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The map label for this gene is yieO [C]

Identifier: 159184489

GI number: 159184489

Start: 836902

End: 838425

Strand: Direct

Name: yieO [C]

Synonym: Atu0838

Alternate gene names: 159184489

Gene position: 836902-838425 (Clockwise)

Preceding gene: 159184487

Following gene: 159184490

Centisome position: 29.45

GC content: 62.99

Gene sequence:

>1524_bases
ATGTCCACCGCCCCCGTGGCGCCGCTTGTGTCGGATCACCGCCGCAGGCTCATCGTTTTTCTGTTTCTGATGCTGGCCAT
GTTCATGGCCACACTCGACAACCAGATCGTCTCGACAGCGCTCCCCACCATCGTCGGTGAATTCGGCGCGCTGGAGCGTT
TCGGCTGGATTGGCTCGGCCTATCTTCTGGCGACCAGCGCGGTCATGCCCGTTTATGGCAAGCTCGGTGATCTCTTCGGC
CGCAAATATGTGATGATTGCGGCGGTCGTCATCTTCACGCTCGGTTCGCTCGCCTGCGGGCTGGCATGGTCTATGGACAG
CCTGATCGCGGCCCGTGTGCTTCAAGGGCTTGGCGGCGGCGGCATCATGGTGTCGATCTTCTCCGTTAATGCGGATCTGT
TCGAGCCACGCGAGCGCGCCCGCTACCAAAGCTATTCCAGCCTCACCATCATGGCCTCGGGCAGTGTCGGCCCGATTCTC
GGCGGGACCATGAGCGACCTGTTCGGCTGGCGGTCGATCTTCCTTATCAATCTGCCGCTCGGTATTATCGTCATCGCCGG
CCTTGCTCTCATGCTGCCCTATCGTCGCCCGGCGAGACAGCCAAAGATCGATTATCTCGGTGCGGTTCTTCTGGCCGCCA
CCATTGCCAGCGTGGTGTTCTGGGCCGATAGCAGCGAACTGTTCGGCTCGCTGATCGCGGCCCCAAGCCTCGGCATCATC
GCCTTTGCCGTCATCGCCGCTTTCCTGTGGGTGCAGGTGGAGCGTCGCGCGCCGGAACCCGTCGTGCCGCTGCGTCTCTT
CAAGGACAGCACCTTTCCGCTGTTGATGATCGTCTCGCTGACCAGCGGCGGCATCGGTATCGGCATGGTCAATTATTACG
CGCTGTTCCTGCAGACCACGACCGGGCTTTCGCCCTCCCATGCCGGCCTGTTCTTCATCGCCGTCACCGGCGGCATCGTC
ATGGGTTCGTTGTCTGCCGGACGGCTGATCTCGATTACCGGCGTCTACAAGCCCTTCTCGGTGGCCGGCCTCACCATCAA
TGTCCTCGTCATGCTGCTCTTCACGCAGATGCATGCCGGAACGCCGCTTTGGCTGATCGCGGTGCTGATGCTGGCGCAGG
GTTTCGCCGTCGGCCTCGGCCAGCAGGCACCCATCATCGGCGTACAGAACTCCGCTCCGAAGGCCGATATCGGCGCGGCA
AGCGGCGCGGTGACCCTGACCCGCATGGGCGGCGCCGCCATCGCCATCTCGGTCTATGGCGCCATCGTGTCATCAAGCTT
GAAAGGTGTCGCGATCGATATTCCCGGTGTCGGCAGGATCGAGGAACTGACGCCGAAAATGCTCGCCGAACTGCCCGCAA
CCTCCCAGGCCGCCGTCGCCTCGCTTTATTCGGATGCGTTTACGCCGCTATTCTTCGCGGCCGCCGCCACCGCCGCGATT
GGTCTTGCCGCGGCGTTGATGCTGAAACCGGTGCGCCTGCCGGCGGCGGTTGAAGCGAAGCCGGCGGAAGCAGCGGGAGA
GTAG

Upstream 100 bases:

>100_bases
ATAGCACAGATGTCAAAAACTTGCCATTGACATATATATGCCAATAGCACACAAATTGTCGCAGCAAAATCGCCGGAACT
CAACGAGATTTTTCATGGAT

Downstream 100 bases:

>100_bases
GAACGATTGCACGAAGGTGTGTCGGTTCCCGCGTGCACTTTCGTCCGTCTGCTCGCTCTTCCCTCATCCCTGTGCCTGTC
ACAGGGATCCAGCCAGCCCA

Product: MFS permease

Products: Proton [Cytoplasm]; drug [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 507; Mature: 506

Protein sequence:

