Click here to switch to the map view.

The map label for this gene is pth

Identifier: 159185111

GI number: 159185111

Start: 2202862

End: 2203578

Strand: Direct

Name: pth

Synonym: Atu2230

Alternate gene names: 159185111

Gene position: 2202862-2203578 (Clockwise)

Preceding gene: 159185110

Following gene: 15889507

Centisome position: 77.52

GC content: 60.81

Gene sequence:

>717_bases
ATGAAGATCATCGCAGGACTTGGTAATCCCGGCGCACAATATGCGGGAAACCGTCACAATATCGGCTTCATGGCCGTGGA
TGCGCTGCAGCGCCTGCCCTCTTTTGCGCCCTGGTCGAAGAAGTTCAAGGCGGAGATTTCCGAAGGTGAGATCGGCGGCG
AGAAGGTTCTGCTGATGAAACCGCTCACCTATATGAACCTGTCAGGCGAATCCGTCGGCGAGGCCATGCGTTTCTTCAAA
CTGACGCCGGCCGACATCATCGCCATCCATGACGAACTCGATTTGCTGGCCGGCCGCACACGCATCAAGATCGGCGGCGG
CCACGGCGGTCATAACGGCCTTAAATCGCTCGACGCCCATTGCGGCAAGGAATACCGCCGTCTGCGCCTCGGCATCGGCC
ATCCCGGTGACAAGGAGCGGGTGCACGGCCATGTGCTCGGCGATTTCGCCAAGTCGGACCGCGTCTGGCTCGATCCGCTG
CTGGACGCCATTGCCGACAACGCCGCCATGCTGGTGAAGGGTGACGATTCGCAGCTGATGAACAAGCTGGCGCTGGCGAC
GGGCAGCAAACCGGAAGCGGAAAAACCGGTAAAGGTCGCCAAGCCGGCCGCGCAGTCCCACATCCACCAGGCGCGCAACA
GCGCCCAGCCGAAAAAGCTGCCGGAAACCGGACCAATGGCCGAGATGCTGAAGCGAATGTTCGGCAAGAAGGACTAA

Upstream 100 bases:

>100_bases
GCAATCGGCCGATCCGCCCGCGAACCCATAAAAAGATTGACATCAGGCACTTTTCGGGGTGGTGACAACAGCATCATCAG
GCAAAACGCGGACATGCCCC

Downstream 100 bases:

>100_bases
AACTCGCATGTCTTCCGACGCTATTTCCCTGCGGGCGGCCGGCCCCGGCGATCTACCCGGTTTGCTTGAACTCTACCAGG
TACTAAACCCATCAGACCCG

Product: peptidyl-tRNA hydrolase

Products: NA

Alternate protein names: PTH

Number of amino acids: Translated: 238; Mature: 238

Protein sequence:

>238_residues
MKIIAGLGNPGAQYAGNRHNIGFMAVDALQRLPSFAPWSKKFKAEISEGEIGGEKVLLMKPLTYMNLSGESVGEAMRFFK
LTPADIIAIHDELDLLAGRTRIKIGGGHGGHNGLKSLDAHCGKEYRRLRLGIGHPGDKERVHGHVLGDFAKSDRVWLDPL
LDAIADNAAMLVKGDDSQLMNKLALATGSKPEAEKPVKVAKPAAQSHIHQARNSAQPKKLPETGPMAEMLKRMFGKKD

Sequences:

>Translated_238_residues
MKIIAGLGNPGAQYAGNRHNIGFMAVDALQRLPSFAPWSKKFKAEISEGEIGGEKVLLMKPLTYMNLSGESVGEAMRFFK
LTPADIIAIHDELDLLAGRTRIKIGGGHGGHNGLKSLDAHCGKEYRRLRLGIGHPGDKERVHGHVLGDFAKSDRVWLDPL
LDAIADNAAMLVKGDDSQLMNKLALATGSKPEAEKPVKVAKPAAQSHIHQARNSAQPKKLPETGPMAEMLKRMFGKKD
>Mature_238_residues
MKIIAGLGNPGAQYAGNRHNIGFMAVDALQRLPSFAPWSKKFKAEISEGEIGGEKVLLMKPLTYMNLSGESVGEAMRFFK
LTPADIIAIHDELDLLAGRTRIKIGGGHGGHNGLKSLDAHCGKEYRRLRLGIGHPGDKERVHGHVLGDFAKSDRVWLDPL
LDAIADNAAMLVKGDDSQLMNKLALATGSKPEAEKPVKVAKPAAQSHIHQARNSAQPKKLPETGPMAEMLKRMFGKKD

