACCATTATCTAAAA
CAGAAGACGCATTAGATTTAGTATATACTGCTATAAAGAATTCTGGTTATGAGGATAAGATAGTAATGGGAATAGATGCA
GCTTCTTCTGATTTCTTTAACGGAAGTCAATATGAAATAGATGGGAAAAAATTAAGCCCAGATGAGATGATAGATTACTA
CATCCAATTAGCTTCACGTTATCCGTTATTATATATAGAAGATCCTTTTAACGAGAATGATTTTGAGAGATTTTCTATTT
TACAACAGAAGTTAAAGAAAACTATTGTTACTGGAGACGATTTATTTACCACTAACATTGAGTATCTAAAGAAAGGGATA
GAGAAAAGTTCTGCTAAAGGTACTATAGTTAAGCCTAATCAAATTGGTACTTTATCAGAGACATTTGAATACATAGAATT
TGCGAAGAAAAATTCTATAAAAATAATTGTAAGTCATAGAAGTGGAGAGACAGAAGATTCTTTTATAGCTGATTTAGCTG
TTGGAGTACAAAGTGATTTTATAAAGACTGGAGCACCAGCTAGAGGCGAAAGAACTAGTAAATATAATAGATTACTAGAA
ATAGAAAACGATTATGGAATAGAATATTACGGTAAAAAAATTTATCTTTAA

Upstream 100 bases:

>100_bases
TTTGCATAGATAGGAGTTAATATTCCATATATAACGAAGATCAGAAGACAACTACAGAATAACATATTAATCTTTGCTCT
TAATTGATAATCGAATTTAA

Downstream 100 bases:

>100_bases
GGTAGTAAAACTCCCACTATTATTAAAAACATTGCTAGAACTAGCATTAATACTACAAAAGTTCTAAAAGGCATATTAGG
TAGTTCTTTAAATGCTTTAA

Product: phosphopyruvate hydratase

Products: NA

Alternate protein names: 2-phospho-D-glycerate hydro-lyase; 2-phosphoglycerate dehydratase

Number of amino acids: Translated: 416; Mature: 416

Protein sequence:

>416_residues
MNDYFRIKKIKGYQILDSRGNKTIRVKIETYGGISETGDAPAGASKGSREAIELRDKDGGVTRAVELVNTLINDSLRDFD
VRNQLGIDQTLIRMDGTPNKSRVGGNTTIATSIAVAKTAAKAMGLEIFQYIGGPRVRYLPIPLLNILNGGLHAGNELKIQ
EFIIIPLSFDSFHEALYAADEVYKQLKGIITEKYGKLYTMLGDEGGVAPPLSKTEDALDLVYTAIKNSGYEDKIVMGIDA
ASSDFFNGSQYEIDGKKLSPDEMIDYYIQLASRYPLLYIEDPFNENDFERFSILQQKLKKTIVTGDDLFTTNIEYLKKGI
EKSSAKGTIVKPNQIGTLSETFEYIEFAKKNSIKIIVSHRSGETEDSFIADLAVGVQSDFIKTGAPARGERTSKYNRLLE
IENDYGIEYYGKKIYL

Sequences:

>Translated_416_residues
MNDYFRIKKIKGYQILDSRGNKTIRVKIETYGGISETGDAPAGASKGSREAIELRDKDGGVTRAVELVNTLINDSLRDFD
VRNQLGIDQTLIRMDGTPNKSRVGGNTTIATSIAVAKTAAKAMGLEIFQYIGGPRVRYLPIPLLNILNGGLHAGNELKIQ
EFIIIPLSFDSFHEALYAADEVYKQLKGIITEKYGKLYTMLGDEGGVAPPLSKTEDALDLVYTAIKNSGYEDKIVMGIDA
ASSDFFNGSQYEIDGKKLSPDEMIDYYIQLASRYPLLYIEDPFNENDFERFSILQQKLKKTIVTGDDLFTTNIEYLKKGI
EKSSAKGTIVKPNQIGTLSETFEYIEFAKKNSIKIIVSHRSGETEDSFIADLAVGVQSDFIKTGAPARGERTSKYNRLLE
IENDYGIEYYGKKIYL
>Mature_416_residues
MNDYFRIKKIKGYQILDSRGNKTIRVKIETYGGISETGDAPAGASKGSREAIELRDKDGGVTRAVELVNTLINDSLRDFD
VRNQLGIDQTLIRMDGTPNKSRVGGNTTIATSIAVAKTAAKAMGLEIFQYIGGPRVRYLPIPLLNILNGGLHAGNELKIQ
EFIIIPLSFDSFHEALYAADEVYKQLKGIITEKYGKLYTMLGDEGGVAPPLSKTEDALDLVYTAIKNSGYEDKIVMGIDA
ASSDFFNGSQYEIDGKKLSPDEMIDYYIQLASRYPLLYIEDPFNENDFERFSILQQKLKKTIVTGDDLFTTNIEYLKKGI
EKSSAKGTIVKPNQIGTLSETFEYIEFAKKNSIKIIVSHRSGETEDSFIADLAVGVQSDFIKTGAPARGERTSKYNRLLE
IENDYGIEYYGKKIYL

Specific function: Catalyzes the reversible conversion of 2- phosphoglycerate into phosphoenolpyruvate. It is essential for the degradation of carbohydrates via glycolysis

COG id: COG0148

COG function: function code G; Enolase

Gene ontology:

Cell location: Cytoplasm. Secreted. Cell surface. Note=Fractions of enolase are present in both the cytoplasm and on the cell surface. The export of enolase possibly depends on the covalent binding to the substrate; once secreted, it remains attached to the archaeal cel

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enolase family

Homologues:

Organism=Homo sapiens, GI4503571, Length=421, Percent_Identity=42.5178147268409, Blast_Score=330, Evalue=2e-90,
Organism=Homo sapiens, GI301897477, Length=421, Percent_Identity=41.5676959619952, Blast_Score=322, Evalue=6e-88,
Organism=Homo sapiens, GI301897469, Length=421, Percent_Identity=41.5676959619952, Blast_Score=322, Evalue=6e-88,
Organism=Homo sapiens, GI5803011, Length=420, Percent_Identity=40.9523809523809, Blast_Score=316, Evalue=3e-86,
Organism=Homo sapiens, GI301897479, Length=413, Percent_Identity=39.225181598063, Blast_Score=286, Evalue=3e-77,
Organism=Homo sapiens, GI169201331, Length=341, Percent_Identity=24.9266862170088, Blast_Score=83, Evalue=4e-16,
Organism=Homo sapiens, GI169201757, Length=341, Percent_Identity=24.9266862170088, Blast_Score=83, Evalue=4e-16,
Organism=Homo sapiens, GI239744207, Length=341, Percent_Identity=24.9266862170088, Blast_Score=83, Evalue=4e-16,
Organism=Escherichia coli, GI1789141, Length=426, Percent_Identity=41.7840375586854, Blast_Score=305, Evalue=4e-84,
Organism=Caenorhabditis elegans, GI17536383, Length=421, Percent_Identity=41.3301662707838, Blast_Score=315, Evalue=3e-86,
Organism=Caenorhabditis elegans, GI71995829, Length=421, Percent_Identity=41.3301662707838, Blast_Score=315, Evalue=3e-86,
Organism=Caenorhabditis elegans, GI32563855, Length=184, Percent_Identity=39.6739130434783, Blast_Score=145, Evalue=4e-35,
Organism=Saccharomyces cerevisiae, GI6323985, Length=436, Percent_Identity=41.0550458715596, Blast_Score=318, Evalue=8e-88,
Organism=Saccharomyces cerevisiae, GI6324974, Length=436, Percent_Identity=40.8256880733945, Blast_Score=317, Evalue=3e-87,
Organism=Saccharomyces cerevisiae, GI6324969, Length=436, Percent_Identity=40.8256880733945, Blast_Score=317, Evalue=3e-87,
Organism=Saccharomyces cerevisiae, GI6321693, Length=423, Percent_Identity=41.6075650118203, Blast_Score=306, Evalue=5e-84,
Organism=Saccharomyces cerevisiae, GI6321968, Length=423, Percent_Identity=40.4255319148936, Blast_Score=294, Evalue=2e-80,
Organism=Drosophila melanogaster, GI24580918, Length=427, Percent_Identity=41.92037470726, Blast_Score=305, Evalue=5e-83,
Organism=Drosophila melanogaster, GI24580916, Length=427, Percent_Identity=41.92037470726, Blast_Score=305, Evalue=5e-83,
Organism=Drosophila melanogaster, GI24580920, Length=427, Percent_Identity=41.92037470726, Blast_Score=305, Evalue=5e-83,
Organism=Drosophila melanogaster, GI24580914, Length=427, Percent_Identity=41.92037470726, Blast_Score=305, Evalue=5e-83,
Organism=Drosophila melanogaster, GI281360527, Length=427, Percent_Identity=41.92037470726, Blast_Score=304, Evalue=6e-83,
Organism=Drosophila melanogaster, GI17137654, Length=427, Percent_Identity=41.92037470726, Blast_Score=304, Evalue=6e-83,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1660 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 20 Molecules/Cell In: Stationary Phase,

Swissprot (AC and ID): ENO_SULTO (Q972B6)

Other databases:

- EMBL:   BA000023
- RefSeq:   NP_377144.1
- ProteinModelPortal:   Q972B6
- SMR:   Q972B6
- GeneID:   1459210
- GenomeReviews:   BA000023_GR
- KEGG:   sto:ST1212
- NMPDR:   fig|273063.1.peg.1312
- HOGENOM:   HBG726599
- OMA:   LICGDDL
- ProtClustDB:   PRK00077
- BioCyc:   STOK273063:ST1212-MONOMER
- BRENDA:   4.2.1.11
- GO:   GO:0006096
- HAMAP:   MF_00318
- InterPro:   IPR000941
- InterPro:   IPR020810
- InterPro:   IPR020809
- InterPro:   IPR020811
- PIRSF:   PIRSF001400
- PRINTS:   PR00148
- TIGRFAMs:   TIGR01060

Pfam domain/function: PF00113 Enolase_C; PF03952 Enolase_N

EC number: =4.2.1.11

Molecular weight: Translated: 46305; Mature: 46305

Theoretical pI: Translated: 5.26; Mature: 5.26

Prosite motif: PS00164 ENOLASE

Important sites: ACT_SITE 204-204 ACT_SITE 331-331 BINDING 152-152 BINDING 161-161 BINDING 280-280 BINDING 306-306 BINDING 331-331 BINDING 382-382

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
1.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNDYFRIKKIKGYQILDSRGNKTIRVKIETYGGISETGDAPAGASKGSREAIELRDKDGG
CCCCEEEEEECCEEEEECCCCEEEEEEEEECCCCCCCCCCCCCCCCCCCCEEEEECCCCC
VTRAVELVNTLINDSLRDFDVRNQLGIDQTLIRMDGTPNKSRVGGNTTIATSIAVAKTAA
HHHHHHHHHHHHCCCHHCCCCHHHCCCCCEEEEECCCCCCCCCCCCCEEHHHHHHHHHHH
KAMGLEIFQYIGGPRVRYLPIPLLNILNGGLHAGNELKIQEFIIIPLSFDSFHEALYAAD
HHHHHHHHHHHCCCEEEEECCCHHHHHCCCCCCCCCEEEEEEEEEEECHHHHHHHHHHHH
EVYKQLKGIITEKYGKLYTMLGDEGGVAPPLSKTEDALDLVYTAIKNSGYEDKIVMGIDA
HHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCEEEEEEEC
ASSDFFNGSQYEIDGKKLSPDEMIDYYIQLASRYPLLYIEDPFNENDFERFSILQQKLKK
CCCCCCCCCEEEECCCCCCHHHHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHHHHHHH
TIVTGDDLFTTNIEYLKKGIEKSSAKGTIVKPNQIGTLSETFEYIEFAKKNSIKIIVSHR
HHCCCCCEEECCHHHHHHHHHHCCCCCEEECCCCCCCHHHHHHHHHHHCCCCEEEEEEEC
SGETEDSFIADLAVGVQSDFIKTGAPARGERTSKYNRLLEIENDYGIEYYGKKIYL
CCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHEEEECCCCCEEEECCEECC
>Mature Secondary Structure
MNDYFRIKKIKGYQILDSRGNKTIRVKIETYGGISETGDAPAGASKGSREAIELRDKDGG
CCCCEEEEEECCEEEEECCCCEEEEEEEEECCCCCCCCCCCCCCCCCCCCEEEEECCCCC
VTRAVELVNTLINDSLRDFDVRNQLGIDQTLIRMDGTPNKSRVGGNTTIATSIAVAKTAA
HHHHHHHHHHHHCCCHHCCCCHHHCCCCCEEEEECCCCCCCCCCCCCEEHHHHHHHHHHH
KAMGLEIFQYIGGPRVRYLPIPLLNILNGGLHAGNELKIQEFIIIPLSFDSFHEALYAAD
HHHHHHHHHHHCCCEEEEECCCHHHHHCCCCCCCCCEEEEEEEEEEECHHHHHHHHHHHH
EVYKQLKGIITEKYGKLYTMLGDEGGVAPPLSKTEDALDLVYTAIKNSGYEDKIVMGIDA
HHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCEEEEEEEC
ASSDFFNGSQYEIDGKKLSPDEMIDYYIQLASRYPLLYIEDPFNENDFERFSILQQKLKK
CCCCCCCCCEEEECCCCCCHHHHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHHHHHHH
TIVTGDDLFTTNIEYLKKGIEKSSAKGTIVKPNQIGTLSETFEYIEFAKKNSIKIIVSHR
HHCCCCCEEECCHHHHHHHHHHCCCCCEEECCCCCCCHHHHHHHHHHHCCCCEEEEEEEC
SGETEDSFIADLAVGVQSDFIKTGAPARGERTSKYNRLLEIENDYGIEYYGKKIYL
CCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHEEEECCCCCEEEECCEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11572479