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The map label for this gene is glnA [H]

Identifier: 15920333

GI number: 15920333

Start: 180135

End: 181586

Strand: Reverse

Name: glnA [H]

Synonym: ST0156

Alternate gene names: 15920333

Gene position: 181586-180135 (Counterclockwise)

Preceding gene: 15920336

Following gene: 15920332

Centisome position: 6.74

GC content: 35.74

Gene sequence:

>1452_bases
GTGAAGTCACACATTATTCTACGTTTATTCTTACATACTTTAACTCTACCTAATGCACTAAGTAGTCCGATTTTAAGTGT
ATCCAAGGTTTATCAGAGCTCAAGACCTTCTTCATCAATCAGGTATTATACTTCATCATTGCTTATAATTTCTCATACAG
TTATTAAAAATATGAGCGTAAGTGAATTACTAAGAAAGAATAACGTAGAAATTTTACGCTTTACATGGGTAGGGTTAGAT
GGAATAATAAGATCAAAAGGGGCATACATCGATGAAGTAGACAATCTAGTAAAAACCGGAATAGGACTAACAATGGCAAT
GATGAGCTTCACACCAATGGATTACATTTCCCCTTACGGAACATTTGGTCCTCAAGATGAAGATGTATTTCTAACTCCAG
ACTTATCCACACTTTCTATTTTTCCTCCTTCCGCCGTAGTATTATGTTATCTTTATAAGGGTAGTTCACCGTGGATGTAT
GACCCCAGAAGTACTCTCAAAAAGATATTAGAAAAATACAGTGAATATGAGTTTAAATCCTCATTCGAAATAGAATTTTA
CCTAGTTAAGGATAGAAAACCTTTTGATGATACTAAATGCTTCGATTCAAGGGCTTATTACAATAATCAGATAATACCCG
AAATAGCTAAGGTTGCGGGAAGCGTAGGAATAGTTCCATTGCGTGTTATAAAAGAATACGGTCCGGGGCAATATGAATTT
GACATACAACATAAAGATGCAATGAGAAGTGCAGATGAAGTAATTCTATTTAAAGAAATTGCAAGACAGATTGCATCGAA
ATATAACGTTGAAGCAAACTTTATGCCAAAACCATTTAACAAAATGGCTGGTTCCGGGCTTCATCTTAACTTTAGTATAT
GGAAAGATGGAAGAAATTTATTTTACGATCCTAACGATAATTACGGTTTAAGTGATTTAGCTTATAATTTCATAGCTGGA
ATAATTGAACATGCAAAAGCATTAACAGCAATAGCGGCACCTACAGTAAACTCCTATAAAAGACTGGTCCCAGGATCTTG
GGCACCAACAAAAATAACTTATGGTTACAATAACAAAAGTGCAATGTTAAGAATACCCACACCATATCCTGGTATGAGTA
TGCAAGATAGAAGGATAGAGTATAGGGTACCAGATGCAACAACAAACCCCTACCTCCTATTATCGGCATTTATTGAAGCT
GGAATTGACGGTATTGAGAGGGGTCTAAAACCTCCTTCACCGGTAAACGAGAACGCGTATTTCAGAAAAGATATAGAGGA
TGTTCCCAGAAACTTGAGAGAAGCACTTAATGAGTTAAAGAAGGATACAAGGCTGATTGAAAGAGTAGGGAGAGAAATTG
TGGAGGAGTTTATCAGAGTAAAGATGGCTGAAGTTGAAGAATTCGAAAGCTTAGTAACAGATTGGGAATACGAGGTATAT
AAGTATGTTTAA

Upstream 100 bases:

>100_bases
ACAAAGCTCCAATTTTCAGCTTTCCAATACCCTTAATTTCCTCGTTCATTATTTAATTTTTGATACTTGAACAAATAAGT
TTTATGACAAGTTATAGATA

Downstream 100 bases:

>100_bases
GGTAATTGATGATCACGCTCACTGGTTTAGTGTAAAACCCCTAGATGAAAAAGGCTTAGCATTAGAATCAGCAGAAAGTT
GGATCGAAGGAGAGATTAAC

Product: glutamine synthetase

Products: NA

Alternate protein names: GS; Glutamate--ammonia ligase [H]

Number of amino acids: Translated: 483; Mature: 483

Protein sequence:

>483_residues
MKSHIILRLFLHTLTLPNALSSPILSVSKVYQSSRPSSSIRYYTSSLLIISHTVIKNMSVSELLRKNNVEILRFTWVGLD
GIIRSKGAYIDEVDNLVKTGIGLTMAMMSFTPMDYISPYGTFGPQDEDVFLTPDLSTLSIFPPSAVVLCYLYKGSSPWMY
DPRSTLKKILEKYSEYEFKSSFEIEFYLVKDRKPFDDTKCFDSRAYYNNQIIPEIAKVAGSVGIVPLRVIKEYGPGQYEF
DIQHKDAMRSADEVILFKEIARQIASKYNVEANFMPKPFNKMAGSGLHLNFSIWKDGRNLFYDPNDNYGLSDLAYNFIAG
IIEHAKALTAIAAPTVNSYKRLVPGSWAPTKITYGYNNKSAMLRIPTPYPGMSMQDRRIEYRVPDATTNPYLLLSAFIEA
GIDGIERGLKPPSPVNENAYFRKDIEDVPRNLREALNELKKDTRLIERVGREIVEEFIRVKMAEVEEFESLVTDWEYEVY
KYV

Sequences:

>Translated_483_residues
MKSHIILRLFLHTLTLPNALSSPILSVSKVYQSSRPSSSIRYYTSSLLIISHTVIKNMSVSELLRKNNVEILRFTWVGLD
GIIRSKGAYIDEVDNLVKTGIGLTMAMMSFTPMDYISPYGTFGPQDEDVFLTPDLSTLSIFPPSAVVLCYLYKGSSPWMY
DPRSTLKKILEKYSEYEFKSSFEIEFYLVKDRKPFDDTKCFDSRAYYNNQIIPEIAKVAGSVGIVPLRVIKEYGPGQYEF
DIQHKDAMRSADEVILFKEIARQIASKYNVEANFMPKPFNKMAGSGLHLNFSIWKDGRNLFYDPNDNYGLSDLAYNFIAG
IIEHAKALTAIAAPTVNSYKRLVPGSWAPTKITYGYNNKSAMLRIPTPYPGMSMQDRRIEYRVPDATTNPYLLLSAFIEA
GIDGIERGLKPPSPVNENAYFRKDIEDVPRNLREALNELKKDTRLIERVGREIVEEFIRVKMAEVEEFESLVTDWEYEVY
KYV
>Mature_483_residues
MKSHIILRLFLHTLTLPNALSSPILSVSKVYQSSRPSSSIRYYTSSLLIISHTVIKNMSVSELLRKNNVEILRFTWVGLD
GIIRSKGAYIDEVDNLVKTGIGLTMAMMSFTPMDYISPYGTFGPQDEDVFLTPDLSTLSIFPPSAVVLCYLYKGSSPWMY
DPRSTLKKILEKYSEYEFKSSFEIEFYLVKDRKPFDDTKCFDSRAYYNNQIIPEIAKVAGSVGIVPLRVIKEYGPGQYEF
DIQHKDAMRSADEVILFKEIARQIASKYNVEANFMPKPFNKMAGSGLHLNFSIWKDGRNLFYDPNDNYGLSDLAYNFIAG
IIEHAKALTAIAAPTVNSYKRLVPGSWAPTKITYGYNNKSAMLRIPTPYPGMSMQDRRIEYRVPDATTNPYLLLSAFIEA
GIDGIERGLKPPSPVNENAYFRKDIEDVPRNLREALNELKKDTRLIERVGREIVEEFIRVKMAEVEEFESLVTDWEYEVY
KYV

Specific function: Unknown

COG id: COG0174

COG function: function code E; Glutamine synthetase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glutamine synthetase family [H]

Homologues:

Organism=Homo sapiens, GI221307485, Length=428, Percent_Identity=24.2990654205607, Blast_Score=113, Evalue=3e-25,
Organism=Escherichia coli, GI1790301, Length=254, Percent_Identity=38.5826771653543, Blast_Score=166, Evalue=4e-42,
Organism=Escherichia coli, GI87081870, Length=449, Percent_Identity=25.8351893095768, Blast_Score=146, Evalue=3e-36,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1040 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 15324 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 8,000 Molecules/Cell In: Glucose minimal medi

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008147
- InterPro:   IPR014746
- InterPro:   IPR008146
- InterPro:   IPR004809 [H]

Pfam domain/function: PF00120 Gln-synt_C; PF03951 Gln-synt_N [H]

EC number: =6.3.1.2 [H]

Molecular weight: Translated: 55206; Mature: 55206

Theoretical pI: Translated: 7.14; Mature: 7.14

Prosite motif: PS00181 GLNA_ATP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKSHIILRLFLHTLTLPNALSSPILSVSKVYQSSRPSSSIRYYTSSLLIISHTVIKNMSV
CCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCCH
SELLRKNNVEILRFTWVGLDGIIRSKGAYIDEVDNLVKTGIGLTMAMMSFTPMDYISPYG
HHHHHHCCCEEEEEEEECHHHHHHCCCCCHHHHHHHHHHCHHHHHHHHHCCCHHHCCCCC
TFGPQDEDVFLTPDLSTLSIFPPSAVVLCYLYKGSSPWMYDPRSTLKKILEKYSEYEFKS
CCCCCCCCEEECCCCCEEEECCCCCEEEEEEEECCCCCCCCCHHHHHHHHHHHHCCCCCC
SFEIEFYLVKDRKPFDDTKCFDSRAYYNNQIIPEIAKVAGSVGIVPLRVIKEYGPGQYEF
CCEEEEEEEECCCCCCCHHHHCCHHHHCCCHHHHHHHHCCCCCCHHHHHHHHCCCCCEEE
DIQHKDAMRSADEVILFKEIARQIASKYNVEANFMPKPFNKMAGSGLHLNFSIWKDGRNL
EECHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHCCCCEEEEEEEEECCCEE
FYDPNDNYGLSDLAYNFIAGIIEHAKALTAIAAPTVNSYKRLVPGSWAPTKITYGYNNKS
EECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCCCCCEEEEEECCCC
AMLRIPTPYPGMSMQDRRIEYRVPDATTNPYLLLSAFIEAGIDGIERGLKPPSPVNENAY
EEEECCCCCCCCCCCCCEEEEECCCCCCCHHHHHHHHHHHCHHHHHHCCCCCCCCCCCCH
FRKDIEDVPRNLREALNELKKDTRLIERVGREIVEEFIRVKMAEVEEFESLVTDWEYEVY
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEE
KYV
ECC
>Mature Secondary Structure
MKSHIILRLFLHTLTLPNALSSPILSVSKVYQSSRPSSSIRYYTSSLLIISHTVIKNMSV
CCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCCH
SELLRKNNVEILRFTWVGLDGIIRSKGAYIDEVDNLVKTGIGLTMAMMSFTPMDYISPYG
HHHHHHCCCEEEEEEEECHHHHHHCCCCCHHHHHHHHHHCHHHHHHHHHCCCHHHCCCCC
TFGPQDEDVFLTPDLSTLSIFPPSAVVLCYLYKGSSPWMYDPRSTLKKILEKYSEYEFKS
CCCCCCCCEEECCCCCEEEECCCCCEEEEEEEECCCCCCCCCHHHHHHHHHHHHCCCCCC
SFEIEFYLVKDRKPFDDTKCFDSRAYYNNQIIPEIAKVAGSVGIVPLRVIKEYGPGQYEF
CCEEEEEEEECCCCCCCHHHHCCHHHHCCCHHHHHHHHCCCCCCHHHHHHHHCCCCCEEE
DIQHKDAMRSADEVILFKEIARQIASKYNVEANFMPKPFNKMAGSGLHLNFSIWKDGRNL
EECHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHCCCCEEEEEEEEECCCEE
FYDPNDNYGLSDLAYNFIAGIIEHAKALTAIAAPTVNSYKRLVPGSWAPTKITYGYNNKS
EECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCCCCCEEEEEECCCC
AMLRIPTPYPGMSMQDRRIEYRVPDATTNPYLLLSAFIEAGIDGIERGLKPPSPVNENAY
EEEECCCCCCCCCCCCCEEEEECCCCCCCHHHHHHHHHHHCHHHHHHCCCCCCCCCCCCH
FRKDIEDVPRNLREALNELKKDTRLIERVGREIVEEFIRVKMAEVEEFESLVTDWEYEVY
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEE
KYV
ECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]