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The map label for this gene is maeB [C]

Identifier: 15920468

GI number: 15920468

Start: 295592

End: 296923

Strand: Reverse

Name: maeB [C]

Synonym: ST0278

Alternate gene names: 15920468

Gene position: 296923-295592 (Counterclockwise)

Preceding gene: 15920470

Following gene: 15920467

Centisome position: 11.02

GC content: 34.23

Gene sequence:

>1332_bases
ATGCTCAAAAAATTTAATCTTATATTTAGTAAAGATACTGGTGTTATTACATTGAGTGAATTAGATAAAAAGGCAATTGA
AATTTCTTCTAAATATAAGGGAAAAATACAAATATTACCTAAGGTTCCTATAAAATCCATAGACGATTTTGCCGTTTTAT
ATACTCCAGGTATAGCAGCAGTATCCAAGGAAATTGCGAAAAACAAAGAATTATCTTTCCATTATACTTATAGGTGGAAT
GCTATCGCTATAGTAACTGATGGTACAAGAGTTTTAGGATTAGGAGATATTGGACCAGAGGCCGCAATGCCAGTAATGGA
AGGAAAGGCTTTGATCTTTAAATATTTAGGTGGTGTTGACGCGATACCGTTGCCGATAGGTACGAAGGATCCGGATAAAA
TTATTGAAACAGTTAAAATACTAGAACCAGCTTTTGGAGGGATTAATTTAGAGGATATAGAATCACCTAAATGTTTTTAT
ATTCTAGATAAATTAAGGCAAACAGTTGAAATTCCTGTATGGCATGACGATCAACAAGGTACAGCAGGAGCTACACTAGC
TGGATTAATAACATCTCTTGAGATAACTGGCAAAGACCCAAAAAATATAAGAGTCATTCTATATGGCGCTGGGGCAGCAA
ATATAGCTACAGCGAGATTATTATATAAATATGGTGTTCCCTATGAAAATATGATTGTTATAGATAGTAAAGGACCATTG
TATAAAGGAAGAAATGATGAGGATTCAATGAAATTAGAAAATCCATGGAAATATGAACTTCTAGAAAAAACAAATAAAAT
TGGTGCTAGGACAATAGAAGATGCTTTTAAAGGTGCTGATGTTTTAATTGCAGCTTCTAAGCCAGGCCCTGGTGTAATAA
AATCAGATTGGATAAAATTAATGAATAAAGATCCAATAGTGTTTGCATTAGCTAATCCTGTTCCTGAAATCTGGCCAGAT
GAAGCGAAAAATGCTGGTGCTAGGATTGTAGCTACTGGAAGAAGTGATTTACCTAACCAAGTAAATAACTCCTTAATATT
TCCAGCAGTTTTTAGGGGTGCTTTAGATGTTAGAGCGAAAACTATAACTGATGAAATGGTAATCGAAGCGGCAAGAGAAT
TAGCTTCCTTTGCTAGAGAAAAAGGAATTAATGAAAATTATATTATTCCCAAAATGACTGAGTGGGAAGTATACCCAAGA
GTAGCAGCTGCTGTAGGGATGAAGGCTATACAGCAAGGTATTGCAAGAGTTTCATTATCATATGACGAAATTTATTCTTC
CGCAAGAGAGATTATAAATTCTGCAAGAAAACTCCTTTCTTCATTAACATAA

Upstream 100 bases:

>100_bases
ACATGGCCTAAAACCACTACAATAGGTTGTCTAAGTCTTCTACTTTGTAACTGTTGAGACATAATCTTTCACCAACTAAA
ACCACATTGATATCTATTTC

Downstream 100 bases:

>100_bases
TTTTTTTAAGTGGTCGAAACTACTTACATATTACGACTTAGTTTGGTGAATATAATGCGTTCTTCATCAAAAATTAAAAT
AGATCCTAGTCAGCATGAGC

Product: malate oxidoreductase

Products: NA

Alternate protein names: NAD-ME [H]

Number of amino acids: Translated: 443; Mature: 443

Protein sequence:

>443_residues
MLKKFNLIFSKDTGVITLSELDKKAIEISSKYKGKIQILPKVPIKSIDDFAVLYTPGIAAVSKEIAKNKELSFHYTYRWN
AIAIVTDGTRVLGLGDIGPEAAMPVMEGKALIFKYLGGVDAIPLPIGTKDPDKIIETVKILEPAFGGINLEDIESPKCFY
ILDKLRQTVEIPVWHDDQQGTAGATLAGLITSLEITGKDPKNIRVILYGAGAANIATARLLYKYGVPYENMIVIDSKGPL
YKGRNDEDSMKLENPWKYELLEKTNKIGARTIEDAFKGADVLIAASKPGPGVIKSDWIKLMNKDPIVFALANPVPEIWPD
EAKNAGARIVATGRSDLPNQVNNSLIFPAVFRGALDVRAKTITDEMVIEAARELASFAREKGINENYIIPKMTEWEVYPR
VAAAVGMKAIQQGIARVSLSYDEIYSSAREIINSARKLLSSLT