>507_residues
MSTAPVAPLVSDHRRRLIVFLFLMLAMFMATLDNQIVSTALPTIVGEFGALERFGWIGSAYLLATSAVMPVYGKLGDLFG
RKYVMIAAVVIFTLGSLACGLAWSMDSLIAARVLQGLGGGGIMVSIFSVNADLFEPRERARYQSYSSLTIMASGSVGPIL
GGTMSDLFGWRSIFLINLPLGIIVIAGLALMLPYRRPARQPKIDYLGAVLLAATIASVVFWADSSELFGSLIAAPSLGII
AFAVIAAFLWVQVERRAPEPVVPLRLFKDSTFPLLMIVSLTSGGIGIGMVNYYALFLQTTTGLSPSHAGLFFIAVTGGIV
MGSLSAGRLISITGVYKPFSVAGLTINVLVMLLFTQMHAGTPLWLIAVLMLAQGFAVGLGQQAPIIGVQNSAPKADIGAA
SGAVTLTRMGGAAIAISVYGAIVSSSLKGVAIDIPGVGRIEELTPKMLAELPATSQAAVASLYSDAFTPLFFAAAATAAI
GLAAALMLKPVRLPAAVEAKPAEAAGE

Sequences:

>Translated_507_residues
MSTAPVAPLVSDHRRRLIVFLFLMLAMFMATLDNQIVSTALPTIVGEFGALERFGWIGSAYLLATSAVMPVYGKLGDLFG
RKYVMIAAVVIFTLGSLACGLAWSMDSLIAARVLQGLGGGGIMVSIFSVNADLFEPRERARYQSYSSLTIMASGSVGPIL
GGTMSDLFGWRSIFLINLPLGIIVIAGLALMLPYRRPARQPKIDYLGAVLLAATIASVVFWADSSELFGSLIAAPSLGII
AFAVIAAFLWVQVERRAPEPVVPLRLFKDSTFPLLMIVSLTSGGIGIGMVNYYALFLQTTTGLSPSHAGLFFIAVTGGIV
MGSLSAGRLISITGVYKPFSVAGLTINVLVMLLFTQMHAGTPLWLIAVLMLAQGFAVGLGQQAPIIGVQNSAPKADIGAA
SGAVTLTRMGGAAIAISVYGAIVSSSLKGVAIDIPGVGRIEELTPKMLAELPATSQAAVASLYSDAFTPLFFAAAATAAI
GLAAALMLKPVRLPAAVEAKPAEAAGE
>Mature_506_residues
STAPVAPLVSDHRRRLIVFLFLMLAMFMATLDNQIVSTALPTIVGEFGALERFGWIGSAYLLATSAVMPVYGKLGDLFGR
KYVMIAAVVIFTLGSLACGLAWSMDSLIAARVLQGLGGGGIMVSIFSVNADLFEPRERARYQSYSSLTIMASGSVGPILG
GTMSDLFGWRSIFLINLPLGIIVIAGLALMLPYRRPARQPKIDYLGAVLLAATIASVVFWADSSELFGSLIAAPSLGIIA
FAVIAAFLWVQVERRAPEPVVPLRLFKDSTFPLLMIVSLTSGGIGIGMVNYYALFLQTTTGLSPSHAGLFFIAVTGGIVM
GSLSAGRLISITGVYKPFSVAGLTINVLVMLLFTQMHAGTPLWLIAVLMLAQGFAVGLGQQAPIIGVQNSAPKADIGAAS
GAVTLTRMGGAAIAISVYGAIVSSSLKGVAIDIPGVGRIEELTPKMLAELPATSQAAVASLYSDAFTPLFFAAAATAAIG
LAAALMLKPVRLPAAVEAKPAEAAGE

Specific function: Unknown

COG id: COG0477

COG function: function code GEPR; Permeases of the major facilitator superfamily

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. TCR/tet family [H]

Homologues:

Organism=Escherichia coli, GI1790195, Length=453, Percent_Identity=30.0220750551876, Blast_Score=112, Evalue=6e-26,
Organism=Escherichia coli, GI1788392, Length=307, Percent_Identity=34.8534201954397, Blast_Score=105, Evalue=8e-24,
Organism=Escherichia coli, GI87081983, Length=308, Percent_Identity=29.2207792207792, Blast_Score=105, Evalue=9e-24,
Organism=Escherichia coli, GI1789042, Length=290, Percent_Identity=28.2758620689655, Blast_Score=105, Evalue=9e-24,
Organism=Escherichia coli, GI1788710, Length=291, Percent_Identity=31.9587628865979, Blast_Score=100, Evalue=2e-22,
Organism=Escherichia coli, GI1790146, Length=184, Percent_Identity=31.5217391304348, Blast_Score=72, Evalue=1e-13,
Organism=Saccharomyces cerevisiae, GI6323735, Length=326, Percent_Identity=29.4478527607362, Blast_Score=122, Evalue=9e-29,
Organism=Saccharomyces cerevisiae, GI6322958, Length=518, Percent_Identity=24.1312741312741, Blast_Score=105, Evalue=1e-23,
Organism=Saccharomyces cerevisiae, GI6319770, Length=430, Percent_Identity=23.7209302325581, Blast_Score=94, Evalue=6e-20,
Organism=Saccharomyces cerevisiae, GI6325455, Length=203, Percent_Identity=30.5418719211823, Blast_Score=93, Evalue=1e-19,
Organism=Saccharomyces cerevisiae, GI6321663, Length=149, Percent_Identity=35.5704697986577, Blast_Score=92, Evalue=2e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR005829
- InterPro:   IPR001958
- InterPro:   IPR011991 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 52820; Mature: 52689

Theoretical pI: Translated: 9.55; Mature: 9.55

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTAPVAPLVSDHRRRLIVFLFLMLAMFMATLDNQIVSTALPTIVGEFGALERFGWIGSA
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHH
YLLATSAVMPVYGKLGDLFGRKYVMIAAVVIFTLGSLACGLAWSMDSLIAARVLQGLGGG
HHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
GIMVSIFSVNADLFEPRERARYQSYSSLTIMASGSVGPILGGTMSDLFGWRSIFLINLPL
CEEEEEEECCCHHCCCHHHHHHCCCCCEEEEECCCCCCCCCCHHHHHHHHHHHEEEECCH
GIIVIAGLALMLPYRRPARQPKIDYLGAVLLAATIASVVFWADSSELFGSLIAAPSLGII
HHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCHHHH
AFAVIAAFLWVQVERRAPEPVVPLRLFKDSTFPLLMIVSLTSGGIGIGMVNYYALFLQTT
HHHHHHHHHHHHHHCCCCCCCCCCEEECCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHC
TGLSPSHAGLFFIAVTGGIVMGSLSAGRLISITGVYKPFSVAGLTINVLVMLLFTQMHAG
CCCCCCCCCEEEEEECCCHHEECCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHCCC
TPLWLIAVLMLAQGFAVGLGQQAPIIGVQNSAPKADIGAASGAVTLTRMGGAAIAISVYG
CHHHHHHHHHHHCCHHCCCCCCCCEEEECCCCCCCCCCCCCCCEEEEECCCCEEHHHHHH
AIVSSSLKGVAIDIPGVGRIEELTPKMLAELPATSQAAVASLYSDAFTPLFFAAAATAAI
HHHHCCCCCEEEECCCCCCHHHHCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
GLAAALMLKPVRLPAAVEAKPAEAAGE
HHHHHHHHCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
STAPVAPLVSDHRRRLIVFLFLMLAMFMATLDNQIVSTALPTIVGEFGALERFGWIGSA
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHH
YLLATSAVMPVYGKLGDLFGRKYVMIAAVVIFTLGSLACGLAWSMDSLIAARVLQGLGGG
HHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
GIMVSIFSVNADLFEPRERARYQSYSSLTIMASGSVGPILGGTMSDLFGWRSIFLINLPL
CEEEEEEECCCHHCCCHHHHHHCCCCCEEEEECCCCCCCCCCHHHHHHHHHHHEEEECCH
GIIVIAGLALMLPYRRPARQPKIDYLGAVLLAATIASVVFWADSSELFGSLIAAPSLGII
HHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCHHHH
AFAVIAAFLWVQVERRAPEPVVPLRLFKDSTFPLLMIVSLTSGGIGIGMVNYYALFLQTT
HHHHHHHHHHHHHHCCCCCCCCCCEEECCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHC
TGLSPSHAGLFFIAVTGGIVMGSLSAGRLISITGVYKPFSVAGLTINVLVMLLFTQMHAG
CCCCCCCCCEEEEEECCCHHEECCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHCCC
TPLWLIAVLMLAQGFAVGLGQQAPIIGVQNSAPKADIGAASGAVTLTRMGGAAIAISVYG
CHHHHHHHHHHHCCHHCCCCCCCCEEEECCCCCCCCCCCCCCCEEEEECCCCEEHHHHHH
AIVSSSLKGVAIDIPGVGRIEELTPKMLAELPATSQAAVASLYSDAFTPLFFAAAATAAI
HHHHCCCCCEEEECCCCCCHHHHCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
GLAAALMLKPVRLPAAVEAKPAEAAGE
HHHHHHHHCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; drug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + drug [Cytoplasm] = Proton [Cytoplasm] + drug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036 [H]