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The map label for this gene is barA [C]

Identifier: 159184529

GI number: 159184529

Start: 908847

End: 910379

Strand: Direct

Name: barA [C]

Synonym: Atu0921

Alternate gene names: 159184529

Gene position: 908847-910379 (Clockwise)

Preceding gene: 15888256

Following gene: 15888260

Centisome position: 31.98

GC content: 61.45

Gene sequence:

>1533_bases
ATGAGAGAAAAAGCGGTCGCACTGGTCGACCATGCCGCGGGCAGATGGCTTGCGCGCATGGAAGAGGACGCCGAAAGGCG
CGCCGGAACCATTGCACGCCTGCGCCGGCTGATCGCCTTCAGCGCCGTCGGCCTTTTTATTGTACCAGCCCTGTTCAGCC
TTGTTTTCAGCCCCGCTATCGCGCTGCCCATCGGCGCGGCCGTGGTGCTGGCGCTTTATCTCGCCGCCGCCGCGCTGCTG
ATTGCCTTTTCACGTCCCGCAGGCGGTTTCTCTTCGCCTTCGGCGCCTTCCGGCGATGAGGATATGATCGCCGCCGCGCA
GGTGCCCGGCCTCGTTCTCACCCTCAATGAAAACGGTCTCGTCGAGCGCGTCTCCGGCCGCGACCGCGACAGTTTCCCCG
CTGATCTCAAGGCCAGCAAGGGCGAGATCTTTGCTGAATATGTCTACGTCTCCGATCGCATCGAACTGATGCAGGCTTTC
GACCTGCTGCGGCAGGGCGAGGACAAGGCTACGGCCGAGCTTCGGTTCGAAACGGGAGCAAAATCGCGCCAGACGCAATT
CATGCACGTCCGTATCGAGATGACTGCAATCCGCGGCGCGACCGGCCGCCTGCGCCGTATCGTCGCCCAGCTTTCAGATG
TCACTGAGATGGAGCGCCTTCGCCGGGACGTTGCCCGCAAGGCGGCGGAGGCGGAATCGGCCAATGACGCCAAGTCGCGT
TTCCTTGCCGCCGTCAGCCATGAGCTGCGCACGCCGCTCAACGCCGTCCTCGGTTTTTCCGATATTCTGGCCGGCGAATA
TTTCGGCCGGCTGGAGAATGACCGCCAGCGGGAATATGTCGGCCTGATCCGCCAGTCCGGCGCGCATCTCCTGTCCGTCG
TCAACACCATGCTGGATATGAGCAAGCTCGAAGCCGGTCGTTACGAATTGCTGATGGAAAGCTTCCCGATTTCGGAGACC
ATAGCGTCCTGCGAAGCCATGCTCGGCCTGCAGGCGAAGGAAAAAGGCCTGACATTGACCAGCCGCATCCAGCGCGGTAT
CGGCGAAATCTCGGCCGATCAGCGCGCCATCCGGCAGGTCCTCATTAATCTCGCCGGCAATGCCATCAAATTCACCGATG
CGGGTGGTGTGGTGTCAATCGACGCCGCGCGTGAAGGTGGGATGCTGAAGCTGACGGTGAGCGATACCGGCATCGGCATC
GCATCCGACAAGATCGAATTGCTGGGCCAGCCTTTCATGCAGGTTCAGAATGAATATACCCGTCGCTATGAGGGCACGGG
TCTGGGACTGTCCCTGGTCAAGGGGCTTGTGGCACTGCATGGCGGCACTTTCGTCATCACCAGCAAGCCCGCCGAGGGAA
CGGTCGTAACGATCACCTTGCCTGCCGACGGTTCCGGCATGGTAGGTGGCGGTGAAAACGCTGAAACCGAATGCATGGTG
GAATTCCCGCCGCGCATCCGCCAACTCGGCGACATGGCTGCGAAGATGAAGAAGGATGGTGACGATGGCCCCGCGAAAGC
GAAAATCGCCTAA

Upstream 100 bases:

>100_bases
GCCTTTGGCGCCATTCTTTTTATCCATTCCTTAAGCCGCCGCCTTCTATTGTCGAGGCCGTTGGAACCGCCTTTAAGATT
CGGGTTTGGTATTGCGTAAT

Downstream 100 bases:

>100_bases
GAAAACAACGGCCCAGATCGGCGCCGGATTTGTTTCCTTGGTGGTTTCGGCCATCGGGCGCGAAATCCTGCGCCATCCGA
AACTGGTCGGCGGCTGCGGT

Product: two component sensor kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 510; Mature: 510

Protein sequence:

>510_residues
MREKAVALVDHAAGRWLARMEEDAERRAGTIARLRRLIAFSAVGLFIVPALFSLVFSPAIALPIGAAVVLALYLAAAALL
IAFSRPAGGFSSPSAPSGDEDMIAAAQVPGLVLTLNENGLVERVSGRDRDSFPADLKASKGEIFAEYVYVSDRIELMQAF
DLLRQGEDKATAELRFETGAKSRQTQFMHVRIEMTAIRGATGRLRRIVAQLSDVTEMERLRRDVARKAAEAESANDAKSR
FLAAVSHELRTPLNAVLGFSDILAGEYFGRLENDRQREYVGLIRQSGAHLLSVVNTMLDMSKLEAGRYELLMESFPISET
IASCEAMLGLQAKEKGLTLTSRIQRGIGEISADQRAIRQVLINLAGNAIKFTDAGGVVSIDAAREGGMLKLTVSDTGIGI
ASDKIELLGQPFMQVQNEYTRRYEGTGLGLSLVKGLVALHGGTFVITSKPAEGTVVTITLPADGSGMVGGGENAETECMV
EFPPRIRQLGDMAAKMKKDGDDGPAKAKIA

Sequences:

>Translated_510_residues
MREKAVALVDHAAGRWLARMEEDAERRAGTIARLRRLIAFSAVGLFIVPALFSLVFSPAIALPIGAAVVLALYLAAAALL
IAFSRPAGGFSSPSAPSGDEDMIAAAQVPGLVLTLNENGLVERVSGRDRDSFPADLKASKGEIFAEYVYVSDRIELMQAF
DLLRQGEDKATAELRFETGAKSRQTQFMHVRIEMTAIRGATGRLRRIVAQLSDVTEMERLRRDVARKAAEAESANDAKSR
FLAAVSHELRTPLNAVLGFSDILAGEYFGRLENDRQREYVGLIRQSGAHLLSVVNTMLDMSKLEAGRYELLMESFPISET
IASCEAMLGLQAKEKGLTLTSRIQRGIGEISADQRAIRQVLINLAGNAIKFTDAGGVVSIDAAREGGMLKLTVSDTGIGI
ASDKIELLGQPFMQVQNEYTRRYEGTGLGLSLVKGLVALHGGTFVITSKPAEGTVVTITLPADGSGMVGGGENAETECMV
EFPPRIRQLGDMAAKMKKDGDDGPAKAKIA
>Mature_510_residues
MREKAVALVDHAAGRWLARMEEDAERRAGTIARLRRLIAFSAVGLFIVPALFSLVFSPAIALPIGAAVVLALYLAAAALL
IAFSRPAGGFSSPSAPSGDEDMIAAAQVPGLVLTLNENGLVERVSGRDRDSFPADLKASKGEIFAEYVYVSDRIELMQAF
DLLRQGEDKATAELRFETGAKSRQTQFMHVRIEMTAIRGATGRLRRIVAQLSDVTEMERLRRDVARKAAEAESANDAKSR
FLAAVSHELRTPLNAVLGFSDILAGEYFGRLENDRQREYVGLIRQSGAHLLSVVNTMLDMSKLEAGRYELLMESFPISET
IASCEAMLGLQAKEKGLTLTSRIQRGIGEISADQRAIRQVLINLAGNAIKFTDAGGVVSIDAAREGGMLKLTVSDTGIGI
ASDKIELLGQPFMQVQNEYTRRYEGTGLGLSLVKGLVALHGGTFVITSKPAEGTVVTITLPADGSGMVGGGENAETECMV
EFPPRIRQLGDMAAKMKKDGDDGPAKAKIA

Specific function: Member Of The Two-Component Regulatory System Uvry/Bara Involved In The Regulation Of Carbon Metabolism Via The Csra/Csrb Regulatory System. Phosphorylates Uvry, Probably Via A Four-Step Phosphorelay. [C]

COG id: COG0642

COG function: function code T; Signal transduction histidine kinase

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PAC (PAS-associated C-terminal) domain [H]

Homologues:

Organism=Escherichia coli, GI1789149, Length=293, Percent_Identity=35.1535836177474, Blast_Score=143, Evalue=2e-35,
Organism=Escherichia coli, GI145693157, Length=259, Percent_Identity=32.4324324324324, Blast_Score=128, Evalue=8e-31,
Organism=Escherichia coli, GI48994928, Length=252, Percent_Identity=31.7460317460317, Blast_Score=124, Evalue=2e-29,
Organism=Escherichia coli, GI1788713, Length=323, Percent_Identity=28.7925696594427, Blast_Score=120, Evalue=2e-28,
Organism=Escherichia coli, GI87081816, Length=279, Percent_Identity=33.6917562724014, Blast_Score=117, Evalue=1e-27,
Organism=Escherichia coli, GI1786912, Length=241, Percent_Identity=33.195020746888, Blast_Score=108, Evalue=8e-25,
Organism=Escherichia coli, GI1790436, Length=223, Percent_Identity=28.6995515695067, Blast_Score=99, Evalue=6e-22,
Organism=Escherichia coli, GI1786600, Length=240, Percent_Identity=29.5833333333333, Blast_Score=97, Evalue=3e-21,
Organism=Escherichia coli, GI1788549, Length=309, Percent_Identity=23.3009708737864, Blast_Score=88, Evalue=1e-18,
Organism=Escherichia coli, GI1788393, Length=251, Percent_Identity=26.2948207171315, Blast_Score=82, Evalue=7e-17,
Organism=Escherichia coli, GI1786783, Length=280, Percent_Identity=25, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI87082128, Length=227, Percent_Identity=24.6696035242291, Blast_Score=74, Evalue=2e-14,
Organism=Escherichia coli, GI1790346, Length=230, Percent_Identity=26.0869565217391, Blast_Score=74, Evalue=2e-14,
Organism=Escherichia coli, GI1789808, Length=252, Percent_Identity=25.7936507936508, Blast_Score=63, Evalue=6e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR001610
- InterPro:   IPR000700
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF02518 HATPase_c; PF00512 HisKA [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 54814; Mature: 54814

Theoretical pI: Translated: 6.41; Mature: 6.41

Prosite motif: PS50113 PAC ; PS00107 PROTEIN_KINASE_ATP ; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MREKAVALVDHAAGRWLARMEEDAERRAGTIARLRRLIAFSAVGLFIVPALFSLVFSPAI
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHH
ALPIGAAVVLALYLAAAALLIAFSRPAGGFSSPSAPSGDEDMIAAAQVPGLVLTLNENGL
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHEEHCCCCCEEEEECCCCC
VERVSGRDRDSFPADLKASKGEIFAEYVYVSDRIELMQAFDLLRQGEDKATAELRFETGA
EEECCCCCCCCCCCCCCCCCCCEEEEEEEHHHHHHHHHHHHHHHCCCCCCEEEEEECCCC
KSRQTQFMHVRIEMTAIRGATGRLRRIVAQLSDVTEMERLRRDVARKAAEAESANDAKSR
CCCCCEEEEEEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHH
FLAAVSHELRTPLNAVLGFSDILAGEYFGRLENDRQREYVGLIRQSGAHLLSVVNTMLDM
HHHHHHHHHHCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCHHHHHHHHHHHHH
SKLEAGRYELLMESFPISETIASCEAMLGLQAKEKGLTLTSRIQRGIGEISADQRAIRQV
HHHCCCHHHHHHHHCCHHHHHHHHHHHHCCCHHHCCCHHHHHHHHHHHHCCHHHHHHHHH
LINLAGNAIKFTDAGGVVSIDAAREGGMLKLTVSDTGIGIASDKIELLGQPFMQVQNEYT
HHHHCCCEEEEECCCCEEEEECCCCCCEEEEEEECCCCCCCCHHHHHHCCHHHHHHHHHH
RRYEGTGLGLSLVKGLVALHGGTFVITSKPAEGTVVTITLPADGSGMVGGGENAETECMV
HHHCCCCCCHHHHHHHHHHCCCEEEEECCCCCCEEEEEEECCCCCCCCCCCCCCCCEEEE
EFPPRIRQLGDMAAKMKKDGDDGPAKAKIA
ECCHHHHHHHHHHHHHHCCCCCCCCCCCCC
>Mature Secondary Structure
MREKAVALVDHAAGRWLARMEEDAERRAGTIARLRRLIAFSAVGLFIVPALFSLVFSPAI
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHH
ALPIGAAVVLALYLAAAALLIAFSRPAGGFSSPSAPSGDEDMIAAAQVPGLVLTLNENGL
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHEEHCCCCCEEEEECCCCC
VERVSGRDRDSFPADLKASKGEIFAEYVYVSDRIELMQAFDLLRQGEDKATAELRFETGA
EEECCCCCCCCCCCCCCCCCCCEEEEEEEHHHHHHHHHHHHHHHCCCCCCEEEEEECCCC
KSRQTQFMHVRIEMTAIRGATGRLRRIVAQLSDVTEMERLRRDVARKAAEAESANDAKSR
CCCCCEEEEEEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHH
FLAAVSHELRTPLNAVLGFSDILAGEYFGRLENDRQREYVGLIRQSGAHLLSVVNTMLDM
HHHHHHHHHHCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCHHHHHHHHHHHHH
SKLEAGRYELLMESFPISETIASCEAMLGLQAKEKGLTLTSRIQRGIGEISADQRAIRQV
HHHCCCHHHHHHHHCCHHHHHHHHHHHHCCCHHHCCCHHHHHHHHHHHHCCHHHHHHHHH
LINLAGNAIKFTDAGGVVSIDAAREGGMLKLTVSDTGIGIASDKIELLGQPFMQVQNEYT
HHHHCCCEEEEECCCCEEEEECCCCCCEEEEEEECCCCCCCCHHHHHHCCHHHHHHHHHH
RRYEGTGLGLSLVKGLVALHGGTFVITSKPAEGTVVTITLPADGSGMVGGGENAETECMV
HHHCCCCCCHHHHHHHHHHCCCEEEEECCCCCCEEEEEEECCCCCCCCCCCCCCCCEEEE
EFPPRIRQLGDMAAKMKKDGDDGPAKAKIA
ECCHHHHHHHHHHHHHHCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11481430; 7756693 [H]