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The map label for this gene is ragB [H]

Identifier: 159184559

GI number: 159184559

Start: 968713

End: 970122

Strand: Direct

Name: ragB [H]

Synonym: Atu0979

Alternate gene names: 159184559

Gene position: 968713-970122 (Clockwise)

Preceding gene: 15888321

Following gene: 159184560

Centisome position: 34.09

GC content: 62.2

Gene sequence:

>1410_bases
ATGGCCCGCCTCAGAATATTGTTCAGATCGACAGCGGTGCGCCTTTCGGCGCTCTATATCCTGCTCTTCGCACTCTGCGC
CGCCTTCCTCGTCTTTTACGTCACCGCCCTGTCGGAACGGCTGCTGAACCAGCAGACGCGCGATGCCGTGTTGCAGGAAG
TCTCGGATGTGCAGCGCATCTATAATCGCGGCGGCATCAATCCGCTGCTGCGGATGATGGAGCGCCGCGCCCGCCAGCCG
GGCGCGAGCCTTTACATCATTGCCGGCCCGAACGGCGAAATCCTTGCCGGCAACGTCGCCTCCGTGCAGCCCGGCGTCTT
CGACAAGGAAGGCTGGACGGGCTTTCCGTTCCGTTACGAGCGTTATTCCGAAAGCGGCGACAGCGTGCAGCATCTCGCCA
CCGCCAATGTTTTCATGCTGGACAACGGTCTGCGCATCCTGATCGGCCGCGATCTTGGAGAGCCGACGAAGTTCCGGGCG
CTGGTGCGCAATGCGTTGATGGTGGCGCTTGCGATCATGAGCGGCGGTGCCTTGCTCATCTGGTTCGGCATTGGCCGCAA
CGCTCTGAAACGCATCGACCGCATGTCGGCGGCAACCAAGAGGATCATCGCCGGCGACCTTTCCCAGCGCCTGCCGCAGG
GCCGCTCCGGGGACGAATTCGACCGGCTGTCCGGCTCGCTCAACGCCATGCTCGGCCGTATCGAAAAGCTGAACGAGGGC
CTGCGCCAGGTTTCCGACAACATCGCCCATGACCTGAAGACGCCGCTCACACGGCTGCGCAACAAGGCGGCCGCCGCCCT
CGATGACAATGACGAGACGAAACGGCGCGCCGCTCTTGAAGGCATCATCGCCGAATCCGACCAGCTCATCCGCACCTTCA
ATGCACTGTTGATGATCTCCCGCGTTGAGGCAGGCTCGATCGCCGCCGAAATGACTGACGTGGACATGTCGGCCATCGCC
GCCGACAGCGCCGAGCTTTACGAGCCGGTGGCCGAGGATGCCGGCCTTGCGCTGGAATCGCAGATAGAGCCGGGCCTTTC
GGTGCAGGGCAACCGCGAACTGATCGGCCAGGCGCTTTCCAATCTCATCGACAATGCCATGAAATATGCCTGCGAAGGCG
AGGGCGACAAGAAACTCGTCGTCCGTCTGGTGAAAGAACCCGAAGCAATCGTGCTCTCTGTTGCCGATCATGGTCCCGGC
ATTCCCGCCGACAAGCGCGGTGAGGTGCTGAAACGCTTCGTGCGGCTGGACGAAAGCCGCTCCAAGCCGGGCACTGGGCT
TGGCCTCTCGCTGGTGGAAGCCGTGATGGAATTGCACGGCGGATCGCTGGTGCTCTCGGACACGGATGCCACCAATACGG
CCTGCCCGGGACTGACCATTTCGATGGTTTTTCCCATTTCGAAGCCTTAA

Upstream 100 bases:

>100_bases
CCGCCTGCGCTCGAAGATCGAGAAGGACTTCGAGAAACCGCTCTTGAAGACCATTCGCGGTGCCGGTTACATGATGAAGG
ACGAGGGCTGATTGGCGAAA

Downstream 100 bases:

>100_bases
CGGACACCTGTGGTTTTAGCACCGTTATTCTCGTGCGCGAAGACATGCTTGAGGCCGCCGCAGGTGCATGGGGCGGCATC
CTGTTGTAGATGTCAGATGC

Product: two component sensor kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 469; Mature: 468

Protein sequence:

>469_residues
MARLRILFRSTAVRLSALYILLFALCAAFLVFYVTALSERLLNQQTRDAVLQEVSDVQRIYNRGGINPLLRMMERRARQP
GASLYIIAGPNGEILAGNVASVQPGVFDKEGWTGFPFRYERYSESGDSVQHLATANVFMLDNGLRILIGRDLGEPTKFRA
LVRNALMVALAIMSGGALLIWFGIGRNALKRIDRMSAATKRIIAGDLSQRLPQGRSGDEFDRLSGSLNAMLGRIEKLNEG
LRQVSDNIAHDLKTPLTRLRNKAAAALDDNDETKRRAALEGIIAESDQLIRTFNALLMISRVEAGSIAAEMTDVDMSAIA
ADSAELYEPVAEDAGLALESQIEPGLSVQGNRELIGQALSNLIDNAMKYACEGEGDKKLVVRLVKEPEAIVLSVADHGPG
IPADKRGEVLKRFVRLDESRSKPGTGLGLSLVEAVMELHGGSLVLSDTDATNTACPGLTISMVFPISKP

Sequences:

>Translated_469_residues
MARLRILFRSTAVRLSALYILLFALCAAFLVFYVTALSERLLNQQTRDAVLQEVSDVQRIYNRGGINPLLRMMERRARQP
GASLYIIAGPNGEILAGNVASVQPGVFDKEGWTGFPFRYERYSESGDSVQHLATANVFMLDNGLRILIGRDLGEPTKFRA
LVRNALMVALAIMSGGALLIWFGIGRNALKRIDRMSAATKRIIAGDLSQRLPQGRSGDEFDRLSGSLNAMLGRIEKLNEG
LRQVSDNIAHDLKTPLTRLRNKAAAALDDNDETKRRAALEGIIAESDQLIRTFNALLMISRVEAGSIAAEMTDVDMSAIA
ADSAELYEPVAEDAGLALESQIEPGLSVQGNRELIGQALSNLIDNAMKYACEGEGDKKLVVRLVKEPEAIVLSVADHGPG
IPADKRGEVLKRFVRLDESRSKPGTGLGLSLVEAVMELHGGSLVLSDTDATNTACPGLTISMVFPISKP
>Mature_468_residues
ARLRILFRSTAVRLSALYILLFALCAAFLVFYVTALSERLLNQQTRDAVLQEVSDVQRIYNRGGINPLLRMMERRARQPG
ASLYIIAGPNGEILAGNVASVQPGVFDKEGWTGFPFRYERYSESGDSVQHLATANVFMLDNGLRILIGRDLGEPTKFRAL
VRNALMVALAIMSGGALLIWFGIGRNALKRIDRMSAATKRIIAGDLSQRLPQGRSGDEFDRLSGSLNAMLGRIEKLNEGL
RQVSDNIAHDLKTPLTRLRNKAAAALDDNDETKRRAALEGIIAESDQLIRTFNALLMISRVEAGSIAAEMTDVDMSAIAA
DSAELYEPVAEDAGLALESQIEPGLSVQGNRELIGQALSNLIDNAMKYACEGEGDKKLVVRLVKEPEAIVLSVADHGPGI
PADKRGEVLKRFVRLDESRSKPGTGLGLSLVEAVMELHGGSLVLSDTDATNTACPGLTISMVFPISKP

Specific function: Member of the two-component regulatory system irlR/irlS. May be involved in invasion of eukaryotic cells and heavy-metal resistance. Probably activates irlR by phosphorylation [H]

COG id: COG0642

COG function: function code T; Signal transduction histidine kinase

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 histidine kinase domain [H]

Homologues:

Organism=Escherichia coli, GI1788393, Length=285, Percent_Identity=24.9122807017544, Blast_Score=100, Evalue=3e-22,
Organism=Escherichia coli, GI1790346, Length=282, Percent_Identity=28.7234042553192, Blast_Score=98, Evalue=1e-21,
Organism=Escherichia coli, GI1787894, Length=281, Percent_Identity=28.1138790035587, Blast_Score=97, Evalue=3e-21,
Organism=Escherichia coli, GI1786783, Length=293, Percent_Identity=25.2559726962457, Blast_Score=90, Evalue=3e-19,
Organism=Escherichia coli, GI1788279, Length=258, Percent_Identity=25.1937984496124, Blast_Score=89, Evalue=8e-19,
Organism=Escherichia coli, GI1790551, Length=238, Percent_Identity=27.7310924369748, Blast_Score=84, Evalue=1e-17,
Organism=Escherichia coli, GI1786600, Length=240, Percent_Identity=27.9166666666667, Blast_Score=84, Evalue=1e-17,
Organism=Escherichia coli, GI1789808, Length=241, Percent_Identity=29.8755186721992, Blast_Score=84, Evalue=2e-17,
Organism=Escherichia coli, GI1786912, Length=200, Percent_Identity=28, Blast_Score=77, Evalue=3e-15,
Organism=Escherichia coli, GI1789403, Length=223, Percent_Identity=26.9058295964126, Blast_Score=68, Evalue=1e-12,
Organism=Escherichia coli, GI1790861, Length=298, Percent_Identity=25.1677852348993, Blast_Score=66, Evalue=6e-12,
Organism=Escherichia coli, GI87081816, Length=340, Percent_Identity=20.5882352941176, Blast_Score=65, Evalue=1e-11,
Organism=Escherichia coli, GI1789149, Length=331, Percent_Identity=22.3564954682779, Blast_Score=63, Evalue=5e-11,
Organism=Escherichia coli, GI48994928, Length=210, Percent_Identity=26.6666666666667, Blast_Score=62, Evalue=9e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR003660
- InterPro:   IPR004358
- InterPro:   IPR006290
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF00672 HAMP; PF02518 HATPase_c; PF00512 HisKA [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 50914; Mature: 50783

Theoretical pI: Translated: 6.55; Mature: 6.55

Prosite motif: PS50885 HAMP ; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MARLRILFRSTAVRLSALYILLFALCAAFLVFYVTALSERLLNQQTRDAVLQEVSDVQRI
CCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YNRGGINPLLRMMERRARQPGASLYIIAGPNGEILAGNVASVQPGVFDKEGWTGFPFRYE
HHCCCCHHHHHHHHHHHCCCCCEEEEEECCCCCEEECCCCCCCCCCCCCCCCCCCCCHHH
RYSESGDSVQHLATANVFMLDNGLRILIGRDLGEPTKFRALVRNALMVALAIMSGGALLI
HHCCCCCHHHHHHHCEEEEECCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHCCCEEEE
WFGIGRNALKRIDRMSAATKRIIAGDLSQRLPQGRSGDEFDRLSGSLNAMLGRIEKLNEG
EEECCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
LRQVSDNIAHDLKTPLTRLRNKAAAALDDNDETKRRAALEGIIAESDQLIRTFNALLMIS
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCHHHHHHHHHHHHHHH
RVEAGSIAAEMTDVDMSAIAADSAELYEPVAEDAGLALESQIEPGLSVQGNRELIGQALS
HCCCCHHHHHHHHCHHHHHHCCHHHHHHHHHHHCCCEEHHCCCCCCCCCCCHHHHHHHHH
NLIDNAMKYACEGEGDKKLVVRLVKEPEAIVLSVADHGPGIPADKRGEVLKRFVRLDESR
HHHHHHHHHHHCCCCCHHHHHHHHCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHCC
SKPGTGLGLSLVEAVMELHGGSLVLSDTDATNTACPGLTISMVFPISKP
CCCCCCCCHHHHHHHHHHCCCEEEEECCCCCCCCCCCCEEEEEECCCCC
>Mature Secondary Structure 
ARLRILFRSTAVRLSALYILLFALCAAFLVFYVTALSERLLNQQTRDAVLQEVSDVQRI
CCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YNRGGINPLLRMMERRARQPGASLYIIAGPNGEILAGNVASVQPGVFDKEGWTGFPFRYE
HHCCCCHHHHHHHHHHHCCCCCEEEEEECCCCCEEECCCCCCCCCCCCCCCCCCCCCHHH
RYSESGDSVQHLATANVFMLDNGLRILIGRDLGEPTKFRALVRNALMVALAIMSGGALLI
HHCCCCCHHHHHHHCEEEEECCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHCCCEEEE
WFGIGRNALKRIDRMSAATKRIIAGDLSQRLPQGRSGDEFDRLSGSLNAMLGRIEKLNEG
EEECCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
LRQVSDNIAHDLKTPLTRLRNKAAAALDDNDETKRRAALEGIIAESDQLIRTFNALLMIS
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCHHHHHHHHHHHHHHH
RVEAGSIAAEMTDVDMSAIAADSAELYEPVAEDAGLALESQIEPGLSVQGNRELIGQALS
HCCCCHHHHHHHHCHHHHHHCCHHHHHHHHHHHCCCEEHHCCCCCCCCCCCHHHHHHHHH
NLIDNAMKYACEGEGDKKLVVRLVKEPEAIVLSVADHGPGIPADKRGEVLKRFVRLDESR
HHHHHHHHHHHCCCCCHHHHHHHHCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHCC
SKPGTGLGLSLVEAVMELHGGSLVLSDTDATNTACPGLTISMVFPISKP
CCCCCCCCHHHHHHHHHHCCCEEEEECCCCCCCCCCCCEEEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9393784 [H]