Sequences:

>Translated_443_residues
MLKKFNLIFSKDTGVITLSELDKKAIEISSKYKGKIQILPKVPIKSIDDFAVLYTPGIAAVSKEIAKNKELSFHYTYRWN
AIAIVTDGTRVLGLGDIGPEAAMPVMEGKALIFKYLGGVDAIPLPIGTKDPDKIIETVKILEPAFGGINLEDIESPKCFY
ILDKLRQTVEIPVWHDDQQGTAGATLAGLITSLEITGKDPKNIRVILYGAGAANIATARLLYKYGVPYENMIVIDSKGPL
YKGRNDEDSMKLENPWKYELLEKTNKIGARTIEDAFKGADVLIAASKPGPGVIKSDWIKLMNKDPIVFALANPVPEIWPD
EAKNAGARIVATGRSDLPNQVNNSLIFPAVFRGALDVRAKTITDEMVIEAARELASFAREKGINENYIIPKMTEWEVYPR
VAAAVGMKAIQQGIARVSLSYDEIYSSAREIINSARKLLSSLT
>Mature_443_residues
MLKKFNLIFSKDTGVITLSELDKKAIEISSKYKGKIQILPKVPIKSIDDFAVLYTPGIAAVSKEIAKNKELSFHYTYRWN
AIAIVTDGTRVLGLGDIGPEAAMPVMEGKALIFKYLGGVDAIPLPIGTKDPDKIIETVKILEPAFGGINLEDIESPKCFY
ILDKLRQTVEIPVWHDDQQGTAGATLAGLITSLEITGKDPKNIRVILYGAGAANIATARLLYKYGVPYENMIVIDSKGPL
YKGRNDEDSMKLENPWKYELLEKTNKIGARTIEDAFKGADVLIAASKPGPGVIKSDWIKLMNKDPIVFALANPVPEIWPD
EAKNAGARIVATGRSDLPNQVNNSLIFPAVFRGALDVRAKTITDEMVIEAARELASFAREKGINENYIIPKMTEWEVYPR
VAAAVGMKAIQQGIARVSLSYDEIYSSAREIINSARKLLSSLT

Specific function: In addition to the NAD-dependent oxidative decarboxylation of L-malate, the enzyme catalyzes the decarboxylation of oxaloacetate [H]

COG id: COG0281

COG function: function code C; Malic enzyme

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the malic enzymes family [H]

Homologues:

Organism=Homo sapiens, GI4505143, Length=391, Percent_Identity=28.3887468030691, Blast_Score=104, Evalue=2e-22,
Organism=Homo sapiens, GI62420882, Length=396, Percent_Identity=28.030303030303, Blast_Score=100, Evalue=5e-21,
Organism=Homo sapiens, GI62420880, Length=396, Percent_Identity=28.030303030303, Blast_Score=100, Evalue=5e-21,
Organism=Homo sapiens, GI239049447, Length=396, Percent_Identity=28.030303030303, Blast_Score=100, Evalue=5e-21,
Organism=Escherichia coli, GI1788806, Length=407, Percent_Identity=42.2604422604423, Blast_Score=316, Evalue=2e-87,
Organism=Escherichia coli, GI87081919, Length=446, Percent_Identity=25.7847533632287, Blast_Score=93, Evalue=3e-20,
Organism=Caenorhabditis elegans, GI17537199, Length=416, Percent_Identity=28.8461538461538, Blast_Score=86, Evalue=3e-17,
Organism=Saccharomyces cerevisiae, GI6322823, Length=390, Percent_Identity=25.8974358974359, Blast_Score=93, Evalue=7e-20,
Organism=Drosophila melanogaster, GI21356279, Length=390, Percent_Identity=30, Blast_Score=110, Evalue=2e-24,
Organism=Drosophila melanogaster, GI281362672, Length=390, Percent_Identity=30, Blast_Score=110, Evalue=2e-24,
Organism=Drosophila melanogaster, GI281362674, Length=390, Percent_Identity=30, Blast_Score=110, Evalue=3e-24,
Organism=Drosophila melanogaster, GI78707242, Length=444, Percent_Identity=25, Blast_Score=89, Evalue=5e-18,
Organism=Drosophila melanogaster, GI78707238, Length=444, Percent_Identity=25, Blast_Score=89, Evalue=5e-18,
Organism=Drosophila melanogaster, GI78707240, Length=433, Percent_Identity=25.1732101616628, Blast_Score=86, Evalue=4e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015884
- InterPro:   IPR012301
- InterPro:   IPR012302
- InterPro:   IPR001891
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00390 malic; PF03949 Malic_M [H]

