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The map label for this gene is pcm [H]

Identifier: 159184877

GI number: 159184877

Start: 1686722

End: 1687363

Strand: Reverse

Name: pcm [H]

Synonym: Atu1701

Alternate gene names: 159184877

Gene position: 1687363-1686722 (Counterclockwise)

Preceding gene: 15889009

Following gene: 159184876

Centisome position: 59.38

GC content: 61.37

Gene sequence:

>642_bases
ATGGTCGAAAAGGAAGGTTTTGCCGCTCTCGTTCTCCGTCTGCGGGGCGAGGGGATTTCCGATCTCGACCTGTTGACGGC
GGTTGAGCAGACGCCGCGTTCCAAATTCGTGCCGCCGCAATTTGCGGCCGACGCCTATTCCAGCCGCACGATCCCGATCG
ACTGCGGTTCCTTCATGGAAGGCGCCGACATGGCGGTGAAAATCCTTGCCCGCCTGCAGCTGAAACCCGGCCAGCGCGTG
CTTGAGATCGGCACCGGCAGCGGTTTTATGACGGCGATCATCGCGCGCCGGGTGGAGCGGGTGTTTTCGCTGGAGCGTTA
CAAGACGCTGGTGCAGCAGGCGCAGAACTGTCTCGACGATCTGTCCATCCGCAACGTCGTCATCCGCCAGGCGGATGGCA
GCAATGGTCTGGTGGGCGAGGGCACCTTCGACCGGATTGTTTCCACCGCCGCCTTTACCACCATGCCCCGCTTCTTTGCC
GAGCAGATCGTTTCCGGCGGCATGATGATCGCGCCGATCATCCTGGAAGATGAGCGTTGCGTGATGACCCGCTTCTCCAA
GACCGGCAGCCGCTTCGAGAAGGAAGAGCTGTTCGAGACGCCGTATCTGCCACTCGGCAGCCATATTGCGCGGCATTTGT
GA

Upstream 100 bases:

>100_bases
AGATTTCGGTCACACCGCTTAAGCTTGACCTGACGGACTACACGGTCAAGGATCGGGTTGCGCAAGCACTTGGATTCGGA
GTCGCCGATTGAAATCTGCC

Downstream 100 bases:

>100_bases
CGAAATGAAAATTTTGAAGGCAATTCATTTTTGTCTTCTAATGTTCTGAATTGTCGCAACTATCTGATTTTCAATATCAT
TATTGTCTCGACATGACGCA

Product: protein-L-isoaspartate O-methyltransferase

Products: NA

Alternate protein names: L-isoaspartyl protein carboxyl methyltransferase; Protein L-isoaspartyl methyltransferase; Protein-beta-aspartate methyltransferase; PIMT [H]

Number of amino acids: Translated: 213; Mature: 213

Protein sequence:

>213_residues
MVEKEGFAALVLRLRGEGISDLDLLTAVEQTPRSKFVPPQFAADAYSSRTIPIDCGSFMEGADMAVKILARLQLKPGQRV
LEIGTGSGFMTAIIARRVERVFSLERYKTLVQQAQNCLDDLSIRNVVIRQADGSNGLVGEGTFDRIVSTAAFTTMPRFFA
EQIVSGGMMIAPIILEDERCVMTRFSKTGSRFEKEELFETPYLPLGSHIARHL

Sequences:

>Translated_213_residues
MVEKEGFAALVLRLRGEGISDLDLLTAVEQTPRSKFVPPQFAADAYSSRTIPIDCGSFMEGADMAVKILARLQLKPGQRV
LEIGTGSGFMTAIIARRVERVFSLERYKTLVQQAQNCLDDLSIRNVVIRQADGSNGLVGEGTFDRIVSTAAFTTMPRFFA
EQIVSGGMMIAPIILEDERCVMTRFSKTGSRFEKEELFETPYLPLGSHIARHL
>Mature_213_residues
MVEKEGFAALVLRLRGEGISDLDLLTAVEQTPRSKFVPPQFAADAYSSRTIPIDCGSFMEGADMAVKILARLQLKPGQRV
LEIGTGSGFMTAIIARRVERVFSLERYKTLVQQAQNCLDDLSIRNVVIRQADGSNGLVGEGTFDRIVSTAAFTTMPRFFA
EQIVSGGMMIAPIILEDERCVMTRFSKTGSRFEKEELFETPYLPLGSHIARHL

Specific function: Catalyzes the methyl esterification of L-isoaspartyl residues in peptides and proteins that result from spontaneous decomposition of normal L-aspartyl and L-asparaginyl residues. It plays a role in the repair and/or degradation of damaged proteins [H]

COG id: COG2518

COG function: function code O; Protein-L-isoaspartate carboxylmethyltransferase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. L- isoaspartyl/D-aspartyl protein methyltransferase family [H]

Homologues:

Organism=Escherichia coli, GI1789100, Length=197, Percent_Identity=36.0406091370558, Blast_Score=123, Evalue=1e-29,
Organism=Drosophila melanogaster, GI17981723, Length=213, Percent_Identity=26.7605633802817, Blast_Score=69, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000682 [H]

Pfam domain/function: PF01135 PCMT [H]

EC number: =2.1.1.77 [H]

Molecular weight: Translated: 23580; Mature: 23580

Theoretical pI: Translated: 6.54; Mature: 6.54

Prosite motif: PS01279 PCMT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVEKEGFAALVLRLRGEGISDLDLLTAVEQTPRSKFVPPQFAADAYSSRTIPIDCGSFME
CCCCHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCCCCHHHHHHHCCCCEEECCCCHHC
GADMAVKILARLQLKPGQRVLEIGTGSGFMTAIIARRVERVFSLERYKTLVQQAQNCLDD
HHHHHHHHHHHHCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LSIRNVVIRQADGSNGLVGEGTFDRIVSTAAFTTMPRFFAEQIVSGGMMIAPIILEDERC
HHHHHHHEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEECCCHH
VMTRFSKTGSRFEKEELFETPYLPLGSHIARHL
HHHHHHHHCCHHHHHHHHCCCCCCCHHHHHHCC
>Mature Secondary Structure
MVEKEGFAALVLRLRGEGISDLDLLTAVEQTPRSKFVPPQFAADAYSSRTIPIDCGSFME
CCCCHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCCCCHHHHHHHCCCCEEECCCCHHC
GADMAVKILARLQLKPGQRVLEIGTGSGFMTAIIARRVERVFSLERYKTLVQQAQNCLDD
HHHHHHHHHHHHCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LSIRNVVIRQADGSNGLVGEGTFDRIVSTAAFTTMPRFFAEQIVSGGMMIAPIILEDERC
HHHHHHHEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEECCCHH
VMTRFSKTGSRFEKEELFETPYLPLGSHIARHL
HHHHHHHHCCHHHHHHHHCCCCCCCHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9304864; 11481430 [H]