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The map label for this gene is gidA

Identifier: 159184867

GI number: 159184867

Start: 1654279

End: 1655724

Strand: Direct

Name: gidA

Synonym: Atu1669

Alternate gene names: 159184867

Gene position: 1654279-1655724 (Clockwise)

Preceding gene: 15888973

Following gene: 15888975

Centisome position: 58.22

GC content: 62.86

Gene sequence:

>1446_bases
ATGCAAGACAAGACCCATTCTCCCATTCACGTCGTGGGCGGCGGACTGGCCGGCTCGGAAGCCGCATGGCAGATTGCTGA
GAGCGGCGTGCCCGTCATCCTGCACGAAATGCGCGGCGTGCGCGGCACGGATGCGCATAAGGGCGATACGCTGGCGGAAC
TGGTCTGTTCCAACTCCTTCCGCTCCGACGACGCGACGGCCAATGCGGTCGGCGTCATCCATGCGGAAATGCGGCTTGCC
GGCTCGCTGATCATCGCCTGCGCCGACAAACACCAGGTGCCGGCCGGCGGCGCACTGGCCGTCGACCGTGACGGGTTTTC
GCAGGCCGTGACGGATATGCTTGAAAATCACCCATTGGTGACAGTTGTCCGCGAAGAAGTAACAGGCCTGCCCCCGAAGG
AATGGGGCAGCACCGTCATCGCCACCGGCCCGCTCACCTCACCGGATCTGGCAGCAGCGGTTCAGGCTGAGACGGGCGAA
GATGCGCTGGCATTCTTTGACGCCATTGCCCCCATCCTGCACCGCGACAGCATCAACATGGATATCTGCTGGTACCAGTC
GCGTTACGACAAGGTCGGCCCCGGCGGCACGGGCAAGGATTATATCAACTGCCCGATGGATGAGGAACAGTACAACGTCT
TCATCGACGCGCTGATCGCCGGCGACACGGTCGGTTTTAAGGAATGGGAAGGCACGCCCTATTTCGACGGCTGCCTGCCG
ATCGAGATCATGGCGGAGCGCGGCCGTCAGACGCTGCGCCACGGCCCGATGAAGCCGATGGGCCTCACCAACGCCCATAA
CCCGACCGTTAAAGCCTATGCGGTCGTCCAGTTGCGGCAGGACAATGCGCTCGGCACGCTCTACAACATGGTCGGTTTCC
AGACCAAGCTGAAATACAGTGTGCAGGCGGACGTGTTCCGCATGATTCCGGGTCTGGAAAATGCGGAATTCGCGCGCCTA
GGCGGCCTGCACCGCAATACCTATATCGATTCTCCCATCCTGCTCGACCGCTCGCTGAGGCTGAAATCGCGGCCCGACCT
GCGTTTTGCCGGCCAGATTACCGGCTGCGAAGGGTATGTGGAAAGCGCCTCCGTCGGGCTGCTCGCCGGCCGCTTCGCCG
CTGCGGAGCGCAAGGGCGAGGCCCCGAGCCTGCCGCCGGCCACCACGGCGCTGGGCTCGCTCCTCAACCACATCACCGGC
GGCCACCTCTCATCCGATGATGAGCCGGGCAAGCGTTCCTTCCAGCCGATGAACATCAATTTCGGCCTGTTCCCGGAACT
GGAGCCGGGCTCCATCGTCAAGCCTGATGGTGTGAAACGTTTTCGCGGCAAGGACAAGACGATCATGAAACGCCAGCTGG
TTGCAGCGCGGGCGCTGAAGGATTGTGCGGCGTGGCTTGGCGTGGAACGCGTCGCAACGGAACAAGAGAGCGACGCAACG
CCATAG

Upstream 100 bases:

>100_bases
CGCAACAATGCCGCGTCCCCGAGGGACCCGCGCGAACCATCACTCGAATGACAGCCGCCGGACGGGAGCTTTGCCCCGCG
TGCGGTTCATGGACGCCAGT

Downstream 100 bases:

>100_bases
GCCCTGCCCGTATCTATCAGCCAGAATAAAGGGGCGTGTGACCGCACACGCCCTTTTTCCATTCCGGGTCACCGGATTTT
GCCTTGCCCGGCCAGCCGCG

Product: tRNA (uracil-5-)-methyltransferase Gid

Products: NA

Alternate protein names: Folate-dependent tRNA (uracil-5-)-methyltransferase; Folate-dependent tRNA(M-5-U54)-methyltransferase

