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The map label for this gene is gutB [H]

Identifier: 159184747

GI number: 159184747

Start: 1402611

End: 1403654

Strand: Direct

Name: gutB [H]

Synonym: Atu1408

Alternate gene names: 159184747

Gene position: 1402611-1403654 (Clockwise)

Preceding gene: 15888733

Following gene: 15888735

Centisome position: 49.36

GC content: 61.88

Gene sequence:

>1044_bases
ATGCGCGGAGTCGTCATTCATGCAGCAAAAGACCTGCGGGTAGAGGACGTTGCTGGCCAGCCACTTGCCGCGGACGAGGT
GCGGGTGGCCGTTGCCGTCGGCGGAATTTGCGGCTCGGATCTGCATTATTATAACCATGGCGGCTTCGGCACGGTGCGCG
TGCGCGAGCCGATGGCGCTCGGTCATGAGTTTGCCGGTACGGTGGTTGAGGTGGGCAGTTCGGTCTCGCATCTCGTGCCC
GGCATGCGCGTGGCCGTCAATCCGAGCCTGCCTTGCGGCACCTGCCGCTATTGCGCTCAGGGCAGGCAGAATCAGTGCCT
GGACATGCGCTTCATGGGCAGCGCCATGCGCTCCCCCCATGTTCAGGGCGGTTTCCGTGAAGTCGTGACCGTCCATTCAA
CGCAACCGGTACAGATCGCCGACGGACTTTCCATGGGTGAGGCAGCCATGGCCGAGCCTTTGGCCGTGTGCCTCCATGCC
GCGCGTCAGGCGGGATCGCTTCTGGGCAAGACGGTGCTGATAACCGGTGCCGGGCCGATCGGCATGCTTAGCCTGCTGGT
TGCCCGTCTTGCCGGCGCGGCGCATATCGTCGTTACCGATGTCGCCGATGCACCGCTCGATCTGGCGCGACGTATCGGCG
CGGATGAAGCCGTCAACATCCTGCGCGATGCCGACATGCTTGAAAAATACCGATTTGAAAAAGGCGTCTTCGACGTCCTG
TTCGAAGCCTCCGGCAATCAGGCGGCACTTCTCCCGGCGCTGGATCTGCTCCGGCCGGGCGGTATTATCGTCCAGCTCGG
TCTTGGCGGAGACTTCACCATTCCGATGAACCTCATCGTTGCCAAAGAGCTGCAGCTGCGCGGAACGTTCCGCTTCCACG
AGGAATTTGCCCAGGCGGTGAATATGATGGGACGTGGCCTGATCGACGTTAAGCCTTTGATCAGCGCCACATTGCCGTTC
GATCAGGCCCGCGAGGCTTTCGATCTTGCCGGTGACCGCGCAAAAAGCATGAAAGTGCAGCTTGCCTTCAGCGGAGCAGC
CTGA

Upstream 100 bases:

>100_bases
GTCAATGGCCAGATAATCTATGTCGATGGTGGAATGCTGTCGGTTCTCTAGAACCGCCGCCATCCTTGATGCCAACAGAA
ATTGGCGGGAGGAGACGCGC

Downstream 100 bases:

>100_bases
TGAAGACCGTTACCGCCAGGCCGCTGACAGCGGAAGCCTTCGCGCCCTACGGCTCTGTCGCCGACATCTCCGAACTTGAA
AATCTGGTGTCGCTTGCGGA

Product: sorbitol dehydrogenase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 347; Mature: 347

Protein sequence:

>347_residues
MRGVVIHAAKDLRVEDVAGQPLAADEVRVAVAVGGICGSDLHYYNHGGFGTVRVREPMALGHEFAGTVVEVGSSVSHLVP
GMRVAVNPSLPCGTCRYCAQGRQNQCLDMRFMGSAMRSPHVQGGFREVVTVHSTQPVQIADGLSMGEAAMAEPLAVCLHA
ARQAGSLLGKTVLITGAGPIGMLSLLVARLAGAAHIVVTDVADAPLDLARRIGADEAVNILRDADMLEKYRFEKGVFDVL
FEASGNQAALLPALDLLRPGGIIVQLGLGGDFTIPMNLIVAKELQLRGTFRFHEEFAQAVNMMGRGLIDVKPLISATLPF
DQAREAFDLAGDRAKSMKVQLAFSGAA