Specific function: The natural substrate for this enzyme may be peptidyl- tRNAs which drop off the ribosome during protein synthesis

COG id: COG0193

COG function: function code J; Peptidyl-tRNA hydrolase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PTH family

Homologues:

Organism=Homo sapiens, GI50897284, Length=168, Percent_Identity=36.9047619047619, Blast_Score=103, Evalue=1e-22,
Organism=Escherichia coli, GI1787455, Length=186, Percent_Identity=44.0860215053763, Blast_Score=123, Evalue=1e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PTH_AGRT5 (Q8UD97)

Other databases:

- EMBL:   AE007869
- PIR:   AE2850
- PIR:   C97627
- RefSeq:   NP_355187.2
- ProteinModelPortal:   Q8UD97
- SMR:   Q8UD97
- STRING:   Q8UD97
- GeneID:   1134268
- GenomeReviews:   AE007869_GR
- KEGG:   atu:Atu2230
- eggNOG:   COG0193
- HOGENOM:   HBG610927
- OMA:   HIRERSQ
- PhylomeDB:   Q8UD97
- ProtClustDB:   PRK05426
- BioCyc:   ATUM176299-1:ATU2230-MONOMER
- GO:   GO:0005737
- GO:   GO:0006412
- HAMAP:   MF_00083
- InterPro:   IPR001328
- InterPro:   IPR018171
- Gene3D:   G3DSA:3.40.50.1470
- PANTHER:   PTHR17224
- TIGRFAMs:   TIGR00447

Pfam domain/function: PF01195 Pept_tRNA_hydro; SSF53178 Pept_tRNA_hydro

EC number: =3.1.1.29

Molecular weight: Translated: 25797; Mature: 25797

Theoretical pI: Translated: 10.17; Mature: 10.17

Prosite motif: PS01195 PEPT_TRNA_HYDROL_1; PS01196 PEPT_TRNA_HYDROL_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIIAGLGNPGAQYAGNRHNIGFMAVDALQRLPSFAPWSKKFKAEISEGEIGGEKVLLMK
CEEEECCCCCCHHCCCCCCCCHHHHHHHHHHCCCCCCCCHHHHHHCCCCCCCCCEEEEEC
PLTYMNLSGESVGEAMRFFKLTPADIIAIHDELDLLAGRTRIKIGGGHGGHNGLKSLDAH
CCHHCCCCCHHHHHHHHHHCCCCHHEEEECCHHHHHCCCEEEEECCCCCCCHHHHHHHHH
CGKEYRRLRLGIGHPGDKERVHGHVLGDFAKSDRVWLDPLLDAIADNAAMLVKGDDSQLM
HHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCEEEEECCHHHHH
NKLALATGSKPEAEKPVKVAKPAAQSHIHQARNSAQPKKLPETGPMAEMLKRMFGKKD
HHHHHHCCCCCCCCCCHHHHCHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHCCCC
>Mature Secondary Structure
MKIIAGLGNPGAQYAGNRHNIGFMAVDALQRLPSFAPWSKKFKAEISEGEIGGEKVLLMK
CEEEECCCCCCHHCCCCCCCCHHHHHHHHHHCCCCCCCCHHHHHHCCCCCCCCCEEEEEC
PLTYMNLSGESVGEAMRFFKLTPADIIAIHDELDLLAGRTRIKIGGGHGGHNGLKSLDAH
CCHHCCCCCHHHHHHHHHHCCCCHHEEEECCHHHHHCCCEEEEECCCCCCCHHHHHHHHH
CGKEYRRLRLGIGHPGDKERVHGHVLGDFAKSDRVWLDPLLDAIADNAAMLVKGDDSQLM
HHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCEEEEECCHHHHH
NKLALATGSKPEAEKPVKVAKPAAQSHIHQARNSAQPKKLPETGPMAEMLKRMFGKKD
HHHHHHCCCCCCCCCCHHHHCHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11743193; 11743194