Number of amino acids: Translated: 481; Mature: 481

Protein sequence:

>481_residues
MQDKTHSPIHVVGGGLAGSEAAWQIAESGVPVILHEMRGVRGTDAHKGDTLAELVCSNSFRSDDATANAVGVIHAEMRLA
GSLIIACADKHQVPAGGALAVDRDGFSQAVTDMLENHPLVTVVREEVTGLPPKEWGSTVIATGPLTSPDLAAAVQAETGE
DALAFFDAIAPILHRDSINMDICWYQSRYDKVGPGGTGKDYINCPMDEEQYNVFIDALIAGDTVGFKEWEGTPYFDGCLP
IEIMAERGRQTLRHGPMKPMGLTNAHNPTVKAYAVVQLRQDNALGTLYNMVGFQTKLKYSVQADVFRMIPGLENAEFARL
GGLHRNTYIDSPILLDRSLRLKSRPDLRFAGQITGCEGYVESASVGLLAGRFAAAERKGEAPSLPPATTALGSLLNHITG
GHLSSDDEPGKRSFQPMNINFGLFPELEPGSIVKPDGVKRFRGKDKTIMKRQLVAARALKDCAAWLGVERVATEQESDAT
P

Sequences:

>Translated_481_residues
MQDKTHSPIHVVGGGLAGSEAAWQIAESGVPVILHEMRGVRGTDAHKGDTLAELVCSNSFRSDDATANAVGVIHAEMRLA
GSLIIACADKHQVPAGGALAVDRDGFSQAVTDMLENHPLVTVVREEVTGLPPKEWGSTVIATGPLTSPDLAAAVQAETGE
DALAFFDAIAPILHRDSINMDICWYQSRYDKVGPGGTGKDYINCPMDEEQYNVFIDALIAGDTVGFKEWEGTPYFDGCLP
IEIMAERGRQTLRHGPMKPMGLTNAHNPTVKAYAVVQLRQDNALGTLYNMVGFQTKLKYSVQADVFRMIPGLENAEFARL
GGLHRNTYIDSPILLDRSLRLKSRPDLRFAGQITGCEGYVESASVGLLAGRFAAAERKGEAPSLPPATTALGSLLNHITG
GHLSSDDEPGKRSFQPMNINFGLFPELEPGSIVKPDGVKRFRGKDKTIMKRQLVAARALKDCAAWLGVERVATEQESDAT
P
>Mature_481_residues
MQDKTHSPIHVVGGGLAGSEAAWQIAESGVPVILHEMRGVRGTDAHKGDTLAELVCSNSFRSDDATANAVGVIHAEMRLA
GSLIIACADKHQVPAGGALAVDRDGFSQAVTDMLENHPLVTVVREEVTGLPPKEWGSTVIATGPLTSPDLAAAVQAETGE
DALAFFDAIAPILHRDSINMDICWYQSRYDKVGPGGTGKDYINCPMDEEQYNVFIDALIAGDTVGFKEWEGTPYFDGCLP
IEIMAERGRQTLRHGPMKPMGLTNAHNPTVKAYAVVQLRQDNALGTLYNMVGFQTKLKYSVQADVFRMIPGLENAEFARL
GGLHRNTYIDSPILLDRSLRLKSRPDLRFAGQITGCEGYVESASVGLLAGRFAAAERKGEAPSLPPATTALGSLLNHITG
GHLSSDDEPGKRSFQPMNINFGLFPELEPGSIVKPDGVKRFRGKDKTIMKRQLVAARALKDCAAWLGVERVATEQESDAT
P

Specific function: Catalyzes the folate-dependent formation of 5-methyl- uridine at position 54 (M-5-U54) in all tRNAs

COG id: COG1206

COG function: function code J; NAD(FAD)-utilizing enzyme possibly involved in translation

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MnmG family. TrmFO subfamily

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TRMFO_AGRT5 (Q8UET6)

Other databases:

- EMBL:   AE007869
- PIR:   AG2781
- PIR:   G97560
- RefSeq:   NP_354655.2
- ProteinModelPortal:   Q8UET6
- STRING:   Q8UET6
- GeneID:   1133707
- GenomeReviews:   AE007869_GR
- KEGG:   atu:Atu1669
- eggNOG:   COG1206
- HOGENOM:   HBG285041
- OMA:   FEGCLPI
- ProtClustDB:   PRK05335
- BioCyc:   ATUM176299-1:ATU1669-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01037
- InterPro:   IPR004417
- InterPro:   IPR002218
- InterPro:   IPR020595
- TIGRFAMs:   TIGR00137

