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The map label for this gene is aceF [H]

Identifier: 159184758

GI number: 159184758

Start: 1426572

End: 1427789

Strand: Direct

Name: aceF [H]

Synonym: Atu1432

Alternate gene names: 159184758

Gene position: 1426572-1427789 (Clockwise)

Preceding gene: 15888756

Following gene: 15888758

Centisome position: 50.2

GC content: 62.81

Gene sequence:

>1218_bases
GTGGAAGCCGTCGATGAAGGCACCGTCGCCAAGCTGGTTGTTCCGGCCGGCACCGAAGCCGTCAAGGTCAACGCCCTGAT
CGCGATCCTCGCAGCAGAAGGAGAAGATGTGGCCGAGGCGGCGAAGGGCGGCGATGCCGTTCCTGCCAAGGCGGAAGCTC
CCAAGCCGGAAGCCGCAAAGGCTGAAGCGCCGAAGGAAGAAGCTGCTCCAGTCAAGGCTGAAAAGCCGGTAGCCGATCAG
GCGGCCGCATCTTCCACACCGGCCCCGGTTGCCAAGTCCGGCGAGCGCATTTTCGCATCGCCACTTGCCCGCCGTCTTGC
CAAGGAAGCCGGTCTCGACCTTTCGGCCGTCTCCGGTTCCGGCCCGCATGGCCGTATCGTCAAGACCGATGTGGAAAAGG
CTGCCGCTTCCGGCGGTGCAAAGGCTGCTCCCGCCGCTGCTGCATCCGCAGGCGCACCGGCCCCGGCACTCGCCAAGGGC
CAGTCCGACGAAGCCGTTCTCAAGCTGTTCGAACAGGGCTCCTACGAGCTTGTGCCGCATGACGGCATGCGCAAGGTCAT
CGCCAAGCGTCTGGTCGAATCCAAGCAGACGGTTCCGCATTTCTACGTTTCCGTGGATTGCGAACTGGATACGCTTCTGG
CGCTGCGCGCCCAGCTCAACGCCGCTGCTCCCGAAAAGGACGGCAAGCCGGTCTACAAGCTGTCGGTCAACGACATGGTC
ATCAAGGCGCTGGCGCTGGCGCTGCGTGACGTTCCTGACGCCAACGTCTCCTGGACGGAAAGCGCCATGGTCAAGCACAA
GCACGCGGATGTCGGCGTTGCCGTTTCCATTCCGGGTGGCCTGATCACGCCGATCATCCGCAAAGCGGAAGAAAAGTCGC
TCTCCACCATCTCCAACGAGATGAAGGACTACGGCAAGCGCGCGAAAGAGCGCAAGCTGAAGCCCGAGGAATATCAGGGC
GGTACGACTGCCGTGTCCAACATGGGCATGATGGGCGTGAAAAGCTTCTCCGCCGTCATCAACCCGCCGCACGCCACCAT
TCTGGCTGTCGGTGCGGGCGAACAGCGTGCCGTCGTGAAGAACGGCGAGATCAAGATCGCCAACGTCATGACGGTCACGC
TTTCCACCGACCATCGCTGCGTCGATGGCGCGCTCGGAGCCGAACTGATCGGCGCGTTCAAGCGCTACATCGAAAACCCG
ATGGGCATGCTGGTTTGA

Upstream 100 bases:

>100_bases
GGAAGAGGGCAATCTGACCAAGTGGCTGGTCAAGGAAGGCGACAAGGTCGCCCCCGGTGACGTGATCGCCGAGATCGAGA
CCGACAAGGCGACCATGGAA

Downstream 100 bases:

>100_bases
TCGGAGAATAATATGGTGAAGTCGGTCCTCTGCTTTGGCGATTCTCTCACATGGGGATCAAATGCGGAAACGGGTGGCCG
GCACAGCCATGACGATCTTT

Product: branched-chain alpha-keto acid dehydrogenase subunit E2

Products: NA

Alternate protein names: Dihydrolipoamide acetyltransferase component of pyruvate dehydrogenase complex; E2 [H]

Number of amino acids: Translated: 405; Mature: 405

Protein sequence:

>405_residues
MEAVDEGTVAKLVVPAGTEAVKVNALIAILAAEGEDVAEAAKGGDAVPAKAEAPKPEAAKAEAPKEEAAPVKAEKPVADQ
AAASSTPAPVAKSGERIFASPLARRLAKEAGLDLSAVSGSGPHGRIVKTDVEKAAASGGAKAAPAAAASAGAPAPALAKG
QSDEAVLKLFEQGSYELVPHDGMRKVIAKRLVESKQTVPHFYVSVDCELDTLLALRAQLNAAAPEKDGKPVYKLSVNDMV
IKALALALRDVPDANVSWTESAMVKHKHADVGVAVSIPGGLITPIIRKAEEKSLSTISNEMKDYGKRAKERKLKPEEYQG
GTTAVSNMGMMGVKSFSAVINPPHATILAVGAGEQRAVVKNGEIKIANVMTVTLSTDHRCVDGALGAELIGAFKRYIENP
MGMLV

