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The map label for this gene is nifS [H]

Identifier: 159184934

GI number: 159184934

Start: 1799744

End: 1800970

Strand: Reverse

Name: nifS [H]

Synonym: Atu1821

Alternate gene names: 159184934

Gene position: 1800970-1799744 (Counterclockwise)

Preceding gene: 159184935

Following gene: 159184933

Centisome position: 63.38

GC content: 59.58

Gene sequence:

>1227_bases
GTGGCGGCCCCTTATGACGTTGAAGCCGTGCGGAAGGATTTTCCAATCCTGTCGCGCGAGGTCTATGGCAAGCCGCTGAC
CTATCTCGACAATGGCGCGTCCGCTCAAAAACCGCAGGTGGTGATCGACGCCGTTTCGCATGCTTATTCCAACGAGTATG
CCAATGTGCATCGCGGCCTGCATTTCCTCTCCAATGCCGCCACCGACGCCTATGAGGCGGCGCGCGAAAAGGTGCGCCGT
TTCCTCAACGCCGGTTCGGTGGACGAGATAGTCTTCACCAAATCCTCCACCGAGGCGATCAATACGGTCGCTTACGGTTA
CGGCATGCCTGTTATCGGCGAGGGCGACGAGATCGTCATCTCGATCATGGAGCATCACTCCAACATCGTGCCATGGCATT
TCATCCGCGAACGGCAGGGCGCCAAGCTCGTCTGGGTGCCTGTTGATGATGACGGCGCGTTCCACATCGAGGCTTTCGAA
AAGAGCCTGACGGAGCGCACCAAGCTCGTTGCCATCACCCATATGTCGAATGCGCTCGGCACCATCTTGCCGGTCAAGGA
AATCTGCCGCATCGCCCATGAGCGCGGCATTCCGGTGCTGGTCGATGGTTCTCAGGGCGCGGTTCACATGCCGGTCGATG
TGCGTGATATCGATTGCGACTGGTATGTGATGACTGGCCACAAGCTTTACGGTCCCTCGGGCATCGGTGTGCTCTACGGC
AAGACTGAGCGCTTGCGCGAGATGCGGCCGTTCCAGGGCGGCGGTGAGATGATCCTCGACGTTTCCGAGGATAATGTGAC
CTATAACGAGCCGCCGCACCGTTTCGAAGCCGGCACCCCGCCCATCGTGCAGGCGATTGGCCTCGGTTACGCGCTGGATT
ATATGGACAATCTCGGCCGTGCCGCGATTGCAGCCCACGAGGCAGATCTCGCGGCTTATGCTGCGACACGACTGCGCGAG
GTCAACTCTCTGCGCATCATCGGCAATGCGCCTGACAAGGGCGGTATCTTCTCCTTCGAACTCGAGGGTATCCATGCCCA
CGACGTCTCGATGGTGATTGACCGGCGCGGCGTTGCCGTGCGTGCCGGCACCCATTGTGCCATGCCGCTCTTGAAACGGT
TCGGCGTCACCTCCACATGCCGGGCATCGTTCGGCCTTTACAATACCCGCGCCGAAGTAGATCGTCTTGTTGAGGCGCTG
GACTATGCGCGCAAATTTTTCGCCTGA

Upstream 100 bases:

>100_bases
GAAATCGTCGAGGAACTGGAAGAAGAGAATCTGGTAGAGGCGCTGGAGGCGATCATCTCCGGCTGGCTGGAACATCATGC
CTGAGAGCGAACGCGCCGCA

Downstream 100 bases:

>100_bases
TATCGGCATAGATGAGATCAGGATTGAGGATCAGACCATGACTGCGGAAGCCACTGCAAAGACACAAGATGCAACGGAAG
AAGCGGCAAAGCCGTCTTCC

Product: cysteine desulfurase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 408; Mature: 407

Protein sequence:

>408_residues
MAAPYDVEAVRKDFPILSREVYGKPLTYLDNGASAQKPQVVIDAVSHAYSNEYANVHRGLHFLSNAATDAYEAAREKVRR
FLNAGSVDEIVFTKSSTEAINTVAYGYGMPVIGEGDEIVISIMEHHSNIVPWHFIRERQGAKLVWVPVDDDGAFHIEAFE
KSLTERTKLVAITHMSNALGTILPVKEICRIAHERGIPVLVDGSQGAVHMPVDVRDIDCDWYVMTGHKLYGPSGIGVLYG
KTERLREMRPFQGGGEMILDVSEDNVTYNEPPHRFEAGTPPIVQAIGLGYALDYMDNLGRAAIAAHEADLAAYAATRLRE
VNSLRIIGNAPDKGGIFSFELEGIHAHDVSMVIDRRGVAVRAGTHCAMPLLKRFGVTSTCRASFGLYNTRAEVDRLVEAL
DYARKFFA

Sequences:

>Translated_408_residues
MAAPYDVEAVRKDFPILSREVYGKPLTYLDNGASAQKPQVVIDAVSHAYSNEYANVHRGLHFLSNAATDAYEAAREKVRR
FLNAGSVDEIVFTKSSTEAINTVAYGYGMPVIGEGDEIVISIMEHHSNIVPWHFIRERQGAKLVWVPVDDDGAFHIEAFE
KSLTERTKLVAITHMSNALGTILPVKEICRIAHERGIPVLVDGSQGAVHMPVDVRDIDCDWYVMTGHKLYGPSGIGVLYG
KTERLREMRPFQGGGEMILDVSEDNVTYNEPPHRFEAGTPPIVQAIGLGYALDYMDNLGRAAIAAHEADLAAYAATRLRE
VNSLRIIGNAPDKGGIFSFELEGIHAHDVSMVIDRRGVAVRAGTHCAMPLLKRFGVTSTCRASFGLYNTRAEVDRLVEAL
DYARKFFA
>Mature_407_residues
AAPYDVEAVRKDFPILSREVYGKPLTYLDNGASAQKPQVVIDAVSHAYSNEYANVHRGLHFLSNAATDAYEAAREKVRRF
LNAGSVDEIVFTKSSTEAINTVAYGYGMPVIGEGDEIVISIMEHHSNIVPWHFIRERQGAKLVWVPVDDDGAFHIEAFEK
SLTERTKLVAITHMSNALGTILPVKEICRIAHERGIPVLVDGSQGAVHMPVDVRDIDCDWYVMTGHKLYGPSGIGVLYGK
TERLREMRPFQGGGEMILDVSEDNVTYNEPPHRFEAGTPPIVQAIGLGYALDYMDNLGRAAIAAHEADLAAYAATRLREV
NSLRIIGNAPDKGGIFSFELEGIHAHDVSMVIDRRGVAVRAGTHCAMPLLKRFGVTSTCRASFGLYNTRAEVDRLVEALD
YARKFFA

Specific function: Catalyzes the removal of elemental sulfur and selenium atoms from L-cysteine, L-cystine, L-selenocysteine, and L- selenocystine to produce L-alanine [H]

COG id: COG0520

COG function: function code E; Selenocysteine lyase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-V pyridoxal-phosphate-dependent aminotransferase family. Csd subfamily [H]

Homologues:

Organism=Homo sapiens, GI32307132, Length=418, Percent_Identity=24.4019138755981, Blast_Score=109, Evalue=4e-24,
Organism=Homo sapiens, GI156713448, Length=426, Percent_Identity=27.6995305164319, Blast_Score=95, Evalue=1e-19,
Organism=Escherichia coli, GI1787970, Length=404, Percent_Identity=48.5148514851485, Blast_Score=390, Evalue=1e-110,
Organism=Escherichia coli, GI1789175, Length=397, Percent_Identity=40.3022670025189, Blast_Score=305, Evalue=3e-84,
Organism=Escherichia coli, GI48994898, Length=357, Percent_Identity=24.6498599439776, Blast_Score=100, Evalue=3e-22,
Organism=Caenorhabditis elegans, GI25143064, Length=230, Percent_Identity=30.4347826086957, Blast_Score=116, Evalue=2e-26,
Organism=Caenorhabditis elegans, GI193211090, Length=374, Percent_Identity=25.9358288770053, Blast_Score=96, Evalue=3e-20,
Organism=Caenorhabditis elegans, GI17533177, Length=332, Percent_Identity=25.6024096385542, Blast_Score=67, Evalue=1e-11,
Organism=Saccharomyces cerevisiae, GI6319831, Length=400, Percent_Identity=25, Blast_Score=105, Evalue=2e-23,
Organism=Drosophila melanogaster, GI20129463, Length=278, Percent_Identity=29.8561151079137, Blast_Score=122, Evalue=5e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000192
- InterPro:   IPR020578
- InterPro:   IPR010970
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF00266 Aminotran_5 [H]

EC number: =2.8.1.7 [H]

Molecular weight: Translated: 44975; Mature: 44843

Theoretical pI: Translated: 6.20; Mature: 6.20

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAAPYDVEAVRKDFPILSREVYGKPLTYLDNGASAQKPQVVIDAVSHAYSNEYANVHRGL
CCCCCCHHHHHHCCCHHHHHHCCCCCEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
HFLSNAATDAYEAAREKVRRFLNAGSVDEIVFTKSSTEAINTVAYGYGMPVIGEGDEIVI
HHHHHCCHHHHHHHHHHHHHHHCCCCCCEEEEECCCHHHHHHHHHHCCCCEECCCCCEEE
SIMEHHSNIVPWHFIRERQGAKLVWVPVDDDGAFHIEAFEKSLTERTKLVAITHMSNALG
EEEHHCCCCCCHHHHHHCCCCEEEEEEECCCCCEEHHHHHHHHHHHHEEEEEEECCHHHH
TILPVKEICRIAHERGIPVLVDGSQGAVHMPVDVRDIDCDWYVMTGHKLYGPSGIGVLYG
HHCCHHHHHHHHHHCCCEEEEECCCCCEECCEEEEECCCCEEEEECCEEECCCCCEEEEC
KTERLREMRPFQGGGEMILDVSEDNVTYNEPPHRFEAGTPPIVQAIGLGYALDYMDNLGR
CHHHHHHCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCH
AAIAAHEADLAAYAATRLREVNSLRIIGNAPDKGGIFSFELEGIHAHDVSMVIDRRGVAV
HHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCEEEEEECCEEECCCEEEEECCCEEE
RAGTHCAMPLLKRFGVTSTCRASFGLYNTRAEVDRLVEALDYARKFFA
ECCCHHHHHHHHHCCCCHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
AAPYDVEAVRKDFPILSREVYGKPLTYLDNGASAQKPQVVIDAVSHAYSNEYANVHRGL
CCCCCHHHHHHCCCHHHHHHCCCCCEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
HFLSNAATDAYEAAREKVRRFLNAGSVDEIVFTKSSTEAINTVAYGYGMPVIGEGDEIVI
HHHHHCCHHHHHHHHHHHHHHHCCCCCCEEEEECCCHHHHHHHHHHCCCCEECCCCCEEE
SIMEHHSNIVPWHFIRERQGAKLVWVPVDDDGAFHIEAFEKSLTERTKLVAITHMSNALG
EEEHHCCCCCCHHHHHHCCCCEEEEEEECCCCCEEHHHHHHHHHHHHEEEEEEECCHHHH
TILPVKEICRIAHERGIPVLVDGSQGAVHMPVDVRDIDCDWYVMTGHKLYGPSGIGVLYG
HHCCHHHHHHHHHHCCCEEEEECCCCCEECCEEEEECCCCEEEEECCEEECCCCCEEEEC
KTERLREMRPFQGGGEMILDVSEDNVTYNEPPHRFEAGTPPIVQAIGLGYALDYMDNLGR
CHHHHHHCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCH
AAIAAHEADLAAYAATRLREVNSLRIIGNAPDKGGIFSFELEGIHAHDVSMVIDRRGVAV
HHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCEEEEEECCEEECCCEEEEECCCEEE
RAGTHCAMPLLKRFGVTSTCRASFGLYNTRAEVDRLVEALDYARKFFA
ECCCHHHHHHHHHCCCCHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11058132 [H]