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The map label for this gene is yafV [C]

Identifier: 15921376

GI number: 15921376

Start: 1113079

End: 1113924

Strand: Direct

Name: yafV [C]

Synonym: ST1121

Alternate gene names: 15921376

Gene position: 1113079-1113924 (Clockwise)

Preceding gene: 15921375

Following gene: 15921378

Centisome position: 41.31

GC content: 33.33

Gene sequence:

>846_bases
ATGAAACACATGGTTAAAATTGCAATGATACAAATGGGAAGTGTAGAATCTAAGGAAGCAAATATACAGAAAGCTCTAGA
GTATACAAAGGCCGCAGTTAAAGATGGAGCAGAACTAATAGTATATAACGAGTTATTTACAACACAATATTTCCCCGCTA
CAGAAGATCCAAAATTCTTCGATTTAGCAGAGCCAGAAGACGGGCCTACGGTAAGAGTTTTTGCTGAATTTTCTAAACAG
TACAAAATAGGTATGATAATAACTATCTTTGAAGAAGATAAAAAAATTAAAGGAATATATTATGATACAGCTATTTTCAT
AAAAGATGGCAAGGTATTGGGTAAATATAGGAAAACTCATATACCTCAAGTGCCTGGTTACTATGAGAAGTTCTATTTCA
AACCAGGGAAAGAATATCCAGTATTCGATTTCGGAGGATATAAGATTGGGGCAGTAATATGTTATGATCGTCATTTTCCT
GAAGGTGTTAGAATACTAACACTGAAGGGTGCAGATATAGTTACAATACCTACTACAACAAACTTTTATCCCGAAACCTG
GGAACTAGAATTAAGAGCTCATGCTGCATTTAACACAATATACGTCGTGGGAGTAAATAGGACTCCAGAAATTTTCCAAG
GTAAGGAAATAGATTATTTTGGCAAAAGTTTAGTAGCAGATCCTACAGGAAATATATTAAAAGAAATGAGTAGCCAAGAA
GGTTATGAAATAGTCGATGTTAATCTTGATTTTATTAGAGAAAGAAGGAAAAAAGCTCCATTCTTAAGAGATAGAAAGCC
AGAAAACTATACAGAAATTTCATCATTATATATTGAAACCATATGA

Upstream 100 bases:

>100_bases
ATGAGAGGGTTGGCTGCGAAGAAGATGACAACGTACGAAGTATTGGAGAAAGTAACAAAAAGGAGAACTAGTGTTGATGA
AGAAATTAATACAGTAAGAG

Downstream 100 bases:

>100_bases
TTTTTTATTTCATGATTTCGTTAATCATATGTTAATGATTTATTTAAGATATACTACGAAGAATTAATGTATTAAAGAGA
AAGTTAAAATATTTTTTAAT

Product: beta-ureidopropionase

Products: NA

Alternate protein names: D-N-alpha-carbamilase [H]

Number of amino acids: Translated: 281; Mature: 281

Protein sequence:

>281_residues
MKHMVKIAMIQMGSVESKEANIQKALEYTKAAVKDGAELIVYNELFTTQYFPATEDPKFFDLAEPEDGPTVRVFAEFSKQ
YKIGMIITIFEEDKKIKGIYYDTAIFIKDGKVLGKYRKTHIPQVPGYYEKFYFKPGKEYPVFDFGGYKIGAVICYDRHFP
EGVRILTLKGADIVTIPTTTNFYPETWELELRAHAAFNTIYVVGVNRTPEIFQGKEIDYFGKSLVADPTGNILKEMSSQE
GYEIVDVNLDFIRERRKKAPFLRDRKPENYTEISSLYIETI

Sequences:

>Translated_281_residues
MKHMVKIAMIQMGSVESKEANIQKALEYTKAAVKDGAELIVYNELFTTQYFPATEDPKFFDLAEPEDGPTVRVFAEFSKQ
YKIGMIITIFEEDKKIKGIYYDTAIFIKDGKVLGKYRKTHIPQVPGYYEKFYFKPGKEYPVFDFGGYKIGAVICYDRHFP
EGVRILTLKGADIVTIPTTTNFYPETWELELRAHAAFNTIYVVGVNRTPEIFQGKEIDYFGKSLVADPTGNILKEMSSQE
GYEIVDVNLDFIRERRKKAPFLRDRKPENYTEISSLYIETI
>Mature_281_residues
MKHMVKIAMIQMGSVESKEANIQKALEYTKAAVKDGAELIVYNELFTTQYFPATEDPKFFDLAEPEDGPTVRVFAEFSKQ
YKIGMIITIFEEDKKIKGIYYDTAIFIKDGKVLGKYRKTHIPQVPGYYEKFYFKPGKEYPVFDFGGYKIGAVICYDRHFP
EGVRILTLKGADIVTIPTTTNFYPETWELELRAHAAFNTIYVVGVNRTPEIFQGKEIDYFGKSLVADPTGNILKEMSSQE
GYEIVDVNLDFIRERRKKAPFLRDRKPENYTEISSLYIETI

