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The map label for this gene is queA

Identifier: 159184871

GI number: 159184871

Start: 1665675

End: 1666775

Strand: Reverse

Name: queA

Synonym: Atu1678

Alternate gene names: 159184871

Gene position: 1666775-1665675 (Counterclockwise)

Preceding gene: 15888985

Following gene: 15888983

Centisome position: 58.66

GC content: 60.58

Gene sequence:

>1101_bases
ATGCGTGTAGACCTGTTCGATTTCGACCTGCCCGAGGAGAATATCGCCCTTCGCCCGGCGAACCCGCGCGATAGCGCGCG
CCTGCTGGTGGTCGATCCGAATGAAAACCGCATGGAAGACCATCGCGTCTTCGACCTGCCGTCGTTCCTGAGGCCCGGTG
ATGCGCTTGTCTTCAACGACACCCGTGTCATTCCCGCCCAGCTGGAAGGCGTGCGCCTGCGCGAAGGTGCGCCGGAAACG
GCGGTGTCGGCGACGCTGCATATGCGTGCCGATCAATCCCGCTGGAAGGCTTTCGCGCGGCCCGGCAAGCGCATCAAAGA
AGGCGACCGTATCCGTTTCGGTTATGACCGCGACACTGTTAATTCATCCAGCGGGGCCTGCGGTCTTGCCCATCTCGAAG
CCACCGTCGAGGAAAAGGGCGAGGAGGGCGAGATCACGCTGCTTTTCGACATTTCCGGCCCGGTGCTGGATGAGGCGATA
GCCTCTGTTGGCCATATTCCTTTGCCGCCTTACATCGCCGCCAAGCGGCCCGAGGACCAGCAGGACCAGACCGACTATCA
GACCATTTACGCCCGCGAGAAGGGTGCGGTTGCCGCCCCCACCGCCGGGCTGCATTTCACCCCCGATCTGTTCGAAGCGC
TCGACAGTGCCGGCATCGAACGGCATTTCGTGACGCTTCACGTTGGGGCAGGCACCTTCCTCCCGGTGAAGTCCGATGAT
ACCGACGATCACAAGATGCATTTCGAGATCGGGTATGTGTCGCAAGAGACTGCTGACCGGCTGAATGCGGTGAAGGCGCG
GGGCGGGCGCATCGTCTGCGTCGGTACCACCTCGCTGCGCCTGATCGAAAGTGCGGCCACCGAAGATGGCACCATCCACC
CATGGTCCGATGCCACCGGCATCTTCATCACGCCCGGATATCGTTTCCGCGCTGTCGATATGCTGATGACCAATTTCCAT
CTGCCGAAATCCACGCTGTTCATGCTGGTTTCGGCTTTCTGCGGTCTCGAAACGATGCGTGACGCTTACAGGCACGCCAT
CGAAACCGGATACCGCTTCTATTCCTATGGCGATTCCAGCCTGTTGTTCCGGAAAAACTGA

Upstream 100 bases:

>100_bases
AAGCCCGAGGATGACGTCGAGTGTAGAAGCACCCTGTCACCACCTTCCACCCGCCCCATGCCATGCAGGGCGGGTTTTGA
TATTTTCGAGAGCCCTTGCA

Downstream 100 bases:

>100_bases
TGCACGAGAAATTCACCTTCACCCTGAAATCCACGAGCGGCGGCGCGCGCCTTGGCGAAGTCGCCATGCCGCGCGGTGTC
ATCCGCACGCCCGCCTTCAT

Product: S-adenosylmethionine:tRNA ribosyltransferase-isomerase

Products: NA

Alternate protein names: Queuosine biosynthesis protein queA

Number of amino acids: Translated: 366; Mature: 366

Protein sequence:

>366_residues
MRVDLFDFDLPEENIALRPANPRDSARLLVVDPNENRMEDHRVFDLPSFLRPGDALVFNDTRVIPAQLEGVRLREGAPET
AVSATLHMRADQSRWKAFARPGKRIKEGDRIRFGYDRDTVNSSSGACGLAHLEATVEEKGEEGEITLLFDISGPVLDEAI
ASVGHIPLPPYIAAKRPEDQQDQTDYQTIYAREKGAVAAPTAGLHFTPDLFEALDSAGIERHFVTLHVGAGTFLPVKSDD
TDDHKMHFEIGYVSQETADRLNAVKARGGRIVCVGTTSLRLIESAATEDGTIHPWSDATGIFITPGYRFRAVDMLMTNFH
LPKSTLFMLVSAFCGLETMRDAYRHAIETGYRFYSYGDSSLLFRKN

Sequences:

>Translated_366_residues
MRVDLFDFDLPEENIALRPANPRDSARLLVVDPNENRMEDHRVFDLPSFLRPGDALVFNDTRVIPAQLEGVRLREGAPET
AVSATLHMRADQSRWKAFARPGKRIKEGDRIRFGYDRDTVNSSSGACGLAHLEATVEEKGEEGEITLLFDISGPVLDEAI
ASVGHIPLPPYIAAKRPEDQQDQTDYQTIYAREKGAVAAPTAGLHFTPDLFEALDSAGIERHFVTLHVGAGTFLPVKSDD
TDDHKMHFEIGYVSQETADRLNAVKARGGRIVCVGTTSLRLIESAATEDGTIHPWSDATGIFITPGYRFRAVDMLMTNFH
LPKSTLFMLVSAFCGLETMRDAYRHAIETGYRFYSYGDSSLLFRKN
>Mature_366_residues
MRVDLFDFDLPEENIALRPANPRDSARLLVVDPNENRMEDHRVFDLPSFLRPGDALVFNDTRVIPAQLEGVRLREGAPET
AVSATLHMRADQSRWKAFARPGKRIKEGDRIRFGYDRDTVNSSSGACGLAHLEATVEEKGEEGEITLLFDISGPVLDEAI
ASVGHIPLPPYIAAKRPEDQQDQTDYQTIYAREKGAVAAPTAGLHFTPDLFEALDSAGIERHFVTLHVGAGTFLPVKSDD
TDDHKMHFEIGYVSQETADRLNAVKARGGRIVCVGTTSLRLIESAATEDGTIHPWSDATGIFITPGYRFRAVDMLMTNFH
LPKSTLFMLVSAFCGLETMRDAYRHAIETGYRFYSYGDSSLLFRKN

Specific function: Transfers and isomerizes the ribose moiety from AdoMet to the 7-aminomethyl group of 7-deazaguanine (preQ1-tRNA) to give epoxyqueuosine (oQ-tRNA)

COG id: COG0809

COG function: function code J; S-adenosylmethionine:tRNA-ribosyltransferase-isomerase (queuine synthetase)

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the queA family

Homologues:

Organism=Escherichia coli, GI1786606, Length=364, Percent_Identity=45.6043956043956, Blast_Score=278, Evalue=5e-76,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): QUEA_AGRT5 (Q8UES7)

Other databases:

- EMBL:   AE007869
- PIR:   A97562
- PIR:   AH2782
- RefSeq:   NP_354665.2
- ProteinModelPortal:   Q8UES7
- SMR:   Q8UES7
- STRING:   Q8UES7
- GeneID:   1133716
- GenomeReviews:   AE007869_GR
- KEGG:   atu:Atu1678
- eggNOG:   COG0809
- HOGENOM:   HBG286076
- OMA:   KARIHEA
- PhylomeDB:   Q8UES7
- ProtClustDB:   PRK00147
- BioCyc:   ATUM176299-1:ATU1678-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00113
- InterPro:   IPR003699
- TIGRFAMs:   TIGR00113

Pfam domain/function: PF02547 Queuosine_synth; SSF111337 Queuosine_synth

EC number: 5.-.-.- [C]

Molecular weight: Translated: 40719; Mature: 40719

Theoretical pI: Translated: 5.29; Mature: 5.29

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRVDLFDFDLPEENIALRPANPRDSARLLVVDPNENRMEDHRVFDLPSFLRPGDALVFND
CEEEEEECCCCCCCEEEECCCCCCCCEEEEECCCCCCCCCCEEECCHHHCCCCCEEEEEC
TRVIPAQLEGVRLREGAPETAVSATLHMRADQSRWKAFARPGKRIKEGDRIRFGYDRDTV
CEEEECCCCCEEECCCCCCHHHEEEEEECCCHHHHHHHHCCCCCCCCCCEEEECCCCCCC
NSSSGACGLAHLEATVEEKGEEGEITLLFDISGPVLDEAIASVGHIPLPPYIAAKRPEDQ
CCCCCCCCHHHHHHHHHHCCCCCCEEEEEECCCHHHHHHHHHCCCCCCCCCEECCCCCCC
QDQTDYQTIYAREKGAVAAPTAGLHFTPDLFEALDSAGIERHFVTLHVGAGTFLPVKSDD
CCCCHHHEEEEECCCCEECCCCCCCCCHHHHHHHHHCCCEEEEEEEEECCCEEEEECCCC
TDDHKMHFEIGYVSQETADRLNAVKARGGRIVCVGTTSLRLIESAATEDGTIHPWSDATG
CCCCEEEEEEEECCHHHHHHHHHHHCCCCEEEEEECCHHHHHHHHCCCCCCEECCCCCCE
IFITPGYRFRAVDMLMTNFHLPKSTLFMLVSAFCGLETMRDAYRHAIETGYRFYSYGDSS
EEECCCCCEEEHHHHHHHCCCCHHHHHHHHHHHHCHHHHHHHHHHHHHCCCEEEEECCCE
LLFRKN
EEEECC
>Mature Secondary Structure
MRVDLFDFDLPEENIALRPANPRDSARLLVVDPNENRMEDHRVFDLPSFLRPGDALVFND
CEEEEEECCCCCCCEEEECCCCCCCCEEEEECCCCCCCCCCEEECCHHHCCCCCEEEEEC
TRVIPAQLEGVRLREGAPETAVSATLHMRADQSRWKAFARPGKRIKEGDRIRFGYDRDTV
CEEEECCCCCEEECCCCCCHHHEEEEEECCCHHHHHHHHCCCCCCCCCCEEEECCCCCCC
NSSSGACGLAHLEATVEEKGEEGEITLLFDISGPVLDEAIASVGHIPLPPYIAAKRPEDQ
CCCCCCCCHHHHHHHHHHCCCCCCEEEEEECCCHHHHHHHHHCCCCCCCCCEECCCCCCC
QDQTDYQTIYAREKGAVAAPTAGLHFTPDLFEALDSAGIERHFVTLHVGAGTFLPVKSDD
CCCCHHHEEEEECCCCEECCCCCCCCCHHHHHHHHHCCCEEEEEEEEECCCEEEEECCCC
TDDHKMHFEIGYVSQETADRLNAVKARGGRIVCVGTTSLRLIESAATEDGTIHPWSDATG
CCCCEEEEEEEECCHHHHHHHHHHHCCCCEEEEEECCHHHHHHHHCCCCCCEECCCCCCE
IFITPGYRFRAVDMLMTNFHLPKSTLFMLVSAFCGLETMRDAYRHAIETGYRFYSYGDSS
EEECCCCCEEEHHHHHHHCCCCHHHHHHHHHHHHCHHHHHHHHHHHHHCCCEEEEECCCE
LLFRKN
EEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Isomerases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11743193; 11743194