EC number: =1.1.1.38 [H]

Molecular weight: Translated: 48641; Mature: 48641

Theoretical pI: Translated: 8.67; Mature: 8.67

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLKKFNLIFSKDTGVITLSELDKKAIEISSKYKGKIQILPKVPIKSIDDFAVLYTPGIAA
CCCEEEEEEECCCCEEEHHHHCHHHHHHHCCCCCEEEEEECCCCCCCCCEEEEECCCHHH
VSKEIAKNKELSFHYTYRWNAIAIVTDGTRVLGLGDIGPEAAMPVMEGKALIFKYLGGVD
HHHHHHCCCCEEEEEEEEEEEEEEEECCCEEEEECCCCCHHCCCHHCCHHHHHHHHCCCC
AIPLPIGTKDPDKIIETVKILEPAFGGINLEDIESPKCFYILDKLRQTVEIPVWHDDQQG
EEECCCCCCCHHHHHHHHHHHHHCCCCCCHHHCCCCCHHHHHHHHHHHEECCEEECCCCC
TAGATLAGLITSLEITGKDPKNIRVILYGAGAANIATARLLYKYGVPYENMIVIDSKGPL
CCHHHHHHHHEEEEECCCCCCCEEEEEEECCCCHHHHHHHHHHCCCCCCCEEEECCCCCC
YKGRNDEDSMKLENPWKYELLEKTNKIGARTIEDAFKGADVLIAASKPGPGVIKSDWIKL
CCCCCCCCCCCCCCCCHHHHHHHHHHCCHHHHHHHHCCCEEEEEECCCCCCCHHHHHHHH
MNKDPIVFALANPVPEIWPDEAKNAGARIVATGRSDLPNQVNNSLIFPAVFRGALDVRAK
HCCCCEEEEECCCCCCCCCCCCCCCCCEEEEECCCCCCHHHCCCEECHHHHHCHHHHHHH
TITDEMVIEAARELASFAREKGINENYIIPKMTEWEVYPRVAAAVGMKAIQQGIARVSLS
HHHHHHHHHHHHHHHHHHHHCCCCCCEECCCCCCCHHHHHHHHHHHHHHHHHHHHHEECC
YDEIYSSAREIINSARKLLSSLT
HHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MLKKFNLIFSKDTGVITLSELDKKAIEISSKYKGKIQILPKVPIKSIDDFAVLYTPGIAA
CCCEEEEEEECCCCEEEHHHHCHHHHHHHCCCCCEEEEEECCCCCCCCCEEEEECCCHHH
VSKEIAKNKELSFHYTYRWNAIAIVTDGTRVLGLGDIGPEAAMPVMEGKALIFKYLGGVD
HHHHHHCCCCEEEEEEEEEEEEEEEECCCEEEEECCCCCHHCCCHHCCHHHHHHHHCCCC
AIPLPIGTKDPDKIIETVKILEPAFGGINLEDIESPKCFYILDKLRQTVEIPVWHDDQQG
EEECCCCCCCHHHHHHHHHHHHHCCCCCCHHHCCCCCHHHHHHHHHHHEECCEEECCCCC
TAGATLAGLITSLEITGKDPKNIRVILYGAGAANIATARLLYKYGVPYENMIVIDSKGPL
CCHHHHHHHHEEEEECCCCCCCEEEEEEECCCCHHHHHHHHHHCCCCCCCEEEECCCCCC
YKGRNDEDSMKLENPWKYELLEKTNKIGARTIEDAFKGADVLIAASKPGPGVIKSDWIKL
CCCCCCCCCCCCCCCCHHHHHHHHHHCCHHHHHHHHCCCEEEEEECCCCCCCHHHHHHHH
MNKDPIVFALANPVPEIWPDEAKNAGARIVATGRSDLPNQVNNSLIFPAVFRGALDVRAK
HCCCCEEEEECCCCCCCCCCCCCCCCCEEEEECCCCCCHHHCCCEECHHHHHCHHHHHHH
TITDEMVIEAARELASFAREKGINENYIIPKMTEWEVYPRVAAAVGMKAIQQGIARVSLS
HHHHHHHHHHHHHHHHHHHHCCCCCCEECCCCCCCHHHHHHHHHHHHHHHHHHHHHEECC
YDEIYSSAREIINSARKLLSSLT
HHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2644282 [H]