Specific function: The enzyme catalyzes the hydrolysis of N-carbamoyl-D- amino acids to the corresponding which are useful intermediates in the preparation of beta-lactam antibiotics. Industrial production of beta-lactam antibiotics is now being developed using this enzyme

COG id: COG0388

COG function: function code R; Predicted amidohydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 CN hydrolase domain [H]

Homologues:

Organism=Homo sapiens, GI297632350, Length=280, Percent_Identity=27.8571428571429, Blast_Score=112, Evalue=3e-25,
Organism=Homo sapiens, GI297632348, Length=280, Percent_Identity=27.8571428571429, Blast_Score=112, Evalue=3e-25,
Organism=Homo sapiens, GI5031947, Length=280, Percent_Identity=27.8571428571429, Blast_Score=112, Evalue=4e-25,
Organism=Homo sapiens, GI9910460, Length=275, Percent_Identity=28.7272727272727, Blast_Score=111, Evalue=9e-25,
Organism=Homo sapiens, GI7706509, Length=243, Percent_Identity=29.2181069958848, Blast_Score=101, Evalue=7e-22,
Organism=Caenorhabditis elegans, GI17533173, Length=304, Percent_Identity=28.6184210526316, Blast_Score=111, Evalue=5e-25,
Organism=Caenorhabditis elegans, GI17556280, Length=275, Percent_Identity=26.5454545454545, Blast_Score=87, Evalue=1e-17,
Organism=Saccharomyces cerevisiae, GI6323383, Length=289, Percent_Identity=30.1038062283737, Blast_Score=114, Evalue=2e-26,
Organism=Drosophila melanogaster, GI21358471, Length=281, Percent_Identity=29.1814946619217, Blast_Score=117, Evalue=6e-27,
Organism=Drosophila melanogaster, GI21355835, Length=280, Percent_Identity=26.7857142857143, Blast_Score=97, Evalue=1e-20,
Organism=Drosophila melanogaster, GI17933642, Length=273, Percent_Identity=26.3736263736264, Blast_Score=78, Evalue=5e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003010 [H]

Pfam domain/function: PF00795 CN_hydrolase [H]

EC number: =3.5.1.77 [H]

Molecular weight: Translated: 32403; Mature: 32403

Theoretical pI: Translated: 6.17; Mature: 6.17

Prosite motif: PS50263 CN_HYDROLASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKHMVKIAMIQMGSVESKEANIQKALEYTKAAVKDGAELIVYNELFTTQYFPATEDPKFF
CCCEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHCCCEEEEEEEHEEEECCCCCCCCCEE
DLAEPEDGPTVRVFAEFSKQYKIGMIITIFEEDKKIKGIYYDTAIFIKDGKVLGKYRKTH
ECCCCCCCCEEEEEEECCCCEEEEEEEEEEECCCEEEEEEEEEEEEEECCCEEHHHHHCC
IPQVPGYYEKFYFKPGKEYPVFDFGGYKIGAVICYDRHFPEGVRILTLKGADIVTIPTTT
CCCCCCHHHHEEECCCCCCCEEECCCEEEEEEEEECCCCCCCEEEEEECCCCEEEECCCC
NFYPETWELELRAHAAFNTIYVVGVNRTPEIFQGKEIDYFGKSLVADPTGNILKEMSSQE
CCCCCEEEEEEEEEEEEEEEEEEEECCCCHHHCCCCCCHHCCEEECCCCHHHHHHHHCCC
GYEIVDVNLDFIRERRKKAPFLRDRKPENYTEISSLYIETI
CCEEEECCHHHHHHHHHCCCCCCCCCCCCHHEEEEEEEEEC
>Mature Secondary Structure
MKHMVKIAMIQMGSVESKEANIQKALEYTKAAVKDGAELIVYNELFTTQYFPATEDPKFF
CCCEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHCCCEEEEEEEHEEEECCCCCCCCCEE
DLAEPEDGPTVRVFAEFSKQYKIGMIITIFEEDKKIKGIYYDTAIFIKDGKVLGKYRKTH
ECCCCCCCCEEEEEEECCCCEEEEEEEEEEECCCEEEEEEEEEEEEEECCCEEHHHHHCC
IPQVPGYYEKFYFKPGKEYPVFDFGGYKIGAVICYDRHFPEGVRILTLKGADIVTIPTTT
CCCCCCHHHHEEECCCCCCCEEECCCEEEEEEEEECCCCCCCEEEEEECCCCEEEECCCC
NFYPETWELELRAHAAFNTIYVVGVNRTPEIFQGKEIDYFGKSLVADPTGNILKEMSSQE
CCCCCEEEEEEEEEEEEEEEEEEEECCCCHHHCCCCCCHHCCEEECCCCHHHHHHHHCCC
GYEIVDVNLDFIRERRKKAPFLRDRKPENYTEISSLYIETI
CCEEEECCHHHHHHHHHCCCCCCCCCCCCHHEEEEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9648217; 10903946 [H]