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The map label for this gene is nadB

Identifier: 15921459

GI number: 15921459

Start: 1198164

End: 1199582

Strand: Direct

Name: nadB

Synonym: ST1196

Alternate gene names: 15921459

Gene position: 1198164-1199582 (Clockwise)

Preceding gene: 15921458

Following gene: 15921470

Centisome position: 44.46

GC content: 35.73

Gene sequence:

>1419_bases
ATGATCTATATAATTGGTTCAGGTATTGCTGGTTTATCTGCTGGTGTTGCGTTAAGAAGGGCTGGAAAGAAAGTTACTTT
AATAAGTAAAAGAATTGATGGTGGTTCTACTCCCATAGCTAAGGGTGGTGTTGCAGCTTCTGTTGGAAGCGATGATAGTC
CAGAGCTACATGCTCAAGACACTATTAGGGTTGGTGATGGATTGTGCGATGTAAAGACTGTTAATTATGTTACATCTGAG
GCTAAGAATGTTATAGAGACTTTTGAGAGTTGGGGTTTTGAGTTTGAGGAGGATTTAAGGTTAGAAGGAGGTCATACAAA
GAGGAGAGTTTTGCATAGGACTGATGAGACGGGAAGAGAGATATTCAACTTTTTATTAAAGCTTGCTAGGGAGGAAGGAA
TTCCCATAATTGAGGATAGACTTGTTGAGATAAGGGTTAAAGACGGGAAGGTTACTGGTTTTGTTACGGAAAAGAGGGGT
TTGGTTGAAGATGTTGATAAACTTGTTTTAGCTACTGGTGGTTATTCTTATCTTTATGAATACTCTTCTACTCAATCTAC
TAATATTGGGGATGGTATGGCTATTGCTTTTAAGGCTGGGACTATACTTGCTGATATGGAGTTTGTACAATTTCATCCTA
CTGTTACAAGTTTAGACGGAGAAGTTTTCTTACTAACAGAAACTTTAAGGGGTGAGGGAGCACAAATCATTAATGAGAAT
GGAGAAAGGTTTTTATTTAATTATGATAAGAGAGGAGAATTAGCACCTAGGGATATTCTTTCTAGGGCTATTTATATTGA
AATGTTAAAGGGACATAAGGTTTTTATTGATTTGAGTAAAATTGAGGATTTTGAAAGAAAGTTCCCTGTTGTTGCTAAGT
ATTTAGCTAGGCATGGTCATAATTACAAAGTTAAGATTCCTATATTTCCTGCAGCTCATTTTGTTGATGGAGGTATTAGG
GTTAATATTAGAGGGGAAAGTAATATTGTAAATCTTTATGCTATTGGCGAGGTTAGTGATTCTGGTTTACATGGTGCTAA
TAGGTTAGCTAGTAATTCTCTTTTAGAAGGTTTGGTTTTTGGCATTAATTTGCCAAGATATGTTGATAGTAGTTGGGAGG
GAATATCTACTGATGACGGTATTGTTCATTCTGTTAGGATATCTGGTAATAAGACTCTGTCTCTTAAAGAGATAAGGAGG
ATCAATTGGGAAAATGTTGGTATAATAAGGAATGAGGAGAAATTGGTTAAGGCTATAAATACTTATTCTAGTTCTACGCA
AAATGAGGCTATTATATCTTATTTGACCGCATTAGCTGCTGAAATAAGAAAGGAGAGTAGGGGTAATCATTTTAGGGAGG
ATTATCCTTATAAGGATCCTAATTGGGAAAAAAGGATTTATTTTAAGTTAGTTGTTTAG

Upstream 100 bases:

>100_bases
TTATTTAGATATGTATGATGCTTGCTGTAGTTTACCTTTAGAGTATTTAAATAAATTTAAACTTTAATTTTTTAAACTCC
GAACACATGTTCGATTATAT

Downstream 100 bases:

>100_bases
TAGTCTCATTGTCCTTTCAAATGCTTCTTTTGCCCTTTCTGCTATATCCTTAGGTACTTTCACTTCATAAATTTCTTCTT
CTAGTGATCTTTTTATTTTT

Product: L-aspartate oxidase

Products: NA

Alternate protein names: LASPO; Quinolinate synthase B

Number of amino acids: Translated: 472; Mature: 472

Protein sequence:

>472_residues
MIYIIGSGIAGLSAGVALRRAGKKVTLISKRIDGGSTPIAKGGVAASVGSDDSPELHAQDTIRVGDGLCDVKTVNYVTSE
AKNVIETFESWGFEFEEDLRLEGGHTKRRVLHRTDETGREIFNFLLKLAREEGIPIIEDRLVEIRVKDGKVTGFVTEKRG
LVEDVDKLVLATGGYSYLYEYSSTQSTNIGDGMAIAFKAGTILADMEFVQFHPTVTSLDGEVFLLTETLRGEGAQIINEN
GERFLFNYDKRGELAPRDILSRAIYIEMLKGHKVFIDLSKIEDFERKFPVVAKYLARHGHNYKVKIPIFPAAHFVDGGIR
VNIRGESNIVNLYAIGEVSDSGLHGANRLASNSLLEGLVFGINLPRYVDSSWEGISTDDGIVHSVRISGNKTLSLKEIRR
INWENVGIIRNEEKLVKAINTYSSSTQNEAIISYLTALAAEIRKESRGNHFREDYPYKDPNWEKRIYFKLVV

Sequences:

>Translated_472_residues
MIYIIGSGIAGLSAGVALRRAGKKVTLISKRIDGGSTPIAKGGVAASVGSDDSPELHAQDTIRVGDGLCDVKTVNYVTSE
AKNVIETFESWGFEFEEDLRLEGGHTKRRVLHRTDETGREIFNFLLKLAREEGIPIIEDRLVEIRVKDGKVTGFVTEKRG
LVEDVDKLVLATGGYSYLYEYSSTQSTNIGDGMAIAFKAGTILADMEFVQFHPTVTSLDGEVFLLTETLRGEGAQIINEN
GERFLFNYDKRGELAPRDILSRAIYIEMLKGHKVFIDLSKIEDFERKFPVVAKYLARHGHNYKVKIPIFPAAHFVDGGIR
VNIRGESNIVNLYAIGEVSDSGLHGANRLASNSLLEGLVFGINLPRYVDSSWEGISTDDGIVHSVRISGNKTLSLKEIRR
INWENVGIIRNEEKLVKAINTYSSSTQNEAIISYLTALAAEIRKESRGNHFREDYPYKDPNWEKRIYFKLVV
>Mature_472_residues
MIYIIGSGIAGLSAGVALRRAGKKVTLISKRIDGGSTPIAKGGVAASVGSDDSPELHAQDTIRVGDGLCDVKTVNYVTSE
AKNVIETFESWGFEFEEDLRLEGGHTKRRVLHRTDETGREIFNFLLKLAREEGIPIIEDRLVEIRVKDGKVTGFVTEKRG
LVEDVDKLVLATGGYSYLYEYSSTQSTNIGDGMAIAFKAGTILADMEFVQFHPTVTSLDGEVFLLTETLRGEGAQIINEN
GERFLFNYDKRGELAPRDILSRAIYIEMLKGHKVFIDLSKIEDFERKFPVVAKYLARHGHNYKVKIPIFPAAHFVDGGIR
VNIRGESNIVNLYAIGEVSDSGLHGANRLASNSLLEGLVFGINLPRYVDSSWEGISTDDGIVHSVRISGNKTLSLKEIRR
INWENVGIIRNEEKLVKAINTYSSSTQNEAIISYLTALAAEIRKESRGNHFREDYPYKDPNWEKRIYFKLVV

Specific function: Catalyzes the oxidation of L-aspartate to iminoaspartate

COG id: COG0029

COG function: function code H; Aspartate oxidase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FAD-dependent oxidoreductase 2 family. NadB subfamily

Homologues:

Organism=Homo sapiens, GI156416003, Length=523, Percent_Identity=27.7246653919694, Blast_Score=163, Evalue=3e-40,
Organism=Escherichia coli, GI1788928, Length=520, Percent_Identity=37.3076923076923, Blast_Score=269, Evalue=2e-73,
Organism=Escherichia coli, GI1790597, Length=377, Percent_Identity=35.0132625994695, Blast_Score=184, Evalue=1e-47,
Organism=Escherichia coli, GI1786942, Length=532, Percent_Identity=28.5714285714286, Blast_Score=157, Evalue=1e-39,
Organism=Caenorhabditis elegans, GI17550100, Length=540, Percent_Identity=27.037037037037, Blast_Score=160, Evalue=2e-39,
Organism=Caenorhabditis elegans, GI17505833, Length=534, Percent_Identity=28.2771535580524, Blast_Score=158, Evalue=5e-39,
Organism=Caenorhabditis elegans, GI71986328, Length=459, Percent_Identity=28.1045751633987, Blast_Score=99, Evalue=6e-21,
Organism=Saccharomyces cerevisiae, GI6322701, Length=532, Percent_Identity=29.8872180451128, Blast_Score=174, Evalue=3e-44,
Organism=Saccharomyces cerevisiae, GI6322416, Length=537, Percent_Identity=29.0502793296089, Blast_Score=165, Evalue=2e-41,
Organism=Saccharomyces cerevisiae, GI6320788, Length=466, Percent_Identity=28.3261802575107, Blast_Score=105, Evalue=2e-23,
Organism=Saccharomyces cerevisiae, GI6322511, Length=461, Percent_Identity=27.3318872017354, Blast_Score=85, Evalue=2e-17,
Organism=Drosophila melanogaster, GI17137288, Length=529, Percent_Identity=26.0869565217391, Blast_Score=156, Evalue=3e-38,
Organism=Drosophila melanogaster, GI24655642, Length=529, Percent_Identity=26.0869565217391, Blast_Score=156, Evalue=3e-38,
Organism=Drosophila melanogaster, GI24655647, Length=529, Percent_Identity=26.0869565217391, Blast_Score=156, Evalue=3e-38,
Organism=Drosophila melanogaster, GI24663005, Length=528, Percent_Identity=28.4090909090909, Blast_Score=140, Evalue=2e-33,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NADB_SULTO (Q972D2)

Other databases:

- EMBL:   BA000023
- RefSeq:   NP_377128.1
- PDB:   2E5V
- PDBsum:   2E5V
- ProteinModelPortal:   Q972D2
- SMR:   Q972D2
- GeneID:   1459193
- GenomeReviews:   BA000023_GR
- KEGG:   sto:ST1196
- NMPDR:   fig|273063.1.peg.1296
- HOGENOM:   HBG293998
- OMA:   RRINWEN
- ProtClustDB:   CLSK883692
- BioCyc:   STOK273063:ST1196-MONOMER
- BRENDA:   1.4.3.16
- GO:   GO:0005737
- InterPro:   IPR003953
- InterPro:   IPR013027
- InterPro:   IPR015939
- InterPro:   IPR004112
- Gene3D:   G3DSA:1.20.58.100
- PRINTS:   PR00368

Pfam domain/function: PF00890 FAD_binding_2; PF02910 Succ_DH_flav_C; SSF46977 Succ_DH_flav_C

