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The map label for this gene is thiC

Identifier: 159185271

GI number: 159185271

Start: 2539907

End: 2541730

Strand: Reverse

Name: thiC

Synonym: Atu2569

Alternate gene names: 159185271

Gene position: 2541730-2539907 (Counterclockwise)

Preceding gene: 15889833

Following gene: 159185270

Centisome position: 89.45

GC content: 60.53

Gene sequence:

>1824_bases
ATGAATATCGTTGCCAAGACCATCCCGCTTGTCGTCACCACCGGGCCGCATGCCGCTTCCACGAAAATCCATAAACCGGG
AATTTTGCATCCGCATATTCGCGTGCCGATGCGCGAGATCGCGGTGCACCCAACGGCGGGCGAACCGCCGGTGACGGTGT
ATGATTCCTCCGGTCCCTATACCGATCCCAGCCATCCCGTCCTGATCGAAAACGGTCTTCCGCGCCTGCGTCATGACTGG
GTCGTGGCGCGCGGTGATGTCGAGGCTTATGAGGGACGGCACGTCAAGCCGGAGGATAACGGCTTTGCAACCGGCGAACG
CCTGACGCCGGAGTTTTCCGTGCGCCACCAGCCGCTGAAAGCGACGGCGGGCAAAGCGGTGACGCAGCTTGCCTATGCGC
GGGCCGGCATCATCACGCCGGAAATGGAATTCATCGCCATCCGCGAAAATCTCGGGCGCGAGGCCGCAAAGGAAAAGCCT
ACGCGGGACGGCGAGAGTTTCGGCGCGCATATCCCTGATTATGTCACGGCGGAATTCGTGCGTCAGGAAGTGGCATCGGG
TCGCGCCATCATTCCCGCCAATATCAACCATCCCGAACTTGAGCCGATGATCATCGGCCGCAATTTTCTGGTGAAGATCA
ACGCCAATATCGGCAATTCCGCCGTTACCTCCTCCATGGCGGAAGAGGTGGAGAAGATGGTCTGGGCCATCCGCTGGGGT
GCGGATACCGTCATGGACCTCTCCACCGGCCGTAACATCCACAATATCCGCGAATGGATCATTCGCAATTCACCGGTGCC
GATCGGCACGGTGCCGCTTTATCAGGCGCTGGAGAAGGTCAACGGCATTGCCGAAGACCTCAACTGGGAGGTGTTTCGCG
ATACGCTGATCGAGCAGGCGGAGCAGGGCGTCGATTATTTTACCATCCATGCCGGGGTGCGGCTGCATTACATCCCGCTC
ACCGTCAACCGCGTCACCGGCATCGTCTCGCGCGGCGGTTCGATCATGGCCAAGTGGTGTCTGCATCACCACAAGGAAAG
TTTCCTCTATGAGCACTTCGAGGAGATTTGCGACATCTGCCGCGCTTATGACGTCTCTTTCTCGCTGGGTGACGGCCTGC
GTCCCGGCTCGATTGCCGACGCCAATGATGCGGCGCAGTTTGCCGAGCTGGAGACGCTGGGCGAGCTGACGCAGATCGCC
TGGGCCAGGGATTGCCAGGTGATGATCGAAGGCCCCGGCCATGTGCCGATGCACAAGATCAAGGAAAACATGGACAAACA
GCTGAAGACCTGCGGCGAGGCGCCTTTCTATACGCTTGGGCCGCTCACGACAGATATCGCGCCCGGTTACGACCACATCA
CCTCAGGCATCGGGGCGGCGATGATCGGCTGGTTCGGCACGGCCATGCTCTGCTATGTCACGCCGAAGGAACATCTCGGT
CTGCCTGACCGCAACGACGTGAAGGTCGGTGTCATCACCTACAAGATCGCCGCCCACGCCGCCGATCTTGCCAAAGGCCA
TCCGGCCGCGCAGGTACGGGACGATGCGCTGTCGCGCGCGAGGTTCGAGTTCCGCTGGGAAGACCAGTTCAACCTGTCGC
TCGACCCGGATACCGCGCGTTCCTTCCATGATGAGACCCTGCCGAAAGAGGCTCACAAGGTTGCGCATTTCTGCTCCATG
TGCGGGCCGAAATTCTGCTCGATGCGGATTTCCCACGATATCCGTGCCGAGGCGCAGAAGGAGGGACTGGAAGCCATGGC
CGCCCGCTTCAAGGAGGGTGGTGAGCTTTATATGCCGCTGCCGACGCCCGCGAGCGCTGAATAA

