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The map label for this gene is cysD

Identifier: 159184475

GI number: 159184475

Start: 815511

End: 816464

Strand: Reverse

Name: cysD

Synonym: Atu0817

Alternate gene names: 159184475

Gene position: 816464-815511 (Counterclockwise)

Preceding gene: 159184476

Following gene: 15888157

Centisome position: 28.73

GC content: 59.43

Gene sequence:

>954_bases
ATGTCCAACGCAGTCCACGAGACGGACAGCAAGAATACTGTCGCATCCAAGCCGCCGCTCGATCCGCATCTGAAGGCGCT
TGAAAACGAAGCTATTCATATTTTCCGCGAAGTCGCCGCCGAATTCGATAACCCTGTGATGCTCTATTCCATCGGCAAGG
ACTCATCCGTGCTGCTGCATCTGGCGCGCAAGGCATTCTTTCCCGGGCGCATTCCCTTCCCGCTCCTGCACGTCAATACC
GGCTGGAAGTTCAAGGAAATGATCGAGTTCCGCGACAATATCGTGAAGGAATACGATCTCGACCTGATTTCCTACACCAA
CCCTCGCGGCGCGTTGGAAAACGTCACCCCCTTCTCCCATGGATCGGCGCTCTACACCGACATCATGAAGACGGAAGCGC
TGCGTCAGGCGCTGGATGCCGGCCAGTTCGACGCGGCATTCGGCGGCGCACGACGCGACGAAGAGGCGAGCCGCGCCAAG
GAACGAATCTATTCCTTCCGCACGCCCGACCATAAATGGGATCCGCGCAACCAGCGCCCGGAGCTGTGGAACATCTATAA
CGGCATGGTTCGCAAGGGCGAAAGCGTGCGCGCCTTCCCGCTTTCCAACTGGACCGAAGTCGATATCTGGCGTTACATCG
AGGCGGAAAACATTCCGCTGGTGCCGCTCTATTACGCTGCCGAGCGCCCCTATATCGAGCGCGACGGCATGATGATCCTT
GCCGAGGACGAGCGCCTAGAACTTCTGCCCGGTGAAGAGATCAAGCATGGCTCGATCCGCTTCCGCACGCTCGGCTGCTT
CCCGCTGACCGGCGCCATCCGCTCCGAGGCCGCAACGCTTCAGGACGTCATCGCCGAACTGGAAATCGCCACCGTCTCCG
AGCGGCAGGGCCGAGCGATTGACCGCGACCAATCCGGCTCCATGGAGAAAAAGAAGCGCGAGGGCTATTTCTGA

Upstream 100 bases:

>100_bases
AAGCGCGAATGCGGTCTTCACGTCGCCGGTGCGGAGCAGACACCCCCCGTTTCCGCCATCCCGCAACGATAATCATTCAG
TTCATCTCCCGGAGTTCACA

Downstream 100 bases:

>100_bases
TGAGCGCTGCAGCTGCCAACACCCCCTCCTCTTCCGCCACCATCCTGCCCTTTGCAGAACATTCGAAGGTGGCGCGCGAC
ACGCGTCCGCTGCGCCTTAT

Product: sulfate adenylyltransferase subunit 2

Products: NA

Alternate protein names: ATP-sulfurylase small subunit; Sulfate adenylate transferase; SAT

Number of amino acids: Translated: 317; Mature: 316

Protein sequence:

>317_residues
MSNAVHETDSKNTVASKPPLDPHLKALENEAIHIFREVAAEFDNPVMLYSIGKDSSVLLHLARKAFFPGRIPFPLLHVNT
GWKFKEMIEFRDNIVKEYDLDLISYTNPRGALENVTPFSHGSALYTDIMKTEALRQALDAGQFDAAFGGARRDEEASRAK
ERIYSFRTPDHKWDPRNQRPELWNIYNGMVRKGESVRAFPLSNWTEVDIWRYIEAENIPLVPLYYAAERPYIERDGMMIL
AEDERLELLPGEEIKHGSIRFRTLGCFPLTGAIRSEAATLQDVIAELEIATVSERQGRAIDRDQSGSMEKKKREGYF

Sequences:

>Translated_317_residues
MSNAVHETDSKNTVASKPPLDPHLKALENEAIHIFREVAAEFDNPVMLYSIGKDSSVLLHLARKAFFPGRIPFPLLHVNT
GWKFKEMIEFRDNIVKEYDLDLISYTNPRGALENVTPFSHGSALYTDIMKTEALRQALDAGQFDAAFGGARRDEEASRAK
ERIYSFRTPDHKWDPRNQRPELWNIYNGMVRKGESVRAFPLSNWTEVDIWRYIEAENIPLVPLYYAAERPYIERDGMMIL
AEDERLELLPGEEIKHGSIRFRTLGCFPLTGAIRSEAATLQDVIAELEIATVSERQGRAIDRDQSGSMEKKKREGYF
>Mature_316_residues
SNAVHETDSKNTVASKPPLDPHLKALENEAIHIFREVAAEFDNPVMLYSIGKDSSVLLHLARKAFFPGRIPFPLLHVNTG
WKFKEMIEFRDNIVKEYDLDLISYTNPRGALENVTPFSHGSALYTDIMKTEALRQALDAGQFDAAFGGARRDEEASRAKE
RIYSFRTPDHKWDPRNQRPELWNIYNGMVRKGESVRAFPLSNWTEVDIWRYIEAENIPLVPLYYAAERPYIERDGMMILA
EDERLELLPGEEIKHGSIRFRTLGCFPLTGAIRSEAATLQDVIAELEIATVSERQGRAIDRDQSGSMEKKKREGYF

Specific function: First step in the sulfate activation pathway. This reaction occurs early in the reductive branch of the cysteine biosynthetic pathway. [C]

COG id: COG0175

COG function: function code EH; 3'-phosphoadenosine 5'-phosphosulfate sulfotransferase (PAPS reductase)/FAD synthetase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PAPS reductase family. CysD subfamily

Homologues:

Organism=Escherichia coli, GI1789109, Length=295, Percent_Identity=68.135593220339, Blast_Score=433, Evalue=1e-123,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): CYSD_AGRT5 (Q7D0L8)

Other databases:

- EMBL:   AE007869
- RefSeq:   NP_353839.2
- HSSP:   Q87WW0
- ProteinModelPortal:   Q7D0L8
- SMR:   Q7D0L8
- STRING:   Q7D0L8
- GeneID:   1132855
- GenomeReviews:   AE007869_GR
- KEGG:   atu:Atu0817
- eggNOG:   COG0175
- HOGENOM:   HBG758022
- OMA:   SNAVHET
- ProtClustDB:   PRK05253
- HAMAP:   MF_00064
- InterPro:   IPR002500
- InterPro:   IPR014729
- InterPro:   IPR011784
- Gene3D:   G3DSA:3.40.50.620
- PIRSF:   PIRSF002936
- TIGRFAMs:   TIGR02039

Pfam domain/function: PF01507 PAPS_reduct

EC number: =2.7.7.4

Molecular weight: Translated: 36293; Mature: 36161

Theoretical pI: Translated: 5.69; Mature: 5.69

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNAVHETDSKNTVASKPPLDPHLKALENEAIHIFREVAAEFDNPVMLYSIGKDSSVLLH
CCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCHHHHH
LARKAFFPGRIPFPLLHVNTGWKFKEMIEFRDNIVKEYDLDLISYTNPRGALENVTPFSH
HHHHHCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHCCCEEEECCCCCCCCCCCCCCC
GSALYTDIMKTEALRQALDAGQFDAAFGGARRDEEASRAKERIYSFRTPDHKWDPRNQRP
CCHHHHHHHHHHHHHHHHCCCCCHHHHCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCC
ELWNIYNGMVRKGESVRAFPLSNWTEVDIWRYIEAENIPLVPLYYAAERPYIERDGMMIL
HHHHHHHHHHHCCCCEEEEECCCCCEEHEEEEEECCCCCEEEEEECCCCCEEECCCEEEE
AEDERLELLPGEEIKHGSIRFRTLGCFPLTGAIRSEAATLQDVIAELEIATVSERQGRAI
ECCCCEECCCCCCCCCCCEEEEEECCEECCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
DRDQSGSMEKKKREGYF
CCCCCCCHHHHHHCCCC
>Mature Secondary Structure 
SNAVHETDSKNTVASKPPLDPHLKALENEAIHIFREVAAEFDNPVMLYSIGKDSSVLLH
CCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCHHHHH
LARKAFFPGRIPFPLLHVNTGWKFKEMIEFRDNIVKEYDLDLISYTNPRGALENVTPFSH
HHHHHCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHCCCEEEECCCCCCCCCCCCCCC
GSALYTDIMKTEALRQALDAGQFDAAFGGARRDEEASRAKERIYSFRTPDHKWDPRNQRP
CCHHHHHHHHHHHHHHHHCCCCCHHHHCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCC
ELWNIYNGMVRKGESVRAFPLSNWTEVDIWRYIEAENIPLVPLYYAAERPYIERDGMMIL
HHHHHHHHHHHCCCCEEEEECCCCCEEHEEEEEECCCCCEEEEEECCCCCEEECCCEEEE
AEDERLELLPGEEIKHGSIRFRTLGCFPLTGAIRSEAATLQDVIAELEIATVSERQGRAI
ECCCCEECCCCCCCCCCCEEEEEECCEECCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
DRDQSGSMEKKKREGYF
CCCCCCCHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11743193; 11743194