Click here to switch to the map view.

The map label for this gene is lipB

Identifier: 159184811

GI number: 159184811

Start: 1543672

End: 1544373

Strand: Direct

Name: lipB

Synonym: Atu1556

Alternate gene names: 159184811

Gene position: 1543672-1544373 (Clockwise)

Preceding gene: 159184810

Following gene: 159184812

Centisome position: 54.32

GC content: 63.25

Gene sequence:

>702_bases
ATGTTCCCCGCCGCCGGATCACCACCCGTTCGCTGGCGAATCTCGGAAGGCCTCGTCGCTTACGGGCAGGCTGTCGAGGA
GATGGAGCGTGAGGTGGCCGCTATCGCCGCCGGCGAGGCCGACGAACTCGTCTGGCTGCTGGAGCATCCTCCGCTTTATA
CCGCCGGCACCAGTGCCGATGTAGCCGACCTCATCGAGCCGGACCGTTTTCCCGTCTTCGCCACGGGTCGCGGCGGCGAA
TATACCTATCACGGCCCCGGCCAGCGGGTTGTTTATGTCATGCTCGACCTGAAACGGCGGCGGCAGGATGTGCGCGCCTA
TGTGGCGGCGCTGGAGGATGTCATCATCCGCACGCTCGACAAGATGAATGTCCGTGGCGAGCGGCGTGAGGATCGCGTCG
GTGTCTGGGTCAGGCGGCCGGAAAAACCTCTTTTGCCGGATGGCGGCATGGCAGAGGACAAGATCGCAGCGCTCGGCATT
CGTCTGCGCAAATGGGTGAGCTTTCACGGACTGTCGATCAATGTGGATCCCGACCTCTCGCATTTCTCGGGCATCGTTCC
CTGCGGCATCAGTGCCTATGGCGTCACCAGCCTTGTCGATCTTGGATTGCCGGTTATGATCGGCGACGTCGACGTTCTGC
TGCGCGAGGCCTTTGAGGAGGTTTTTGGCCCGGCAGTGCCCGAAACCGGCGCAGGCGGCTGA

Upstream 100 bases:

>100_bases
GGAAGCCCGGAAATGCTGGGGTTGCGGCCTTGTCGCCATTTTGCGAGAAGGCTGTGGAAAGGACTTTTTCATGCTCACAC
GCACGGATATCGAGACAAAT

Downstream 100 bases:

>100_bases
TCCCACCGCAACCGTTGTCGCAGGCCGGTGTTTGATGCATCGATCACCATAAGAGGAGGAACGACCATGTTCACAACGTC
CGCCTATGCCTGCGATGACG

Product: lipoate-protein ligase B

Products: NA

Alternate protein names: Lipoate-protein ligase B; Lipoyl/octanoyl transferase; Octanoyl-[acyl-carrier-protein]-protein N-octanoyltransferase

Number of amino acids: Translated: 233; Mature: 233

Protein sequence:

>233_residues
MFPAAGSPPVRWRISEGLVAYGQAVEEMEREVAAIAAGEADELVWLLEHPPLYTAGTSADVADLIEPDRFPVFATGRGGE
YTYHGPGQRVVYVMLDLKRRRQDVRAYVAALEDVIIRTLDKMNVRGERREDRVGVWVRRPEKPLLPDGGMAEDKIAALGI
RLRKWVSFHGLSINVDPDLSHFSGIVPCGISAYGVTSLVDLGLPVMIGDVDVLLREAFEEVFGPAVPETGAGG

Sequences:

>Translated_233_residues
MFPAAGSPPVRWRISEGLVAYGQAVEEMEREVAAIAAGEADELVWLLEHPPLYTAGTSADVADLIEPDRFPVFATGRGGE
YTYHGPGQRVVYVMLDLKRRRQDVRAYVAALEDVIIRTLDKMNVRGERREDRVGVWVRRPEKPLLPDGGMAEDKIAALGI
RLRKWVSFHGLSINVDPDLSHFSGIVPCGISAYGVTSLVDLGLPVMIGDVDVLLREAFEEVFGPAVPETGAGG
>Mature_233_residues
MFPAAGSPPVRWRISEGLVAYGQAVEEMEREVAAIAAGEADELVWLLEHPPLYTAGTSADVADLIEPDRFPVFATGRGGE
YTYHGPGQRVVYVMLDLKRRRQDVRAYVAALEDVIIRTLDKMNVRGERREDRVGVWVRRPEKPLLPDGGMAEDKIAALGI
RLRKWVSFHGLSINVDPDLSHFSGIVPCGISAYGVTSLVDLGLPVMIGDVDVLLREAFEEVFGPAVPETGAGG

