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The map label for this gene is dac [H]

Identifier: 159184785

GI number: 159184785

Start: 1488694

End: 1489866

Strand: Reverse

Name: dac [H]

Synonym: Atu1499

Alternate gene names: 159184785

Gene position: 1489866-1488694 (Counterclockwise)

Preceding gene: 159184786

Following gene: 159184784

Centisome position: 52.43

GC content: 59.34

Gene sequence:

>1173_bases
ATGCGCAAGGCCATACATCGCTCTTACCGGTCGGCCATCGTTGTGGCCGCCTTCGCATTTTTCAGTGCGGGTGCGGCGCA
ATCGCAGACAGCCCCTTTCATCGCCAAGGCTGAACAAGCCTATATGATCGATGCCGAAACCGGCACGGTGCTTCTTTCGC
AAAATGAAGATCAGTCCTTTCCGCCAGCATCGCTTGCCAAGCTGATGACGGTGGAAGTGGTGCTTGATGCGCTCTCCAAA
GGGCAGGTGACGCCGGAAACGGCCTATCCGGTCTCGGAATATGCCTGGCGCACGGGCGGCGCTCCCTCGCGCACCTCCAC
GATGTTTGCGGCCCTGAAATCCTCAGTCGGCATCAACGATCTGCTGACCGGCATCATCGTGCAGAATGCCAATGACGGCT
GCATCATCGTGGCTGAAGGCATGGCCGGTTCGGATGCCGCTTTCGCCAAGCGCATGACAGCGCGGGCGGGCGAACTTGGC
ATGAATCGCAGCATATTTTCCAATTCCACGGGACTTCCCGATCCCGGCAACCAGACGACCGCCAGGGATATGGTGCGTCT
GGCCCAGCATCTGCACGACACCTATCCCGATCGCTACGCGCTTTTCACCAAGCCCGATTTCGAGTGGAACCGGATTTTCC
AGCGCAACAAGAACACGCTGCTGATGCCGGGCAGCGGCATAGATGGTCTCGGGCTCGGTTTTGCGGAGGGCAGCGGTTTT
GCAGCTGTCGTTTCAGCCGAACGGGAAGGGCGCCGCGTCTATCTGGCGCTTGCAGGCATTGCCGATGACAAGACGCGACA
GGAAGAAGCCCGCCGGGTGGTGGACTGGGGCCTGACGGCTTTCGAAAAACGCCATCTTTTCAGCAAGGATGAAGCCGTCG
GCGCCGTCAGCGTTTATGGCGGCGATGTCTCGCATATCGATCTTTCACCGAAGGAAGATGTCAGCGTGCTGGTGCCGGTC
AATAATCCGGATCGCATTTCCGGCCGCATCGTCTATCGCTGGCCGCTGAATGCGCCGCTGGATGTCGGCGCGAACGCTGG
AACACTCAAGATATTTTCGGGCGAACGGCTGCTGCGTGAAGTGCCGCTTTATACGAAGACGGCGGTGGGCAAAGGTTCGC
TCACACAGAATGCGACGGGCGCGCTGAAGGAATTGCTGCTCTTCTGGCTGTGA

Upstream 100 bases:

>100_bases
AAGCGTCAGCAGGCGAAGTCTCGCTGACGAATTGAGAGTAAGGCTGCCTTTCGCTTTCATGGCGTGCAGCCGGTTGCAGC
ACGCAGGACTGGGCGGTTTC

Downstream 100 bases:

>100_bases
CGGCCATTTTCGCTGCCTTATTCCTATGCTTTTGTTCAGGCACTGGTTTTTCTCAAAAGCGCTTCACACATTTCGCTTCG
ATGCTGTAAACAGGCATTTC

Product: penicillin binding protein

Products: NA

Alternate protein names: DD-carboxypeptidase; DD-peptidase; Penicillin-binding protein 6B; PBP-6B [H]

