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The map label for this gene is purN [H]

Identifier: 159184634

GI number: 159184634

Start: 1128956

End: 1129561

Strand: Reverse

Name: purN [H]

Synonym: Atu1140

Alternate gene names: 159184634

Gene position: 1129561-1128956 (Counterclockwise)

Preceding gene: 159184635

Following gene: 159184633

Centisome position: 39.75

GC content: 58.91

Gene sequence:

>606_bases
ATGGTGTCGCTGGCCAAGGCTTGTCAGGCCGCGGATTTTCCGGCCGAAATCGCCTGCGTGATCTCGGATAAGGCGTCCGC
GGGCGGGCTGGAAAAAGCCCGTGACCTCGGCATTCCGACGCTGGTGTTCGAACGCAAAACCTATGCAAGCAAGGCCGAAC
ATGAAGGCGCCATTCTGGCCGCACTTGGCGAGATCGCGCCTGATATCATCTGCCTTGCCGGGTACATGCGGCTGATATCG
GGGGATTTCATCGCCCCCTATGAAGGCCGGATCATCAATATCCACCCTTCCCTGCTGCCGCTCTTTCCCGGTCTTCACAC
CCATCAGCGCGCCATCGACAGCGGCATGAAGATTTCCGGCTGCACCGTGCATTTCGTCACCGAAGGCATGGATGAAGGCC
CAACCATCGCTCAAGGGGCGGTGCCGGTACTTTCCGGCGACACGGCCGAGACGCTTGCGGCACGCATCCTCACCGTCGAG
CACCAGCTTTACCCGCTTACGTTGAAGCGGCTTGCCGAAGGCAAGGTTCGGATGGAAGACGGCAAAGCCGTCTCCACGGA
CAATGAAAGTAAATCAGAGTATTCCAGCCTGATTTCCGCTTTCTAA

Upstream 100 bases:

>100_bases
ACGGCACGATCTACAAGGGCACTCTCGGCCTATGATGAGCGCCGCCTTGTCGTCCGGTCGCAAGCGTGTCGTCGTTTTCA
TCTCCGGCGGCGGCTCCAAC

Downstream 100 bases:

>100_bases
TAGAGTTACTTCTAAAGTACAAAAATAAATCAGAAGTGGTGTTTTTCGGGAAAATAAACCGATCATTTACCATAATCACT
CTTAATGCCCGCAACGGCCC

Product: phosphoribosylglycinamide formyltransferase

Products: NA

Alternate protein names: 5'-phosphoribosylglycinamide transformylase; GAR transformylase; GART [H]

Number of amino acids: Translated: 201; Mature: 201

Protein sequence:

>201_residues
MVSLAKACQAADFPAEIACVISDKASAGGLEKARDLGIPTLVFERKTYASKAEHEGAILAALGEIAPDIICLAGYMRLIS
GDFIAPYEGRIINIHPSLLPLFPGLHTHQRAIDSGMKISGCTVHFVTEGMDEGPTIAQGAVPVLSGDTAETLAARILTVE
HQLYPLTLKRLAEGKVRMEDGKAVSTDNESKSEYSSLISAF

Sequences:

>Translated_201_residues
MVSLAKACQAADFPAEIACVISDKASAGGLEKARDLGIPTLVFERKTYASKAEHEGAILAALGEIAPDIICLAGYMRLIS
GDFIAPYEGRIINIHPSLLPLFPGLHTHQRAIDSGMKISGCTVHFVTEGMDEGPTIAQGAVPVLSGDTAETLAARILTVE
HQLYPLTLKRLAEGKVRMEDGKAVSTDNESKSEYSSLISAF
>Mature_201_residues
MVSLAKACQAADFPAEIACVISDKASAGGLEKARDLGIPTLVFERKTYASKAEHEGAILAALGEIAPDIICLAGYMRLIS
GDFIAPYEGRIINIHPSLLPLFPGLHTHQRAIDSGMKISGCTVHFVTEGMDEGPTIAQGAVPVLSGDTAETLAARILTVE
HQLYPLTLKRLAEGKVRMEDGKAVSTDNESKSEYSSLISAF

Specific function: De novo purine biosynthesis; third step. [C]

COG id: COG0299

COG function: function code F; Folate-dependent phosphoribosylglycinamide formyltransferase PurN

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GART family [H]

Homologues:

Organism=Homo sapiens, GI4503915, Length=176, Percent_Identity=46.0227272727273, Blast_Score=169, Evalue=2e-42,
Organism=Homo sapiens, GI209869995, Length=176, Percent_Identity=46.0227272727273, Blast_Score=169, Evalue=2e-42,
Organism=Homo sapiens, GI209869993, Length=176, Percent_Identity=46.0227272727273, Blast_Score=169, Evalue=2e-42,
Organism=Escherichia coli, GI1788846, Length=183, Percent_Identity=39.8907103825137, Blast_Score=164, Evalue=5e-42,
Organism=Escherichia coli, GI1787483, Length=138, Percent_Identity=35.5072463768116, Blast_Score=90, Evalue=8e-20,
Organism=Caenorhabditis elegans, GI17567511, Length=165, Percent_Identity=40.6060606060606, Blast_Score=125, Evalue=1e-29,
Organism=Drosophila melanogaster, GI24582400, Length=160, Percent_Identity=45, Blast_Score=154, Evalue=5e-38,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002376
- InterPro:   IPR001555
- InterPro:   IPR004607 [H]

Pfam domain/function: PF00551 Formyl_trans_N [H]

EC number: =2.1.2.2 [H]

Molecular weight: Translated: 21354; Mature: 21354

Theoretical pI: Translated: 5.28; Mature: 5.28

Prosite motif: PS00373 GART

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVSLAKACQAADFPAEIACVISDKASAGGLEKARDLGIPTLVFERKTYASKAEHEGAILA
CCCHHHHHHCCCCCCEEEEEEECCCCCCCCHHHHHCCCCEEEECCHHHHHHHHCCCHHHH
ALGEIAPDIICLAGYMRLISGDFIAPYEGRIINIHPSLLPLFPGLHTHQRAIDSGMKISG
HHHHHHHHHHHHHHHHHHHCCCEECCCCCEEEEECCHHHHHCCCCHHHHHHHHCCCEECC
CTVHFVTEGMDEGPTIAQGAVPVLSGDTAETLAARILTVEHQLYPLTLKRLAEGKVRMED
EEEEEEECCCCCCCCCCCCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECC
GKAVSTDNESKSEYSSLISAF
CCEECCCCCCHHHHHHHHHCC
>Mature Secondary Structure
MVSLAKACQAADFPAEIACVISDKASAGGLEKARDLGIPTLVFERKTYASKAEHEGAILA
CCCHHHHHHCCCCCCEEEEEEECCCCCCCCHHHHHCCCCEEEECCHHHHHHHHCCCHHHH
ALGEIAPDIICLAGYMRLISGDFIAPYEGRIINIHPSLLPLFPGLHTHQRAIDSGMKISG
HHHHHHHHHHHHHHHHHHHCCCEECCCCCEEEEECCHHHHHCCCCHHHHHHHHCCCEECC
CTVHFVTEGMDEGPTIAQGAVPVLSGDTAETLAARILTVEHQLYPLTLKRLAEGKVRMED
EEEEEEECCCCCCCCCCCCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECC
GKAVSTDNESKSEYSSLISAF
CCEECCCCCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]