EC number: =1.4.3.16

Molecular weight: Translated: 52582; Mature: 52582

Theoretical pI: Translated: 6.60; Mature: 6.60

Prosite motif: NA

Important sites: ACT_SITE 212-212 ACT_SITE 230-230

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
1.1 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
1.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIYIIGSGIAGLSAGVALRRAGKKVTLISKRIDGGSTPIAKGGVAASVGSDDSPELHAQD
CEEEEECCCCHHHHHHHHHHCCCEEEEEEEECCCCCCCCCCCCEEEECCCCCCCCCCCCC
TIRVGDGLCDVKTVNYVTSEAKNVIETFESWGFEFEEDLRLEGGHTKRRVLHRTDETGRE
EEEECCCCCCEEHHHHHHHHHHHHHHHHHHCCCCCCCCCEECCCCHHHHHHHCCCHHHHH
IFNFLLKLAREEGIPIIEDRLVEIRVKDGKVTGFVTEKRGLVEDVDKLVLATGGYSYLYE
HHHHHHHHHHHCCCCEECCCEEEEEEECCEEEEEEECCCCHHHHHHHHEEECCCCEEEEE
YSSTQSTNIGDGMAIAFKAGTILADMEFVQFHPTVTSLDGEVFLLTETLRGEGAQIINEN
ECCCCCCCCCCCEEEEEECCEEEEEEEEEEECCCEEECCCCEEEEEEECCCCCCEEECCC
GERFLFNYDKRGELAPRDILSRAIYIEMLKGHKVFIDLSKIEDFERKFPVVAKYLARHGH
CCEEEEEECCCCCCCHHHHHHHHHHHHEECCCEEEEEHHHHHHHHHHHHHHHHHHHHCCC
NYKVKIPIFPAAHFVDGGIRVNIRGESNIVNLYAIGEVSDSGLHGANRLASNSLLEGLVF
CEEEEEEEECCHHEECCCEEEEEECCCCEEEEEEEECCCCCCCCCHHHHHHHHHHHHHHH
GINLPRYVDSSWEGISTDDGIVHSVRISGNKTLSLKEIRRINWENVGIIRNEEKLVKAIN
CCCCCHHHCCCCCCCCCCCCEEEEEEECCCCEEEHHHHHHCCCCCCEEEECHHHHHHHHH
TYSSSTQNEAIISYLTALAAEIRKESRGNHFREDYPYKDPNWEKRIYFKLVV
HHCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEEEEEC
>Mature Secondary Structure
MIYIIGSGIAGLSAGVALRRAGKKVTLISKRIDGGSTPIAKGGVAASVGSDDSPELHAQD
CEEEEECCCCHHHHHHHHHHCCCEEEEEEEECCCCCCCCCCCCEEEECCCCCCCCCCCCC
TIRVGDGLCDVKTVNYVTSEAKNVIETFESWGFEFEEDLRLEGGHTKRRVLHRTDETGRE
EEEECCCCCCEEHHHHHHHHHHHHHHHHHHCCCCCCCCCEECCCCHHHHHHHCCCHHHHH
IFNFLLKLAREEGIPIIEDRLVEIRVKDGKVTGFVTEKRGLVEDVDKLVLATGGYSYLYE
HHHHHHHHHHHCCCCEECCCEEEEEEECCEEEEEEECCCCHHHHHHHHEEECCCCEEEEE
YSSTQSTNIGDGMAIAFKAGTILADMEFVQFHPTVTSLDGEVFLLTETLRGEGAQIINEN
ECCCCCCCCCCCEEEEEECCEEEEEEEEEEECCCEEECCCCEEEEEEECCCCCCEEECCC
GERFLFNYDKRGELAPRDILSRAIYIEMLKGHKVFIDLSKIEDFERKFPVVAKYLARHGH
CCEEEEEECCCCCCCHHHHHHHHHHHHEECCCEEEEEHHHHHHHHHHHHHHHHHHHHCCC
NYKVKIPIFPAAHFVDGGIRVNIRGESNIVNLYAIGEVSDSGLHGANRLASNSLLEGLVF
CEEEEEEEECCHHEECCCEEEEEECCCCEEEEEEEECCCCCCCCCHHHHHHHHHHHHHHH
GINLPRYVDSSWEGISTDDGIVHSVRISGNKTLSLKEIRRINWENVGIIRNEEKLVKAIN
CCCCCHHHCCCCCCCCCCCCEEEEEEECCCCEEEHHHHHHCCCCCCEEEECHHHHHHHHH
TYSSSTQNEAIISYLTALAAEIRKESRGNHFREDYPYKDPNWEKRIYFKLVV
HHCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11572479