Sequences:

>Translated_347_residues
MRGVVIHAAKDLRVEDVAGQPLAADEVRVAVAVGGICGSDLHYYNHGGFGTVRVREPMALGHEFAGTVVEVGSSVSHLVP
GMRVAVNPSLPCGTCRYCAQGRQNQCLDMRFMGSAMRSPHVQGGFREVVTVHSTQPVQIADGLSMGEAAMAEPLAVCLHA
ARQAGSLLGKTVLITGAGPIGMLSLLVARLAGAAHIVVTDVADAPLDLARRIGADEAVNILRDADMLEKYRFEKGVFDVL
FEASGNQAALLPALDLLRPGGIIVQLGLGGDFTIPMNLIVAKELQLRGTFRFHEEFAQAVNMMGRGLIDVKPLISATLPF
DQAREAFDLAGDRAKSMKVQLAFSGAA
>Mature_347_residues
MRGVVIHAAKDLRVEDVAGQPLAADEVRVAVAVGGICGSDLHYYNHGGFGTVRVREPMALGHEFAGTVVEVGSSVSHLVP
GMRVAVNPSLPCGTCRYCAQGRQNQCLDMRFMGSAMRSPHVQGGFREVVTVHSTQPVQIADGLSMGEAAMAEPLAVCLHA
ARQAGSLLGKTVLITGAGPIGMLSLLVARLAGAAHIVVTDVADAPLDLARRIGADEAVNILRDADMLEKYRFEKGVFDVL
FEASGNQAALLPALDLLRPGGIIVQLGLGGDFTIPMNLIVAKELQLRGTFRFHEEFAQAVNMMGRGLIDVKPLISATLPF
DQAREAFDLAGDRAKSMKVQLAFSGAA

Specific function: Catalyzes the NADH/NADPH-dependent oxidation of L- idonate to 5-ketogluconate (5KG) [H]

COG id: COG1063

COG function: function code ER; Threonine dehydrogenase and related Zn-dependent dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the zinc-containing alcohol dehydrogenase family [H]

Homologues:

Organism=Homo sapiens, GI156627571, Length=329, Percent_Identity=36.1702127659575, Blast_Score=209, Evalue=3e-54,
Organism=Homo sapiens, GI4501933, Length=374, Percent_Identity=27.0053475935829, Blast_Score=97, Evalue=2e-20,
Organism=Homo sapiens, GI4501929, Length=375, Percent_Identity=26.6666666666667, Blast_Score=94, Evalue=1e-19,
Organism=Homo sapiens, GI34577061, Length=377, Percent_Identity=25.4641909814324, Blast_Score=93, Evalue=3e-19,
Organism=Homo sapiens, GI71565154, Length=357, Percent_Identity=23.8095238095238, Blast_Score=79, Evalue=7e-15,
Organism=Homo sapiens, GI262073058, Length=375, Percent_Identity=23.4666666666667, Blast_Score=71, Evalue=2e-12,
Organism=Homo sapiens, GI71743840, Length=375, Percent_Identity=23.4666666666667, Blast_Score=71, Evalue=2e-12,
Organism=Escherichia coli, GI1790718, Length=316, Percent_Identity=46.8354430379747, Blast_Score=281, Evalue=6e-77,
Organism=Escherichia coli, GI1788073, Length=318, Percent_Identity=32.0754716981132, Blast_Score=169, Evalue=3e-43,
Organism=Escherichia coli, GI1790045, Length=344, Percent_Identity=30.8139534883721, Blast_Score=156, Evalue=2e-39,
Organism=Escherichia coli, GI1787863, Length=349, Percent_Identity=30.3724928366762, Blast_Score=143, Evalue=1e-35,
Organism=Escherichia coli, GI1788075, Length=348, Percent_Identity=30.1724137931034, Blast_Score=131, Evalue=7e-32,
Organism=Escherichia coli, GI1788407, Length=353, Percent_Identity=30.3116147308782, Blast_Score=128, Evalue=6e-31,
Organism=Escherichia coli, GI226510992, Length=314, Percent_Identity=31.2101910828025, Blast_Score=122, Evalue=4e-29,
Organism=Escherichia coli, GI1786825, Length=395, Percent_Identity=26.3291139240506, Blast_Score=99, Evalue=4e-22,
Organism=Escherichia coli, GI87081918, Length=236, Percent_Identity=32.2033898305085, Blast_Score=79, Evalue=3e-16,
Organism=Escherichia coli, GI87082125, Length=350, Percent_Identity=26.2857142857143, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI1786518, Length=237, Percent_Identity=29.1139240506329, Blast_Score=66, Evalue=3e-12,
Organism=Caenorhabditis elegans, GI17562876, Length=330, Percent_Identity=36.6666666666667, Blast_Score=220, Evalue=1e-57,
Organism=Caenorhabditis elegans, GI17562878, Length=328, Percent_Identity=36.280487804878, Blast_Score=192, Evalue=2e-49,
Organism=Caenorhabditis elegans, GI25146526, Length=383, Percent_Identity=24.8041775456919, Blast_Score=85, Evalue=6e-17,
Organism=Caenorhabditis elegans, GI17562584, Length=305, Percent_Identity=25.2459016393443, Blast_Score=76, Evalue=3e-14,
Organism=Caenorhabditis elegans, GI71988145, Length=326, Percent_Identity=25.1533742331288, Blast_Score=68, Evalue=6e-12,
Organism=Saccharomyces cerevisiae, GI6322619, Length=313, Percent_Identity=35.4632587859425, Blast_Score=186, Evalue=6e-48,
Organism=Saccharomyces cerevisiae, GI6319955, Length=313, Percent_Identity=35.1437699680511, Blast_Score=183, Evalue=3e-47,
Organism=Saccharomyces cerevisiae, GI6323099, Length=313, Percent_Identity=32.9073482428115, Blast_Score=169, Evalue=6e-43,
Organism=Saccharomyces cerevisiae, GI6319257, Length=343, Percent_Identity=30.9037900874636, Blast_Score=135, Evalue=1e-32,
Organism=Saccharomyces cerevisiae, GI6319258, Length=333, Percent_Identity=27.027027027027, Blast_Score=120, Evalue=4e-28,
Organism=Saccharomyces cerevisiae, GI6320033, Length=370, Percent_Identity=24.5945945945946, Blast_Score=93, Evalue=7e-20,
Organism=Saccharomyces cerevisiae, GI6323729, Length=315, Percent_Identity=25.0793650793651, Blast_Score=91, Evalue=3e-19,
Organism=Saccharomyces cerevisiae, GI6319621, Length=304, Percent_Identity=25.3289473684211, Blast_Score=79, Evalue=1e-15,
Organism=Saccharomyces cerevisiae, GI6324486, Length=328, Percent_Identity=23.780487804878, Blast_Score=75, Evalue=1e-14,
Organism=Saccharomyces cerevisiae, GI6323961, Length=304, Percent_Identity=23.3552631578947, Blast_Score=67, Evalue=5e-12,
Organism=Saccharomyces cerevisiae, GI6319949, Length=280, Percent_Identity=27.8571428571429, Blast_Score=65, Evalue=1e-11,
Organism=Drosophila melanogaster, GI17737897, Length=331, Percent_Identity=35.6495468277946, Blast_Score=194, Evalue=1e-49,
Organism=Drosophila melanogaster, GI17137530, Length=331, Percent_Identity=32.6283987915408, Blast_Score=179, Evalue=2e-45,
Organism=Drosophila melanogaster, GI17737895, Length=360, Percent_Identity=28.0555555555556, Blast_Score=96, Evalue=3e-20,
Organism=Drosophila melanogaster, GI221457811, Length=305, Percent_Identity=27.5409836065574, Blast_Score=96, Evalue=5e-20,
Organism=Drosophila melanogaster, GI45550770, Length=305, Percent_Identity=27.5409836065574, Blast_Score=96, Evalue=5e-20,
Organism=Drosophila melanogaster, GI45551930, Length=305, Percent_Identity=27.5409836065574, Blast_Score=96, Evalue=5e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013149
- InterPro:   IPR013154
- InterPro:   IPR002085
- InterPro:   IPR011032
- InterPro:   IPR016040 [H]

Pfam domain/function: PF08240 ADH_N; PF00107 ADH_zinc_N [H]

EC number: =1.1.1.264 [H]

Molecular weight: Translated: 36674; Mature: 36674

Theoretical pI: Translated: 6.67; Mature: 6.67

Prosite motif: PS00059 ADH_ZINC

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
5.8 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
5.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRGVVIHAAKDLRVEDVAGQPLAADEVRVAVAVGGICGSDLHYYNHGGFGTVRVREPMAL
CCCEEEEECCCCEEECCCCCCCCCCCEEEEEEECCCCCCCCCEEECCCEEEEEECCCHHH
GHEFAGTVVEVGSSVSHLVPGMRVAVNPSLPCGTCRYCAQGRQNQCLDMRFMGSAMRSPH
CHHHHHHHHHHCCCHHHHCCCCEEEECCCCCCCHHHHHHCCCCCCCHHHHHHHHHHCCCC
VQGGFREVVTVHSTQPVQIADGLSMGEAAMAEPLAVCLHAARQAGSLLGKTVLITGAGPI
CCCCCEEEEEECCCCCEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCHH
GMLSLLVARLAGAAHIVVTDVADAPLDLARRIGADEAVNILRDADMLEKYRFEKGVFDVL
HHHHHHHHHHCCCEEEEEEECCCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCEEHE
FEASGNQAALLPALDLLRPGGIIVQLGLGGDFTIPMNLIVAKELQLRGTFRFHEEFAQAV
EECCCCCEEEHHHHHHHCCCCEEEEEECCCCCEECCCEEEEEHHHHCCHHHHHHHHHHHH
NMMGRGLIDVKPLISATLPFDQAREAFDLAGDRAKSMKVQLAFSGAA
HHHCCCCCCHHHHHHHCCCHHHHHHHHHHCCCCCCCEEEEEEECCCC
>Mature Secondary Structure
MRGVVIHAAKDLRVEDVAGQPLAADEVRVAVAVGGICGSDLHYYNHGGFGTVRVREPMAL
CCCEEEEECCCCEEECCCCCCCCCCCEEEEEEECCCCCCCCCEEECCCEEEEEECCCHHH
GHEFAGTVVEVGSSVSHLVPGMRVAVNPSLPCGTCRYCAQGRQNQCLDMRFMGSAMRSPH
CHHHHHHHHHHCCCHHHHCCCCEEEECCCCCCCHHHHHHCCCCCCCHHHHHHHHHHCCCC
VQGGFREVVTVHSTQPVQIADGLSMGEAAMAEPLAVCLHAARQAGSLLGKTVLITGAGPI
CCCCCEEEEEECCCCCEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCHH
GMLSLLVARLAGAAHIVVTDVADAPLDLARRIGADEAVNILRDADMLEKYRFEKGVFDVL
HHHHHHHHHHCCCEEEEEEECCCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCEEHE
FEASGNQAALLPALDLLRPGGIIVQLGLGGDFTIPMNLIVAKELQLRGTFRFHEEFAQAV
EECCCCCEEEHHHHHHHCCCCEEEEEECCCCCEECCCEEEEEHHHHCCHHHHHHHHHHHH
NMMGRGLIDVKPLISATLPFDQAREAFDLAGDRAKSMKVQLAFSGAA
HHHCCCCCCHHHHHHHCCCHHHHHHHHHHCCCCCCCEEEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7610040; 9278503; 9658018 [H]