Sequences:

>Translated_405_residues
MEAVDEGTVAKLVVPAGTEAVKVNALIAILAAEGEDVAEAAKGGDAVPAKAEAPKPEAAKAEAPKEEAAPVKAEKPVADQ
AAASSTPAPVAKSGERIFASPLARRLAKEAGLDLSAVSGSGPHGRIVKTDVEKAAASGGAKAAPAAAASAGAPAPALAKG
QSDEAVLKLFEQGSYELVPHDGMRKVIAKRLVESKQTVPHFYVSVDCELDTLLALRAQLNAAAPEKDGKPVYKLSVNDMV
IKALALALRDVPDANVSWTESAMVKHKHADVGVAVSIPGGLITPIIRKAEEKSLSTISNEMKDYGKRAKERKLKPEEYQG
GTTAVSNMGMMGVKSFSAVINPPHATILAVGAGEQRAVVKNGEIKIANVMTVTLSTDHRCVDGALGAELIGAFKRYIENP
MGMLV
>Mature_405_residues
MEAVDEGTVAKLVVPAGTEAVKVNALIAILAAEGEDVAEAAKGGDAVPAKAEAPKPEAAKAEAPKEEAAPVKAEKPVADQ
AAASSTPAPVAKSGERIFASPLARRLAKEAGLDLSAVSGSGPHGRIVKTDVEKAAASGGAKAAPAAAASAGAPAPALAKG
QSDEAVLKLFEQGSYELVPHDGMRKVIAKRLVESKQTVPHFYVSVDCELDTLLALRAQLNAAAPEKDGKPVYKLSVNDMV
IKALALALRDVPDANVSWTESAMVKHKHADVGVAVSIPGGLITPIIRKAEEKSLSTISNEMKDYGKRAKERKLKPEEYQG
GTTAVSNMGMMGVKSFSAVINPPHATILAVGAGEQRAVVKNGEIKIANVMTVTLSTDHRCVDGALGAELIGAFKRYIENP
MGMLV

Specific function: The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2). It contains multiple copies of three enzymatic components:pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydroge

COG id: COG0508

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide acyltransferase (E2) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 lipoyl-binding domain [H]

Homologues:

Organism=Homo sapiens, GI31711992, Length=410, Percent_Identity=39.7560975609756, Blast_Score=285, Evalue=8e-77,
Organism=Homo sapiens, GI203098816, Length=432, Percent_Identity=33.7962962962963, Blast_Score=212, Evalue=6e-55,
Organism=Homo sapiens, GI203098753, Length=432, Percent_Identity=34.0277777777778, Blast_Score=212, Evalue=6e-55,
Organism=Homo sapiens, GI260898739, Length=160, Percent_Identity=41.875, Blast_Score=130, Evalue=2e-30,
Organism=Homo sapiens, GI110671329, Length=325, Percent_Identity=29.2307692307692, Blast_Score=114, Evalue=2e-25,
Organism=Homo sapiens, GI19923748, Length=225, Percent_Identity=29.7777777777778, Blast_Score=111, Evalue=1e-24,
Organism=Escherichia coli, GI1786946, Length=306, Percent_Identity=31.0457516339869, Blast_Score=144, Evalue=1e-35,
Organism=Escherichia coli, GI1786305, Length=302, Percent_Identity=30.794701986755, Blast_Score=137, Evalue=1e-33,
Organism=Caenorhabditis elegans, GI17560088, Length=414, Percent_Identity=41.0628019323672, Blast_Score=270, Evalue=9e-73,
Organism=Caenorhabditis elegans, GI17538894, Length=307, Percent_Identity=35.5048859934853, Blast_Score=172, Evalue=2e-43,
Organism=Caenorhabditis elegans, GI17537937, Length=347, Percent_Identity=32.2766570605187, Blast_Score=141, Evalue=5e-34,
Organism=Caenorhabditis elegans, GI25146366, Length=223, Percent_Identity=30.0448430493274, Blast_Score=118, Evalue=5e-27,
Organism=Saccharomyces cerevisiae, GI6324258, Length=414, Percent_Identity=40.3381642512077, Blast_Score=265, Evalue=1e-71,
Organism=Saccharomyces cerevisiae, GI6320352, Length=228, Percent_Identity=31.5789473684211, Blast_Score=120, Evalue=3e-28,
Organism=Drosophila melanogaster, GI20129315, Length=411, Percent_Identity=39.4160583941606, Blast_Score=229, Evalue=4e-60,
Organism=Drosophila melanogaster, GI24582497, Length=411, Percent_Identity=39.4160583941606, Blast_Score=228, Evalue=5e-60,
Organism=Drosophila melanogaster, GI18859875, Length=347, Percent_Identity=31.9884726224784, Blast_Score=133, Evalue=3e-31,
Organism=Drosophila melanogaster, GI24645909, Length=227, Percent_Identity=29.5154185022026, Blast_Score=103, Evalue=3e-22,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 3096 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003016
- InterPro:   IPR001078
- InterPro:   IPR006257
- InterPro:   IPR000089
- InterPro:   IPR023213
- InterPro:   IPR004167
- InterPro:   IPR011053 [H]

