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The map label for this gene is cysT [H]

Identifier: 159184479

GI number: 159184479

Start: 819388

End: 820245

Strand: Direct

Name: cysT [H]

Synonym: Atu0821

Alternate gene names: 159184479

Gene position: 819388-820245 (Clockwise)

Preceding gene: 159184478

Following gene: 15888163

Centisome position: 28.84

GC content: 58.51

Gene sequence:

>858_bases
ATGAGCAATAATACATCCGGAAACAGGTGGAAATGGCGGCAGTCGAGCGTCATACCCGGCTTCGGCCTGACGTTTGGTTA
CACTGTCACCTATCTGTTTCTCATCATTCTTATTCCGCTCGGCGGCCTTGTCTGGTCCACGGCGAAACTCGGTTTTGCAG
ATTTCATTGCGATTGCCACCGATAGCCGCACGCTGAATGCGCTGCGTGTCAGCTTCGGCACGGCCTTCATTGCCGCGCTG
GTCAACGCCGTTTTCGGCGTCATCGTCGCCTGGGTGCTGACGCGGTATCGTTTCCCCGGTCGCCGTTTCGTCGATGCCAT
CGTCGATCTGCCCTTCGCCCTGCCGACGGCGGTGGCAGGTATTGCACTCACCACGCTTTATGCCAATCGCGGCTGGGTCG
GCTCGCTATTCGAACCTTTCGGCATCAAGATCGCCTTTACGCCAACCGGCATCGTCATCGCGCTGATCTTCATCGGCCTG
CCTTTCGTGGTCCGCACCGTGCAGCCGGTCATGGAAGAGATCGACCGTCAGGTGGAGGAAGTGGCGGCGACGCTTGGCGC
CAACCGTTTCCAGACCATCACCCGTGTTCTGCTGCCGAGCCTCACGCCCGCCATCCTCACGGGTTTTGCGCTCGCCTTCG
CGCGTGGCGTTGGCGAATATGGTTCGGTTATCTTCATCGCCGGCAATATCCCTTACGTCTCGGAAATCGCACCGCTCCTC
ATCGTCATCCGGCTGGAGGAGTTCAATTATGCGGCAGCCACCGGCATCGCCACCATCATGCTGATCATCTCGTTTGCCAT
GCTGTTCCTCATCAATCTCATTCAGGCCTGGAGCCGCAAGAGGTACGGTTATGTCTGA

Upstream 100 bases:

>100_bases
AACGCCGGGGAACACCGGCTCCCAGAGGGCATTTCCAGGAAAAGTGGACCCCGGTTTCCTGTCCTGAAAATGTACAAAAC
AAAGATTATCCGGAGCCTTG

Downstream 100 bases:

>100_bases
GACCGCTTCCCGCACCTCGCCCCGGCCATTCCGCGATCCCGCAAGCGAAAGCCTTCCCGCGCGGCTGGCGCTGATCGCCG
TTGCCTTCCTGTTCCTCGCG

Product: ABC transporter, membrane spanning protein

Products: ADP; phosphate; S2O32- [Cytoplasm]; SO42- [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 285; Mature: 284

Protein sequence:

>285_residues
MSNNTSGNRWKWRQSSVIPGFGLTFGYTVTYLFLIILIPLGGLVWSTAKLGFADFIAIATDSRTLNALRVSFGTAFIAAL
VNAVFGVIVAWVLTRYRFPGRRFVDAIVDLPFALPTAVAGIALTTLYANRGWVGSLFEPFGIKIAFTPTGIVIALIFIGL
PFVVRTVQPVMEEIDRQVEEVAATLGANRFQTITRVLLPSLTPAILTGFALAFARGVGEYGSVIFIAGNIPYVSEIAPLL
IVIRLEEFNYAAATGIATIMLIISFAMLFLINLIQAWSRKRYGYV

Sequences:

>Translated_285_residues
MSNNTSGNRWKWRQSSVIPGFGLTFGYTVTYLFLIILIPLGGLVWSTAKLGFADFIAIATDSRTLNALRVSFGTAFIAAL
VNAVFGVIVAWVLTRYRFPGRRFVDAIVDLPFALPTAVAGIALTTLYANRGWVGSLFEPFGIKIAFTPTGIVIALIFIGL
PFVVRTVQPVMEEIDRQVEEVAATLGANRFQTITRVLLPSLTPAILTGFALAFARGVGEYGSVIFIAGNIPYVSEIAPLL
IVIRLEEFNYAAATGIATIMLIISFAMLFLINLIQAWSRKRYGYV
>Mature_284_residues
SNNTSGNRWKWRQSSVIPGFGLTFGYTVTYLFLIILIPLGGLVWSTAKLGFADFIAIATDSRTLNALRVSFGTAFIAALV
NAVFGVIVAWVLTRYRFPGRRFVDAIVDLPFALPTAVAGIALTTLYANRGWVGSLFEPFGIKIAFTPTGIVIALIFIGLP
FVVRTVQPVMEEIDRQVEEVAATLGANRFQTITRVLLPSLTPAILTGFALAFARGVGEYGSVIFIAGNIPYVSEIAPLLI
VIRLEEFNYAAATGIATIMLIISFAMLFLINLIQAWSRKRYGYV

Specific function: Part of the ABC transporter complex CysAWTP (TC 3.A.1.6.1) involved in sulfate/thiosulfate import. Probably responsible for the translocation of the substrate across the membrane [H]

COG id: COG0555

COG function: function code O; ABC-type sulfate transport system, permease component

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1788764, Length=269, Percent_Identity=46.8401486988848, Blast_Score=235, Evalue=2e-63,
Organism=Escherichia coli, GI87082099, Length=241, Percent_Identity=32.7800829875519, Blast_Score=136, Evalue=2e-33,
Organism=Escherichia coli, GI1786980, Length=223, Percent_Identity=34.0807174887892, Blast_Score=103, Evalue=1e-23,
Organism=Escherichia coli, GI1786254, Length=220, Percent_Identity=26.3636363636364, Blast_Score=86, Evalue=4e-18,
Organism=Escherichia coli, GI1787369, Length=210, Percent_Identity=26.6666666666667, Blast_Score=75, Evalue=7e-15,
Organism=Escherichia coli, GI1787368, Length=217, Percent_Identity=28.5714285714286, Blast_Score=68, Evalue=5e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515
- InterPro:   IPR011865
- InterPro:   IPR005667 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 31128; Mature: 30997

Theoretical pI: Translated: 10.22; Mature: 10.22

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
1.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNNTSGNRWKWRQSSVIPGFGLTFGYTVTYLFLIILIPLGGLVWSTAKLGFADFIAIAT
CCCCCCCCCEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHEEC
DSRTLNALRVSFGTAFIAALVNAVFGVIVAWVLTRYRFPGRRFVDAIVDLPFALPTAVAG
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCHHHHHHHHH
IALTTLYANRGWVGSLFEPFGIKIAFTPTGIVIALIFIGLPFVVRTVQPVMEEIDRQVEE
HHHHHHHHCCCCCCCCCCCCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VAATLGANRFQTITRVLLPSLTPAILTGFALAFARGVGEYGSVIFIAGNIPYVSEIAPLL
HHHHHCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCEEEEECCCCCHHHHHHHH
IVIRLEEFNYAAATGIATIMLIISFAMLFLINLIQAWSRKRYGYV
HHEEEHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure 
SNNTSGNRWKWRQSSVIPGFGLTFGYTVTYLFLIILIPLGGLVWSTAKLGFADFIAIAT
CCCCCCCCEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHEEC
DSRTLNALRVSFGTAFIAALVNAVFGVIVAWVLTRYRFPGRRFVDAIVDLPFALPTAVAG
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCHHHHHHHHH
IALTTLYANRGWVGSLFEPFGIKIAFTPTGIVIALIFIGLPFVVRTVQPVMEEIDRQVEE
HHHHHHHHCCCCCCCCCCCCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VAATLGANRFQTITRVLLPSLTPAILTGFALAFARGVGEYGSVIFIAGNIPYVSEIAPLL
HHHHHCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCEEEEECCCCCHHHHHHHH
IVIRLEEFNYAAATGIATIMLIISFAMLFLINLIQAWSRKRYGYV
HHEEEHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; S2O32- [Periplasm]; H2O; SO42- [Periplasm]; ATP [C]

Specific reaction: ATP + S2O32- [Periplasm] + H2O = ADP + phosphate + S2O32- [Cytoplasm] SO42- [Periplasm] + H2O + ATP = SO42- [Cytoplasm] + phosphate + ADP [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 2188958; 9205837; 9278503; 2188959 [H]