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The map label for this gene is bioB [C]

Identifier: 15921457

GI number: 15921457

Start: 1196485

End: 1197312

Strand: Direct

Name: bioB [C]

Synonym: ST1194

Alternate gene names: 15921457

Gene position: 1196485-1197312 (Clockwise)

Preceding gene: 15921456

Following gene: 15921458

Centisome position: 44.4

GC content: 33.94

Gene sequence:

>828_bases
GTGTGTCAAAAAATGAAAGTTCTACTTTCTTCGGGTACTTACTACTATTTAAAAAGGGGTATAAAGGTTAGTGAGACTGC
TTATGCTTTACAATATGGTGGTTGTGAAGGTAAGTGTAAGTTCTGTACGCAATCTGTCTTATCTAATGCTGATAAGACTT
ATTTATCTAGGGTTAAGTGGTATTTAGTTGATTTTAATGATGAGACTGCTGAAAAACTTTCCATTTTTTCCCGTTTTTGT
TTACAAACTGTAATAAAGAAGGGTTTTGTTGATGAGGTTATTGATATTCTTAAGCTTACTAGAAATAAGCATAAATCTGT
TACCATAACCCCCATAGAGGTAAGCTATTTGTATAAATTTAAGGAGTTAAATGTTGACTATTTAGGCGTTGGTTTGGATA
CTGTTAAGAGGCTTTGGGATTATGTTGGTAAGCCTTATACTTTTGATGAGTATATGAAATTTATAAAGGAAGCTGTTAGA
GTCTTTGGAAGGGGTCATGTTTACGCTCATTTAATAGTTGGTCTTGGTGAGACTGATGATGAATTAATTAGTTTAATGAA
AGAGTTAAAAGATATTGGTGCTGAGGTTGCTTTGTTTGCTTTTACCCCGGTTAAGGGTACTCCTTTTGAGAATTTAGAGA
GACCTTCTCTTGAAAGGTATAGGCACATTCAAAGAATGAGATACATAATATTTGAGGGAAAGAATTCTAAGCTTGCATAC
CTAACATCTGGTTGTCCATCTTGTGACAGACCTTATTATAATGAAGATCCTAGAGATAAGTTGTATAATATTCCTTTGAG
GGATTTCCGTTGGTATATGGGTTCGTAA

Upstream 100 bases:

>100_bases
ATGATTTGAGATTGATAGAAGATAGTTTAAGTAAAGGTAAGAGACCCAATGCTTTTATTTTAGGTGTTGATATTGATGAG
TTATATGAGAAGTTCTTAGA

Downstream 100 bases:

>100_bases
TTTTGAGAAAATTTTCTGTTATAAGCATATCTGGTGGTTCTTGTTCTTTAAGTTGCACATATTGTAATTCAACATATATA
AAGAGTATGGAGCCTGCTAT

Product: hypothetical protein

Products: Biotin [C]

Alternate protein names: Radical SAM Domain-Containing Protein; Biotin Synthase Related Protein; Biotin Synthase; Biotin Synthase-Related Protein; Radical SAM Superfamily Protein; Iron-Molybdenum Cofactorbiosynthesis Nitrogenase NifB; Biotin Synthase-Related; Radical SAM Family Protein

Number of amino acids: Translated: 275; Mature: 275

Protein sequence:

>275_residues
MCQKMKVLLSSGTYYYLKRGIKVSETAYALQYGGCEGKCKFCTQSVLSNADKTYLSRVKWYLVDFNDETAEKLSIFSRFC
LQTVIKKGFVDEVIDILKLTRNKHKSVTITPIEVSYLYKFKELNVDYLGVGLDTVKRLWDYVGKPYTFDEYMKFIKEAVR
VFGRGHVYAHLIVGLGETDDELISLMKELKDIGAEVALFAFTPVKGTPFENLERPSLERYRHIQRMRYIIFEGKNSKLAY
LTSGCPSCDRPYYNEDPRDKLYNIPLRDFRWYMGS

Sequences:

>Translated_275_residues
MCQKMKVLLSSGTYYYLKRGIKVSETAYALQYGGCEGKCKFCTQSVLSNADKTYLSRVKWYLVDFNDETAEKLSIFSRFC
LQTVIKKGFVDEVIDILKLTRNKHKSVTITPIEVSYLYKFKELNVDYLGVGLDTVKRLWDYVGKPYTFDEYMKFIKEAVR
VFGRGHVYAHLIVGLGETDDELISLMKELKDIGAEVALFAFTPVKGTPFENLERPSLERYRHIQRMRYIIFEGKNSKLAY
LTSGCPSCDRPYYNEDPRDKLYNIPLRDFRWYMGS
>Mature_275_residues
MCQKMKVLLSSGTYYYLKRGIKVSETAYALQYGGCEGKCKFCTQSVLSNADKTYLSRVKWYLVDFNDETAEKLSIFSRFC
LQTVIKKGFVDEVIDILKLTRNKHKSVTITPIEVSYLYKFKELNVDYLGVGLDTVKRLWDYVGKPYTFDEYMKFIKEAVR
VFGRGHVYAHLIVGLGETDDELISLMKELKDIGAEVALFAFTPVKGTPFENLERPSLERYRHIQRMRYIIFEGKNSKLAY
LTSGCPSCDRPYYNEDPRDKLYNIPLRDFRWYMGS

Specific function: Biotin biosynthesis; last step. [C]

COG id: COG2516

COG function: function code R; Biotin synthase-related enzyme

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: 2.8.1.6 [C]

Molecular weight: Translated: 32042; Mature: 32042

Theoretical pI: Translated: 9.00; Mature: 9.00

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MCQKMKVLLSSGTYYYLKRGIKVSETAYALQYGGCEGKCKFCTQSVLSNADKTYLSRVKW
CCCHHHHHHHCCCEEEHHCCCEECHHHEEEEECCCCCHHHHHHHHHHCCCHHHHHHHHEE
YLVDFNDETAEKLSIFSRFCLQTVIKKGFVDEVIDILKLTRNKHKSVTITPIEVSYLYKF
EEEECCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCEEEEEEEEHHHHHHH
KELNVDYLGVGLDTVKRLWDYVGKPYTFDEYMKFIKEAVRVFGRGHVYAHLIVGLGETDD
HHCCCEEEECCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCEEEEEEEECCCCHH
ELISLMKELKDIGAEVALFAFTPVKGTPFENLERPSLERYRHIQRMRYIIFEGKNSKLAY
HHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHEEEEECCCCEEEE
LTSGCPSCDRPYYNEDPRDKLYNIPLRDFRWYMGS
EECCCCCCCCCCCCCCCHHHEECCCHHHHHHHCCC
>Mature Secondary Structure
MCQKMKVLLSSGTYYYLKRGIKVSETAYALQYGGCEGKCKFCTQSVLSNADKTYLSRVKW
CCCHHHHHHHCCCEEEHHCCCEECHHHEEEEECCCCCHHHHHHHHHHCCCHHHHHHHHEE
YLVDFNDETAEKLSIFSRFCLQTVIKKGFVDEVIDILKLTRNKHKSVTITPIEVSYLYKF
EEEECCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCEEEEEEEEHHHHHHH
KELNVDYLGVGLDTVKRLWDYVGKPYTFDEYMKFIKEAVRVFGRGHVYAHLIVGLGETDD
HHCCCEEEECCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCEEEEEEEECCCCHH
ELISLMKELKDIGAEVALFAFTPVKGTPFENLERPSLERYRHIQRMRYIIFEGKNSKLAY
HHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHEEEEECCCCEEEE
LTSGCPSCDRPYYNEDPRDKLYNIPLRDFRWYMGS
EECCCCCCCCCCCCCCCHHHEECCCHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Fe2+ [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.002 {dethiobiotin}} [C]

Substrates: Dethiobiotin; sulfur [C]

Specific reaction: Dethiobiotin + sulfur = biotin [C]

General reaction: Sulfur atom transfer [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA