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The map label for this gene is amiA [H]

Identifier: 159184720

GI number: 159184720

Start: 1327380

End: 1328639

Strand: Direct

Name: amiA [H]

Synonym: Atu1340

Alternate gene names: 159184720

Gene position: 1327380-1328639 (Clockwise)

Preceding gene: 159184718

Following gene: 15888667

Centisome position: 46.71

GC content: 60.24

Gene sequence:

>1260_bases
ATGGATTTCACGCGCAGCGCAACGATATCAGGCGGGGCAGGGCGGCTTGGCGCGCGAATCGCGCGGCTTGCGTCGGCTTT
GGCATTTTTCGTCGCCACCTTAGTCTCCGTCGTGCCGACAGCCCATGCGGCGGAGCCGCTTGTCGCCCACCAGGCGCGGA
TCATCGGTGATGAGGCGCGCACGCGCATCGTTCTCGATTTTGCCGAAGAGCCGGAATTCGACGTTCACTATCTCGATTCG
CCCGCCCGCATCGTCGTCGACTTTCCCGCGGTGAATTTTGCGTTCCCCACGTCCGACCTCAAACCAACAGGCCTTTTTTC
GGATATACGCTTCGGCACCATGGGGGAGAACAGTGCCCGCATCGTGCTGACGGCGAAAAAGCCTGTTCAAGTGACGGTTG
CCGAAACCAAAAAGGGCGATGACGGCCAGTCTTTCCGTTTCGTACTCGATGCCGAGATCACCACCAAGGGCAAGTTTGCG
GAACTGGTGAAGGATCAGCAATGGCGCGCGCCAGCCCCGGTGACAACGGCCGCGATCACGCCCGATGACAAGACATCGCG
GCCAGCGGAATTCGTCATCGCGGTCGATGCCGGCCATGGCGGCATCGACGCAGGCGCCACTGGCGGCACGACGGGCACCG
AGGAAAAGGTCATCACCTTGACCTTTGCGAAGGAGCTTGTGGAGCGGTTGAACCGCGAAGCGGGCATCAAGGCCTTTTTA
ACGCGTGATTCCGACACCTTCCTCGCCTTGTCGGAACGGGTGACAATCGCCCGCCAGAACAATGCCAATCTCTTTATATC
CCTTCACGCCGACACGCTGAAGCAGAAGGGAATTCGCGGCGCCACCGTCTATACCCTGTCGGACCGGGCATCCGACCGGC
AGGCGCAGGAGTTGGCAGAGCGCGAAAACAAGTCAGACCAGATTGCCGGCGTTGCCGCTTCCACGGAGCCGCCGGAGGTC
GCCGATATTCTCCTCGATTTCACCCGCCGCGAGACCCAGGCCTTTTCGGTAACGCTGGCCGAAAACATCGTCTCTTCCTT
CGAGGGGCAGGTTGGCCTTATCAACAATCCGCACCGTTATGCCGGTTTCATGGTTCTCCGCGCCCATGATGTGCCGTCGG
TGCTTCTTGAGCTTGGGTTCCTTTCGAATGCGGAAGACGAAAAGCTGCTTCTCGATATCGAGTGGCGCAAAAAAGTAGCC
GACACATTGGCAACGGCCGTCGGGCGATACCGTTCCAAGGCCCTGGCGAATGGCGGCTGA

Upstream 100 bases:

>100_bases
GGCGTAAACTATCGTGCGGCGTGGATGAAACGGCACCATTGCCGGCACTTTTCCGCGCCGTTTATCGAAAATGACTGAAA
TACGGAATTTCTTGAGGGCC

Downstream 100 bases:

>100_bases
TAGCGGTGGAGACGCTGTTGCCGCCCTGCCATTGATCGTGGAAAAAGCTGTTCAATTCTAAGGCTTGCGGTCGATTAACG
ACCCGAAGCCCTATTTTACT

Product: N-acetylmuramoyl-L-alanine amidase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 419; Mature: 419

Protein sequence:

>419_residues
MDFTRSATISGGAGRLGARIARLASALAFFVATLVSVVPTAHAAEPLVAHQARIIGDEARTRIVLDFAEEPEFDVHYLDS
PARIVVDFPAVNFAFPTSDLKPTGLFSDIRFGTMGENSARIVLTAKKPVQVTVAETKKGDDGQSFRFVLDAEITTKGKFA
ELVKDQQWRAPAPVTTAAITPDDKTSRPAEFVIAVDAGHGGIDAGATGGTTGTEEKVITLTFAKELVERLNREAGIKAFL
TRDSDTFLALSERVTIARQNNANLFISLHADTLKQKGIRGATVYTLSDRASDRQAQELAERENKSDQIAGVAASTEPPEV
ADILLDFTRRETQAFSVTLAENIVSSFEGQVGLINNPHRYAGFMVLRAHDVPSVLLELGFLSNAEDEKLLLDIEWRKKVA
DTLATAVGRYRSKALANGG

Sequences:

>Translated_419_residues
MDFTRSATISGGAGRLGARIARLASALAFFVATLVSVVPTAHAAEPLVAHQARIIGDEARTRIVLDFAEEPEFDVHYLDS
PARIVVDFPAVNFAFPTSDLKPTGLFSDIRFGTMGENSARIVLTAKKPVQVTVAETKKGDDGQSFRFVLDAEITTKGKFA
ELVKDQQWRAPAPVTTAAITPDDKTSRPAEFVIAVDAGHGGIDAGATGGTTGTEEKVITLTFAKELVERLNREAGIKAFL
TRDSDTFLALSERVTIARQNNANLFISLHADTLKQKGIRGATVYTLSDRASDRQAQELAERENKSDQIAGVAASTEPPEV
ADILLDFTRRETQAFSVTLAENIVSSFEGQVGLINNPHRYAGFMVLRAHDVPSVLLELGFLSNAEDEKLLLDIEWRKKVA
DTLATAVGRYRSKALANGG
>Mature_419_residues
MDFTRSATISGGAGRLGARIARLASALAFFVATLVSVVPTAHAAEPLVAHQARIIGDEARTRIVLDFAEEPEFDVHYLDS
PARIVVDFPAVNFAFPTSDLKPTGLFSDIRFGTMGENSARIVLTAKKPVQVTVAETKKGDDGQSFRFVLDAEITTKGKFA
ELVKDQQWRAPAPVTTAAITPDDKTSRPAEFVIAVDAGHGGIDAGATGGTTGTEEKVITLTFAKELVERLNREAGIKAFL
TRDSDTFLALSERVTIARQNNANLFISLHADTLKQKGIRGATVYTLSDRASDRQAQELAERENKSDQIAGVAASTEPPEV
ADILLDFTRRETQAFSVTLAENIVSSFEGQVGLINNPHRYAGFMVLRAHDVPSVLLELGFLSNAEDEKLLLDIEWRKKVA
DTLATAVGRYRSKALANGG

Specific function: Cell-wall hydrolase involved in septum cleavage during cell division. Can also act as powerful autolysin in the presence of murein synthesis inhibitors [H]

COG id: COG0860

COG function: function code M; N-acetylmuramoyl-L-alanine amidase

Gene ontology:

Cell location: Periplasm. Note=Distributed throughout the periplasm in all cells [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N-acetylmuramoyl-L-alanine amidase 3 family [H]

Homologues:

Organism=Escherichia coli, GI1788776, Length=220, Percent_Identity=40, Blast_Score=149, Evalue=3e-37,
Organism=Escherichia coli, GI1790611, Length=250, Percent_Identity=33.2, Blast_Score=137, Evalue=1e-33,
Organism=Escherichia coli, GI87082163, Length=389, Percent_Identity=29.8200514138817, Blast_Score=135, Evalue=4e-33,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002508
- InterPro:   IPR006311 [H]