Number of amino acids: Translated: 390; Mature: 390

Protein sequence:

>390_residues
MRKAIHRSYRSAIVVAAFAFFSAGAAQSQTAPFIAKAEQAYMIDAETGTVLLSQNEDQSFPPASLAKLMTVEVVLDALSK
GQVTPETAYPVSEYAWRTGGAPSRTSTMFAALKSSVGINDLLTGIIVQNANDGCIIVAEGMAGSDAAFAKRMTARAGELG
MNRSIFSNSTGLPDPGNQTTARDMVRLAQHLHDTYPDRYALFTKPDFEWNRIFQRNKNTLLMPGSGIDGLGLGFAEGSGF
AAVVSAEREGRRVYLALAGIADDKTRQEEARRVVDWGLTAFEKRHLFSKDEAVGAVSVYGGDVSHIDLSPKEDVSVLVPV
NNPDRISGRIVYRWPLNAPLDVGANAGTLKIFSGERLLREVPLYTKTAVGKGSLTQNATGALKELLLFWL

Sequences:

>Translated_390_residues
MRKAIHRSYRSAIVVAAFAFFSAGAAQSQTAPFIAKAEQAYMIDAETGTVLLSQNEDQSFPPASLAKLMTVEVVLDALSK
GQVTPETAYPVSEYAWRTGGAPSRTSTMFAALKSSVGINDLLTGIIVQNANDGCIIVAEGMAGSDAAFAKRMTARAGELG
MNRSIFSNSTGLPDPGNQTTARDMVRLAQHLHDTYPDRYALFTKPDFEWNRIFQRNKNTLLMPGSGIDGLGLGFAEGSGF
AAVVSAEREGRRVYLALAGIADDKTRQEEARRVVDWGLTAFEKRHLFSKDEAVGAVSVYGGDVSHIDLSPKEDVSVLVPV
NNPDRISGRIVYRWPLNAPLDVGANAGTLKIFSGERLLREVPLYTKTAVGKGSLTQNATGALKELLLFWL
>Mature_390_residues
MRKAIHRSYRSAIVVAAFAFFSAGAAQSQTAPFIAKAEQAYMIDAETGTVLLSQNEDQSFPPASLAKLMTVEVVLDALSK
GQVTPETAYPVSEYAWRTGGAPSRTSTMFAALKSSVGINDLLTGIIVQNANDGCIIVAEGMAGSDAAFAKRMTARAGELG
MNRSIFSNSTGLPDPGNQTTARDMVRLAQHLHDTYPDRYALFTKPDFEWNRIFQRNKNTLLMPGSGIDGLGLGFAEGSGF
AAVVSAEREGRRVYLALAGIADDKTRQEEARRVVDWGLTAFEKRHLFSKDEAVGAVSVYGGDVSHIDLSPKEDVSVLVPV
NNPDRISGRIVYRWPLNAPLDVGANAGTLKIFSGERLLREVPLYTKTAVGKGSLTQNATGALKELLLFWL

Specific function: Removes C-terminal D-alanyl residues from sugar-peptide cell wall precursors [H]

COG id: COG1686

COG function: function code M; D-alanyl-D-alanine carboxypeptidase

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein (Potential). Note=N-terminal lies in the periplasmic space (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S11 family [H]

Homologues:

Organism=Escherichia coli, GI87082022, Length=373, Percent_Identity=28.686327077748, Blast_Score=169, Evalue=2e-43,
Organism=Escherichia coli, GI1787062, Length=366, Percent_Identity=31.6939890710383, Blast_Score=165, Evalue=4e-42,
Organism=Escherichia coli, GI1786851, Length=357, Percent_Identity=28.2913165266106, Blast_Score=148, Evalue=8e-37,
Organism=Escherichia coli, GI87082054, Length=247, Percent_Identity=27.9352226720648, Blast_Score=64, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR015956
- InterPro:   IPR018044
- InterPro:   IPR012907
- InterPro:   IPR001967 [H]

