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The map label for this gene is acrA [H]

Identifier: 159185263

GI number: 159185263

Start: 2521024

End: 2522226

Strand: Direct

Name: acrA [H]

Synonym: Atu2550

Alternate gene names: 159185263

Gene position: 2521024-2522226 (Clockwise)

Preceding gene: 15889808

Following gene: 15889810

Centisome position: 88.72

GC content: 60.27

Gene sequence:

>1203_bases
ATGAGCCGACGTATTTTACCCCTATTTGCTTCTCTGTGTGTAACCGCGGTTCTGGCGGGATGCAGCGATGGCGGCGAAAG
CAGCAAGTCCGCCGGCCAGGGTGCCGAGCGTCCGCCGTCTCCGGTCAGCGTCATCGTTATGAAGACCAGCGAATATCCTT
TGACCACCGTTCTTCCCGGCCGCGCCAGCGCGTTCCAGACGGCGGAAATCCGCCCGCGTGTGACCGGTATCATCCGCGAA
ATTCCCTTCAAGGAAGGCAGCGAAGTCAAGCAGGGCGACGTTCTCTACCAGATCGAGGACAACACCTATCTGGCGGAAGT
GGCACAGGCCAAGGCAAGCGTGGCGAAGGCGGAAGCCAGCGTTCCGAGCGCCCAGGCAAACCTTGCGCGCTACGAGCGGC
TGGTCAACAGCGGTGCGACGCAGATCGAATATGAAAACGCCAAGGTGACGCTGCTGCAGGCGCAAGCCGATGTGGCCCAG
ACCAAGGCGGCGCTGGAAACGGCGGAAATCAACCTCGATCTCACTAAAGTCCGCGCGCCTTTCGACGGCATTACCAGCGC
AACCGCCTTCAGCATCGGCAACGTGGTGACGGCAAACCAGACCACGGCCTTGACGACGCTGCGCCGCATCGATCCGATCT
ATATCGACCTGATGGAATCCAGCACCAATCTTCTGCGTCTTAAAAAGGCAATCTCCTCTGGTCAGCTCGGTGGCGACACG
AAGGAGACCGGCATCCATCTGACGCTGGAAGACGGCACTGAATATAAGCATGACGGCAAGATCGACATGTCCGACATGGC
GGTGAGCGAAACCACGGGCACCTTCTCCATCCGTGCCCTGTTCGAAAACCCTGATGATCTTCTGCTTCCCGGCACCTATG
TCCGTGCCACGCTGACCATCGGCAAGGAAAAGGGCTTCCGCATTCCGCAACGCGCCGCGAGCCGCAACGCCAGCGGCGAA
CTGACCGCGAAATTCGTGACGGCGGAAAACAAGGTGGAAACCCGCACCTTCCCGTCCAGCCAGCAATCGGGCAATGCCTG
GCTGGTGACGGAAAACGTCAAGGATGGCGACAAGCTCATCGTCGACGGCTTCCAGTGGATTGCCGAAGGCGCCACTGTCG
CACCGGTTGAAGTGACCATCGACGATCGCGGTATCGTCGTTCTGCCGCAGCAAGCTCCGCCTGCCGCCGCCCCCACGAAA
TAA

Upstream 100 bases:

>100_bases
CCCTTTCAAGGCCTTCGCCTGTCGTGTCTGTCTCCGGACGCGCATCCGCCGAGCGACATTGCGCTTATAGTTGAATGCGA
CCGATACGCAGGGAAGAACG

Downstream 100 bases:

>100_bases
GCATGAGCACGAAACCCTCGGTCGAAGCTCGTAAAAGGTAAGGCAGGACTGCCATGGCCCATTTCTTCATCCGGCGCCCC
GTTTTTGCATGGGTTATCGC

Product: RND multidrug efflux membrane permease

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 400; Mature: 399

Protein sequence:

>400_residues
MSRRILPLFASLCVTAVLAGCSDGGESSKSAGQGAERPPSPVSVIVMKTSEYPLTTVLPGRASAFQTAEIRPRVTGIIRE
IPFKEGSEVKQGDVLYQIEDNTYLAEVAQAKASVAKAEASVPSAQANLARYERLVNSGATQIEYENAKVTLLQAQADVAQ
TKAALETAEINLDLTKVRAPFDGITSATAFSIGNVVTANQTTALTTLRRIDPIYIDLMESSTNLLRLKKAISSGQLGGDT
KETGIHLTLEDGTEYKHDGKIDMSDMAVSETTGTFSIRALFENPDDLLLPGTYVRATLTIGKEKGFRIPQRAASRNASGE
LTAKFVTAENKVETRTFPSSQQSGNAWLVTENVKDGDKLIVDGFQWIAEGATVAPVEVTIDDRGIVVLPQQAPPAAAPTK

Sequences:

>Translated_400_residues
MSRRILPLFASLCVTAVLAGCSDGGESSKSAGQGAERPPSPVSVIVMKTSEYPLTTVLPGRASAFQTAEIRPRVTGIIRE
IPFKEGSEVKQGDVLYQIEDNTYLAEVAQAKASVAKAEASVPSAQANLARYERLVNSGATQIEYENAKVTLLQAQADVAQ
TKAALETAEINLDLTKVRAPFDGITSATAFSIGNVVTANQTTALTTLRRIDPIYIDLMESSTNLLRLKKAISSGQLGGDT
KETGIHLTLEDGTEYKHDGKIDMSDMAVSETTGTFSIRALFENPDDLLLPGTYVRATLTIGKEKGFRIPQRAASRNASGE
LTAKFVTAENKVETRTFPSSQQSGNAWLVTENVKDGDKLIVDGFQWIAEGATVAPVEVTIDDRGIVVLPQQAPPAAAPTK
>Mature_399_residues
SRRILPLFASLCVTAVLAGCSDGGESSKSAGQGAERPPSPVSVIVMKTSEYPLTTVLPGRASAFQTAEIRPRVTGIIREI
PFKEGSEVKQGDVLYQIEDNTYLAEVAQAKASVAKAEASVPSAQANLARYERLVNSGATQIEYENAKVTLLQAQADVAQT
KAALETAEINLDLTKVRAPFDGITSATAFSIGNVVTANQTTALTTLRRIDPIYIDLMESSTNLLRLKKAISSGQLGGDTK
ETGIHLTLEDGTEYKHDGKIDMSDMAVSETTGTFSIRALFENPDDLLLPGTYVRATLTIGKEKGFRIPQRAASRNASGEL
TAKFVTAENKVETRTFPSSQQSGNAWLVTENVKDGDKLIVDGFQWIAEGATVAPVEVTIDDRGIVVLPQQAPPAAAPTK

Specific function: AcrAB is a drug efflux protein with a broad substrate specificity [H]

COG id: COG0845

COG function: function code M; Membrane-fusion protein

Gene ontology:

Cell location: Cell inner membrane; Lipid-anchor [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the membrane fusion protein (MFP) (TC 8.A.1) family [H]

Homologues:

Organism=Escherichia coli, GI1786668, Length=401, Percent_Identity=40.1496259351621, Blast_Score=256, Evalue=2e-69,
Organism=Escherichia coli, GI1789665, Length=371, Percent_Identity=38.8140161725067, Blast_Score=242, Evalue=3e-65,
Organism=Escherichia coli, GI1789929, Length=372, Percent_Identity=37.3655913978495, Blast_Score=242, Evalue=3e-65,
Organism=Escherichia coli, GI87082036, Length=363, Percent_Identity=25.8953168044077, Blast_Score=124, Evalue=8e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006143 [H]

Pfam domain/function: PF00529 HlyD [H]

EC number: NA

Molecular weight: Translated: 42717; Mature: 42585

Theoretical pI: Translated: 4.96; Mature: 4.96

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRRILPLFASLCVTAVLAGCSDGGESSKSAGQGAERPPSPVSVIVMKTSEYPLTTVLPG
CCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCEEEEEEECCCCCEEEECCC
RASAFQTAEIRPRVTGIIREIPFKEGSEVKQGDVLYQIEDNTYLAEVAQAKASVAKAEAS
CCCCEEECCCCHHHHHHHHHCCCCCCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHCC
VPSAQANLARYERLVNSGATQIEYENAKVTLLQAQADVAQTKAALETAEINLDLTKVRAP
CCCHHHHHHHHHHHHHCCCCEEEECCCEEEEEECHHHHHHHHHHHHHEEEEEEEEEEECC
FDGITSATAFSIGNVVTANQTTALTTLRRIDPIYIDLMESSTNLLRLKKAISSGQLGGDT
CCCCCCHHHHCCCCEEECCCHHHHHHHHHCCCEEEEEECCCCHHHHHHHHHHCCCCCCCC
KETGIHLTLEDGTEYKHDGKIDMSDMAVSETTGTFSIRALFENPDDLLLPGTYVRATLTI
CCCCEEEEEECCCCCCCCCCEEHHHCEEECCCCCEEEEEEECCCCCEECCCCEEEEEEEE
GKEKGFRIPQRAASRNASGELTAKFVTAENKVETRTFPSSQQSGNAWLVTENVKDGDKLI
CCCCCCCCCHHHHCCCCCCCEEEEEEECCCCEEECCCCCCCCCCCEEEEECCCCCCCEEE
VDGFQWIAEGATVAPVEVTIDDRGIVVLPQQAPPAAAPTK
EECHHHHHCCCEEEEEEEEECCCCEEEECCCCCCCCCCCC
>Mature Secondary Structure 
SRRILPLFASLCVTAVLAGCSDGGESSKSAGQGAERPPSPVSVIVMKTSEYPLTTVLPG
CCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCEEEEEEECCCCCEEEECCC
RASAFQTAEIRPRVTGIIREIPFKEGSEVKQGDVLYQIEDNTYLAEVAQAKASVAKAEAS
CCCCEEECCCCHHHHHHHHHCCCCCCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHCC
VPSAQANLARYERLVNSGATQIEYENAKVTLLQAQADVAQTKAALETAEINLDLTKVRAP
CCCHHHHHHHHHHHHHCCCCEEEECCCEEEEEECHHHHHHHHHHHHHEEEEEEEEEEECC
FDGITSATAFSIGNVVTANQTTALTTLRRIDPIYIDLMESSTNLLRLKKAISSGQLGGDT
CCCCCCHHHHCCCCEEECCCHHHHHHHHHCCCEEEEEECCCCHHHHHHHHHHCCCCCCCC
KETGIHLTLEDGTEYKHDGKIDMSDMAVSETTGTFSIRALFENPDDLLLPGTYVRATLTI
CCCCEEEEEECCCCCCCCCCEEHHHCEEECCCCCEEEEEEECCCCCEECCCCEEEEEEEE
GKEKGFRIPQRAASRNASGELTAKFVTAENKVETRTFPSSQQSGNAWLVTENVKDGDKLI
CCCCCCCCCHHHHCCCCCCCEEEEEEECCCCEEECCCCCCCCCCCEEEEECCCCCCCEEE
VDGFQWIAEGATVAPVEVTIDDRGIVVLPQQAPPAAAPTK
EECHHHHHCCCEEEEEEEEECCCCEEEECCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]