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The map label for this gene is thyA

Identifier: 159185026

GI number: 159185026

Start: 2005855

End: 2006649

Strand: Reverse

Name: thyA

Synonym: Atu2047

Alternate gene names: 159185026

Gene position: 2006649-2005855 (Counterclockwise)

Preceding gene: 159185438

Following gene: 15889333

Centisome position: 70.62

GC content: 56.6

Gene sequence:

>795_bases
ATGAAACAATATCTCGATCTTCTCCGGCATGTGATGGAAACCGGCTCCGACCGCGGAGACCGCACCGGCACCGGCACGCG
TTCGGTATTCGGTTACCAGATGCGTTTCGACCTTTCCGAAGGTTTTCCGGTTCTCACCACAAAGAAACTGCATCTGCGCT
CCATCATCCATGAGCTGCTGTGGTTCCTAAACGGCGATACCAATATTGCCTATCTCAAGGAAAATGGTGTTTCCATCTGG
GATGAATGGGCCGATGAGAACGGCGATCTCGGTCCGGTCTATGGCGCGCAATGGCGGTCCTGGCCAGCGCCTGATGGCCG
CCATATCGACCAGATCGCGCTTCTGATCGAGGCGCTTAAAACCAATCCCAATTCCCGCCGCCACATCGTTTCGGCCTGGA
ACCCGGCGCTGGTGGACGAAATGGCGCTGCCGCCCTGCCATTGCCTGTTCCAGTTCTATGTGTCCGATGGAAAGCTGTCC
TGCCAGCTTTACCAGCGCTCGGCCGATATTTTCCTCGGCGTGCCCTTCAATATCGCATCCTACGCGCTGCTGACGCTGAT
GGTGGCGCAGGTCGTGGGCCTCAAGCCCGGCGATTTCGTTCATACCCTTGGTGATGCGCATATCTACGCCAACCATTTCG
AACAGGCGCAGCTGCAGATGACGCGTACGCCGAAGGCCTTGCCGACCATGCGCCTCAACCCTGATGTGAAGAACCTTTTC
GGCTTTAAGTTCGAGGACTTTACGCTGGAGAATTACGAGGCCGATTCAAGCATCAAGGCACCGATCGCGGTATGA

Upstream 100 bases:

>100_bases
CGCTTTGATCTGAGCCGGGGATAAACCCGCATTAGGTGATTGGTTCTGCCGCCCTGTGCTATTATATTCCCTTGATATCA
GGGAATCGGCGGCAGAACGC

Downstream 100 bases:

>100_bases
GTGAGCCGCGCGTCACCATCATCGTTGCGGTCTCCGAGAACGGCGTCATCGGCCGCGATCTCGACATGCCATGGAAGCTG
TCGACCGATCTGAAGCGCTT

Product: thymidylate synthase

Products: NA

Alternate protein names: TS; TSase

Number of amino acids: Translated: 264; Mature: 264

Protein sequence:

>264_residues
MKQYLDLLRHVMETGSDRGDRTGTGTRSVFGYQMRFDLSEGFPVLTTKKLHLRSIIHELLWFLNGDTNIAYLKENGVSIW
DEWADENGDLGPVYGAQWRSWPAPDGRHIDQIALLIEALKTNPNSRRHIVSAWNPALVDEMALPPCHCLFQFYVSDGKLS
CQLYQRSADIFLGVPFNIASYALLTLMVAQVVGLKPGDFVHTLGDAHIYANHFEQAQLQMTRTPKALPTMRLNPDVKNLF
GFKFEDFTLENYEADSSIKAPIAV

Sequences:

>Translated_264_residues
MKQYLDLLRHVMETGSDRGDRTGTGTRSVFGYQMRFDLSEGFPVLTTKKLHLRSIIHELLWFLNGDTNIAYLKENGVSIW
DEWADENGDLGPVYGAQWRSWPAPDGRHIDQIALLIEALKTNPNSRRHIVSAWNPALVDEMALPPCHCLFQFYVSDGKLS
CQLYQRSADIFLGVPFNIASYALLTLMVAQVVGLKPGDFVHTLGDAHIYANHFEQAQLQMTRTPKALPTMRLNPDVKNLF
GFKFEDFTLENYEADSSIKAPIAV
>Mature_264_residues
MKQYLDLLRHVMETGSDRGDRTGTGTRSVFGYQMRFDLSEGFPVLTTKKLHLRSIIHELLWFLNGDTNIAYLKENGVSIW
DEWADENGDLGPVYGAQWRSWPAPDGRHIDQIALLIEALKTNPNSRRHIVSAWNPALVDEMALPPCHCLFQFYVSDGKLS
CQLYQRSADIFLGVPFNIASYALLTLMVAQVVGLKPGDFVHTLGDAHIYANHFEQAQLQMTRTPKALPTMRLNPDVKNLF
GFKFEDFTLENYEADSSIKAPIAV

Specific function: Provides the sole de novo source of dTMP for DNA biosynthesis

COG id: COG0207

COG function: function code F; Thymidylate synthase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thymidylate synthase family. Bacterial- type thyA subfamily

Homologues:

Organism=Homo sapiens, GI4507751, Length=282, Percent_Identity=50, Blast_Score=286, Evalue=1e-77,
Organism=Escherichia coli, GI1789191, Length=264, Percent_Identity=63.6363636363636, Blast_Score=375, Evalue=1e-105,
Organism=Caenorhabditis elegans, GI71993377, Length=283, Percent_Identity=45.9363957597173, Blast_Score=265, Evalue=2e-71,
Organism=Saccharomyces cerevisiae, GI6324648, Length=292, Percent_Identity=41.7808219178082, Blast_Score=212, Evalue=6e-56,
Organism=Drosophila melanogaster, GI17137556, Length=283, Percent_Identity=51.5901060070671, Blast_Score=291, Evalue=3e-79,

Paralogues:

None

Copy number: 391 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): TYSY_AGRT5 (Q8UDS3)

Other databases:

- EMBL:   AE007869
- PIR:   AI2827
- PIR:   G97605
- RefSeq:   NP_355015.2
- ProteinModelPortal:   Q8UDS3
- SMR:   Q8UDS3
- STRING:   Q8UDS3
- GeneID:   1134085
- GenomeReviews:   AE007869_GR
- KEGG:   atu:Atu2047
- eggNOG:   COG0207
- HOGENOM:   HBG588098
- OMA:   RMALAPC
- PhylomeDB:   Q8UDS3
- ProtClustDB:   PRK01827
- BioCyc:   ATUM176299-1:ATU2047-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00008
- InterPro:   IPR000398
- InterPro:   IPR020940
- Gene3D:   G3DSA:3.30.572.10
- PANTHER:   PTHR11549:SF2
- PRINTS:   PR00108
- TIGRFAMs:   TIGR03284

Pfam domain/function: PF00303 Thymidylat_synt; SSF55831 Thymidylat_synth_C

EC number: =2.1.1.45

Molecular weight: Translated: 29875; Mature: 29875

Theoretical pI: Translated: 6.23; Mature: 6.23

Prosite motif: PS00091 THYMIDYLATE_SYNTHASE

Important sites: ACT_SITE 161-161

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKQYLDLLRHVMETGSDRGDRTGTGTRSVFGYQMRFDLSEGFPVLTTKKLHLRSIIHELL
CHHHHHHHHHHHHCCCCCCCCCCCCCHHEEEEEEEEECCCCCCEEECCHHHHHHHHHHHH
WFLNGDTNIAYLKENGVSIWDEWADENGDLGPVYGAQWRSWPAPDGRHIDQIALLIEALK
HHHCCCCEEEEEECCCCCHHHHHCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHH
TNPNSRRHIVSAWNPALVDEMALPPCHCLFQFYVSDGKLSCQLYQRSADIFLGVPFNIAS
CCCCCCEEEEECCCHHHHHHHCCCHHHHHHHHHHCCCCEEEEEEECCCCEEEECCHHHHH
YALLTLMVAQVVGLKPGDFVHTLGDAHIYANHFEQAQLQMTRTPKALPTMRLNPDVKNLF
HHHHHHHHHHHHCCCCCCHHHHCCCCCEEHHHHHHHHHHHHCCCCCCCCEECCCCHHHHH
GFKFEDFTLENYEADSSIKAPIAV
CCCEECEEECCCCCCCCCCCCCCC
>Mature Secondary Structure
MKQYLDLLRHVMETGSDRGDRTGTGTRSVFGYQMRFDLSEGFPVLTTKKLHLRSIIHELL
CHHHHHHHHHHHHCCCCCCCCCCCCCHHEEEEEEEEECCCCCCEEECCHHHHHHHHHHHH
WFLNGDTNIAYLKENGVSIWDEWADENGDLGPVYGAQWRSWPAPDGRHIDQIALLIEALK
HHHCCCCEEEEEECCCCCHHHHHCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHH
TNPNSRRHIVSAWNPALVDEMALPPCHCLFQFYVSDGKLSCQLYQRSADIFLGVPFNIAS
CCCCCCEEEEECCCHHHHHHHCCCHHHHHHHHHHCCCCEEEEEEECCCCEEEECCHHHHH
YALLTLMVAQVVGLKPGDFVHTLGDAHIYANHFEQAQLQMTRTPKALPTMRLNPDVKNLF
HHHHHHHHHHHHCCCCCCHHHHCCCCCEEHHHHHHHHHHHHCCCCCCCCEECCCCHHHHH
GFKFEDFTLENYEADSSIKAPIAV
CCCEECEEECCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11743193; 11743194