BacMap

Definition	Sulfolobus tokodaii str. 7 chromosome, complete genome.
Accession	NC_003106
Length	2,694,756

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The map label for this gene is paaF [C]

Identifier: 15920225

GI number: 15920225

Start: 64646

End: 65410

Strand: Reverse

Name: paaF [C]

Synonym: ST0048

Alternate gene names: 15920225

Gene position: 65410-64646 (Counterclockwise)

Preceding gene: 15920227

Following gene: 15920224

Centisome position: 2.43

GC content: 34.9

Gene sequence:

>765_bases
ATGATTAAGGTTGAGGTAAATAAAGAGAATAAAATAGGTAAAATATTAATAGATAGACAAGAAAAAATGAACGCAATAAC
CGTTGAGATGAGAAAAGAAATAGGAGAGAAAATAATAGAATTAGACAAAGATCCGAGCGTAAGAGTAATAATTATAGAAG
GTGTAGGTGGAAAAGCATTTAGTTCTGGAGGAGATATAAGTGAATTCCTATCATTAAACCCAGAGACTTTACTAGATTGG
GGAGAAGATCTATCAACAAGTGAAAGAATAACTAAGCCAGTAATAGCTGCAATAAACGGATACACATTTGGTGCAGGCTT
AGAATTAGCATTATCTTGTGATATAAGAATAGCCACACCTAAAAGTGAATTTAGCTTTCCAGAAATAAGACTAGGAATGG
TACCTGCAAGTGGAGGAGTAACTAGGATAGTAAAAATGTTAGGTGTATCTAGAGCTACTTATATGCTAATGTTAGGAAAG
AGAATAGACGCACAAACTGCTCTTCAGTGGGGTATAGTACATGAGATTGTTGATGAAGATAAACTAGAAGGAAGAGCATT
AGAAATAGCTAAAGATCTAGCTAGCCTATCACCCTTAGCCCTCAAAGCGTTAAAGAAAATAATAAGAGAGGTTGCAGATT
CACCATTTTACGCAGGATTTGATATTGAAAGGAAAACTTTCGGCTTACTTAGATATAGTGAAGACTTCAGAGAAGGCGTA
GAGTCCTTCTTAAATAAGAGGAAGCCTAATTTTAACGGTAGGTAA

Upstream 100 bases:

>100_bases
CCAGAAATTATAAAGAAATTAGAAAAACAATAAAAATATTACTAAGTTAAGGGAAAAACTTAAATAATATTATGTACAGT
TAAGATTAGTGTGATATTAT

Downstream 100 bases:

>100_bases
GTATGATATTTAAAGAGGGGTTTCGATTTGTAAAGGGAGAAGGTTCGTATATTGATGACGTATTAACTAAAGAAATAAAA
GGTTATTTTTCTGTTTCTAT

Product: enoyl-CoA hydratase

Products: NA

Alternate protein names: 3-hydroxypropionyl-CoA dehydratase [H]

Number of amino acids: Translated: 254; Mature: 254

Protein sequence:

>254_residues
MIKVEVNKENKIGKILIDRQEKMNAITVEMRKEIGEKIIELDKDPSVRVIIIEGVGGKAFSSGGDISEFLSLNPETLLDW
GEDLSTSERITKPVIAAINGYTFGAGLELALSCDIRIATPKSEFSFPEIRLGMVPASGGVTRIVKMLGVSRATYMLMLGK
RIDAQTALQWGIVHEIVDEDKLEGRALEIAKDLASLSPLALKALKKIIREVADSPFYAGFDIERKTFGLLRYSEDFREGV
ESFLNKRKPNFNGR

Sequences:

>Translated_254_residues
MIKVEVNKENKIGKILIDRQEKMNAITVEMRKEIGEKIIELDKDPSVRVIIIEGVGGKAFSSGGDISEFLSLNPETLLDW
GEDLSTSERITKPVIAAINGYTFGAGLELALSCDIRIATPKSEFSFPEIRLGMVPASGGVTRIVKMLGVSRATYMLMLGK
RIDAQTALQWGIVHEIVDEDKLEGRALEIAKDLASLSPLALKALKKIIREVADSPFYAGFDIERKTFGLLRYSEDFREGV
ESFLNKRKPNFNGR
>Mature_254_residues
MIKVEVNKENKIGKILIDRQEKMNAITVEMRKEIGEKIIELDKDPSVRVIIIEGVGGKAFSSGGDISEFLSLNPETLLDW
GEDLSTSERITKPVIAAINGYTFGAGLELALSCDIRIATPKSEFSFPEIRLGMVPASGGVTRIVKMLGVSRATYMLMLGK
RIDAQTALQWGIVHEIVDEDKLEGRALEIAKDLASLSPLALKALKKIIREVADSPFYAGFDIERKTFGLLRYSEDFREGV
ESFLNKRKPNFNGR

Specific function: Plays a role in autotrophic carbon fixation via the 3- hydroxypropionate/4-hydroxybutyrate cycle. Catalyzes the reversible dehydration of 3-hydroxypropionyl-CoA to form acryloyl- CoA, and the reversible dehydration of (S)-3-hydroxybutyryl-CoA to form crot

COG id: COG1024

COG function: function code I; Enoyl-CoA hydratase/carnithine racemase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family [H]

Homologues:

Organism=Homo sapiens, GI194097323, Length=249, Percent_Identity=34.9397590361446, Blast_Score=152, Evalue=2e-37,
Organism=Homo sapiens, GI68989263, Length=166, Percent_Identity=37.3493975903614, Blast_Score=105, Evalue=3e-23,
Organism=Homo sapiens, GI31542718, Length=254, Percent_Identity=29.9212598425197, Blast_Score=96, Evalue=3e-20,
Organism=Homo sapiens, GI157694516, Length=214, Percent_Identity=30.8411214953271, Blast_Score=96, Evalue=3e-20,
Organism=Homo sapiens, GI213417737, Length=214, Percent_Identity=30.8411214953271, Blast_Score=96, Evalue=3e-20,
Organism=Homo sapiens, GI20127408, Length=166, Percent_Identity=33.7349397590361, Blast_Score=92, Evalue=6e-19,
Organism=Homo sapiens, GI45643119, Length=250, Percent_Identity=27.2, Blast_Score=88, Evalue=7e-18,
Organism=Homo sapiens, GI260275230, Length=250, Percent_Identity=27.2, Blast_Score=88, Evalue=7e-18,
Organism=Homo sapiens, GI37594469, Length=192, Percent_Identity=29.1666666666667, Blast_Score=88, Evalue=8e-18,
Organism=Homo sapiens, GI260274832, Length=250, Percent_Identity=27.2, Blast_Score=88, Evalue=8e-18,
Organism=Homo sapiens, GI37594471, Length=193, Percent_Identity=31.6062176165803, Blast_Score=88, Evalue=9e-18,
Organism=Homo sapiens, GI157694520, Length=187, Percent_Identity=31.0160427807487, Blast_Score=85, Evalue=8e-17,
Organism=Homo sapiens, GI4502327, Length=246, Percent_Identity=26.4227642276423, Blast_Score=83, Evalue=3e-16,
Organism=Homo sapiens, GI70995211, Length=214, Percent_Identity=26.6355140186916, Blast_Score=77, Evalue=2e-14,
Organism=Homo sapiens, GI116235487, Length=221, Percent_Identity=27.1493212669683, Blast_Score=73, Evalue=3e-13,
Organism=Homo sapiens, GI25453481, Length=213, Percent_Identity=26.7605633802817, Blast_Score=70, Evalue=2e-12,
Organism=Homo sapiens, GI51538573, Length=213, Percent_Identity=26.7605633802817, Blast_Score=70, Evalue=2e-12,
Organism=Homo sapiens, GI4757966, Length=213, Percent_Identity=26.7605633802817, Blast_Score=69, Evalue=3e-12,
Organism=Homo sapiens, GI51538581, Length=213, Percent_Identity=26.7605633802817, Blast_Score=69, Evalue=3e-12,
Organism=Homo sapiens, GI261878539, Length=82, Percent_Identity=39.0243902439024, Blast_Score=67, Evalue=1e-11,
Organism=Homo sapiens, GI221136756, Length=216, Percent_Identity=24.0740740740741, Blast_Score=65, Evalue=6e-11,
Organism=Homo sapiens, GI221136753, Length=216, Percent_Identity=24.0740740740741, Blast_Score=65, Evalue=6e-11,
Organism=Homo sapiens, GI221307494, Length=216, Percent_Identity=24.0740740740741, Blast_Score=65, Evalue=8e-11,
Organism=Escherichia coli, GI1787659, Length=256, Percent_Identity=35.15625, Blast_Score=163, Evalue=1e-41,
Organism=Escherichia coli, GI221142681, Length=258, Percent_Identity=37.5968992248062, Blast_Score=159, Evalue=2e-40,
Organism=Escherichia coli, GI1787660, Length=258, Percent_Identity=29.8449612403101, Blast_Score=114, Evalue=8e-27,
Organism=Escherichia coli, GI87082183, Length=258, Percent_Identity=29.4573643410853, Blast_Score=108, Evalue=5e-25,
Organism=Escherichia coli, GI1788597, Length=251, Percent_Identity=29.0836653386454, Blast_Score=103, Evalue=9e-24,
Organism=Escherichia coli, GI1788682, Length=202, Percent_Identity=33.6633663366337, Blast_Score=100, Evalue=8e-23,
Organism=Escherichia coli, GI1790281, Length=181, Percent_Identity=33.7016574585635, Blast_Score=97, Evalue=8e-22,
Organism=Caenorhabditis elegans, GI17554946, Length=258, Percent_Identity=33.3333333333333, Blast_Score=146, Evalue=1e-35,
Organism=Caenorhabditis elegans, GI25145438, Length=243, Percent_Identity=33.7448559670782, Blast_Score=142, Evalue=1e-34,
Organism=Caenorhabditis elegans, GI17540714, Length=232, Percent_Identity=28.8793103448276, Blast_Score=122, Evalue=2e-28,
Organism=Caenorhabditis elegans, GI17534483, Length=229, Percent_Identity=31.0043668122271, Blast_Score=106, Evalue=1e-23,
Organism=Caenorhabditis elegans, GI17560910, Length=253, Percent_Identity=27.2727272727273, Blast_Score=103, Evalue=9e-23,
Organism=Caenorhabditis elegans, GI17536985, Length=261, Percent_Identity=28.3524904214559, Blast_Score=98, Evalue=4e-21,
Organism=Caenorhabditis elegans, GI17540306, Length=266, Percent_Identity=27.0676691729323, Blast_Score=96, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI17535521, Length=254, Percent_Identity=26.7716535433071, Blast_Score=95, Evalue=3e-20,
Organism=Caenorhabditis elegans, GI17558304, Length=162, Percent_Identity=33.9506172839506, Blast_Score=89, Evalue=3e-18,
Organism=Caenorhabditis elegans, GI17508953, Length=180, Percent_Identity=33.3333333333333, Blast_Score=79, Evalue=3e-15,
Organism=Caenorhabditis elegans, GI25144276, Length=180, Percent_Identity=33.3333333333333, Blast_Score=79, Evalue=3e-15,
Organism=Caenorhabditis elegans, GI17508951, Length=180, Percent_Identity=33.3333333333333, Blast_Score=78, Evalue=3e-15,
Organism=Caenorhabditis elegans, GI25141363, Length=175, Percent_Identity=29.7142857142857, Blast_Score=72, Evalue=3e-13,
Organism=Caenorhabditis elegans, GI25144160, Length=220, Percent_Identity=24.0909090909091, Blast_Score=71, Evalue=5e-13,
Organism=Caenorhabditis elegans, GI25144157, Length=220, Percent_Identity=24.0909090909091, Blast_Score=71, Evalue=5e-13,
Organism=Caenorhabditis elegans, GI17549921, Length=204, Percent_Identity=23.5294117647059, Blast_Score=70, Evalue=1e-12,
Organism=Saccharomyces cerevisiae, GI6320241, Length=198, Percent_Identity=23.7373737373737, Blast_Score=69, Evalue=1e-12,
Organism=Drosophila melanogaster, GI20129971, Length=257, Percent_Identity=33.852140077821, Blast_Score=154, Evalue=5e-38,
Organism=Drosophila melanogaster, GI24653477, Length=257, Percent_Identity=33.852140077821, Blast_Score=154, Evalue=5e-38,
Organism=Drosophila melanogaster, GI24653139, Length=250, Percent_Identity=26, Blast_Score=109, Evalue=1e-24,
Organism=Drosophila melanogaster, GI21357171, Length=258, Percent_Identity=30.2325581395349, Blast_Score=98, Evalue=7e-21,
Organism=Drosophila melanogaster, GI24650670, Length=250, Percent_Identity=27.2, Blast_Score=96, Evalue=3e-20,
Organism=Drosophila melanogaster, GI19920382, Length=255, Percent_Identity=29.8039215686275, Blast_Score=94, Evalue=8e-20,
Organism=Drosophila melanogaster, GI19922422, Length=263, Percent_Identity=24.7148288973384, Blast_Score=86, Evalue=2e-17,
Organism=Drosophila melanogaster, GI19921000, Length=195, Percent_Identity=34.8717948717949, Blast_Score=81, Evalue=8e-16,
Organism=Drosophila melanogaster, GI24583077, Length=195, Percent_Identity=34.8717948717949, Blast_Score=80, Evalue=9e-16,
Organism=Drosophila melanogaster, GI24583079, Length=195, Percent_Identity=34.8717948717949, Blast_Score=80, Evalue=9e-16,
Organism=Drosophila melanogaster, GI28571730, Length=175, Percent_Identity=29.7142857142857, Blast_Score=80, Evalue=1e-15,
Organism=Drosophila melanogaster, GI28571729, Length=175, Percent_Identity=29.7142857142857, Blast_Score=80, Evalue=1e-15,
Organism=Drosophila melanogaster, GI24654903, Length=209, Percent_Identity=25.3588516746411, Blast_Score=70, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014748
- InterPro:   IPR001753
- InterPro:   IPR018376 [H]