Pfam domain/function: PF07943 PBP5_C; PF00768 Peptidase_S11 [H]

EC number: =3.4.16.4 [H]

Molecular weight: Translated: 42067; Mature: 42067

Theoretical pI: Translated: 7.70; Mature: 7.70

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRKAIHRSYRSAIVVAAFAFFSAGAAQSQTAPFIAKAEQAYMIDAETGTVLLSQNEDQSF
CCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEECCCEEEEECCCCEEEEECCCCCCC
PPASLAKLMTVEVVLDALSKGQVTPETAYPVSEYAWRTGGAPSRTSTMFAALKSSVGIND
CHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHCCCCCCCCHHHHHHHHHHHCCCHHH
LLTGIIVQNANDGCIIVAEGMAGSDAAFAKRMTARAGELGMNRSIFSNSTGLPDPGNQTT
HHHHHEEECCCCCEEEEECCCCCCHHHHHHHHHHHHHHCCCCCHHHCCCCCCCCCCCCHH
ARDMVRLAQHLHDTYPDRYALFTKPDFEWNRIFQRNKNTLLMPGSGIDGLGLGFAEGSGF
HHHHHHHHHHHHHCCCCCEEEEECCCCHHHHHHHCCCCEEEECCCCCCCCCCEECCCCCE
AAVVSAEREGRRVYLALAGIADDKTRQEEARRVVDWGLTAFEKRHLFSKDEAVGAVSVYG
EEEEECCCCCCEEEEEEECCCCCHHHHHHHHHHHHHCHHHHHHHHCCCCCCCCEEEEEEC
GDVSHIDLSPKEDVSVLVPVNNPDRISGRIVYRWPLNAPLDVGANAGTLKIFSGERLLRE
CCEEEECCCCCCCCEEEEECCCCCCCCCEEEEEECCCCCCCCCCCCCEEEEECCHHHHHH
VPLYTKTAVGKGSLTQNATGALKELLLFWL
CCCHHEECCCCCCCCCCHHHHHHHHHHHCC
>Mature Secondary Structure
MRKAIHRSYRSAIVVAAFAFFSAGAAQSQTAPFIAKAEQAYMIDAETGTVLLSQNEDQSF
CCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEECCCEEEEECCCCEEEEECCCCCCC
PPASLAKLMTVEVVLDALSKGQVTPETAYPVSEYAWRTGGAPSRTSTMFAALKSSVGIND
CHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHCCCCCCCCHHHHHHHHHHHCCCHHH
LLTGIIVQNANDGCIIVAEGMAGSDAAFAKRMTARAGELGMNRSIFSNSTGLPDPGNQTT
HHHHHEEECCCCCEEEEECCCCCCHHHHHHHHHHHHHHCCCCCHHHCCCCCCCCCCCCHH
ARDMVRLAQHLHDTYPDRYALFTKPDFEWNRIFQRNKNTLLMPGSGIDGLGLGFAEGSGF
HHHHHHHHHHHHHCCCCCEEEEECCCCHHHHHHHCCCCEEEECCCCCCCCCCEECCCCCE
AAVVSAEREGRRVYLALAGIADDKTRQEEARRVVDWGLTAFEKRHLFSKDEAVGAVSVYG
EEEEECCCCCCEEEEEEECCCCCHHHHHHHHHHHHHCHHHHHHHHCCCCCCCCEEEEEEC
GDVSHIDLSPKEDVSVLVPVNNPDRISGRIVYRWPLNAPLDVGANAGTLKIFSGERLLRE
CCEEEECCCCCCCCEEEEECCCCCCCCCEEEEEECCCCCCCCCCCCCEEEEECCHHHHHH
VPLYTKTAVGKGSLTQNATGALKELLLFWL
CCCHHEECCCCCCCCCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7737516; 11677609; 7751291 [H]