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The map label for this gene is folA [H]

Identifier: 15889333

GI number: 15889333

Start: 2005331

End: 2005858

Strand: Reverse

Name: folA [H]

Synonym: Atu2046

Alternate gene names: 15889333

Gene position: 2005858-2005331 (Counterclockwise)

Preceding gene: 159185026

Following gene: 159185025

Centisome position: 70.59

GC content: 57.58

Gene sequence:

>528_bases
ATGAGTGAGCCGCGCGTCACCATCATCGTTGCGGTCTCCGAGAACGGCGTCATCGGCCGCGATCTCGACATGCCATGGAA
GCTGTCGACCGATCTGAAGCGCTTCAAGGCGATGACGATGGGCAAGCCGCTCATCATGGGCCGCAAGACGTTTCTGTCTG
TCGGCGAACGTCCCCTCCCGGGCCGTCCGCATATCATCGTCAGCCGAAATGCCGATTACCGGCCTGAAGGCGTCGACGTC
GTTTCGTCGCTGGACGAGGCCGTGAAGCTTGCAAAAACGAAGGCTGTGGAACTGGGCGTGGACGAGGTTTTTGTCGCCGG
CGGTGGCGAGATTTACCGTCAGGCCATGCCCTTTGCCGATCAACTTTCCGTCACCCATGTGGCGGTAAAACTCGATGGCG
ACACTTTTTTTCCGGAAATTGATCCCGCCGTTTTTGAGAAAACCGAAGAAAAGGCCGCGTCCGCAGGGGAAAAGGACAAT
TATCCGGTTCTGTTTACCACTTATGTGCGACGAGCCGCGGCAAAGTGA

Upstream 100 bases:

>100_bases
GCCTCAACCCTGATGTGAAGAACCTTTTCGGCTTTAAGTTCGAGGACTTTACGCTGGAGAATTACGAGGCCGATTCAAGC
ATCAAGGCACCGATCGCGGT

Downstream 100 bases:

>100_bases
ACTATTTACCTCGATTGGGTATTTAGTTACCACCTCTGCGTTGAAACAGGCGCTTCTCCTTCCTATAACGGGTCGATATT
CCCGCCGCACGCGAGCAATC

Product: dihydrofolate reductase

Products: NA

Alternate protein names: Dihydrofolate reductase type III [H]

Number of amino acids: Translated: 175; Mature: 174

Protein sequence:

>175_residues
MSEPRVTIIVAVSENGVIGRDLDMPWKLSTDLKRFKAMTMGKPLIMGRKTFLSVGERPLPGRPHIIVSRNADYRPEGVDV
VSSLDEAVKLAKTKAVELGVDEVFVAGGGEIYRQAMPFADQLSVTHVAVKLDGDTFFPEIDPAVFEKTEEKAASAGEKDN
YPVLFTTYVRRAAAK

Sequences:

>Translated_175_residues
MSEPRVTIIVAVSENGVIGRDLDMPWKLSTDLKRFKAMTMGKPLIMGRKTFLSVGERPLPGRPHIIVSRNADYRPEGVDV
VSSLDEAVKLAKTKAVELGVDEVFVAGGGEIYRQAMPFADQLSVTHVAVKLDGDTFFPEIDPAVFEKTEEKAASAGEKDN
YPVLFTTYVRRAAAK
>Mature_174_residues
SEPRVTIIVAVSENGVIGRDLDMPWKLSTDLKRFKAMTMGKPLIMGRKTFLSVGERPLPGRPHIIVSRNADYRPEGVDVV
SSLDEAVKLAKTKAVELGVDEVFVAGGGEIYRQAMPFADQLSVTHVAVKLDGDTFFPEIDPAVFEKTEEKAASAGEKDNY
PVLFTTYVRRAAAK

Specific function: Key enzyme in folate metabolism. Catalyzes an essential reaction for de novo glycine and purine synthesis, and for DNA precursor synthesis [H]

COG id: COG0262

COG function: function code H; Dihydrofolate reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 DHFR (dihydrofolate reductase) domain [H]

Homologues:

Organism=Homo sapiens, GI4503323, Length=149, Percent_Identity=40.2684563758389, Blast_Score=97, Evalue=9e-21,
Organism=Homo sapiens, GI28827793, Length=149, Percent_Identity=36.9127516778523, Blast_Score=83, Evalue=1e-16,
Organism=Escherichia coli, GI1786233, Length=168, Percent_Identity=38.0952380952381, Blast_Score=110, Evalue=6e-26,
Organism=Caenorhabditis elegans, GI17505899, Length=163, Percent_Identity=27.6073619631902, Blast_Score=70, Evalue=8e-13,
Organism=Drosophila melanogaster, GI116008026, Length=150, Percent_Identity=36, Blast_Score=86, Evalue=1e-17,
Organism=Drosophila melanogaster, GI24647458, Length=150, Percent_Identity=36, Blast_Score=86, Evalue=1e-17,

Paralogues:

None

Copy number: 640 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 10,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012259
- InterPro:   IPR017925
- InterPro:   IPR001796 [H]

Pfam domain/function: PF00186 DHFR_1 [H]

EC number: =1.5.1.3 [H]

Molecular weight: Translated: 19197; Mature: 19066

Theoretical pI: Translated: 6.55; Mature: 6.55

Prosite motif: PS00075 DHFR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEPRVTIIVAVSENGVIGRDLDMPWKLSTDLKRFKAMTMGKPLIMGRKTFLSVGERPLP
CCCCCEEEEEEECCCCCEECCCCCCCCCCHHHHHHHHHHCCCCEEECCHHHHHCCCCCCC
GRPHIIVSRNADYRPEGVDVVSSLDEAVKLAKTKAVELGVDEVFVAGGGEIYRQAMPFAD
CCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHEEECCCEEEEECCHHHHHHHCCCHH
QLSVTHVAVKLDGDTFFPEIDPAVFEKTEEKAASAGEKDNYPVLFTTYVRRAAAK
HCEEEEEEEEECCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCEEHHHHHHHHHCC
>Mature Secondary Structure 
SEPRVTIIVAVSENGVIGRDLDMPWKLSTDLKRFKAMTMGKPLIMGRKTFLSVGERPLP
CCCCEEEEEEECCCCCEECCCCCCCCCCHHHHHHHHHHCCCCEEECCHHHHHCCCCCCC
GRPHIIVSRNADYRPEGVDVVSSLDEAVKLAKTKAVELGVDEVFVAGGGEIYRQAMPFAD
CCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHEEECCCEEEEECCHHHHHHHCCCHH
QLSVTHVAVKLDGDTFFPEIDPAVFEKTEEKAASAGEKDNYPVLFTTYVRRAAAK
HCEEEEEEEEECCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCEEHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2840679; 6371010 [H]