Pfam domain/function: PF00378 ECH [H]

EC number: =4.2.1.116 [H]

Molecular weight: Translated: 28177; Mature: 28177

Theoretical pI: Translated: 6.13; Mature: 6.13

Prosite motif: PS00166 ENOYL_COA_HYDRATASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIKVEVNKENKIGKILIDRQEKMNAITVEMRKEIGEKIIELDKDPSVRVIIIEGVGGKAF
CEEEEECCCCCCCCEEECCHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCC
SSGGDISEFLSLNPETLLDWGEDLSTSERITKPVIAAINGYTFGAGLELALSCDIRIATP
CCCCCHHHHHHCCHHHHHHCCCCCCCHHHHHHHHHHHHCCEEECCCCEEEEEEEEEEECC
KSEFSFPEIRLGMVPASGGVTRIVKMLGVSRATYMLMLGKRIDAQTALQWGIVHEIVDED
CCCCCCCCEEEEEEECCCCHHHHHHHHCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCC
KLEGRALEIAKDLASLSPLALKALKKIIREVADSPFYAGFDIERKTFGLLRYSEDFREGV
CCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCEECCCCCHHHHHHHHHHHHHHHHH
ESFLNKRKPNFNGR
HHHHHHCCCCCCCC
>Mature Secondary Structure
MIKVEVNKENKIGKILIDRQEKMNAITVEMRKEIGEKIIELDKDPSVRVIIIEGVGGKAF
CEEEEECCCCCCCCEEECCHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCC
SSGGDISEFLSLNPETLLDWGEDLSTSERITKPVIAAINGYTFGAGLELALSCDIRIATP
CCCCCHHHHHHCCHHHHHHCCCCCCCHHHHHHHHHHHHCCEEECCCCEEEEEEEEEEECC
KSEFSFPEIRLGMVPASGGVTRIVKMLGVSRATYMLMLGKRIDAQTALQWGIVHEIVDED
CCCCCCCCEEEEEEECCCCHHHHHHHHCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCC
KLEGRALEIAKDLASLSPLALKALKKIIREVADSPFYAGFDIERKTFGLLRYSEDFREGV
CCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCEECCCCCHHHHHHHHHHHHHHHHH
ESFLNKRKPNFNGR
HHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA