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The map label for this gene is wcaJ [H]

Identifier: 159184679

GI number: 159184679

Start: 1224313

End: 1225866

Strand: Reverse

Name: wcaJ [H]

Synonym: Atu1236

Alternate gene names: 159184679

Gene position: 1225866-1224313 (Counterclockwise)

Preceding gene: 15888568

Following gene: 15888566

Centisome position: 43.14

GC content: 58.69

Gene sequence:

>1554_bases
ATGACCAAGACTGACAAGGACAGACTGCAATTCGATCTGGCGTCATTGCGCAAGAAACTCTCCGACTACGAAGCCGGGGG
TGCTGCGGCATCCGAAACCATCGTTCTCAATCCGCTTGCACGGCAAATTGCCAACCAGCTTCGCGCCGGTAACCATTCAC
CTAACATGGTGATCGGGCAATTGCGGCTTCTCGAATTCACGATGCTCGTGATCATAGCTGCCGTCGTCAACGGTATTGCA
GCCGATCGCGGCCTTGAAACCTTCCTGAAGGTTTTCAGCGGCGGCGTGCTGGGTGCGGCACTCTGCGTCTTCCTGCTGCA
GGCGGGTGATTGCTATCAGCTTCCCACGTTGCGCACGCCGCTAAAAATGTTTGCCCGCATTCAGGGCGCCGTCTGCCTTT
CCTTCGTGGGAATTGCCTGTTTCCTTCTCGTCTTCTTCCCGGAAACGCTGCAGTCCTGGCAGGCCTTCGCCATCTGGTAT
GCAACGGGCGCGATCGCGATTTTCGCCGGTCGGTTGATCCTTGGCTTTGCCATCCGCCACTGGGGCCGCAACGGCGTGAT
GGAACGCCGCGCCGTCATCGTCGGCGGCGGCGATGCCGCGAGAGACCTTATCCGTTCGCTGGAGCAGCAGTCCGACAACG
ACATCCGCATCTGCGGTATTTTCGACGACCGGAAAAGCGAGCGGTCTCCCGAAGTTGTCGCCGGTTATCCCAAGCTCGGC
ACCTTTGCGGAACTGGTGGAATTCGCCCGCCTGACGAAGCTCGACATGCTGATCATCGCCCTGCCGCTCAGCGCCGAGGC
GCGCATTCTCCAGCTTCTGCGCAAGCTGTGGGTTTTGCCGGTCGATATCCGCATTGCCGCCCATGCCAATAAATTGCGCT
TCCGCCCGCGCGCCTATTCGCATGTCGGCAAGGTGCCTATGCTCGATGTGTTCGACAAGCCGATCCGCGACTGGGATTCG
GTGGCGAAACGGCTATTCGACATCACCTTCAGCCTCATGGGGATCGCCCTTTTCTGGCCGGTGATGCTCGCTGCGGGAAT
TGCCGTGAAAGCGACCTCCAAAGGTCCGGTCCTCTTCAAGCAGAAGCGCCACGGCTTCAACAATGAGACCATCAACGTCT
GGAAGTTCCGTTCCATGTATACGGAGCTCAGCGATCCCACGGCGAAAAAAGCCGTCACGAAGAACGATCCGCGCGTCACA
CCGGTTGGCCGCTTCCTGCGCAAATCCTCGATGGACGAACTGCCGCAGCTTTTCAACGTTCTCTACGGCGACCTCTCTTT
GGTCGGCCCGCGCCCGCACGCCGTTCACGCGCAGACGGGCGATCTGAAATATACCGAGGTGGTGGAACATTATTTCGCCC
GCCACAAGGTCAAGCCCGGGGTGACCGGCTGGGCGCAGATCAATGGCTGGCGCGGCGAGATCGACCACGGCGACAAGATC
AAGTTCCGAACCGAATATGATCTCTATTACATCGAGAACTGGTCGCTGTTTCTCGATCTGAAGATCCTGTTCCTCACGCC
GATCCGGCTGCTGAAATCGGAAAATGCCTATTGA

Upstream 100 bases:

>100_bases
ACGTGACTGGGAGGCTCTACCACTTAGAAGTTGCGTATCGTCCGGTCGAAAATCGAATGCGAGTTTCGGGCCGATGCGCT
AGAGGGTGCGGACCAAAGCA

Downstream 100 bases:

>100_bases
CCCATACCGGTTACCAGCACACGGCGGATTTTGACGCACCCTTAGCCACCATGCGGCTCATCGGCGCGTTCTTCATCGCA
TTTGGGGTGTTTCTCTCCGG

Product: polysaccharide biosynthesis glycosyltransferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 517; Mature: 516

Protein sequence:

>517_residues
MTKTDKDRLQFDLASLRKKLSDYEAGGAAASETIVLNPLARQIANQLRAGNHSPNMVIGQLRLLEFTMLVIIAAVVNGIA
ADRGLETFLKVFSGGVLGAALCVFLLQAGDCYQLPTLRTPLKMFARIQGAVCLSFVGIACFLLVFFPETLQSWQAFAIWY
ATGAIAIFAGRLILGFAIRHWGRNGVMERRAVIVGGGDAARDLIRSLEQQSDNDIRICGIFDDRKSERSPEVVAGYPKLG
TFAELVEFARLTKLDMLIIALPLSAEARILQLLRKLWVLPVDIRIAAHANKLRFRPRAYSHVGKVPMLDVFDKPIRDWDS
VAKRLFDITFSLMGIALFWPVMLAAGIAVKATSKGPVLFKQKRHGFNNETINVWKFRSMYTELSDPTAKKAVTKNDPRVT
PVGRFLRKSSMDELPQLFNVLYGDLSLVGPRPHAVHAQTGDLKYTEVVEHYFARHKVKPGVTGWAQINGWRGEIDHGDKI
KFRTEYDLYYIENWSLFLDLKILFLTPIRLLKSENAY

Sequences:

>Translated_517_residues
MTKTDKDRLQFDLASLRKKLSDYEAGGAAASETIVLNPLARQIANQLRAGNHSPNMVIGQLRLLEFTMLVIIAAVVNGIA
ADRGLETFLKVFSGGVLGAALCVFLLQAGDCYQLPTLRTPLKMFARIQGAVCLSFVGIACFLLVFFPETLQSWQAFAIWY
ATGAIAIFAGRLILGFAIRHWGRNGVMERRAVIVGGGDAARDLIRSLEQQSDNDIRICGIFDDRKSERSPEVVAGYPKLG
TFAELVEFARLTKLDMLIIALPLSAEARILQLLRKLWVLPVDIRIAAHANKLRFRPRAYSHVGKVPMLDVFDKPIRDWDS
VAKRLFDITFSLMGIALFWPVMLAAGIAVKATSKGPVLFKQKRHGFNNETINVWKFRSMYTELSDPTAKKAVTKNDPRVT
PVGRFLRKSSMDELPQLFNVLYGDLSLVGPRPHAVHAQTGDLKYTEVVEHYFARHKVKPGVTGWAQINGWRGEIDHGDKI
KFRTEYDLYYIENWSLFLDLKILFLTPIRLLKSENAY
>Mature_516_residues
TKTDKDRLQFDLASLRKKLSDYEAGGAAASETIVLNPLARQIANQLRAGNHSPNMVIGQLRLLEFTMLVIIAAVVNGIAA
DRGLETFLKVFSGGVLGAALCVFLLQAGDCYQLPTLRTPLKMFARIQGAVCLSFVGIACFLLVFFPETLQSWQAFAIWYA
TGAIAIFAGRLILGFAIRHWGRNGVMERRAVIVGGGDAARDLIRSLEQQSDNDIRICGIFDDRKSERSPEVVAGYPKLGT
FAELVEFARLTKLDMLIIALPLSAEARILQLLRKLWVLPVDIRIAAHANKLRFRPRAYSHVGKVPMLDVFDKPIRDWDSV
AKRLFDITFSLMGIALFWPVMLAAGIAVKATSKGPVLFKQKRHGFNNETINVWKFRSMYTELSDPTAKKAVTKNDPRVTP
VGRFLRKSSMDELPQLFNVLYGDLSLVGPRPHAVHAQTGDLKYTEVVEHYFARHKVKPGVTGWAQINGWRGEIDHGDKIK
FRTEYDLYYIENWSLFLDLKILFLTPIRLLKSENAY

Specific function: Slime polysaccharide colanic acid biosynthesis. [C]

COG id: COG2148

COG function: function code M; Sugar transferases involved in lipopolysaccharide synthesis

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial sugar transferase family [H]

Homologues:

Organism=Escherichia coli, GI1788360, Length=373, Percent_Identity=37.8016085790885, Blast_Score=240, Evalue=2e-64,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003362
- InterPro:   IPR017475
- InterPro:   IPR017473 [H]

Pfam domain/function: PF02397 Bac_transf [H]

EC number: NA

Molecular weight: Translated: 58027; Mature: 57895

Theoretical pI: Translated: 10.13; Mature: 10.13

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKTDKDRLQFDLASLRKKLSDYEAGGAAASETIVLNPLARQIANQLRAGNHSPNMVIGQ
CCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEECHHHHHHHHHHHCCCCCCCHHHHH
LRLLEFTMLVIIAAVVNGIAADRGLETFLKVFSGGVLGAALCVFLLQAGDCYQLPTLRTP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCCCCHH
LKMFARIQGAVCLSFVGIACFLLVFFPETLQSWQAFAIWYATGAIAIFAGRLILGFAIRH
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
WGRNGVMERRAVIVGGGDAARDLIRSLEQQSDNDIRICGIFDDRKSERSPEVVAGYPKLG
HCCCCCCCCCEEEEECCHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCCCEEECCCCCC
TFAELVEFARLTKLDMLIIALPLSAEARILQLLRKLWVLPVDIRIAAHANKLRFRPRAYS
HHHHHHHHHHHHHHCEEEEEECCCCHHHHHHHHHHHCCCCEEEEEEECCCHHCCCCHHHH
HVGKVPMLDVFDKPIRDWDSVAKRLFDITFSLMGIALFWPVMLAAGIAVKATSKGPVLFK
HHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCEEEE
QKRHGFNNETINVWKFRSMYTELSDPTAKKAVTKNDPRVTPVGRFLRKSSMDELPQLFNV
HHHCCCCCCCEEEEEHHHHHHHHCCCHHHHCCCCCCCCCCHHHHHHHHCCHHHHHHHHHH
LYGDLSLVGPRPHAVHAQTGDLKYTEVVEHYFARHKVKPGVTGWAQINGWRGEIDHGDKI
HHCCCEEECCCCCEECCCCCCCHHHHHHHHHHHHCCCCCCCCCEEEECCCCCCCCCCCEE
KFRTEYDLYYIENWSLFLDLKILFLTPIRLLKSENAY
EEEEEEEEEEEECCEEEEEHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
TKTDKDRLQFDLASLRKKLSDYEAGGAAASETIVLNPLARQIANQLRAGNHSPNMVIGQ
CCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEECHHHHHHHHHHHCCCCCCCHHHHH
LRLLEFTMLVIIAAVVNGIAADRGLETFLKVFSGGVLGAALCVFLLQAGDCYQLPTLRTP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCCCCHH
LKMFARIQGAVCLSFVGIACFLLVFFPETLQSWQAFAIWYATGAIAIFAGRLILGFAIRH
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
WGRNGVMERRAVIVGGGDAARDLIRSLEQQSDNDIRICGIFDDRKSERSPEVVAGYPKLG
HCCCCCCCCCEEEEECCHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCCCEEECCCCCC
TFAELVEFARLTKLDMLIIALPLSAEARILQLLRKLWVLPVDIRIAAHANKLRFRPRAYS
HHHHHHHHHHHHHHCEEEEEECCCCHHHHHHHHHHHCCCCEEEEEEECCCHHCCCCHHHH
HVGKVPMLDVFDKPIRDWDSVAKRLFDITFSLMGIALFWPVMLAAGIAVKATSKGPVLFK
HHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCEEEE
QKRHGFNNETINVWKFRSMYTELSDPTAKKAVTKNDPRVTPVGRFLRKSSMDELPQLFNV
HHHCCCCCCCEEEEEHHHHHHHHCCCHHHHCCCCCCCCCCHHHHHHHHCCHHHHHHHHHH
LYGDLSLVGPRPHAVHAQTGDLKYTEVVEHYFARHKVKPGVTGWAQINGWRGEIDHGDKI
HHCCCEEECCCCCEECCCCCCCHHHHHHHHHHHHCCCCCCCCCEEEECCCCCCCCCCCEE
KFRTEYDLYYIENWSLFLDLKILFLTPIRLLKSENAY
EEEEEEEEEEEECCEEEEEHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8759852; 9097040; 9278503 [H]