Upstream 100 bases:

>100_bases
TGATGTCGGGCAGGCGCATGGCGCCGTGAGTCGGCACTTCGGTATTTTCCATCATTCCGGCTCGAACTGGGTCTCCACAG
TCAAACTTTGGAGCCTCAAG

Downstream 100 bases:

>100_bases
AATGCGTGTTCTCGTCAAGGGAACGGGGGTCGCCGGTTTGACGGCGGCCTTCGAACTGGTACGGAGTGGCGTCACCGTGG
AGGTTTCGGAGTGCCATGCC

Product: thiamine biosynthesis protein ThiC

Products: @AMIN06.txt*4-amino-2-methyl-5-hydroxymethylpyrimidine!; 4-amino-5-hydroxymethyl-2-methylpyrimidine-phosphate [C]

Alternate protein names: Hydroxymethylpyrimidine phosphate synthase; HMP-P synthase; HMP-phosphate synthase; HMPP synthase; Thiamine biosynthesis protein thiC

Number of amino acids: Translated: 607; Mature: 607

Protein sequence:

>607_residues
MNIVAKTIPLVVTTGPHAASTKIHKPGILHPHIRVPMREIAVHPTAGEPPVTVYDSSGPYTDPSHPVLIENGLPRLRHDW
VVARGDVEAYEGRHVKPEDNGFATGERLTPEFSVRHQPLKATAGKAVTQLAYARAGIITPEMEFIAIRENLGREAAKEKP
TRDGESFGAHIPDYVTAEFVRQEVASGRAIIPANINHPELEPMIIGRNFLVKINANIGNSAVTSSMAEEVEKMVWAIRWG
ADTVMDLSTGRNIHNIREWIIRNSPVPIGTVPLYQALEKVNGIAEDLNWEVFRDTLIEQAEQGVDYFTIHAGVRLHYIPL
TVNRVTGIVSRGGSIMAKWCLHHHKESFLYEHFEEICDICRAYDVSFSLGDGLRPGSIADANDAAQFAELETLGELTQIA
WARDCQVMIEGPGHVPMHKIKENMDKQLKTCGEAPFYTLGPLTTDIAPGYDHITSGIGAAMIGWFGTAMLCYVTPKEHLG
LPDRNDVKVGVITYKIAAHAADLAKGHPAAQVRDDALSRARFEFRWEDQFNLSLDPDTARSFHDETLPKEAHKVAHFCSM
CGPKFCSMRISHDIRAEAQKEGLEAMAARFKEGGELYMPLPTPASAE

Sequences:

>Translated_607_residues
MNIVAKTIPLVVTTGPHAASTKIHKPGILHPHIRVPMREIAVHPTAGEPPVTVYDSSGPYTDPSHPVLIENGLPRLRHDW
VVARGDVEAYEGRHVKPEDNGFATGERLTPEFSVRHQPLKATAGKAVTQLAYARAGIITPEMEFIAIRENLGREAAKEKP
TRDGESFGAHIPDYVTAEFVRQEVASGRAIIPANINHPELEPMIIGRNFLVKINANIGNSAVTSSMAEEVEKMVWAIRWG
ADTVMDLSTGRNIHNIREWIIRNSPVPIGTVPLYQALEKVNGIAEDLNWEVFRDTLIEQAEQGVDYFTIHAGVRLHYIPL
TVNRVTGIVSRGGSIMAKWCLHHHKESFLYEHFEEICDICRAYDVSFSLGDGLRPGSIADANDAAQFAELETLGELTQIA
WARDCQVMIEGPGHVPMHKIKENMDKQLKTCGEAPFYTLGPLTTDIAPGYDHITSGIGAAMIGWFGTAMLCYVTPKEHLG
LPDRNDVKVGVITYKIAAHAADLAKGHPAAQVRDDALSRARFEFRWEDQFNLSLDPDTARSFHDETLPKEAHKVAHFCSM
CGPKFCSMRISHDIRAEAQKEGLEAMAARFKEGGELYMPLPTPASAE
>Mature_607_residues
MNIVAKTIPLVVTTGPHAASTKIHKPGILHPHIRVPMREIAVHPTAGEPPVTVYDSSGPYTDPSHPVLIENGLPRLRHDW
VVARGDVEAYEGRHVKPEDNGFATGERLTPEFSVRHQPLKATAGKAVTQLAYARAGIITPEMEFIAIRENLGREAAKEKP
TRDGESFGAHIPDYVTAEFVRQEVASGRAIIPANINHPELEPMIIGRNFLVKINANIGNSAVTSSMAEEVEKMVWAIRWG
ADTVMDLSTGRNIHNIREWIIRNSPVPIGTVPLYQALEKVNGIAEDLNWEVFRDTLIEQAEQGVDYFTIHAGVRLHYIPL
TVNRVTGIVSRGGSIMAKWCLHHHKESFLYEHFEEICDICRAYDVSFSLGDGLRPGSIADANDAAQFAELETLGELTQIA
WARDCQVMIEGPGHVPMHKIKENMDKQLKTCGEAPFYTLGPLTTDIAPGYDHITSGIGAAMIGWFGTAMLCYVTPKEHLG
LPDRNDVKVGVITYKIAAHAADLAKGHPAAQVRDDALSRARFEFRWEDQFNLSLDPDTARSFHDETLPKEAHKVAHFCSM
CGPKFCSMRISHDIRAEAQKEGLEAMAARFKEGGELYMPLPTPASAE

Specific function: Catalyzes the synthesis of the hydroxymethylpyrimidine phosphate (HMP-P) moiety of thiamine from aminoimidazole ribotide (AIR) in a radical S-adenosyl-L-methionine (SAM)-dependent reaction

COG id: COG0422

COG function: function code H; Thiamine biosynthesis protein ThiC

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiC family

Homologues:

Organism=Escherichia coli, GI1790427, Length=598, Percent_Identity=68.8963210702341, Blast_Score=842, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): THIC_AGRT5 (Q8UCC9)

Other databases:

- EMBL:   AE007869
- PIR:   AH2891
- PIR:   D97667
- RefSeq:   NP_355508.2
- STRING:   Q8UCC9
- GeneID:   1134607
- GenomeReviews:   AE007869_GR
- KEGG:   atu:Atu2569
- eggNOG:   COG0422
- HOGENOM:   HBG285234
- OMA:   HLRFEAG
- PhylomeDB:   Q8UCC9
- ProtClustDB:   PRK09284
- BioCyc:   ATUM176299-1:ATU2569-MONOMER
- HAMAP:   MF_00089
- InterPro:   IPR002817
- TIGRFAMs:   TIGR00190

Pfam domain/function: PF01964 ThiC

EC number: NA

Molecular weight: Translated: 67075; Mature: 67075

Theoretical pI: Translated: 6.27; Mature: 6.27

Prosite motif: NA

Important sites: BINDING 216-216 BINDING 245-245 BINDING 274-274 BINDING 310-310 BINDING 410-410 BINDING 437-437

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNIVAKTIPLVVTTGPHAASTKIHKPGILHPHIRVPMREIAVHPTAGEPPVTVYDSSGPY
CCCCCCCCCEEEECCCCCCCCCCCCCCCCCCCCCCCHHHHEECCCCCCCCEEEECCCCCC
TDPSHPVLIENGLPRLRHDWVVARGDVEAYEGRHVKPEDNGFATGERLTPEFSVRHQPLK
CCCCCCEEECCCCCCCCCCEEEEECCHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHH
ATAGKAVTQLAYARAGIITPEMEFIAIRENLGREAAKEKPTRDGESFGAHIPDYVTAEFV
HHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCHHHHHCCCCCCCHHCCCCCCHHHHHHHH
RQEVASGRAIIPANINHPELEPMIIGRNFLVKINANIGNSAVTSSMAEEVEKMVWAIRWG
HHHHHCCCEEEECCCCCCCCCEEEECCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHCCC
ADTVMDLSTGRNIHNIREWIIRNSPVPIGTVPLYQALEKVNGIAEDLNWEVFRDTLIEQA
CCCEEECCCCCCHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHH
EQGVDYFTIHAGVRLHYIPLTVNRVTGIVSRGGSIMAKWCLHHHKESFLYEHFEEICDIC
HCCCCEEEEECCCEEEEEEEEHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
RAYDVSFSLGDGLRPGSIADANDAAQFAELETLGELTQIAWARDCQVMIEGPGHVPMHKI
HHHCCEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCHHHH
KENMDKQLKTCGEAPFYTLGPLTTDIAPGYDHITSGIGAAMIGWFGTAMLCYVTPKEHLG
HHHHHHHHHHHCCCCCEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHEEEEEECCHHHCC
LPDRNDVKVGVITYKIAAHAADLAKGHPAAQVRDDALSRARFEFRWEDQFNLSLDPDTAR
CCCCCCEEEEEEEEEHHHHHHHHHCCCCCHHHHHHHHHHCEEEEEECCCCCEEECCHHHH
SFHDETLPKEAHKVAHFCSMCGPKFCSMRISHDIRAEAQKEGLEAMAARFKEGGELYMPL
HHHHHCCHHHHHHHHHHHHHCCHHHHHEECCHHHHHHHHHHHHHHHHHHHCCCCEEEEEC
PTPASAE
CCCCCCC
>Mature Secondary Structure
MNIVAKTIPLVVTTGPHAASTKIHKPGILHPHIRVPMREIAVHPTAGEPPVTVYDSSGPY
CCCCCCCCCEEEECCCCCCCCCCCCCCCCCCCCCCCHHHHEECCCCCCCCEEEECCCCCC
TDPSHPVLIENGLPRLRHDWVVARGDVEAYEGRHVKPEDNGFATGERLTPEFSVRHQPLK
CCCCCCEEECCCCCCCCCCEEEEECCHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHH
ATAGKAVTQLAYARAGIITPEMEFIAIRENLGREAAKEKPTRDGESFGAHIPDYVTAEFV
HHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCHHHHHCCCCCCCHHCCCCCCHHHHHHHH
RQEVASGRAIIPANINHPELEPMIIGRNFLVKINANIGNSAVTSSMAEEVEKMVWAIRWG
HHHHHCCCEEEECCCCCCCCCEEEECCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHCCC
ADTVMDLSTGRNIHNIREWIIRNSPVPIGTVPLYQALEKVNGIAEDLNWEVFRDTLIEQA
CCCEEECCCCCCHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHH
EQGVDYFTIHAGVRLHYIPLTVNRVTGIVSRGGSIMAKWCLHHHKESFLYEHFEEICDIC
HCCCCEEEEECCCEEEEEEEEHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
RAYDVSFSLGDGLRPGSIADANDAAQFAELETLGELTQIAWARDCQVMIEGPGHVPMHKI
HHHCCEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCHHHH
KENMDKQLKTCGEAPFYTLGPLTTDIAPGYDHITSGIGAAMIGWFGTAMLCYVTPKEHLG
HHHHHHHHHHHCCCCCEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHEEEEEECCHHHCC
LPDRNDVKVGVITYKIAAHAADLAKGHPAAQVRDDALSRARFEFRWEDQFNLSLDPDTAR
CCCCCCEEEEEEEEEHHHHHHHHHCCCCCHHHHHHHHHHCEEEEEECCCCCEEECCHHHH
SFHDETLPKEAHKVAHFCSMCGPKFCSMRISHDIRAEAQKEGLEAMAARFKEGGELYMPL
HHHHHCCHHHHHHHHHHHHHCCHHHHHEECCHHHHHHHHHHHHHHHHHHHCCCCEEEEEC
PTPASAE
CCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ?; ?; 5'-phosphoribosyl-5-aminoimidazole [C]

Specific reaction: Synthesis of @AMIN06.txt*4-amino-2-methyl-5-hydroxymethylpyrimidine! 5'-phosphoribosyl-5-aminoimidazole = 4-amino-5-hydroxymethyl-2-methylpyrimidine-phosphate [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11743193; 11743194