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The map label for this gene is rhtB [H]

Identifier: 159184952

GI number: 159184952

Start: 1844353

End: 1844991

Strand: Reverse

Name: rhtB [H]

Synonym: Atu1864

Alternate gene names: 159184952

Gene position: 1844991-1844353 (Counterclockwise)

Preceding gene: 159184953

Following gene: 159184951

Centisome position: 64.93

GC content: 61.82

Gene sequence:

>639_bases
ATGCCGCTGGAGAACTGGCTGGCCTTCGTGGCCGCATCCGCCATCATGCTCGCCATTCCGGGACCGACGATCCTGCTGGT
GATTTCTTATGCGCTTGGCCATGGTCGCAAGGCGAGCACGGCGACGGTGACGGGCGTGGCGCTCGGTGATTTCACCGCCA
TGACGGCCTCGATGCTGGGGCTTGGCGCGCTGCTTGCCACGTCGGCTGCACTTTTCACCGGCCTGAAATGGATCGGTGCT
GCTTACCTCATCTATCTCGGCATCAAGCTGTGGCGTTCGCCGGTTGGTGGCGAAGGCGCGGATGGCCAAGGCGTGACGGG
CCGTGAAAGGCCGCTCAAAATCTTCCTGCATGCCTATATCGTCACGGCGCTGAACCCCAAGAGCATCGTGTTCTTCGTGG
CCTTCCTGCCGCAGTTTCTGGTGCCGACATTGCCGTTTTGGCCGCAGGTGCTGATTTTCGAAGCGACGTTCCTGGTGCTT
GCCACCGTGAATGCGGCGCTTTATGGACTTCTGGCAAGTGCCGCCCGTAACACGATCCGCAAGCCGAAGGTTCAGCGCAT
CGTCAACCGCACGGGCGGCGGTCTCCTGATTGGCGCCGGTCTCATTGCCTTTGGCTGGAAGAGGGCGGTTTCGGCGTGA

Upstream 100 bases:

>100_bases
CGTCCGCTGGATGACAGGGCGGCTTGATCTTCGAAGATTGAGCTTGATACCTCCTGATTTTGGTGGCTTGCTCGACACAT
TAGACAAGGGAGACTCAATC

Downstream 100 bases:

>100_bases
AACGCCCGTCGATCGCCTGAAATTCGGACATAACAAAATGGCATTGCCGCATATTTTTCTTGCCCTGATCACCGTGTTTT
TGTGGGGCTTCAACTTCGTT

Product: putative homoserine/homoserine lactone efflux protein

Products: homoserine lactone [Periplasm]; Proton [Cytoplasm]; homoserine [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 212; Mature: 211

Protein sequence:

>212_residues
MPLENWLAFVAASAIMLAIPGPTILLVISYALGHGRKASTATVTGVALGDFTAMTASMLGLGALLATSAALFTGLKWIGA
AYLIYLGIKLWRSPVGGEGADGQGVTGRERPLKIFLHAYIVTALNPKSIVFFVAFLPQFLVPTLPFWPQVLIFEATFLVL
ATVNAALYGLLASAARNTIRKPKVQRIVNRTGGGLLIGAGLIAFGWKRAVSA

Sequences:

>Translated_212_residues
MPLENWLAFVAASAIMLAIPGPTILLVISYALGHGRKASTATVTGVALGDFTAMTASMLGLGALLATSAALFTGLKWIGA
AYLIYLGIKLWRSPVGGEGADGQGVTGRERPLKIFLHAYIVTALNPKSIVFFVAFLPQFLVPTLPFWPQVLIFEATFLVL
ATVNAALYGLLASAARNTIRKPKVQRIVNRTGGGLLIGAGLIAFGWKRAVSA
>Mature_211_residues
PLENWLAFVAASAIMLAIPGPTILLVISYALGHGRKASTATVTGVALGDFTAMTASMLGLGALLATSAALFTGLKWIGAA
YLIYLGIKLWRSPVGGEGADGQGVTGRERPLKIFLHAYIVTALNPKSIVFFVAFLPQFLVPTLPFWPQVLIFEATFLVLA
TVNAALYGLLASAARNTIRKPKVQRIVNRTGGGLLIGAGLIAFGWKRAVSA

Specific function: Conducts the efflux of homoserine and homoserine lactone [H]

COG id: COG1280

COG function: function code E; Putative threonine efflux protein

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the rht family [H]

Homologues:

Organism=Escherichia coli, GI48994979, Length=200, Percent_Identity=32.5, Blast_Score=101, Evalue=4e-23,
Organism=Escherichia coli, GI1788099, Length=143, Percent_Identity=32.1678321678322, Blast_Score=74, Evalue=8e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004778
- InterPro:   IPR001123 [H]

Pfam domain/function: PF01810 LysE [H]

EC number: NA

Molecular weight: Translated: 22338; Mature: 22207

Theoretical pI: Translated: 11.06; Mature: 11.06

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPLENWLAFVAASAIMLAIPGPTILLVISYALGHGRKASTATVTGVALGDFTAMTASMLG
CCHHHHHHHHHHHHHHEECCCCHHHHHHHHHHCCCCCCCCCEEEEEHHHHHHHHHHHHHH
LGALLATSAALFTGLKWIGAAYLIYLGIKLWRSPVGGEGADGQGVTGRERPLKIFLHAYI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHH
VTALNPKSIVFFVAFLPQFLVPTLPFWPQVLIFEATFLVLATVNAALYGLLASAARNTIR
HHHCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
KPKVQRIVNRTGGGLLIGAGLIAFGWKRAVSA
CHHHHHHHHCCCCCEEEHHHHHHHHHHHHCCC
>Mature Secondary Structure 
PLENWLAFVAASAIMLAIPGPTILLVISYALGHGRKASTATVTGVALGDFTAMTASMLG
CHHHHHHHHHHHHHHEECCCCHHHHHHHHHHCCCCCCCCCEEEEEHHHHHHHHHHHHHH
LGALLATSAALFTGLKWIGAAYLIYLGIKLWRSPVGGEGADGQGVTGRERPLKIFLHAYI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHH
VTALNPKSIVFFVAFLPQFLVPTLPFWPQVLIFEATFLVLATVNAALYGLLASAARNTIR
HHHCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
KPKVQRIVNRTGGGLLIGAGLIAFGWKRAVSA
CHHHHHHHHCCCCCEEEHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; homoserine [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + homoserine lactone [Cytoplasm] = Proton [Cytoplasm] + homoserine lactone [Periplasm] Proton [Periplasm] + homoserine [Cytoplasm] = Proton [Cytoplasm] + homoserine [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]