Click here to switch to the map view.

The map label for this gene is bla [H]

Identifier: 159184536

GI number: 159184536

Start: 923289

End: 924113

Strand: Direct

Name: bla [H]

Synonym: Atu0933

Alternate gene names: 159184536

Gene position: 923289-924113 (Clockwise)

Preceding gene: 159184535

Following gene: 159184537

Centisome position: 32.49

GC content: 61.94

Gene sequence:

>825_bases
ATGCACTACCGGCTGCCCGCCATCGTTCTTTCGTGCCTTGCCCTTCCGGGCCTTCTGCCGCTTTCCGCTTTTGCCCAGCA
ACAGCCGCAGGCCTTTGAATGCACGCTGGTCACATCAATCGAAACGGGCGCCATCATCAACCAGCAGGGCGCCTGCGACC
AGCGCGTGGCGCCAGCCTCCACCTTCAAGGTGCCGTTGGCGCTGATCGGTTTCGATGCCGGTATTCTTCAGGATGGCAAG
ACGCCCGCCTGGGACTGGAAACCCGGCACTGAAGCCCGCGCTTCCGACAGAAAGACGGTCGATCCGACGATCTGGGAGCA
GGATTCGGTATTGTGGTATTCGCGCGAAATTACCCGCCGCCTCGGCCCGGAAAAATTCGCCGCCTATGTGAAGCGCCTCG
GTTACGGCAATGCCGATGTGTCCGGCGAACCGGGCAAGAATAACGGCCTCACCCATTCCTGGCTTGGCGCGTCACTTACG
ATTTCGCCGGTGGAGCAGGTGGGTTTCCTTCGCCGTCTTCTCGGCGGCAATCTGCCTTTCTCCCGCGATGCGCAGGCAAA
GACACGGGCCATCATGCCGGTCTTCGATGCGCCGGAAAGCTGGGCGGTGCACGGCAAGACCGGTACCGGCTACATGCGTG
ACGAAAAGGGCAATCCCGACCGTAACCGTCCCTTCGGCTGGTTTGTCGGTTGGGCGGAAAGAGAAGGCCAGCACATCGTC
TTTGCAAGGCTGCGTGTCTCCGACAAACCATCGAACGAGCCGCTCGGACCCGCCGTGCGCGATGCCTTCCTACGCGATAT
TCCGCGGCTGGCCGTGCATCGGTAG

Upstream 100 bases:

>100_bases
CATTTTCGTTTTGGTCCTATTCCTGCCCCATCCGTTACCTAAACCGCTCCGACCATTCCGGCAAATGATCCGCAAAAGCG
GACAAGGGACATCAAAACGC

Downstream 100 bases:

>100_bases
CAAGATCGTGACTTTCAGATTGCACATTTACCTGAAAAGCTCGGCGTGCGTTCCCGCGCGGGTGAAGATGAGAAGGTTCT
GCTTCTCATCGACTGTGTAG

Product: beta-lactamase class D

Products: NA

Alternate protein names: Penicillinase [H]

Number of amino acids: Translated: 274; Mature: 274

Protein sequence:

>274_residues
MHYRLPAIVLSCLALPGLLPLSAFAQQQPQAFECTLVTSIETGAIINQQGACDQRVAPASTFKVPLALIGFDAGILQDGK
TPAWDWKPGTEARASDRKTVDPTIWEQDSVLWYSREITRRLGPEKFAAYVKRLGYGNADVSGEPGKNNGLTHSWLGASLT
ISPVEQVGFLRRLLGGNLPFSRDAQAKTRAIMPVFDAPESWAVHGKTGTGYMRDEKGNPDRNRPFGWFVGWAEREGQHIV
FARLRVSDKPSNEPLGPAVRDAFLRDIPRLAVHR

Sequences:

>Translated_274_residues
MHYRLPAIVLSCLALPGLLPLSAFAQQQPQAFECTLVTSIETGAIINQQGACDQRVAPASTFKVPLALIGFDAGILQDGK
TPAWDWKPGTEARASDRKTVDPTIWEQDSVLWYSREITRRLGPEKFAAYVKRLGYGNADVSGEPGKNNGLTHSWLGASLT
ISPVEQVGFLRRLLGGNLPFSRDAQAKTRAIMPVFDAPESWAVHGKTGTGYMRDEKGNPDRNRPFGWFVGWAEREGQHIV
FARLRVSDKPSNEPLGPAVRDAFLRDIPRLAVHR
>Mature_274_residues
MHYRLPAIVLSCLALPGLLPLSAFAQQQPQAFECTLVTSIETGAIINQQGACDQRVAPASTFKVPLALIGFDAGILQDGK
TPAWDWKPGTEARASDRKTVDPTIWEQDSVLWYSREITRRLGPEKFAAYVKRLGYGNADVSGEPGKNNGLTHSWLGASLT
ISPVEQVGFLRRLLGGNLPFSRDAQAKTRAIMPVFDAPESWAVHGKTGTGYMRDEKGNPDRNRPFGWFVGWAEREGQHIV
FARLRVSDKPSNEPLGPAVRDAFLRDIPRLAVHR

Specific function: Has a broad substrate profile, hydrolyzes amoxicillin, ticarcillin, cephalothin, ceftazidime, cefotaxime, and aztreonam, but not imipenem or cephamycins [H]

COG id: COG2602

COG function: function code V; Beta-lactamase class D

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-D beta-lactamase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR002137
- InterPro:   IPR001460 [H]

Pfam domain/function: PF00905 Transpeptidase [H]

EC number: =3.5.2.6 [H]

Molecular weight: Translated: 30230; Mature: 30230

Theoretical pI: Translated: 9.46; Mature: 9.46

Prosite motif: PS00337 BETA_LACTAMASE_D

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHYRLPAIVLSCLALPGLLPLSAFAQQQPQAFECTLVTSIETGAIINQQGACDQRVAPAS
CCEEHHHHHHHHHHHCCCCHHHHHHHCCCCEEEEEEEEEECCCCEECCCCCCCCCCCCCC
TFKVPLALIGFDAGILQDGKTPAWDWKPGTEARASDRKTVDPTIWEQDSVLWYSREITRR
HHCCCEEEEECCCHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEHHHHHHH
LGPEKFAAYVKRLGYGNADVSGEPGKNNGLTHSWLGASLTISPVEQVGFLRRLLGGNLPF
CCHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEECCCCEEEECHHHHHHHHHHHHCCCCCC
SRDAQAKTRAIMPVFDAPESWAVHGKTGTGYMRDEKGNPDRNRPFGWFVGWAEREGQHIV
CCCCCHHHHEEEEEECCCCCCEEECCCCCCCCCCCCCCCCCCCCCEEEEEEECCCCCEEE
FARLRVSDKPSNEPLGPAVRDAFLRDIPRLAVHR
EEEEEECCCCCCCCCCHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MHYRLPAIVLSCLALPGLLPLSAFAQQQPQAFECTLVTSIETGAIINQQGACDQRVAPAS
CCEEHHHHHHHHHHHCCCCHHHHHHHCCCCEEEEEEEEEECCCCEECCCCCCCCCCCCCC
TFKVPLALIGFDAGILQDGKTPAWDWKPGTEARASDRKTVDPTIWEQDSVLWYSREITRR
HHCCCEEEEECCCHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEHHHHHHH
LGPEKFAAYVKRLGYGNADVSGEPGKNNGLTHSWLGASLTISPVEQVGFLRRLLGGNLPF
CCHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEECCCCEEEECHHHHHHHHHHHHCCCCCC
SRDAQAKTRAIMPVFDAPESWAVHGKTGTGYMRDEKGNPDRNRPFGWFVGWAEREGQHIV
CCCCCHHHHEEEEEECCCCCCEEECCCCCCCCCCCCCCCCCCCCCEEEEEEECCCCCEEE
FARLRVSDKPSNEPLGPAVRDAFLRDIPRLAVHR
EEEEEECCCCCCCCCCHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9333046 [H]