Specific function: Catalyzes the transfer of endogenously produced octanoic acid from octanoyl-acyl-carrier-protein onto the lipoyl domains of lipoate-dependent enzymes. Lipoyl-ACP can also act as a substrate although octanoyl-ACP is likely to be the physiological substrate

COG id: COG0321

COG function: function code H; Lipoate-protein ligase B

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lipB family

Homologues:

Organism=Homo sapiens, GI221554520, Length=204, Percent_Identity=32.843137254902, Blast_Score=78, Evalue=7e-15,
Organism=Escherichia coli, GI87081767, Length=146, Percent_Identity=42.4657534246575, Blast_Score=128, Evalue=4e-31,
Organism=Saccharomyces cerevisiae, GI6323268, Length=200, Percent_Identity=28, Blast_Score=84, Evalue=2e-17,
Organism=Drosophila melanogaster, GI21355483, Length=185, Percent_Identity=34.0540540540541, Blast_Score=98, Evalue=5e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): LIPB_AGRT5 (Q8UF44)

Other databases:

- EMBL:   AE007869
- PIR:   AB2768
- PIR:   D97548
- RefSeq:   NP_354556.2
- ProteinModelPortal:   Q8UF44
- SMR:   Q8UF44
- STRING:   Q8UF44
- GeneID:   1133594
- GenomeReviews:   AE007869_GR
- KEGG:   atu:Atu1556
- eggNOG:   COG0321
- HOGENOM:   HBG716160
- OMA:   DLKRRRP
- PhylomeDB:   Q8UF44
- ProtClustDB:   PRK14341
- BioCyc:   ATUM176299-1:ATU1556-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00013
- InterPro:   IPR004143
- InterPro:   IPR000544
- InterPro:   IPR020605
- PIRSF:   PIRSF016262
- TIGRFAMs:   TIGR00214

Pfam domain/function: PF03099 BPL_LipA_LipB

EC number: =2.3.1.181

Molecular weight: Translated: 25450; Mature: 25450

Theoretical pI: Translated: 4.70; Mature: 4.70

Prosite motif: PS01313 LIPB

Important sites: ACT_SITE 223-223

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFPAAGSPPVRWRISEGLVAYGQAVEEMEREVAAIAAGEADELVWLLEHPPLYTAGTSAD
CCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHCCCHHHEEEEECCCCEEECCCCCC
VADLIEPDRFPVFATGRGGEYTYHGPGQRVVYVMLDLKRRRQDVRAYVAALEDVIIRTLD
HHHHCCCCCCEEEEECCCCCEEEECCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHH
KMNVRGERREDRVGVWVRRPEKPLLPDGGMAEDKIAALGIRLRKWVSFHGLSINVDPDLS
HCCCCCCCCCCCEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCEEEEECCCHH
HFSGIVPCGISAYGVTSLVDLGLPVMIGDVDVLLREAFEEVFGPAVPETGAGG
HHCCCCCCCCCHHHHHHHHHCCCCEEECHHHHHHHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure
MFPAAGSPPVRWRISEGLVAYGQAVEEMEREVAAIAAGEADELVWLLEHPPLYTAGTSAD
CCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHCCCHHHEEEEECCCCEEECCCCCC
VADLIEPDRFPVFATGRGGEYTYHGPGQRVVYVMLDLKRRRQDVRAYVAALEDVIIRTLD
HHHHCCCCCCEEEEECCCCCEEEECCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHH
KMNVRGERREDRVGVWVRRPEKPLLPDGGMAEDKIAALGIRLRKWVSFHGLSINVDPDLS
HCCCCCCCCCCCEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCEEEEECCCHH
HFSGIVPCGISAYGVTSLVDLGLPVMIGDVDVLLREAFEEVFGPAVPETGAGG
HHCCCCCCCCCHHHHHHHHHCCCCEEECHHHHHHHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11743193; 11743194