Pfam domain/function: PF01134 GIDA

EC number: =2.1.1.74

Molecular weight: Translated: 51801; Mature: 51801

Theoretical pI: Translated: 5.61; Mature: 5.61

Prosite motif: PS01280 GIDA_1; PS01281 GIDA_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQDKTHSPIHVVGGGLAGSEAAWQIAESGVPVILHEMRGVRGTDAHKGDTLAELVCSNSF
CCCCCCCCEEEEECCCCCCHHHHHHHHCCCCEEEHHHCCCCCCCCCCCCHHHHHHHCCCC
RSDDATANAVGVIHAEMRLAGSLIIACADKHQVPAGGALAVDRDGFSQAVTDMLENHPLV
CCCCCCCCEEEEEEHHHHHCCEEEEEECCCCCCCCCCEEEECCCCHHHHHHHHHHCCCEE
TVVREEVTGLPPKEWGSTVIATGPLTSPDLAAAVQAETGEDALAFFDAIAPILHRDSINM
EEEEHHHCCCCCHHHCCEEEEECCCCCCCHHHEEECCCCCHHHHHHHHHHHHHHCCCCCE
DICWYQSRYDKVGPGGTGKDYINCPMDEEQYNVFIDALIAGDTVGFKEWEGTPYFDGCLP
EEEEECCCCCCCCCCCCCCCCEECCCCCCHHEEEEEEEEECCCCCCCCCCCCCCCCCCCH
IEIMAERGRQTLRHGPMKPMGLTNAHNPTVKAYAVVQLRQDNALGTLYNMVGFQTKLKYS
HHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEEEEEECCCCHHHHHHHHCCHHEEEEE
VQADVFRMIPGLENAEFARLGGLHRNTYIDSPILLDRSLRLKSRPDLRFAGQITGCEGYV
HHHHHHHHCCCCCCCCHHHHCCCCCCCCCCCCEEECCCCCCCCCCCCEEEEEECCCCCHH
ESASVGLLAGRFAAAERKGEAPSLPPATTALGSLLNHITGGHLSSDDEPGKRSFQPMNIN
HCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEECC
FGLFPELEPGSIVKPDGVKRFRGKDKTIMKRQLVAARALKDCAAWLGVERVATEQESDAT
CCCCCCCCCCCEECCCCHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
P
C
>Mature Secondary Structure
MQDKTHSPIHVVGGGLAGSEAAWQIAESGVPVILHEMRGVRGTDAHKGDTLAELVCSNSF
CCCCCCCCEEEEECCCCCCHHHHHHHHCCCCEEEHHHCCCCCCCCCCCCHHHHHHHCCCC
RSDDATANAVGVIHAEMRLAGSLIIACADKHQVPAGGALAVDRDGFSQAVTDMLENHPLV
CCCCCCCCEEEEEEHHHHHCCEEEEEECCCCCCCCCCEEEECCCCHHHHHHHHHHCCCEE
TVVREEVTGLPPKEWGSTVIATGPLTSPDLAAAVQAETGEDALAFFDAIAPILHRDSINM
EEEEHHHCCCCCHHHCCEEEEECCCCCCCHHHEEECCCCCHHHHHHHHHHHHHHCCCCCE
DICWYQSRYDKVGPGGTGKDYINCPMDEEQYNVFIDALIAGDTVGFKEWEGTPYFDGCLP
EEEEECCCCCCCCCCCCCCCCEECCCCCCHHEEEEEEEEECCCCCCCCCCCCCCCCCCCH
IEIMAERGRQTLRHGPMKPMGLTNAHNPTVKAYAVVQLRQDNALGTLYNMVGFQTKLKYS
HHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEEEEEECCCCHHHHHHHHCCHHEEEEE
VQADVFRMIPGLENAEFARLGGLHRNTYIDSPILLDRSLRLKSRPDLRFAGQITGCEGYV
HHHHHHHHCCCCCCCCHHHHCCCCCCCCCCCCEEECCCCCCCCCCCCEEEEEECCCCCHH
ESASVGLLAGRFAAAERKGEAPSLPPATTALGSLLNHITGGHLSSDDEPGKRSFQPMNIN
HCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEECC
FGLFPELEPGSIVKPDGVKRFRGKDKTIMKRQLVAARALKDCAAWLGVERVATEQESDAT
CCCCCCCCCCCEECCCCHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
P
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11743193; 11743194