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The map label for this gene is proP [H]

Identifier: 15920231

GI number: 15920231

Start: 70906

End: 72234

Strand: Reverse

Name: proP [H]

Synonym: ST0054

Alternate gene names: 15920231

Gene position: 72234-70906 (Counterclockwise)

Preceding gene: 15920232

Following gene: 15920230

Centisome position: 2.68

GC content: 36.79

Gene sequence:

>1329_bases
ATGAAACCAGATGATAAACTAACTAGTAAACATGCTAAATGGATATTAGCTACATTTTTAGCGTGGACGTTCGATTTGTA
TGATCTCTTTAGTATCCTACTTGTAACTCCTTATATAGCAGAACTATTCTTCCCATCAAAGGTAGTGTTTCTTGCAATAG
CTGCTACTTACGCTACCTTTTTAACATCATTTCTTATGAGACCAGTAGGGGCTCTATATTTCGGAAACAGTATTTCAGAT
AAATTAGGAAGAAAGAGAGCAATACTCTATGGTATAATAGGACTAGTTATTACTGCGAGCCTTCAAGGGGCATTACCAAC
TTATGCGGTTGTAGGAATTTTTGCTCCAATACTTATAATACTAGTAAGATTAGCGGAGGGATTCTTTGTTGGAGGCGTGA
CTGCGGGAAGTCATACTATAGGACCAGAATCGGTACCAGAAAGACATAGAGGCTGGGTAGGTGGAATAGGTTTCTCTGCT
GCTGGTGCAGCATATTTAATAGCTTCTGCTTGGTTTTACATGACTGCTCTACTATTTCCTGGCTCGTCCTATTTAGTATG
GGGCTGGAGAGTAATGTTTTTCGGTGGGCTTCTGCCGTTGGCAGTATTAGGTTTCGTTAGCTATTTAGTTCCAGAGTCAG
ATGCTTGGCAAAAAGCTAAAGACAGAGGGAAAACTATAAAATCACCAGTTAGAGAGCTTTTCTCAAAGTTCAGAAGAACA
TTTGGCATTGCCTTAGTACTTACTGTTGGTTGGGCGTTAATGTATTATTTAACACAAGGTTTATTTCCAACATTTCTAAG
TGAAATTAACGGTTTAACAAAGGCTGAAATAGGAATTACAATGATAATAGCTTCAATTGGTATGATAATAGGCCCTACAT
TAGGAGGTGAAATTTCCCAACATATAGGAAGGAAGTTAATGTCAATAATAGGAGCTGTAATAATAATTGCCATTTCTCCT
CTATATTTATATTTAGGATCTTTAACTACAAGTTCTTTAAATAATGTAATTCTAGTGACGTTTTTAATATCGTTATTAAG
CGATTTCGGAGGAGGTATGTTGATGACATATCTGAATGAGGCTTATCCTACTAGTATTAGGGGAACTGGTGTTGCATTTA
CTTGGAATACTGGTTTCGCTATAGGAGGGGCCTCTCCAACTATTATAAGCCTTGTGTTAGCGTCTATTGGTGCATCAAAG
TTCCCATCAGTTATGTTTTATGCTCTAGTGATTGTAGGGGTAATCCTATTAATATCCTCAATTATAACTAATGATACAAA
GGGTAATATTTTAAAGGAAATTGAATCAGTTGAAAAGGGAGCTATATGA

Upstream 100 bases:

>100_bases
AAAAATACTAATGATTAAATTTATTATTTTTATAATGTATTATATTCAAGTAACATTTATAAGTGAAAATAATAAACTAT
AGTTTAGAGGTTAATATAAA

Downstream 100 bases:

>100_bases
AAGCTATGTTGATTAAGGAATTTGGAAAGCCGTTAAAATTAGAAGATGTTGATGTTCCTAAGCTTAAGCAAAATCAAGTT
CTCATTAGAGTAAGAAGTGC

Product: transporter

Products: betaine [Cytoplasm]; Proton [Cytoplasm]; L-proline [Cytoplasm] [C]

Alternate protein names: Proline porter II; PPII [H]

Number of amino acids: Translated: 442; Mature: 442

Protein sequence:

>442_residues
MKPDDKLTSKHAKWILATFLAWTFDLYDLFSILLVTPYIAELFFPSKVVFLAIAATYATFLTSFLMRPVGALYFGNSISD
KLGRKRAILYGIIGLVITASLQGALPTYAVVGIFAPILIILVRLAEGFFVGGVTAGSHTIGPESVPERHRGWVGGIGFSA
AGAAYLIASAWFYMTALLFPGSSYLVWGWRVMFFGGLLPLAVLGFVSYLVPESDAWQKAKDRGKTIKSPVRELFSKFRRT
FGIALVLTVGWALMYYLTQGLFPTFLSEINGLTKAEIGITMIIASIGMIIGPTLGGEISQHIGRKLMSIIGAVIIIAISP
LYLYLGSLTTSSLNNVILVTFLISLLSDFGGGMLMTYLNEAYPTSIRGTGVAFTWNTGFAIGGASPTIISLVLASIGASK
FPSVMFYALVIVGVILLISSIITNDTKGNILKEIESVEKGAI

Sequences:

>Translated_442_residues
MKPDDKLTSKHAKWILATFLAWTFDLYDLFSILLVTPYIAELFFPSKVVFLAIAATYATFLTSFLMRPVGALYFGNSISD
KLGRKRAILYGIIGLVITASLQGALPTYAVVGIFAPILIILVRLAEGFFVGGVTAGSHTIGPESVPERHRGWVGGIGFSA
AGAAYLIASAWFYMTALLFPGSSYLVWGWRVMFFGGLLPLAVLGFVSYLVPESDAWQKAKDRGKTIKSPVRELFSKFRRT
FGIALVLTVGWALMYYLTQGLFPTFLSEINGLTKAEIGITMIIASIGMIIGPTLGGEISQHIGRKLMSIIGAVIIIAISP
LYLYLGSLTTSSLNNVILVTFLISLLSDFGGGMLMTYLNEAYPTSIRGTGVAFTWNTGFAIGGASPTIISLVLASIGASK
FPSVMFYALVIVGVILLISSIITNDTKGNILKEIESVEKGAI
>Mature_442_residues
MKPDDKLTSKHAKWILATFLAWTFDLYDLFSILLVTPYIAELFFPSKVVFLAIAATYATFLTSFLMRPVGALYFGNSISD
KLGRKRAILYGIIGLVITASLQGALPTYAVVGIFAPILIILVRLAEGFFVGGVTAGSHTIGPESVPERHRGWVGGIGFSA
AGAAYLIASAWFYMTALLFPGSSYLVWGWRVMFFGGLLPLAVLGFVSYLVPESDAWQKAKDRGKTIKSPVRELFSKFRRT
FGIALVLTVGWALMYYLTQGLFPTFLSEINGLTKAEIGITMIIASIGMIIGPTLGGEISQHIGRKLMSIIGAVIIIAISP
LYLYLGSLTTSSLNNVILVTFLISLLSDFGGGMLMTYLNEAYPTSIRGTGVAFTWNTGFAIGGASPTIISLVLASIGASK
FPSVMFYALVIVGVILLISSIITNDTKGNILKEIESVEKGAI

Specific function: Proton symporter that senses osmotic shifts and responds by importing osmolytes such as proline, glycine betaine, stachydrine, pipecolic acid, ectoine and taurine. It is both an osmosensor and an osmoregulator which is available to participate early in th

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family [H]

Homologues:

Organism=Escherichia coli, GI1790550, Length=439, Percent_Identity=26.879271070615, Blast_Score=148, Evalue=6e-37,
Organism=Escherichia coli, GI1788292, Length=439, Percent_Identity=23.4624145785877, Blast_Score=100, Evalue=2e-22,
Organism=Escherichia coli, GI1789941, Length=441, Percent_Identity=27.6643990929705, Blast_Score=99, Evalue=7e-22,
Organism=Escherichia coli, GI1788942, Length=435, Percent_Identity=25.7471264367816, Blast_Score=96, Evalue=7e-21,
Organism=Escherichia coli, GI87082404, Length=420, Percent_Identity=24.5238095238095, Blast_Score=87, Evalue=2e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004736
- InterPro:   IPR020846
- InterPro:   IPR016196
- InterPro:   IPR015041
- InterPro:   IPR005828
- InterPro:   IPR005829 [H]

Pfam domain/function: PF08946 Osmo_CC; PF00083 Sugar_tr [H]

EC number: NA

Molecular weight: Translated: 47603; Mature: 47603

Theoretical pI: Translated: 9.86; Mature: 9.86

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHCCCCCHHHHHHHHHHHCCCC
>Mature Secondary Structure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HHCCCCCHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: betaine [Periplasm]; Proton [Periplasm]; L-proline [Periplasm] [C]

Specific reaction: Proton [Periplasm] + betaine [Periplasm] = Proton [Cytoplasm] + betaine [Cytoplasm] Proton [Periplasm] + L-proline [Periplasm] = Proton [Cytoplasm] + L-proline [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]