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The map label for this gene is rhtB [H]

Identifier: 159185318

GI number: 159185318

Start: 2653761

End: 2654408

Strand: Direct

Name: rhtB [H]

Synonym: Atu2669

Alternate gene names: 159185318

Gene position: 2653761-2654408 (Clockwise)

Preceding gene: 159185317

Following gene: 159185323

Centisome position: 93.39

GC content: 58.18

Gene sequence:

>648_bases
ATGGATTTCGTTCCGAGCCTGCCAACATTGATCGCCTTCACCATTGCCATTCTGCTTCTGGCGGTGACGCCGGGTCCTGA
CATGACGCTCTGGATCAGCCGCTCGCTGCGCGAAGGCCGGGCGGCGGGCTTCATGACGCTGGTCGGAACCAATATCGGCA
TCACCGTGCATACGATGCTGGTCGCTTTTGGTGTCGCGGCTCTCATCGTCGCCTCGCCGACCGCCTTCATGATCCTGAAA
ACCGGTGGTGCGGCCTATCTGGTCTGGCTCGCCATACAGGCAATCCGCAAGGGCTCGGATTTCGTGATGGTGAAAAGCAC
CGGTGAAAAAGCACAGGCCTCCCTGAAATCGGCCTTGCTGAACGGCATCTGGGTCAATCTGCTGAACCCCAAGGTCATCA
TCTTTTTCATGACCTTCCTGCCGCAATTTGTCAGCGCAACCGATCCGCACGTCACCGGCAAGCTGATCTTCCTCGGCATC
TGGTCCATCATCGTGGCGCTGCCGATCGGCATCGGCATCGTTGTCACCGCCGATATTCTTTCCGCCTGGCTGCAGCGCAA
CCGGAAAGTTCTGCGCGGACTCGATTATACGATCGCCGGCGTCTTCTCGCTTTTCGCGGTCAAGATTTTCTTCACGCAAA
CGCGCTGA

Upstream 100 bases:

>100_bases
GGCTATTTCCCCCTCGCAGGCGCGTCATCGCTCAAAAACATTGGCAGAAACACGAAACCGCGTTAAGCAACCGCATTCTC
CAGATTTTACGGGTGCCGCG

Downstream 100 bases:

>100_bases
GGGCAAATAAAAAGGCGCCCAAAGGCGCCTTTTTGCAATCAGCCGATCAGCAGTTCGTCGTCGTCAAGCGTCTGGCCGCG
TATCTTGCGGAACATCTCGA

Product: RhtB family transporter

Products: homoserine lactone [Periplasm]; Proton [Cytoplasm]; homoserine [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 215; Mature: 215

Protein sequence:

>215_residues
MDFVPSLPTLIAFTIAILLLAVTPGPDMTLWISRSLREGRAAGFMTLVGTNIGITVHTMLVAFGVAALIVASPTAFMILK
TGGAAYLVWLAIQAIRKGSDFVMVKSTGEKAQASLKSALLNGIWVNLLNPKVIIFFMTFLPQFVSATDPHVTGKLIFLGI
WSIIVALPIGIGIVVTADILSAWLQRNRKVLRGLDYTIAGVFSLFAVKIFFTQTR

Sequences:

>Translated_215_residues
MDFVPSLPTLIAFTIAILLLAVTPGPDMTLWISRSLREGRAAGFMTLVGTNIGITVHTMLVAFGVAALIVASPTAFMILK
TGGAAYLVWLAIQAIRKGSDFVMVKSTGEKAQASLKSALLNGIWVNLLNPKVIIFFMTFLPQFVSATDPHVTGKLIFLGI
WSIIVALPIGIGIVVTADILSAWLQRNRKVLRGLDYTIAGVFSLFAVKIFFTQTR
>Mature_215_residues
MDFVPSLPTLIAFTIAILLLAVTPGPDMTLWISRSLREGRAAGFMTLVGTNIGITVHTMLVAFGVAALIVASPTAFMILK
TGGAAYLVWLAIQAIRKGSDFVMVKSTGEKAQASLKSALLNGIWVNLLNPKVIIFFMTFLPQFVSATDPHVTGKLIFLGI
WSIIVALPIGIGIVVTADILSAWLQRNRKVLRGLDYTIAGVFSLFAVKIFFTQTR

Specific function: Conducts the efflux of homoserine and homoserine lactone [H]

COG id: COG1280

COG function: function code E; Putative threonine efflux protein

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the rht family [H]

Homologues:

Organism=Escherichia coli, GI48994979, Length=195, Percent_Identity=29.2307692307692, Blast_Score=87, Evalue=9e-19,
Organism=Escherichia coli, GI1788099, Length=141, Percent_Identity=31.2056737588652, Blast_Score=67, Evalue=9e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004778
- InterPro:   IPR001123 [H]

Pfam domain/function: PF01810 LysE [H]

EC number: NA

Molecular weight: Translated: 23223; Mature: 23223

Theoretical pI: Translated: 11.08; Mature: 11.08

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDFVPSLPTLIAFTIAILLLAVTPGPDMTLWISRSLREGRAAGFMTLVGTNIGITVHTML
CCCCCCHHHHHHHHHHHHHHHCCCCCCEEEEEEHHHHCCCHHCCCEEECCCCCHHHHHHH
VAFGVAALIVASPTAFMILKTGGAAYLVWLAIQAIRKGSDFVMVKSTGEKAQASLKSALL
HHHHHHHHHHHCCCEEEEEECCCHHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHH
NGIWVNLLNPKVIIFFMTFLPQFVSATDPHVTGKLIFLGIWSIIVALPIGIGIVVTADIL
HHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SAWLQRNRKVLRGLDYTIAGVFSLFAVKIFFTQTR
HHHHHHHHHHHHCCCHHHHHHHHHHHHHHHEECCC
>Mature Secondary Structure
MDFVPSLPTLIAFTIAILLLAVTPGPDMTLWISRSLREGRAAGFMTLVGTNIGITVHTML
CCCCCCHHHHHHHHHHHHHHHCCCCCCEEEEEEHHHHCCCHHCCCEEECCCCCHHHHHHH
VAFGVAALIVASPTAFMILKTGGAAYLVWLAIQAIRKGSDFVMVKSTGEKAQASLKSALL
HHHHHHHHHHHCCCEEEEEECCCHHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHH
NGIWVNLLNPKVIIFFMTFLPQFVSATDPHVTGKLIFLGIWSIIVALPIGIGIVVTADIL
HHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SAWLQRNRKVLRGLDYTIAGVFSLFAVKIFFTQTR
HHHHHHHHHHHHCCCHHHHHHHHHHHHHHHEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; homoserine [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + homoserine lactone [Cytoplasm] = Proton [Cytoplasm] + homoserine lactone [Periplasm] Proton [Periplasm] + homoserine [Cytoplasm] = Proton [Cytoplasm] + homoserine [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]