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The map label for this gene is cysN

Identifier: 15888157

GI number: 15888157

Start: 814027

End: 815511

Strand: Reverse

Name: cysN

Synonym: Atu0816

Alternate gene names: 15888157

Gene position: 815511-814027 (Counterclockwise)

Preceding gene: 159184475

Following gene: 15888155

Centisome position: 28.7

GC content: 63.5

Gene sequence:

>1485_bases
ATGAGCGCTGCAGCTGCCAACACCCCCTCCTCTTCCGCCACCATCCTGCCCTTTGCAGAACATTCGAAGGTGGCGCGCGA
CACGCGTCCGCTGCGCCTTATCACCTGCGGCAGCGTGGATGATGGCAAATCGACCCTGATCGGCCGTCTCCTCTGGGACA
CCAAGGCCGTCAAGGAAGATCAGGCCGCCACGTTGCACCGTGACAGCGGCAAGCAGAACGATCTCGGCCTGCCCGATTTC
GCACTGCTGCTTGATGGTTTGCAGGCCGAGCGCGAACAGGGCATCACCATCGATGTCGCCTATCGTTATTTCGCCACCGA
CCGGCGCGCCTTCATCGTCGCCGATACGCCCGGCCATGAACAATATACCCGCAACATGGCGACCGGCGCTTCCACGGCCG
ATCTCGCCGTGCTGCTGGTCGATGCGCGCACCGGCATTCTGGAACAGACCCGCCGCCACGCCACCATTGCGGCGCTGATG
GGCATCCGGCAATTCGTGCTGGCGGTCAACAAGATCGACCTGACGAATTACGACAAGGCCGGTTTCGAACTGATCGCCCA
CGAGTTCCGCGATTTCGCCTCCGATCTCGGCATCAAGCAGATCACCGCGATTCCGATGTCGGCGCTGAAGGGTGAAAACG
TCGTGCTGTCCGGCAAGGCCTCCATGCCCTGGTATGAAGGCCCGACGCTGGTGGAGACGCTGGAGCTTGCCACCGTCCGC
TCCACGCAGTCCGGTGGCTTCCGCCTGCCCGTACAGCGCGTGTCGCGGCCAGGTGAAAGCTTCCGCGGTTATCAAGGCAC
GGTTGCCGGCGGTTCGGTGAAGCCCGGCGACAGCGTTGTCGTCCTGCCCTCGGGCATGGTCGCCAATGTCAAGCAGATCG
TCACCTTCGATCTAGTGCGCAATGCCGCTGTCGCGGGTGATGCCGTCACGCTCGTGCTCGACCGTCAAGTGGATGTCTCC
CGCGGCGACATGATCGTTTCCATCGAGGCACAACCGCTCACGGGACTTGCTTTTGACGCGCAGATCGTCGCCCTGCAGCC
GGGCGGCATCGAGGCCGGCAAACGCTACTGGCTGAAAAGCGCCAGCCGCCGCCAGCGCGTCAGCGTCCAGCCTGTTAGCC
AGCTCAACCTTCGGGAAGGCGAATGGCAGGCGCATGAGACCTCGCTGCCGATGAACGCCATCGGCAAGGTGCGTCTCTCC
TTCGACGAGACGGCGATCTTCGATCCCTATGAACAGAACCGGGCGACCGGCTCCTTCATCCTGATCGACCCTGACACCAA
CAATACGGTGGCGGGCGGCATGATCTCGGCCAAGCGCAGCACAGGTGCGACGGAGGAACAGGGCGACCGCGTCATCCTCT
CCCTGCCTGCCGGTCTGGCGGAAAAGCTGCTGGCCGGAGAACTGCTCGCCAAGCACCGCGACGAGATCGACATCCGCCGC
ACGGATGCGGCGACAGCTTCACGGCTCATAGGCGATCTCGACTGA

Upstream 100 bases:

>100_bases
CCGAACTGGAAATCGCCACCGTCTCCGAGCGGCAGGGCCGAGCGATTGACCGCGACCAATCCGGCTCCATGGAGAAAAAG
AAGCGCGAGGGCTATTTCTG

Downstream 100 bases:

>100_bases
AAACGCCCACATGAACGGCAAAAGGCGACCACGGTGTGGTCGCCTTTTTGTTTGGTCTGTCGTCCAGATGCGACTTAAAA
CACGAACATATAAAGCAGAA

Product: sulfate adenylyltransferase subunit 1

Products: NA

Alternate protein names: ATP-sulfurylase large subunit; Sulfate adenylate transferase; SAT

Number of amino acids: Translated: 494; Mature: 493

Protein sequence:

>494_residues
MSAAAANTPSSSATILPFAEHSKVARDTRPLRLITCGSVDDGKSTLIGRLLWDTKAVKEDQAATLHRDSGKQNDLGLPDF
ALLLDGLQAEREQGITIDVAYRYFATDRRAFIVADTPGHEQYTRNMATGASTADLAVLLVDARTGILEQTRRHATIAALM
GIRQFVLAVNKIDLTNYDKAGFELIAHEFRDFASDLGIKQITAIPMSALKGENVVLSGKASMPWYEGPTLVETLELATVR
STQSGGFRLPVQRVSRPGESFRGYQGTVAGGSVKPGDSVVVLPSGMVANVKQIVTFDLVRNAAVAGDAVTLVLDRQVDVS
RGDMIVSIEAQPLTGLAFDAQIVALQPGGIEAGKRYWLKSASRRQRVSVQPVSQLNLREGEWQAHETSLPMNAIGKVRLS
FDETAIFDPYEQNRATGSFILIDPDTNNTVAGGMISAKRSTGATEEQGDRVILSLPAGLAEKLLAGELLAKHRDEIDIRR
TDAATASRLIGDLD

Sequences:

>Translated_494_residues
MSAAAANTPSSSATILPFAEHSKVARDTRPLRLITCGSVDDGKSTLIGRLLWDTKAVKEDQAATLHRDSGKQNDLGLPDF
ALLLDGLQAEREQGITIDVAYRYFATDRRAFIVADTPGHEQYTRNMATGASTADLAVLLVDARTGILEQTRRHATIAALM
GIRQFVLAVNKIDLTNYDKAGFELIAHEFRDFASDLGIKQITAIPMSALKGENVVLSGKASMPWYEGPTLVETLELATVR
STQSGGFRLPVQRVSRPGESFRGYQGTVAGGSVKPGDSVVVLPSGMVANVKQIVTFDLVRNAAVAGDAVTLVLDRQVDVS
RGDMIVSIEAQPLTGLAFDAQIVALQPGGIEAGKRYWLKSASRRQRVSVQPVSQLNLREGEWQAHETSLPMNAIGKVRLS
FDETAIFDPYEQNRATGSFILIDPDTNNTVAGGMISAKRSTGATEEQGDRVILSLPAGLAEKLLAGELLAKHRDEIDIRR
TDAATASRLIGDLD
>Mature_493_residues
SAAAANTPSSSATILPFAEHSKVARDTRPLRLITCGSVDDGKSTLIGRLLWDTKAVKEDQAATLHRDSGKQNDLGLPDFA
LLLDGLQAEREQGITIDVAYRYFATDRRAFIVADTPGHEQYTRNMATGASTADLAVLLVDARTGILEQTRRHATIAALMG
IRQFVLAVNKIDLTNYDKAGFELIAHEFRDFASDLGIKQITAIPMSALKGENVVLSGKASMPWYEGPTLVETLELATVRS
TQSGGFRLPVQRVSRPGESFRGYQGTVAGGSVKPGDSVVVLPSGMVANVKQIVTFDLVRNAAVAGDAVTLVLDRQVDVSR
GDMIVSIEAQPLTGLAFDAQIVALQPGGIEAGKRYWLKSASRRQRVSVQPVSQLNLREGEWQAHETSLPMNAIGKVRLSF
DETAIFDPYEQNRATGSFILIDPDTNNTVAGGMISAKRSTGATEEQGDRVILSLPAGLAEKLLAGELLAKHRDEIDIRRT
DAATASRLIGDLD

Specific function: May be the GTPase, regulating ATP sulfurylase activity

COG id: COG2895

COG function: function code P; GTPases - Sulfate adenylate transferase subunit 1

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. CysN/nodQ subfamily

Homologues:

Organism=Homo sapiens, GI4503471, Length=359, Percent_Identity=32.3119777158774, Blast_Score=156, Evalue=3e-38,
Organism=Homo sapiens, GI5729864, Length=297, Percent_Identity=33.3333333333333, Blast_Score=152, Evalue=9e-37,
Organism=Homo sapiens, GI223555963, Length=297, Percent_Identity=33.3333333333333, Blast_Score=152, Evalue=9e-37,
Organism=Homo sapiens, GI4503475, Length=359, Percent_Identity=30.6406685236769, Blast_Score=149, Evalue=7e-36,
Organism=Homo sapiens, GI194097354, Length=331, Percent_Identity=28.3987915407855, Blast_Score=134, Evalue=1e-31,
Organism=Homo sapiens, GI194018520, Length=331, Percent_Identity=28.3987915407855, Blast_Score=134, Evalue=1e-31,
Organism=Homo sapiens, GI194018522, Length=331, Percent_Identity=28.3987915407855, Blast_Score=134, Evalue=2e-31,
Organism=Homo sapiens, GI46094014, Length=331, Percent_Identity=28.3987915407855, Blast_Score=132, Evalue=1e-30,
Organism=Homo sapiens, GI34147630, Length=317, Percent_Identity=30.9148264984227, Blast_Score=97, Evalue=5e-20,
Organism=Escherichia coli, GI1789108, Length=416, Percent_Identity=51.4423076923077, Blast_Score=393, Evalue=1e-110,
Organism=Escherichia coli, GI2367247, Length=315, Percent_Identity=24.4444444444444, Blast_Score=71, Evalue=2e-13,
Organism=Escherichia coli, GI1789737, Length=153, Percent_Identity=30.718954248366, Blast_Score=69, Evalue=6e-13,
Organism=Escherichia coli, GI1790412, Length=153, Percent_Identity=30.718954248366, Blast_Score=69, Evalue=6e-13,
Organism=Caenorhabditis elegans, GI17552884, Length=352, Percent_Identity=32.1022727272727, Blast_Score=152, Evalue=3e-37,
Organism=Caenorhabditis elegans, GI17569207, Length=352, Percent_Identity=32.1022727272727, Blast_Score=152, Evalue=3e-37,
Organism=Caenorhabditis elegans, GI32566629, Length=425, Percent_Identity=28.7058823529412, Blast_Score=150, Evalue=2e-36,
Organism=Caenorhabditis elegans, GI115532067, Length=292, Percent_Identity=30.8219178082192, Blast_Score=147, Evalue=1e-35,
Organism=Caenorhabditis elegans, GI115532065, Length=292, Percent_Identity=30.8219178082192, Blast_Score=147, Evalue=1e-35,
Organism=Caenorhabditis elegans, GI32566303, Length=345, Percent_Identity=29.5652173913043, Blast_Score=121, Evalue=1e-27,
Organism=Caenorhabditis elegans, GI32566301, Length=143, Percent_Identity=37.0629370629371, Blast_Score=97, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI25141371, Length=259, Percent_Identity=27.7992277992278, Blast_Score=84, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI17556456, Length=177, Percent_Identity=31.638418079096, Blast_Score=73, Evalue=3e-13,
Organism=Saccharomyces cerevisiae, GI6325337, Length=363, Percent_Identity=31.9559228650138, Blast_Score=161, Evalue=2e-40,
Organism=Saccharomyces cerevisiae, GI6319594, Length=363, Percent_Identity=31.9559228650138, Blast_Score=161, Evalue=2e-40,
Organism=Saccharomyces cerevisiae, GI6322937, Length=330, Percent_Identity=30.9090909090909, Blast_Score=156, Evalue=6e-39,
Organism=Saccharomyces cerevisiae, GI6320377, Length=338, Percent_Identity=29.2899408284024, Blast_Score=117, Evalue=3e-27,
Organism=Saccharomyces cerevisiae, GI6324761, Length=334, Percent_Identity=28.1437125748503, Blast_Score=89, Evalue=1e-18,
Organism=Drosophila melanogaster, GI24652838, Length=353, Percent_Identity=32.8611898016997, Blast_Score=159, Evalue=5e-39,
Organism=Drosophila melanogaster, GI17137572, Length=353, Percent_Identity=32.8611898016997, Blast_Score=159, Evalue=5e-39,
Organism=Drosophila melanogaster, GI45553807, Length=353, Percent_Identity=32.5779036827195, Blast_Score=152, Evalue=4e-37,
Organism=Drosophila melanogaster, GI45553816, Length=353, Percent_Identity=32.5779036827195, Blast_Score=152, Evalue=4e-37,
Organism=Drosophila melanogaster, GI24651721, Length=353, Percent_Identity=32.5779036827195, Blast_Score=152, Evalue=4e-37,
Organism=Drosophila melanogaster, GI17864154, Length=353, Percent_Identity=32.5779036827195, Blast_Score=152, Evalue=4e-37,
Organism=Drosophila melanogaster, GI17137380, Length=356, Percent_Identity=29.7752808988764, Blast_Score=144, Evalue=2e-34,
Organism=Drosophila melanogaster, GI45550900, Length=309, Percent_Identity=31.3915857605178, Blast_Score=135, Evalue=6e-32,
Organism=Drosophila melanogaster, GI281363316, Length=315, Percent_Identity=29.2063492063492, Blast_Score=99, Evalue=8e-21,
Organism=Drosophila melanogaster, GI17864358, Length=315, Percent_Identity=29.2063492063492, Blast_Score=99, Evalue=8e-21,
Organism=Drosophila melanogaster, GI19921738, Length=250, Percent_Identity=29.6, Blast_Score=90, Evalue=5e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): CYSN_AGRT5 (Q8UH69)

Other databases:

- EMBL:   AE007869
- PIR:   AH2676
- PIR:   F97458
- RefSeq:   NP_353838.1
- ProteinModelPortal:   Q8UH69
- STRING:   Q8UH69
- GeneID:   1132854
- GenomeReviews:   AE007869_GR
- KEGG:   atu:Atu0816
- eggNOG:   COG2895
- HOGENOM:   HBG307581
- OMA:   MELNDIA
- PhylomeDB:   Q8UH69
- ProtClustDB:   PRK05124
- BioCyc:   ATUM176299-1:ATU0816-MONOMER
- HAMAP:   MF_00062
- InterPro:   IPR000795
- InterPro:   IPR011779
- InterPro:   IPR009001
- InterPro:   IPR009000
- PRINTS:   PR00315
- TIGRFAMs:   TIGR02034

Pfam domain/function: PF00009 GTP_EFTU; SSF50465 Elong_init_C; SSF50447 Translat_factor

EC number: =2.7.7.4

Molecular weight: Translated: 53222; Mature: 53091

Theoretical pI: Translated: 5.98; Mature: 5.98

Prosite motif: PS00301 EFACTOR_GTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSAAAANTPSSSATILPFAEHSKVARDTRPLRLITCGSVDDGKSTLIGRLLWDTKAVKED
CCCCCCCCCCCCEEEEEEHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHCCC
QAATLHRDSGKQNDLGLPDFALLLDGLQAEREQGITIDVAYRYFATDRRAFIVADTPGHE
HHHHEECCCCCCCCCCCCHHHHHHHHHHHHHHCCCEEEEEEEEEECCCEEEEEEECCCCH
QYTRNMATGASTADLAVLLVDARTGILEQTRRHATIAALMGIRQFVLAVNKIDLTNYDKA
HHHHHHHCCCCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCHH
GFELIAHEFRDFASDLGIKQITAIPMSALKGENVVLSGKASMPWYEGPTLVETLELATVR
HHHHHHHHHHHHHHHCCCHHHHHCCHHHCCCCCEEEECCCCCCCCCCCHHHHHHHHHEEE
STQSGGFRLPVQRVSRPGESFRGYQGTVAGGSVKPGDSVVVLPSGMVANVKQIVTFDLVR
CCCCCCEECCHHHHCCCCHHHCCCCCEECCCCCCCCCCEEEECCCHHHHHHHHHHHHHHH
NAAVAGDAVTLVLDRQVDVSRGDMIVSIEAQPLTGLAFDAQIVALQPGGIEAGKRYWLKS
HHHCCCCEEEEEEECCCCCCCCCEEEEEECCCCCCEEECEEEEEECCCCCCHHHHHHHHC
ASRRQRVSVQPVSQLNLREGEWQAHETSLPMNAIGKVRLSFDETAIFDPYEQNRATGSFI
CCHHCCEECCCHHHCCCCCCCCCCCCCCCCHHHCEEEEEEECCCEECCCHHHCCCCCEEE
LIDPDTNNTVAGGMISAKRSTGATEEQGDRVILSLPAGLAEKLLAGELLAKHRDEIDIRR
EECCCCCCCEECCEEEECCCCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHCCCCCEEEE
TDAATASRLIGDLD
CCHHHHHHHHCCCC
>Mature Secondary Structure 
SAAAANTPSSSATILPFAEHSKVARDTRPLRLITCGSVDDGKSTLIGRLLWDTKAVKED
CCCCCCCCCCCEEEEEEHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHCCC
QAATLHRDSGKQNDLGLPDFALLLDGLQAEREQGITIDVAYRYFATDRRAFIVADTPGHE
HHHHEECCCCCCCCCCCCHHHHHHHHHHHHHHCCCEEEEEEEEEECCCEEEEEEECCCCH
QYTRNMATGASTADLAVLLVDARTGILEQTRRHATIAALMGIRQFVLAVNKIDLTNYDKA
HHHHHHHCCCCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCHH
GFELIAHEFRDFASDLGIKQITAIPMSALKGENVVLSGKASMPWYEGPTLVETLELATVR
HHHHHHHHHHHHHHHCCCHHHHHCCHHHCCCCCEEEECCCCCCCCCCCHHHHHHHHHEEE
STQSGGFRLPVQRVSRPGESFRGYQGTVAGGSVKPGDSVVVLPSGMVANVKQIVTFDLVR
CCCCCCEECCHHHHCCCCHHHCCCCCEECCCCCCCCCCEEEECCCHHHHHHHHHHHHHHH
NAAVAGDAVTLVLDRQVDVSRGDMIVSIEAQPLTGLAFDAQIVALQPGGIEAGKRYWLKS
HHHCCCCEEEEEEECCCCCCCCCEEEEEECCCCCCEEECEEEEEECCCCCCHHHHHHHHC
ASRRQRVSVQPVSQLNLREGEWQAHETSLPMNAIGKVRLSFDETAIFDPYEQNRATGSFI
CCHHCCEECCCHHHCCCCCCCCCCCCCCCCHHHCEEEEEEECCCEECCCHHHCCCCCEEE
LIDPDTNNTVAGGMISAKRSTGATEEQGDRVILSLPAGLAEKLLAGELLAKHRDEIDIRR
EECCCCCCCEECCEEEECCCCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHCCCCCEEEE
TDAATASRLIGDLD
CCHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11743193; 11743194