Pfam domain/function: PF01520 Amidase_3 [H]

EC number: =3.5.1.28 [H]

Molecular weight: Translated: 45241; Mature: 45241

Theoretical pI: Translated: 5.53; Mature: 5.53

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
0.7 %Met     (Translated Protein)
0.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
0.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDFTRSATISGGAGRLGARIARLASALAFFVATLVSVVPTAHAAEPLVAHQARIIGDEAR
CCCCCCEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHCCCCCC
TRIVLDFAEEPEFDVHYLDSPARIVVDFPAVNFAFPTSDLKPTGLFSDIRFGTMGENSAR
EEEEEEECCCCCCCEEEECCCCEEEEEECCCEEECCCCCCCCCCCHHCCCCCCCCCCCCE
IVLTAKKPVQVTVAETKKGDDGQSFRFVLDAEITTKGKFAELVKDQQWRAPAPVTTAAIT
EEEEECCCEEEEEEECCCCCCCCEEEEEEEEEECCCCHHHHHHCCCCCCCCCCEEEEEEC
PDDKTSRPAEFVIAVDAGHGGIDAGATGGTTGTEEKVITLTFAKELVERLNREAGIKAFL
CCCCCCCCEEEEEEEECCCCCCCCCCCCCCCCCCCEEEEEHHHHHHHHHHCHHCCCEEEE
TRDSDTFLALSERVTIARQNNANLFISLHADTLKQKGIRGATVYTLSDRASDRQAQELAE
ECCCCCEEEEECEEEEEEECCCEEEEEEEHHHHHHCCCCCEEEEEECCCCCHHHHHHHHH
RENKSDQIAGVAASTEPPEVADILLDFTRRETQAFSVTLAENIVSSFEGQVGLINNPHRY
HCCCCCCEEEEECCCCCHHHHHHHHHHHHCCCCEEEEHHHHHHHHHCCCCEEECCCCCCE
AGFMVLRAHDVPSVLLELGFLSNAEDEKLLLDIEWRKKVADTLATAVGRYRSKALANGG
EEEEEEEECCHHHHHHHHHCCCCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MDFTRSATISGGAGRLGARIARLASALAFFVATLVSVVPTAHAAEPLVAHQARIIGDEAR
CCCCCCEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHCCCCCC
TRIVLDFAEEPEFDVHYLDSPARIVVDFPAVNFAFPTSDLKPTGLFSDIRFGTMGENSAR
EEEEEEECCCCCCCEEEECCCCEEEEEECCCEEECCCCCCCCCCCHHCCCCCCCCCCCCE
IVLTAKKPVQVTVAETKKGDDGQSFRFVLDAEITTKGKFAELVKDQQWRAPAPVTTAAIT
EEEEECCCEEEEEEECCCCCCCCEEEEEEEEEECCCCHHHHHHCCCCCCCCCCEEEEEEC
PDDKTSRPAEFVIAVDAGHGGIDAGATGGTTGTEEKVITLTFAKELVERLNREAGIKAFL
CCCCCCCCEEEEEEEECCCCCCCCCCCCCCCCCCCEEEEEHHHHHHHHHHCHHCCCEEEE
TRDSDTFLALSERVTIARQNNANLFISLHADTLKQKGIRGATVYTLSDRASDRQAQELAE
ECCCCCEEEEECEEEEEEECCCEEEEEEEHHHHHHCCCCCEEEEEECCCCCHHHHHHHHH
RENKSDQIAGVAASTEPPEVADILLDFTRRETQAFSVTLAENIVSSFEGQVGLINNPHRY
HCCCCCCEEEEECCCCCHHHHHHHHHHHHCCCCEEEEHHHHHHHHHCCCCEEECCCCCCE
AGFMVLRAHDVPSVLLELGFLSNAEDEKLLLDIEWRKKVADTLATAVGRYRSKALANGG
EEEEEEEECCHHHHHHHHHCCCCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8300522; 9205837; 9278503; 1903834 [H]