Pfam domain/function: PF00198 2-oxoacid_dh; PF00364 Biotin_lipoyl; PF02817 E3_binding [H]

EC number: =2.3.1.12 [H]

Molecular weight: Translated: 41861; Mature: 41861

Theoretical pI: Translated: 7.69; Mature: 7.69

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEAVDEGTVAKLVVPAGTEAVKVNALIAILAAEGEDVAEAAKGGDAVPAKAEAPKPEAAK
CCCCCCCCEEEEEECCCCCEEEHEEEEEEEECCCCHHHHHHCCCCCCCCCCCCCCCCCCC
AEAPKEEAAPVKAEKPVADQAAASSTPAPVAKSGERIFASPLARRLAKEAGLDLSAVSGS
CCCCHHHCCCCCCCCCCHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCEEEECCC
GPHGRIVKTDVEKAAASGGAKAAPAAAASAGAPAPALAKGQSDEAVLKLFEQGSYELVPH
CCCCCEEECHHHHHHHCCCCCCCCCHHHCCCCCCCHHHCCCCHHHHHHHHHCCCEEECCC
DGMRKVIAKRLVESKQTVPHFYVSVDCELDTLLALRAQLNAAAPEKDGKPVYKLSVNDMV
HHHHHHHHHHHHHHHCCCCEEEEEEECCHHHHHHHHHHHCCCCCCCCCCCEEEEEHHHHH
IKALALALRDVPDANVSWTESAMVKHKHADVGVAVSIPGGLITPIIRKAEEKSLSTISNE
HHHHHHHHHCCCCCCCCCCHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHH
MKDYGKRAKERKLKPEEYQGGTTAVSNMGMMGVKSFSAVINPPHATILAVGAGEQRAVVK
HHHHHHHHHHHCCCCHHHCCCHHHHHHCCHHHHHHHHHHCCCCCEEEEEECCCCCEEEEE
NGEIKIANVMTVTLSTDHRCVDGALGAELIGAFKRYIENPMGMLV
CCCEEEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHCCCCHHCC
>Mature Secondary Structure
MEAVDEGTVAKLVVPAGTEAVKVNALIAILAAEGEDVAEAAKGGDAVPAKAEAPKPEAAK
CCCCCCCCEEEEEECCCCCEEEHEEEEEEEECCCCHHHHHHCCCCCCCCCCCCCCCCCCC
AEAPKEEAAPVKAEKPVADQAAASSTPAPVAKSGERIFASPLARRLAKEAGLDLSAVSGS
CCCCHHHCCCCCCCCCCHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCEEEECCC
GPHGRIVKTDVEKAAASGGAKAAPAAAASAGAPAPALAKGQSDEAVLKLFEQGSYELVPH
CCCCCEEECHHHHHHHCCCCCCCCCHHHCCCCCCCHHHCCCCHHHHHHHHHCCCEEECCC
DGMRKVIAKRLVESKQTVPHFYVSVDCELDTLLALRAQLNAAAPEKDGKPVYKLSVNDMV
HHHHHHHHHHHHHHHCCCCEEEEEEECCHHHHHHHHHHHCCCCCCCCCCCEEEEEHHHHH
IKALALALRDVPDANVSWTESAMVKHKHADVGVAVSIPGGLITPIIRKAEEKSLSTISNE
HHHHHHHHHCCCCCCCCCCHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHH
MKDYGKRAKERKLKPEEYQGGTTAVSNMGMMGVKSFSAVINPPHATILAVGAGEQRAVVK
HHHHHHHHHHHCCCCHHHCCCHHHHHHCCHHHHHHHHHHCCCCCEEEEEECCCCCEEEEE
NGEIKIANVMTVTLSTDHRCVDGALGAELIGAFKRYIENPMGMLV
CCCEEEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHCCCCHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10796014; 11481430 [H]