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The map label for this gene is pssB [H]

Identifier: 159185211

GI number: 159185211

Start: 2400483

End: 2401325

Strand: Direct

Name: pssB [H]

Synonym: Atu2428

Alternate gene names: 159185211

Gene position: 2400483-2401325 (Clockwise)

Preceding gene: 15889690

Following gene: 159185443

Centisome position: 84.48

GC content: 62.87

Gene sequence:

>843_bases
ATGGCAAAACCCCGTTACGGGATGCCCCCGTTGCCGGATGGCTGTGGCAAAGAGATGATCGACATATTGACGAAAGCGGC
GCTTGACGCCGGACAGGCGATCATGGTGGTTCACCGCGCCGGCCCGCATGTGTCCTATAAGGACGACTGCTCGCCGGTAA
CCGAAGCCGATCAGCGCGCCGAGACGATTATTCTCGAGGCGCTGGCTGCCCATTTCCCTGAAATTCCCGTGGTTGCCGAA
GAGGCCGTGTCGAACGGCATCCTGCCGGAAACCGGGGCGGAGTTCTTTCTGGTCGATCCGCTTGACGGCACGAAGGAATT
CATTTCCGGCAAGGACGATTTCACCGTCAATATAGCGCTCATCCGCAATGGCGTGCCGGTGGCAGGCGTTGTCTACGCCC
CCTGTCGCGGCCAGGCATGGACGGGCAAGGACAACGCCGCCGAAAAGCTCGCAATATCAGGCGACGGCGCGATCCTGTCG
CGCCACCCCATCCGGGCCCGCCGACGCGGCGCTTCGCCTGTGGCGCTGATCAGCCGCTCGCATTGCACGGCAAAGACGGA
AGCCTTCGTGGCCGAACACGGGCTGAAAGACTGTATCTCGGTCGGTTCATCACTGAAATTCTGCATGCTGGCGGAGGGTG
CCGCCGATATCTACCCCCGCTTCAGCCGCACCATGATGTGGGACACCGCCGCCGGCGACGCCGTACTGCGTGCCGCAGGC
GGCCGGACACTCGATTGCGACGGGCAGCTTCTTGCCTATGAAGTTAGGGGCGACGGCGAGGATGCGCTGGCCAATCCGGA
TTTTATTGCCGAGGGAGCGATGGCGGTGGAGCAGGCCGGGTAA

Upstream 100 bases:

>100_bases
TTGGCAGGAAGTGCTGAAAAAATGGCGGTCGATGCCTATTTTAACATTGTCCAATGCCATTATTTGACGGGCAGCGGGCA
AAGGAGTAGCGAAGATCGAA

Downstream 100 bases:

>100_bases
GACTCGCGTGCAGTGTGCTGTGGTTCTTTGCGACGCGTGTCATTTGTAGAGCTTCAGAGTATGCCGCTTACCCCCCTCTG
CCCTGCCGGGCATCTCCCCC

Product: exopolysaccharide production protein PssB

Products: NA

Alternate protein names: 3'(2'),5-bisphosphonucleoside 3'(2')-phosphohydrolase; 3'-phosphoadenosine 5'-phosphate phosphatase; PAP phosphatase; DPNPase [H]

Number of amino acids: Translated: 280; Mature: 279

Protein sequence:

>280_residues
MAKPRYGMPPLPDGCGKEMIDILTKAALDAGQAIMVVHRAGPHVSYKDDCSPVTEADQRAETIILEALAAHFPEIPVVAE
EAVSNGILPETGAEFFLVDPLDGTKEFISGKDDFTVNIALIRNGVPVAGVVYAPCRGQAWTGKDNAAEKLAISGDGAILS
RHPIRARRRGASPVALISRSHCTAKTEAFVAEHGLKDCISVGSSLKFCMLAEGAADIYPRFSRTMMWDTAAGDAVLRAAG
GRTLDCDGQLLAYEVRGDGEDALANPDFIAEGAMAVEQAG

Sequences:

>Translated_280_residues
MAKPRYGMPPLPDGCGKEMIDILTKAALDAGQAIMVVHRAGPHVSYKDDCSPVTEADQRAETIILEALAAHFPEIPVVAE
EAVSNGILPETGAEFFLVDPLDGTKEFISGKDDFTVNIALIRNGVPVAGVVYAPCRGQAWTGKDNAAEKLAISGDGAILS
RHPIRARRRGASPVALISRSHCTAKTEAFVAEHGLKDCISVGSSLKFCMLAEGAADIYPRFSRTMMWDTAAGDAVLRAAG
GRTLDCDGQLLAYEVRGDGEDALANPDFIAEGAMAVEQAG
>Mature_279_residues
AKPRYGMPPLPDGCGKEMIDILTKAALDAGQAIMVVHRAGPHVSYKDDCSPVTEADQRAETIILEALAAHFPEIPVVAEE
AVSNGILPETGAEFFLVDPLDGTKEFISGKDDFTVNIALIRNGVPVAGVVYAPCRGQAWTGKDNAAEKLAISGDGAILSR
HPIRARRRGASPVALISRSHCTAKTEAFVAEHGLKDCISVGSSLKFCMLAEGAADIYPRFSRTMMWDTAAGDAVLRAAGG
RTLDCDGQLLAYEVRGDGEDALANPDFIAEGAMAVEQAG

Specific function: Converts 3'(2')-phosphoadenosine 5'-phosphate (PAP) to AMP. May also convert adenosine 3'-phosphate 5'-phosphosulfate (PAPS) to adenosine 5'-phosphosulfate (APS) [H]

COG id: COG1218

COG function: function code P; 3'-Phosphoadenosine 5'-phosphosulfate (PAPS) 3'-phosphatase

Gene ontology:

Cell location: Cytoplasm. Cell membrane; Peripheral membrane protein; Cytoplasmic side [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the inositol monophosphatase family [H]

Homologues:

Organism=Homo sapiens, GI7657236, Length=250, Percent_Identity=29.2, Blast_Score=75, Evalue=1e-13,
Organism=Homo sapiens, GI5031789, Length=245, Percent_Identity=26.1224489795918, Blast_Score=65, Evalue=9e-11,
Organism=Escherichia coli, GI1790659, Length=252, Percent_Identity=41.6666666666667, Blast_Score=158, Evalue=3e-40,
Organism=Escherichia coli, GI1788882, Length=246, Percent_Identity=27.6422764227642, Blast_Score=68, Evalue=6e-13,
Organism=Caenorhabditis elegans, GI71995897, Length=284, Percent_Identity=28.5211267605634, Blast_Score=79, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI71995905, Length=284, Percent_Identity=28.5211267605634, Blast_Score=79, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI193202570, Length=212, Percent_Identity=25.9433962264151, Blast_Score=65, Evalue=3e-11,
Organism=Caenorhabditis elegans, GI193202572, Length=212, Percent_Identity=28.7735849056604, Blast_Score=65, Evalue=5e-11,
Organism=Saccharomyces cerevisiae, GI6320493, Length=234, Percent_Identity=30.3418803418803, Blast_Score=80, Evalue=2e-16,
Organism=Drosophila melanogaster, GI21357329, Length=216, Percent_Identity=28.7037037037037, Blast_Score=79, Evalue=4e-15,
Organism=Drosophila melanogaster, GI24641722, Length=229, Percent_Identity=27.0742358078603, Blast_Score=73, Evalue=2e-13,
Organism=Drosophila melanogaster, GI24664926, Length=212, Percent_Identity=27.3584905660377, Blast_Score=72, Evalue=4e-13,
Organism=Drosophila melanogaster, GI21357957, Length=245, Percent_Identity=27.3469387755102, Blast_Score=70, Evalue=1e-12,
Organism=Drosophila melanogaster, GI24664922, Length=280, Percent_Identity=27.1428571428571, Blast_Score=70, Evalue=2e-12,
Organism=Drosophila melanogaster, GI24664918, Length=242, Percent_Identity=26.8595041322314, Blast_Score=69, Evalue=4e-12,
Organism=Drosophila melanogaster, GI21357303, Length=262, Percent_Identity=24.4274809160305, Blast_Score=68, Evalue=7e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006240
- InterPro:   IPR020583
- InterPro:   IPR000760
- InterPro:   IPR020550 [H]

Pfam domain/function: PF00459 Inositol_P [H]

EC number: =3.1.3.7 [H]

Molecular weight: Translated: 29553; Mature: 29422

Theoretical pI: Translated: 4.74; Mature: 4.74

Prosite motif: PS00629 IMP_1 ; PS00630 IMP_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKPRYGMPPLPDGCGKEMIDILTKAALDAGQAIMVVHRAGPHVSYKDDCSPVTEADQRA
CCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCCHHHHHHHH
ETIILEALAAHFPEIPVVAEEAVSNGILPETGAEFFLVDPLDGTKEFISGKDDFTVNIAL
HHHHHHHHHHHCCCCCCHHHHHHHCCCCCCCCCCEEEECCCCCHHHHHCCCCCEEEEEEE
IRNGVPVAGVVYAPCRGQAWTGKDNAAEKLAISGDGAILSRHPIRARRRGASPVALISRS
EECCCCEEEEEEECCCCCCCCCCCCCCCEEEECCCCCEEECCCHHHHHCCCCCEEEEECC
HCTAKTEAFVAEHGLKDCISVGSSLKFCMLAEGAADIYPRFSRTMMWDTAAGDAVLRAAG
CCCHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCHHHHHHHHHEEEEECCCCCHHHHHCC
GRTLDCDGQLLAYEVRGDGEDALANPDFIAEGAMAVEQAG
CCEECCCCCEEEEEECCCCCCCCCCCCHHHCCCHHHHCCC
>Mature Secondary Structure 
AKPRYGMPPLPDGCGKEMIDILTKAALDAGQAIMVVHRAGPHVSYKDDCSPVTEADQRA
CCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCCHHHHHHHH
ETIILEALAAHFPEIPVVAEEAVSNGILPETGAEFFLVDPLDGTKEFISGKDDFTVNIAL
HHHHHHHHHHHCCCCCCHHHHHHHCCCCCCCCCCEEEECCCCCHHHHHCCCCCEEEEEEE
IRNGVPVAGVVYAPCRGQAWTGKDNAAEKLAISGDGAILSRHPIRARRRGASPVALISRS
EECCCCEEEEEEECCCCCCCCCCCCCCCEEEECCCCCEEECCCHHHHHCCCCCEEEEECC
HCTAKTEAFVAEHGLKDCISVGSSLKFCMLAEGAADIYPRFSRTMMWDTAAGDAVLRAAG
CCCHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCHHHHHHHHHEEEEECCCCCHHHHHCC
GRTLDCDGQLLAYEVRGDGEDALANPDFIAEGAMAVEQAG
CCEECCCCCEEEEEECCCCCCCCCCCCHHHCCCHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 10993077 [H]